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Title Sum-
Institution Dataset ID
     data   graph     files  public Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and
T5 bacteriophage, 2018 and 2019.
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String All materials were cleaned by soaking overnight with heating (1.5%
Citrad\u00ae, by Decon Labs, Inc) in deionized water, rinsed in RO water, and
soaked in 10% TMG HCl (Fisher) in ultrapure water for 30 days, then rinsed
with ultrapure water, let dry in an AirClean 400 work station overnight, and
double-bagged in polyethylene bags (Mellett et al. 2018). M9 minimal media for
bacterial cultures was made using ultrapure water (18.2 M\u03a9 cm),
containing final concentrations of 33.7 mM Na2HPO4\u00b72H2O (Sigma-Aldrich
\u226599.0% Titration), 22 mM KH2PO4 (ACS Reagent \u226599% purity), 8.56 mM
NaCl (Certified ACS \u226599.0% purity), 18.7 mM NH4Cl (Fisher Scientific
\u226599.0% purity FCC), 0.1 M MgSO4 (Sigma-Aldrich \u226599.99% trace metal
purity), 0.1 M CaCl2 (Alfa Aesar from Fisher Scientific 99.99% metals basis),
1 \u00b5g/ml Thiamine HCl (Fisher Scientific 99% purity), and 0.5% Glucose
(Fisher Scientific 99% purity) in 1 L of Milli-Q (Kutter and Sulakvelidze
2004, Table 1;\u00a0see Supplemental Documents below).

M9 minimal media was spiked with 57FeSO4 (final concentration: 10 \u00b5M),
then filtered through a 0.02 \u00b5m Whatman Anotop syringe filter that had
been rinsed with Milli-Q; the first few drops of media were discarded (Sample
2). Some un-spiked media was reserved for bacterial pellet rinses (Sample 1).
A volume of 20 mL media was inoculated with a frozen culture of the E. coli
strain ZK126 (Betty Kutter, Evergreen State College) which was grown over
eight generations exclusively on M9 minimal media with 57FeSO4. The culture
was placed into a polyethylene bag and vented to avoid contamination, then
incubated while shaking overnight at 37 \u00b0C to reach late-logarithmic
growth. The following morning, three 45 mL aliquots of 10 \u00b5M 57FeSO4
spiked and 0.02 \u00b5m filtered media samples (one 45 mL blank sample
remained un-inoculated with E. coli) were weighed into 250 mL acid-cleaned
polycarbonate flasks. Each of the three aliquots were inoculated with 10% of
the overnight bacterial culture. The three bacterial cultures as well as the
blank samples were placed in a vented polyethylene bag and incubated while
shaking at 37 \u00b0C. Once the culture reached mid-logarithmic growth, as
indicated by an absorbance (OD600) \u00a0measured on a spectrophotometer
between 0.200-0.500 (Figure 1), the cultures were divided into 20 mL aliquots
(Figure 2, see Supplemental Documents, below).

To rinse excess 57Fe label from the surface of the bacterial cells, the
aliquoted cultures were transferred to a 50 mL falcon tube and centrifuged at
a speed of 6500 x g. The bacterial cells pelleted to the base of the tube, and
the supernatant was discarded. A volume of 10 mL of fresh Fe-less (not spiked
with 57FeSO4) 0.02 \u00b5m filtered media was added and vortexed for 1 minute.
