ERDDAP > info > bcodmo_dataset_755809

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Lab crustose coralline algae skeletal density fx temp and pCO2] - The density (mg CaCO3/cm^ 3) of the skeleton of Clathromorphum nereostratum, when assessed as function of increasing seawater temperature and pCO2 concentration (Ocean Acidification: Century Scale Impacts to Ecosystem Structure and Function of Aleutian Kelp Forests)		I   M	background			BCO-DMO	bcodmo_dataset_755809

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	To evaluate whether rates of calcification within C. nereostratum have changed or will change with ocean warming and acidification, we cultured C. nereostratum under experimental conditions mimicking past, present, and predicted future levels of ocean temperature and pCO2 in the region, then followed this four-month incubation period with measurements of skeletal density using micro-computed tomography (microCT). Small C. nereostratum colonies (~4-5 cm diameter) were live collected from Adak in 2015 and immediately transported to the Northeastern University Marine Science Center in Nahant, Massachusetts. There, all specimens were acclimated to laboratory conditions at 8.5 degrees C for two weeks, after which individual C. nereostratum colonies were attached to the underside of plastic Petri dishes using cyanoacrylate glue and then allowed to acclimate for an additional two weeks before being moved to experimental aquaria. Conditions were then incrementally modified to achieve target temperature and pCO2 levels (see below) over a one-week period. After reaching target conditions, each 42-L aquarium was dosed with 213 mL of calcein fluorescent dye (Western Chemicals Inc.), which was recirculated in the aquaria for three days and then flushed from the system. Coralline algae incorporate the dye into their skeleton, thus creating a distinct line that can be viewed via fluorescent microscopy to demark the region of new growth within each individual. We employed four pCO2 conditions and three temperatures that, while factorially crossed, spanned pre-industrial, present-day, and projected year 2100 conditions (assuming an IPCC \"business as usual\" carbon emissions scenario; Pachauri and Meyer 2014). More extreme temperature (12.5 degrees C) and pCO2 (2800 micro-atm) conditions were also employed in the broader experiment but were not included in our study because they are not predicted to occur until year 2500, or later. For each treatment, we set values to average summertime conditions, the time when ~75% of C. nereostratum growth occurs (Adey et al. 2013). All treatments (4 pCO2 concentrations x 3 temperatures, fully factorial) were housed on individual shelves and consisted of three 42-liter acrylic aquaria and one 65-liter sump (n = 3 tanks/treatment). The aquaria were connected to a sump via a common overflow and return line but were each independently and continuously replenished with new seawater\u2014thereby establishing them as true experimental replicates. The sump contained a filter box with a nylon mesh particle filter and activated carbon, a protein skimmer (Eshopps PSK-75), and a return pump, all of which was connected to a water chiller (Coralife 1/4HP). Filtered natural seawater was added via Darhor manual flow controllers at a rate of 50 mL/min/tank, resulting in full replacement of treatment water every ~21 hours - sufficiently fast to prevent material depletion of the dissolved constituents of the seawater yet slow enough to allow the mixed gases being sparged into the experimental treatments to approach equilibrium with the seawater. Mixed gases were sparged into each tank with 91 cm long flexible bubblers at the rate of ~1 L/min via Darhor needle-valve gas flow controllers. Two 12,000K LED light arrays (Ecoxotic Panorama, Pro 24V) were mounted above each tank and set to an irradiance that mirrored average summer daylight irradiance at 10 m depth in the Aleutian Islands (~258 micro-E m-2 s-1; 12 hr light:12 hr dark cycle). Over the course of the four-month