ERDDAP > info > bcodmo_dataset_756510

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Breviolum antillogorgium cell counts] - Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018 (RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs)		I   M	background			BCO-DMO	bcodmo_dataset_756510

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Breviolum antillogorgium cultures were initially isolated from Antillogorgia bipinnata colonies collected at in the upper Florida Reef tract at Elbow Reef (N 25 07.956 W 80 15.810) in September 2016.\u00a0 Culture isolations and cell growth experimentation occurred in the Coffroth lab, University at Buffalo. A total of six Breviolum antillogorgium genotypes were used in this experiment. Three cultures had been grown at 26 degrees C since isolation in 2016 (16-0590F, 16-0875 and 16-1631) and three had been grown at 30 degrees C since isolation in 2016 (16-0587, 16-0763 and 16-0764). Three replicates of each of the six Breviolum antillogorgium cultures were grown at 26 and 30 C for a total of four treatments (26 degrees at 26 and 30 C and 30 degrees C at 26 and 30 C). A total of four to six replicate counts from each culture was made every three to twelve days using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated. Data Processing: Mean x 10000 were calculated from the counts.
attribute	NC_GLOBAL	awards_0_award_nid	String	658940
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1559286
attribute	NC_GLOBAL	awards_0_data_url	String	https://www.nsf.gov/awardsearch/showAward?AWD_ID=1559286
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Breviolum antillogorgium cell counts for growth rate: Oct - Dec 2018 PI's: C. terHorst (CSU), M.A. Coffroth (UBuffalo) version: 2019-02-20
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-02-21T13:58:43Z
attribute	NC_GLOBAL	date_modified	String	2019-03-04T19:24:58Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.756510.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/756510
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to make cell counts.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	756520
attribute	NC_GLOBAL	instruments_0_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Reichert Brightline hemocytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	756519
attribute	NC_GLOBAL	instruments_1_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	708
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Zeiss
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, count, Count_1, Count_2, Count_3, Count_4, Count_5, Count_6, culture, Culture_ID, data, dataset, date, day, dmo, erddap, management, mean, oceanography, office, preliminary, time
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/756510/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/756510
attribute	NC_GLOBAL	param_mapping	String	{'756510': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/756510/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	State University of New York at Buffalo
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	SUNY Buffalo
attribute	NC_GLOBAL	people_0_person_name	String	Mary Alice Coffroth
attribute	NC_GLOBAL	people_0_person_nid	String	472488
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	California State University Northridge
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	CSU-Northridge
attribute	NC_GLOBAL	people_1_person_name	String	Casey terHorst
attribute	NC_GLOBAL	people_1_person_nid	String	632541
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_2_person_nid	String	50396
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	Host Symbiont Temp Response
attribute	NC_GLOBAL	projects_0_acronym	String	Host Symbiont Temp Response
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules. The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-03
attribute	NC_GLOBAL	projects_0_geolocation	String	Florida Keys, Caribbean
attribute	NC_GLOBAL	projects_0_name	String	RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
attribute	NC_GLOBAL	projects_0_project_nid	String	632538
attribute	NC_GLOBAL	projects_0_start_date	String	2016-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26 and 30 degrees C and reciprocally grown at 26 and 30 degrees C
attribute	NC_GLOBAL	title	String	[Breviolum antillogorgium cell counts] - Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018 (RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Date		String
attribute	Date	bcodmo_name	String	date
attribute	Date	description	String	date of count formatted as yyyy-mm-dd
attribute	Date	long_name	String	Date
attribute	Date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date	source_name	String	Date
attribute	Date	time_precision	String	1970-01-01
attribute	Date	units	String	unitless
variable	Day		byte
attribute	Day	_FillValue	byte	127
attribute	Day	actual_range	byte	1, 83
attribute	Day	bcodmo_name	String	days
attribute	Day	description	String	Number of days since inoculation
attribute	Day	long_name	String	Day
attribute	Day	units	String	days
variable	Culture_ID		String
attribute	Culture_ID	bcodmo_name	String	sample
attribute	Culture_ID	description	String	Identification of sample given as culture name (16-0587; 16-0590F; 16-1631; etc.); treatment temperature (26 or 30 C) and replicate (1; 2; 3)
attribute	Culture_ID	long_name	String	Culture ID
attribute	Culture_ID	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Culture_ID	units	String	unitless
variable	Count_1		short
attribute	Count_1	_FillValue	short	32767
attribute	Count_1	actual_range	short	0, 402
attribute	Count_1	bcodmo_name	String	cell_concentration
attribute	Count_1	colorBarMaximum	double	100.0
attribute	Count_1	colorBarMinimum	double	0.0
attribute	Count_1	description	String	First replicate of culture counted
attribute	Count_1	long_name	String	Count 1
attribute	Count_1	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Count_2		short
attribute	Count_2	_FillValue	short	32767
attribute	Count_2	actual_range	short	0, 369
attribute	Count_2	bcodmo_name	String	cell_concentration
attribute	Count_2	colorBarMaximum	double	100.0
attribute	Count_2	colorBarMinimum	double	0.0
attribute	Count_2	description	String	Second replicate of culture counted
attribute	Count_2	long_name	String	Count 2
attribute	Count_2	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Count_3		short
attribute	Count_3	_FillValue	short	32767
attribute	Count_3	actual_range	short	0, 335
attribute	Count_3	bcodmo_name	String	cell_concentration
attribute	Count_3	colorBarMaximum	double	100.0
attribute	Count_3	colorBarMinimum	double	0.0
attribute	Count_3	description	String	Third replicate of culture counted
attribute	Count_3	long_name	String	Count 3
attribute	Count_3	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Count_4		short
attribute	Count_4	_FillValue	short	32767
attribute	Count_4	actual_range	short	0, 332
attribute	Count_4	bcodmo_name	String	cell_concentration
attribute	Count_4	colorBarMaximum	double	100.0
attribute	Count_4	colorBarMinimum	double	0.0
attribute	Count_4	description	String	Fourth replicate of culture counted
attribute	Count_4	long_name	String	Count 4
attribute	Count_4	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Count_5		short
attribute	Count_5	_FillValue	short	32767
attribute	Count_5	actual_range	short	0, 370
attribute	Count_5	bcodmo_name	String	cell_concentration
attribute	Count_5	colorBarMaximum	double	100.0
attribute	Count_5	colorBarMinimum	double	0.0
attribute	Count_5	description	String	Fifth replicate of culture counted
attribute	Count_5	long_name	String	Count 5
attribute	Count_5	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Count_6		short
attribute	Count_6	_FillValue	short	32767
attribute	Count_6	actual_range	short	0, 318
attribute	Count_6	bcodmo_name	String	cell_concentration
attribute	Count_6	colorBarMaximum	double	100.0
attribute	Count_6	colorBarMinimum	double	0.0
attribute	Count_6	description	String	Sixth replicate of culture counted
attribute	Count_6	long_name	String	Count 6
attribute	Count_6	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)
variable	Mean		float
attribute	Mean	_FillValue	float	NaN
attribute	Mean	actual_range	float	0.17, 279.5
attribute	Mean	bcodmo_name	String	cell_concentration
attribute	Mean	description	String	Mean of replicate counts
attribute	Mean	long_name	String	Mean
attribute	Mean	units	String	cells x 10^4/milliliter (cells x 10^4 ml-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.