The rinsed bacterial cells were again centrifuged 6500 x g for 5 minutes to
pellet, and the cell rinsing was repeated three times. The final bacterial
pellet was re-suspended in 20 mL of Fe-less media. Samples designated A, B,
and C were T4 phage lysates, samples D, E, and F remained uninfected by phage
and served as bacterial control cells that were burst open by treatment with
chloroform, and samples G, H, and I were blanks of Fe-less media. For all
rounds (Round 1, 2, 3, 4, and 5 samples A, B, and C (Figure 3) were infected
with 5 \u00b5l of T4 bacteriophage (Betty Kutter, Evergreen State College) at
a titer of 1.3 x 1011 phage/ml. For Rounds 2, 3, 4, and 5, samples M, N, and O
were infected with 5 \u00b5l of T5 bacteriophage (ATCC\u00ae 11303-B5\u2122)
at a titer of 8.2 x 1011 phage/ml. Samples D, E, and F (bacterial controls) as
well as G, H, and I (blanks) remained uninfected and instead were stored at 4
\u00b0C overnight to be used as phage-free lysis controls. Phage-infected
cultures A, B, and C were left shaking at 37 \u00b0C overnight.

On the next day of the experiment, the uninfected bacterial controls D, E, and
F were treated with 20% chloroform (Fisher Scientific, Mobile phase for HPLC
applications \u226599.8% purity) and vortexed for one minute to burst open the
bacterial cells without viral lysis. The necessity for this viral lysis-free
control is to account for colloids from within the bacterial cells that
contain 57Fe and would purify with T4 phage. For Round 5 all phages (A, B, C,
M, N, O) and blanks (G, H, I) were also treated with 20% chloroform and
vortexed for one minute. All the samples were then centrifuged in 50 mL Falcon
tubes at a speed of 9500 x g for 5 minutes to pellet the remaining bacterial
debris. The supernatant, which contained the T4 phage progeny for samples A,
B, and C and the T5 phage progeny for samples M, N, and O, was then filtered
using a 0.22 \u00b5m Sterivex PVDF syringe filter (EMD Millipore), with a
Milli-Q pre-rinse and the first few drops of sample discarded. The filtrate
was the fraction containing phage and any soluble or colloidal 57Fe within the
dissolved size fraction (<0.22 \u00b5m). The subsequent filtration of a
subsample of the dissolved fraction through a 0.02 \u00b5m Whatman Anotop
syringe filter (with a Milli-Q pre-rinse and the first few drops of sample
discarded) was collected for the soluble fraction (<0.02 \u00b5m). The
difference between the dissolved and the soluble fractions are used to
calculate iron within the colloidal fraction (0.2 \u00b5m-0.02 \u00b5m).

The phage within the dissolved fraction were further purified using a sucrose
cushion, which is a density-dependent technique used to concentrate and purify
phage by precipitating viral particles below a dense layer of sucrose (Hurwitz
et al. 2013). To do so, a 2.5 ml layer of 38% sucrose (Fisher Scientific) in
SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris-HCl in Milli-Q, pH 7.5 and 0.02
\u00b5m filtered) was added to the bottom of the ultracentrifuge tube (Beckman
Coulter), followed by 1 ml of sample and 10.5 ml of SM Buffer by carefully
tilting the tube so as not to disturb the dense sucrose layer. The samples
were spun in a Beckman Coulter SW40Ti swinging bucket ultracentrifuge, for 3
hours and 15 minutes at 175,000 x g (37,200 rpm). The sucrose and SM buffer
layers were then discarded, and the tubes were dried in a laminar flow clean
hood (Air Clean) for 20 minutes. The pelleted phages from samples A-C and M-O,
including any potential bacterial colloids of the same density as the phage as
accounted for in samples D-F, were then resuspended in 1 mL of SM Buffer.

All the samples were dialyzed using Float-A-Lyzer 100 kDa dialysis devices
(Fisher Scientific) in 1 L of dialysis buffer (10 mM NaCl, 50 mM Tris-Cl pH
8.0, 10 mM MgCl2) for a total of 6 buffer changes over 4 days. Bacterial and
viral counts were performed throughout for samples using SYBR nucleic acid
stain under epifluorescence microscopy (Noble & Furman 1998).

Metal concentrations were quantified using Element XR ICP-MS (Thermo) after
50-fold dilution with 5% nitric (Fisher Scientific, Optima) containing 10 ppb
rhodium as internal standard, and using external standard calibration curves.