experiment, we measured pH (Accumet AB15 pH meter with Accufet solid state probe), salinity (YSI3200 meter with K=10 conductivity electrode and temperature probe), and temperature (NIST traceable red spirit glass thermometer) in each tank every Monday, Wednesday, and Friday. The pCO2 of the gas mixtures was measured with a Qubit S151 infrared CO2 analyzer and calibrated with certified mixed CO2 from Airgas Incorporated. Every 10 days, we characterized the full carbonate system chemistry of the experimental treatments from measured total alkalinity, dissolved inorganic carbon, temperature, and salinity. For this, seawater samples were obtained in 250 mL borosilicate ground-glass-stoppered bottles and immediately poisoned with 100 micro-L of saturated HgCl2 solution to halt biological activity (Dickson et al. 2007). Total alkalinity was measured via closed-cell potentiometric Gran titration and dissolved inorganic carbon was measured with a UIC 5400 Coulometer on a VINDTA 3C (Marianda Incorporated) using Dickson certified seawater reference material. Seawater pCO2, pH, carbonate ion concentration ([CO32-]) bicarbonate ion concentration ([HCO3-]), aqueous CO2, and calcite saturation state were calculated with the program CO2SYS (Lewis and Wallace 1998), using Roy and colleague's (1993) values for the K1 and K2 carbonic acid constants, the Mucci (1983) value for the stoichiometric calcite solubility product, the seawater pH scale, and an atmospheric pressure of 1.015 atm. At the beginning of the experiment we measured the buoyant weight of each specimen. We then scrubbed each specimen with a toothbrush and reweighed it every month and at the end of the experiment. With each weighing, we also photographed the specimen with a ruler and Reef Watch coral bleaching card in the field of view. We then measured the 2-d surface area of the photographed specimens (Image J, NIH). At the end of the experiment, all coralline algae were sectioned with a diamond lapidary saw (Inland Craft SwapTop 6.5\u201d Diamond Trim Saw) and either frozen for genetic analysis or sectioned into 6 mm slices, rinsed in a series of two 90% Ethanol baths, and allowed to air dry for further examination of growth and skeletal density. We measured the density of the calcified skeleton deposited by C. nereostratum during the four-month laboratory experiment via micro-computed tomography (microCT); see Chan et al. (2017) for methods development and analytical setup. In brief, samples were scanned in a GE Locus RS-9 (General Electric Health Care, London, Ontario) x-ray microCT at an energy of 90kVp and tube current of 450 micro-A. Two frames, each 4500 ms in duration, were averaged at 900 projection angles over a 360-degree rotation of the gantry to produce data that was processed into a 3D image with 20 micron isotropic voxel spacing. Only specimens raised in the experimental temperature and pCO2 treatments employed in the feeding assay (6 treatments, n = 3 specimens/treatment) were studied. For each specimen, three cuboid regions of interest (ROI) were then selected, focusing on the region of new growth as indicated by the calcein mark. ROI size was similar for all measurements (1445-1575 voxels); however, dimensions were adjusted depending on the amount of accretion incurred and to avoid overlap with the epithallus or tissues deposited prior to the experiment. Grayscale thresholding to eliminate non-calcified tissue was unnecessary, given that conceptacles were not present in the newly deposited tissue and intracellular pore spaces (6 microns) are smaller than the microCT voxel size (20 microns) and were therefore not resolved. However, an analysis employing thresholding (Chan et al. 2017) produced virtually identical results. We quantified the skeletal density within each ROI by calculating the fractional mineral content of the ROI (i.e., fractional composition of each voxel that is CaCO3), converting each value to units of pure crystal calcite (physical density: 2.71 g/cm^3), then averaging over all voxels in the ROI.
