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Title Sum-
Institution Dataset ID
     data   graph     files  public Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia
bipinnata by sample and treatment for cultures historically grown at 26C and 30C and
reciprocally grown at 26C and 30C, Oct - Dec 2018
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Breviolum antillogorgium cultures were initially isolated from Antillogorgia
bipinnata colonies collected at in the upper Florida Reef tract at Elbow Reef
(N 25 07.956 W 80 15.810) in September 2016.\u00a0 Culture isolations and cell
growth experimentation occurred in the Coffroth lab, University at Buffalo.

A total of six Breviolum antillogorgium genotypes were used in this
experiment. Three cultures had been grown at 26 degrees C since isolation in
2016 (16-0590F, 16-0875 and 16-1631) and three had been grown at 30 degrees C
since isolation in 2016 (16-0587, 16-0763 and 16-0764). Three replicates of
each of the six Breviolum antillogorgium cultures were grown at 26 and 30 C
for a total of four treatments (26 degrees at 26 and 30 C and 30 degrees C at
26 and 30 C). A total of four to six replicate counts from each culture was
made every three to twelve days using light microscopy and a hemocytometer.
Mean symbiont density per ml was calculated.

Data Processing: Mean x 10000 were calculated from the counts.
attribute NC_GLOBAL awards_0_award_nid String 658940
attribute NC_GLOBAL awards_0_award_number String OCE-1559286
attribute NC_GLOBAL awards_0_data_url String https://www.nsf.gov/awardsearch/showAward?AWD_ID=1559286 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Breviolum antillogorgium cell counts for growth rate: Oct - Dec 2018
PI's: C. terHorst (CSU), M.A. Coffroth (UBuffalo)
version: 2019-02-20
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-02-21T13:58:43Z
attribute NC_GLOBAL date_modified String 2019-03-04T19:24:58Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.756510.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/756510 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Hemocytometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to make cell counts.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 756520
attribute NC_GLOBAL instruments_0_description String A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
attribute NC_GLOBAL instruments_0_instrument_name String Hemocytometer
attribute NC_GLOBAL instruments_0_instrument_nid String 704
attribute NC_GLOBAL instruments_0_supplied_name String Reichert Brightline hemocytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 756519
attribute NC_GLOBAL instruments_1_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Microscope-Optical
attribute NC_GLOBAL instruments_1_instrument_nid String 708
attribute NC_GLOBAL instruments_1_supplied_name String Zeiss
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, count, Count_1, Count_2, Count_3, Count_4, Count_5, Count_6, culture, Culture_ID, data, dataset, date, day, dmo, erddap, management, mean, oceanography, office, preliminary, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/756510/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/756510 (external link)
attribute NC_GLOBAL param_mapping String {'756510': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/756510/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String State University of New York at Buffalo
attribute NC_GLOBAL people_0_affiliation_acronym String SUNY Buffalo
attribute NC_GLOBAL people_0_person_name String Mary Alice Coffroth
attribute NC_GLOBAL people_0_person_nid String 472488
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String California State University Northridge
attribute NC_GLOBAL people_1_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_1_person_name String Casey terHorst
attribute NC_GLOBAL people_1_person_nid String 632541
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Host Symbiont Temp Response
attribute NC_GLOBAL projects_0_acronym String Host Symbiont Temp Response
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.
The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.
attribute NC_GLOBAL projects_0_end_date String 2020-03
attribute NC_GLOBAL projects_0_geolocation String Florida Keys, Caribbean
attribute NC_GLOBAL projects_0_name String RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
attribute NC_GLOBAL projects_0_project_nid String 632538
attribute NC_GLOBAL projects_0_start_date String 2016-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26 and 30 degrees C and reciprocally grown at 26 and 30 degrees C
attribute NC_GLOBAL title String Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Date   String  
attribute Date bcodmo_name String date
attribute Date description String date of count formatted as yyyy-mm-dd
attribute Date long_name String Date
attribute Date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Date source_name String Date
attribute Date time_precision String 1970-01-01
attribute Date units String unitless
variable Day   byte  
attribute Day _FillValue byte 127
attribute Day actual_range byte 1, 83
attribute Day bcodmo_name String days
attribute Day description String Number of days since inoculation
attribute Day long_name String Day
attribute Day units String days
variable Culture_ID   String  
attribute Culture_ID bcodmo_name String sample
attribute Culture_ID description String Identification of sample given as culture name (16-0587; 16-0590F; 16-1631; etc.); treatment temperature (26 or 30 C) and replicate (1; 2; 3)
attribute Culture_ID long_name String Culture ID
attribute Culture_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Culture_ID units String unitless
variable Count_1   short  
attribute Count_1 _FillValue short 32767
attribute Count_1 actual_range short 0, 402
attribute Count_1 bcodmo_name String cell_concentration
attribute Count_1 colorBarMaximum double 100.0
attribute Count_1 colorBarMinimum double 0.0
attribute Count_1 description String First replicate of culture counted
attribute Count_1 long_name String Count 1
attribute Count_1 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Count_2   short  
attribute Count_2 _FillValue short 32767
attribute Count_2 actual_range short 0, 369
attribute Count_2 bcodmo_name String cell_concentration
attribute Count_2 colorBarMaximum double 100.0
attribute Count_2 colorBarMinimum double 0.0
attribute Count_2 description String Second replicate of culture counted
attribute Count_2 long_name String Count 2
attribute Count_2 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Count_3   short  
attribute Count_3 _FillValue short 32767
attribute Count_3 actual_range short 0, 335
attribute Count_3 bcodmo_name String cell_concentration
attribute Count_3 colorBarMaximum double 100.0
attribute Count_3 colorBarMinimum double 0.0
attribute Count_3 description String Third replicate of culture counted
attribute Count_3 long_name String Count 3
attribute Count_3 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Count_4   short  
attribute Count_4 _FillValue short 32767
attribute Count_4 actual_range short 0, 332
attribute Count_4 bcodmo_name String cell_concentration
attribute Count_4 colorBarMaximum double 100.0
attribute Count_4 colorBarMinimum double 0.0
attribute Count_4 description String Fourth replicate of culture counted
attribute Count_4 long_name String Count 4
attribute Count_4 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Count_5   short  
attribute Count_5 _FillValue short 32767
attribute Count_5 actual_range short 0, 370
attribute Count_5 bcodmo_name String cell_concentration
attribute Count_5 colorBarMaximum double 100.0
attribute Count_5 colorBarMinimum double 0.0
attribute Count_5 description String Fifth replicate of culture counted
attribute Count_5 long_name String Count 5
attribute Count_5 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Count_6   short  
attribute Count_6 _FillValue short 32767
attribute Count_6 actual_range short 0, 318
attribute Count_6 bcodmo_name String cell_concentration
attribute Count_6 colorBarMaximum double 100.0
attribute Count_6 colorBarMinimum double 0.0
attribute Count_6 description String Sixth replicate of culture counted
attribute Count_6 long_name String Count 6
attribute Count_6 units String cells x 10^4/milliliter (cells x 10^4 ml-1)
variable Mean   float  
attribute Mean _FillValue float NaN
attribute Mean actual_range float 0.17, 279.5
attribute Mean bcodmo_name String cell_concentration
attribute Mean description String Mean of replicate counts
attribute Mean long_name String Mean
attribute Mean units String cells x 10^4/milliliter (cells x 10^4 ml-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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