Blank values after rhodium correction are listed in Table 2 (see Supplemental
Documents below).
attribute NC_GLOBAL awards_0_award_nid String 713366
attribute NC_GLOBAL awards_0_award_number String OCE-1722761
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1722761 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Fe-Labeling Experiment: E.coli and T4, T5
PI's: K. Buck
version date: 2019-10-10
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-03-04T16:24:51Z
attribute NC_GLOBAL date_modified String 2020-01-27T21:15:48Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.757485.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/757485 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String ICP Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure metal concentrations.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 757493
attribute NC_GLOBAL instruments_0_description String An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB15/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Inductively Coupled Plasma Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 530
attribute NC_GLOBAL instruments_0_supplied_name String ELEMENT XR High Resolution Inductively Coupled Plasma Mass Spectrometer
attribute NC_GLOBAL instruments_1_acronym String Spectrophotometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to measure bacterial cell concentrations.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 762163
attribute NC_GLOBAL instruments_1_description String An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Spectrophotometer
attribute NC_GLOBAL instruments_1_instrument_nid String 707
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to concentrate cells and separate bacteria from phage.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 762164
attribute NC_GLOBAL instruments_2_description String A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute NC_GLOBAL instruments_2_instrument_name String Centrifuge
attribute NC_GLOBAL instruments_2_instrument_nid String 629890
attribute NC_GLOBAL instruments_2_supplied_name String Beckman Coulter SW40Ti swinging bucket ultracentrifuge
attribute NC_GLOBAL keywords String atoms, bact, bact_cells_ml, bco, bco-dmo, biological, cells, chemical, Cu_63_nmol, data, dataset, date, description, dmo, erddap, expt, expt_round, Fe_56_nM, Fe_56_nmol, Fe_57_atoms_per_phage, Fe_57_nM, Fe_57_nmol, management, Ni_60_nmol, nmol, notes, notes_expt, oceanography, office, Pb_208_nmol, per, phage, phage_VPL_ml, preliminary, round, sample, time, volume, vpl, Zn_66_nmol
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/757485/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/757485 (external link)
attribute NC_GLOBAL param_mapping String {'757485': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/757485/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of South Florida
attribute NC_GLOBAL people_0_affiliation_acronym String USF
attribute NC_GLOBAL people_0_person_name String Mya Breitbart
attribute NC_GLOBAL people_0_person_nid String 51740
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of South Florida
attribute NC_GLOBAL people_1_affiliation_acronym String USF
attribute NC_GLOBAL people_1_person_name String Kristen N. Buck
attribute NC_GLOBAL people_1_person_nid String 51624
attribute NC_GLOBAL people_1_role String Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of South Florida
attribute NC_GLOBAL people_2_affiliation_acronym String USF
attribute NC_GLOBAL people_2_person_name String Chelsea Bonnain
attribute NC_GLOBAL people_2_person_nid String 732872
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String University of South Florida
attribute NC_GLOBAL people_3_affiliation_acronym String USF
attribute NC_GLOBAL people_3_person_name String Salvatore Caprara
attribute NC_GLOBAL people_3_person_nid String 732874
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Fe-Virus
attribute NC_GLOBAL projects_0_acronym String Fe-Virus
attribute NC_GLOBAL projects_0_description String Iron is an essential micronutrient for phytoplankton that is required for photosynthesis and respiration. Insufficient iron has been shown to limit phytoplankton growth in large regions of the surface ocean, and correspondingly, iron cycling is directly linked to carbon cycling in much of the marine environment. Nearly all iron in seawater (>99%) exists as complexes with organic molecules called ligands, which govern the concentration of iron dissolved in the water and the bioavailability of that iron to phytoplankton. However, despite the importance of iron-binding organic ligands, their sources and identities are largely unknown. Viruses, the majority of which are phages (viruses that infect bacteria), are extremely abundant in seawater and are in the same size fraction as dissolved iron. Recent evidence that non-marine phages contain iron as part of their structures has led to the proposal that marine phages may represent a previously overlooked class of organic iron-binding ligands. This project is determining the contribution of marine phages to dissolved iron pools and culture phage-host systems in the laboratory to determine if phages utilize bacterial iron-uptake receptors for infection in the manner of a Trojan horse. As the first study to examine the biogeochemical impact of trace elements contained within the structure of highly abundant marine phage particles, successful completion of the proposed research will be transformative for biological and chemical oceanography and have far-reaching implications for other fields, including human health where iron availability plays an important role in microbial pathogenesis. This project contributes to the multidisciplinary training of a graduate student and postdoctoral researcher. Research results will be disseminated through scientific publications and presentations, and the public will be educated about linkages between viruses and ocean chemistry via a hands-on exhibit for the annual St. Petersburg Science Festival.