attribute	NC_GLOBAL	awards_0_award_nid	String	526658
attribute	NC_GLOBAL	awards_0_award_number	String	PLR-1316141
attribute	NC_GLOBAL	awards_0_data_url	String	http://nsf.gov/awardsearch/showAward?AWD_ID=1316141
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Arctic Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF ARC
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	390
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Skeletal density of Clathromorphum nereostratum PI's: Steneck (Umaine), J. Estes (UCSC), D. Rasher (BLOS) version: 2019-02-013
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-02-14T13:53:41Z
attribute	NC_GLOBAL	date_modified	String	2019-02-25T20:51:25Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.755809.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/755809
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	CO2 coulometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	To measure dissolved inorganic carbon
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	755821
attribute	NC_GLOBAL	instruments_0_description	String	A CO2 coulometer semi-automatically controls the sample handling and extraction of CO2 from seawater samples. Samples are acidified and the CO2 gas is bubbled into a titration cell where CO2 is converted to hydroxyethylcarbonic acid which is then automatically titrated with a coulometrically-generated base to a colorimetric endpoint.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB12
attribute	NC_GLOBAL	instruments_0_instrument_name	String	CO2 Coulometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	507
attribute	NC_GLOBAL	instruments_0_supplied_name	String	UIC 5400 Coulometer on a VINDTA 3C
attribute	NC_GLOBAL	instruments_1_acronym	String	Salinometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	To measure salinity and temperature of tanks
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	755818
attribute	NC_GLOBAL	instruments_1_description	String	A salinometer is a device designed to measure the salinity, or dissolved salt content, of a solution.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Salinometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	677
attribute	NC_GLOBAL	instruments_1_supplied_name	String	YSI3200 meter with K=10 conductivity electrode and temperature probe
attribute	NC_GLOBAL	instruments_2_acronym	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	To measure pH of tanks
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	755817
attribute	NC_GLOBAL	instruments_2_description	String	An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms) This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	681
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Accumet AB15 pH meter with Accufet solid state probe
attribute	NC_GLOBAL	instruments_3_acronym	String	inorganic carbon and alkalinity analyser
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	755822
attribute	NC_GLOBAL	instruments_3_description	String	The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water.
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	686
attribute	NC_GLOBAL	instruments_4_acronym	String	MFC
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	755849
attribute	NC_GLOBAL	instruments_4_description	String	Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Mass Flow Controller
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	712
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Darhor manual flow controllers
attribute	NC_GLOBAL	instruments_5_acronym	String	CO2 Analyzer
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	To measure pCO2 in tanks
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	755820
attribute	NC_GLOBAL	instruments_5_description	String	Measures atmospheric carbon dioxide (CO2) concentration.
attribute	NC_GLOBAL	instruments_5_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/382/
attribute	NC_GLOBAL	instruments_5_instrument_name	String	CO2 Analyzer
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	491476
attribute	NC_GLOBAL	instruments_5_supplied_name	String	Qubit S151 infrared CO2 analyzer
attribute	NC_GLOBAL	instruments_6_acronym	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_6_dataset_instrument_description	String	To maintain temperature in tanks
attribute	NC_GLOBAL	instruments_6_dataset_instrument_nid	String	755845
attribute	NC_GLOBAL	instruments_6_description	String	Immersible or in-line liquid cooling device, usually with temperature control.
attribute	NC_GLOBAL	instruments_6_instrument_name	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_6_instrument_nid	String	522982
attribute	NC_GLOBAL	instruments_6_supplied_name	String	Coralife 1/4HP
attribute	NC_GLOBAL	instruments_7_acronym	String	CT Scanner
attribute	NC_GLOBAL	instruments_7_dataset_instrument_description	String	To produce 3D imagery of specimens
attribute	NC_GLOBAL	instruments_7_dataset_instrument_nid	String	755846
attribute	NC_GLOBAL	instruments_7_description	String	A CT scan makes use of computer-processed combinations of many X-ray measurements taken from different angles to produce cross-sectional (tomographic) images (virtual "slices") of specific areas of a scanned object.
attribute	NC_GLOBAL	instruments_7_instrument_name	String	Computerized Tomography (CT) Scanner
attribute	NC_GLOBAL	instruments_7_instrument_nid	String	707113
attribute	NC_GLOBAL	instruments_7_supplied_name	String	GE Locus RS-9 (General Electric Health Care, London, Ontario) x-ray microCT
attribute	NC_GLOBAL	instruments_8_dataset_instrument_description	String	To measure temperature in the tanks
attribute	NC_GLOBAL	instruments_8_dataset_instrument_nid	String	755819
attribute	NC_GLOBAL	instruments_8_instrument_name	String	Thermometer
attribute	NC_GLOBAL	instruments_8_instrument_nid	String	725867
attribute	NC_GLOBAL	instruments_8_supplied_name	String	NIST traceable red spirit glass thermometer
attribute	NC_GLOBAL	keywords	String	ave, bco, bco-dmo, biological, carbon, carbon dioxide, carbonate, chemical, cm3, co2, co3, data, dataset, dioxide, dmo, erddap, management, mg_CaCO3_cm3, oceanography, office, preliminary, replicate, roi, sample, sample_ID, tank, tank_pCO2_ave, tank_temp_ave, temperature, treatment, treatment_pCO2, treatment_temp
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/755809/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/755809
attribute	NC_GLOBAL	param_mapping	String	{'755809': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/755809/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Maine
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	U Maine DMC
attribute	NC_GLOBAL	people_0_person_name	String	Robert S. Steneck
attribute	NC_GLOBAL	people_0_person_nid	String	526659
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of California-Santa Cruz
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UC Santa Cruz
attribute	NC_GLOBAL	people_1_person_name	String	James Estes
attribute	NC_GLOBAL	people_1_person_nid	String	51389
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_2_person_name	String	Douglas B. Rasher
attribute	NC_GLOBAL	people_2_person_nid	String	480721
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	OA Kelp Forest Function
attribute	NC_GLOBAL	projects_0_acronym	String	OA Kelp Forest Function
attribute	NC_GLOBAL	projects_0_description	String	Extracted from the NSF award abstract: Marine calcifying organisms are most at risk to rapid ocean acidification (OA) in cold-water ecosystems. The investigators propose to determine if a globally unique and widespread calcareous alga in Alaska's Aleutian archipelago, Clathromorphum nereostratum, is threatened with extinction due to the combined effects of OA and food web alterations. C. nereostratum is a slow growing coralline alga that can live to at least 2000 years. It accretes massive 'bioherms' that dominate the regions' rocky substrate both under kelp forests and deforested sea urchin barrens. It develops growth bands (similar to tree rings) in its calcareous skeleton, which effectively record its annual calcification rate over centuries. Pilot data suggest the skeletal density of C. nereostratum began to decline precipitously in the 1990's in some parts of the Aleutian archipelago. The investigators now propose to use high-resolution microscopy and microCT imaging to examine how the growth and skeletal density of C. nereostratum has changed in the past 300 years (i.e., since the industrial revolution) across the western Aleutians. They will compare their records of algal skeletal densities and their variation through time with reconstructions of past climate to infer causes of change. In addition, the investigators will examine whether the alga's defense against grazing by sea urchins is compromised by ongoing ocean acidification. The investigators will survey the extent of C. nereostratum bioerosion occurring at 10 sites spanning the western Aleutians, both inside and outside of kelp forests. At each site they will compare these patterns to observed and monitored ecosystem trophic structure and recent C. nereostratum calcification rates. Field observations will be combined with laboratory experiments to determine if it is a decline in the alga's skeletal density (due to recent OA and warming), an increase in grazing intensity (due to recent trophic-level dysfunction), or their interactive effects that are likely responsible for bioerosion patterns inside vs. outside of forests. By sampling C. nereostratum inside and outside of forests, they will determine if kelp forests locally increase pH via photosynthesis, and thus buffer the effects of OA on coralline calcification. The combination of field observations with laboratory controlled experiments, manipulating CO2 and temperature, will help elucidate drivers of calcification and project how these species interactions will likely change in the near future. The project will provide the first in situ example of how ongoing ocean acidification is affecting the physiology of long-lived, carbonate producing organisms in the subarctic North Pacific. It will also be one of the first studies to document whether OA, ocean warming, and food web changes to ecological processes are interacting in complex ways to reshape the outcome of species interactions in nature.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-08
attribute	NC_GLOBAL	projects_0_name	String	Ocean Acidification: Century Scale Impacts to Ecosystem Structure and Function of Aleutian Kelp Forests
attribute	NC_GLOBAL	projects_0_project_nid	String	526660
attribute	NC_GLOBAL	projects_0_start_date	String	2013-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Skeletal density (mg CaCO3/cm^3) of Clathromorphum nereostratum, evaluated as a function of seawater temperature and pCO2 level that it was cultured in for 4 months in mesocosm. Density measurements were made using micro-computed tomography.