Building upon evidence from non-marine model systems demonstrating the presence of iron ions in phage tail proteins and phage utilization of cell surface receptors for siderophore-bound iron, this project combines field and laboratory-based experiments to test the following three hypotheses regarding iron-virus interactions in the oceans: (1) Iron incorporated into phage tails originates from bacterial cell reserves, reducing the amount of iron available for remineralization upon lysis; (2) Phages constitute important iron-binding ligands in the oceans, accounting for a substantial portion of organically complexed colloidal dissolved iron; (3) Marine phages compete with siderophore-bound iron for uptake receptors on the bacterial cell surface and use iron in their tails as a Trojan horse for infection. Initial calculations predict that phages could account for up to 70% of the colloidal fraction of organically complexed dissolved iron in the surface ocean; therefore, this project is critical for advancing knowledge of trace-metal cycling as well as phage-host interactions. Additionally, if a portion of the cellular iron thought to be released from bacterial cells for remineralization following lysis is already incorporated into phage tails, then these findings will have significant implications for oceanic biogeochemical models. Through a combination of laboratory-based culture experiments and field sample measurements, this project could reveal the identity of a ubiquitous component of colloidal organic iron-binding ligands, modify the estimates of iron concentrations and species released through viral lysis, and potentially identify a novel receptor type for marine phage that may compete with the acquisition of siderophore-bound iron by host bacteria.
attribute NC_GLOBAL projects_0_end_date String 2019-01
attribute NC_GLOBAL projects_0_name String EAGER: Iron-Virus Interactions in the Ocean
attribute NC_GLOBAL projects_0_project_nid String 713367
attribute NC_GLOBAL projects_0_start_date String 2017-02
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This data was collected as part of a study investigating the source of iron to bacteriophage (phage for short, or viruses that infect and kill bacteria) progeny. Evidence from a phage that infects E. coli shows iron incorporated into the tail fiber structure. This study aims at identifying whether the source of the iron is environmental or bacterially derived. E. coli bacterial cultures were grown in minimal media spiked with 10 \u00b5M 57FeSO4 then infected with phage T4 or T5. The phages were purified by methods of centrifugation, filtration, density-dependent ultracentrifugation, and dialyzing. The resulting phage fractions were quantified by SYBR epifluorescence microscopy and metal concentrations were measured on an ELEMENT XR ICP-MS.