attribute	NC_GLOBAL	title	String	[Lab crustose coralline algae skeletal density fx temp and pCO2] - The density (mg CaCO3/cm^3) of the skeleton of Clathromorphum nereostratum, when assessed as function of increasing seawater temperature and pCO2 concentration (Ocean Acidification: Century Scale Impacts to Ecosystem Structure and Function of Aleutian Kelp Forests)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	treatment_temp		float
attribute	treatment_temp	_FillValue	float	NaN
attribute	treatment_temp	actual_range	float	6.5, 8.5
attribute	treatment_temp	bcodmo_name	String	treatment
attribute	treatment_temp	description	String	target temperature
attribute	treatment_temp	long_name	String	Treatment Temp
attribute	treatment_temp	units	String	degrees Celsius
variable	treatment_pCO2		short
attribute	treatment_pCO2	_FillValue	short	32767
attribute	treatment_pCO2	actual_range	short	330, 850
attribute	treatment_pCO2	bcodmo_name	String	treatment
attribute	treatment_pCO2	description	String	target pCO2 concentration
attribute	treatment_pCO2	long_name	String	Treatment P CO2
attribute	treatment_pCO2	units	String	microatmospheres (uatm)
variable	tank		byte
attribute	tank	_FillValue	byte	127
attribute	tank	actual_range	byte	1, 3
attribute	tank	bcodmo_name	String	replicate
attribute	tank	description	String	replicate tank
attribute	tank	long_name	String	Tank
attribute	tank	units	String	unitless
variable	tank_temp_ave		float
attribute	tank_temp_ave	_FillValue	float	NaN
attribute	tank_temp_ave	actual_range	float	6.33, 8.91
attribute	tank_temp_ave	bcodmo_name	String	temperature
attribute	tank_temp_ave	description	String	average tank temperature during study
attribute	tank_temp_ave	long_name	String	Tank Temp Ave
attribute	tank_temp_ave	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	tank_temp_ave	units	String	degrees Celsius
variable	tank_pCO2_ave		float
attribute	tank_pCO2_ave	_FillValue	float	NaN
attribute	tank_pCO2_ave	actual_range	float	323.1, 1110.49
attribute	tank_pCO2_ave	bcodmo_name	String	pCO2
attribute	tank_pCO2_ave	description	String	average tank pCO2 concentration during study
attribute	tank_pCO2_ave	long_name	String	Tank P CO2 Ave
attribute	tank_pCO2_ave	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	tank_pCO2_ave	units	String	microatmospheres (uatm)
variable	sample_ID		byte
attribute	sample_ID	_FillValue	byte	127
attribute	sample_ID	actual_range	byte	1, 32
attribute	sample_ID	bcodmo_name	String	sample
attribute	sample_ID	description	String	unique identifier for each algal sample
attribute	sample_ID	long_name	String	Sample ID
attribute	sample_ID	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample_ID	units	String	unitless
variable	replicate		byte
attribute	replicate	_FillValue	byte	127
attribute	replicate	actual_range	byte	1, 3
attribute	replicate	bcodmo_name	String	replicate
attribute	replicate	description	String	replicate coralline alga
attribute	replicate	long_name	String	Replicate
attribute	replicate	units	String	unitless
variable	ROI		byte
attribute	ROI	_FillValue	byte	127
attribute	ROI	actual_range	byte	1, 3
attribute	ROI	bcodmo_name	String	sample
attribute	ROI	description	String	region of interest scanned within each alga
attribute	ROI	long_name	String	ROI
attribute	ROI	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	ROI	units	String	unitless
variable	mg_CaCO3_cm3		float
attribute	mg_CaCO3_cm3	_FillValue	float	NaN
attribute	mg_CaCO3_cm3	actual_range	float	1218.6, 1791.3
attribute	mg_CaCO3_cm3	bcodmo_name	String	density
attribute	mg_CaCO3_cm3	description	String	skeletal (CaCO3) density within the ROI
attribute	mg_CaCO3_cm3	long_name	String	Mg Ca CO3 Cm3
attribute	mg_CaCO3_cm3	units	String	milligrams/cm^3

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.