attribute NC_GLOBAL title String Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable date_utc   String  
attribute date_utc bcodmo_name String date_utc
attribute date_utc description String UTC date when media was filtered and the experiment initiated
attribute date_utc long_name String Date Utc
attribute date_utc source_name String date_utc
attribute date_utc time_precision String 1970-01-01
attribute date_utc units String unitless
variable expt_round   float  
attribute expt_round _FillValue float NaN
attribute expt_round actual_range float 1.0, 5.0
attribute expt_round bcodmo_name String exp_id
attribute expt_round description String round of experiment and sample analysis on the XR ICP-MS
attribute expt_round long_name String Expt Round
attribute expt_round units String unitless
variable sample   String  
attribute sample bcodmo_name String sample
attribute sample description String sample identifier: 1 for Fe-less (un-spiked) 0.02 um filtered M9 Minimal Media; 2 for 10 uM 57FeSO4 spiked 0.02 um filtered M9 minimal media; or 3 for 0.02 um filtered SM Buffer. Each stage of the experiment was designated sequentially as follows: 20’s for pelleted E. coli cultures grown to mid-logarithmic phase and rinsed three times in Fe-less media (no 57Fe spike) by centrifugation and re-suspension; 30’s for supernatant of pelleted bacteria (20’s); 40’s for unfiltered centrifuged supernatant of bacterial culture infected with phage overnight; 50’s for 0.22 um filtered dissolved fraction; 60’s for 0.02 um filtered soluble fraction; 70’s for bacterial samples treated with chloroform; 80’s for the supernatant (sample layer) above the sucrose cushion following ultracentrifugation; 90’s for sucrose cushion pellet re-suspended in 0.02 um filtered SM buffer; DIA for dialyzed samples post-dialysis over 6 buffer tank changes; and B for dialysis buffer: B1 pre-dialysis; B1-2 post-initial dialysis; B3-2 post-third dialysis buffer tank change; and B6-2 post- final dialysis buffer tank change. Samples were treated in triplicate: A; B; C for E. coli phage T4 samples; D; E; F for chloroform-lysed bacterial control samples; and G; H; I for Blanks; and M; N; O for E. coli phage T5 samples.
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable description   String  
attribute description bcodmo_name String brief_desc
attribute description description String description of phage purification step and type of sample
attribute description long_name String Description
attribute description units String unitless
variable Fe_56_nM   String  
attribute Fe_56_nM bcodmo_name String Fe
attribute Fe_56_nM description String Concentration of 56Fe as determined by XR ICP-MS
attribute Fe_56_nM long_name String Fe 56 N M
attribute Fe_56_nM units String nanoMolar
variable Fe_57_nM   String  
attribute Fe_57_nM bcodmo_name String Fe
attribute Fe_57_nM description String Concentration of 57Fe as determined by XR ICP-MS
attribute Fe_57_nM long_name String Fe 57 N M
attribute Fe_57_nM units String nanoMolar
variable volume   String  
attribute volume bcodmo_name String volume
attribute volume description String sample volume
attribute volume long_name String Volume
attribute volume units String liters
variable Fe_56_nmol   String  
attribute Fe_56_nmol bcodmo_name String Fe
attribute Fe_56_nmol description String 56Fe concentration as determined by XR ICP-MS
attribute Fe_56_nmol long_name String Fe 56 Nmol
attribute Fe_56_nmol units String nanomoles
variable Fe_57_nmol   String  
attribute Fe_57_nmol bcodmo_name String Fe
attribute Fe_57_nmol description String 57Fe concentration as determined by XR ICP-MS
attribute Fe_57_nmol long_name String Fe 57 Nmol
attribute Fe_57_nmol units String nanomoles
variable Cu_63_nmol   float  
attribute Cu_63_nmol _FillValue float NaN
attribute Cu_63_nmol actual_range float 6.03E-4, 265.0
attribute Cu_63_nmol bcodmo_name String Cu
attribute Cu_63_nmol description String 63Cu concentration as determined by XR ICP-MS
attribute Cu_63_nmol long_name String Cu 63 Nmol
attribute Cu_63_nmol units String nanomoles
variable Zn_66_nmol   float  
attribute Zn_66_nmol _FillValue float NaN
attribute Zn_66_nmol actual_range float 0.0, 13800.0
attribute Zn_66_nmol bcodmo_name String Zn
attribute Zn_66_nmol description String 66Zn concentration as determined by XR ICP-MS
attribute Zn_66_nmol long_name String Zn 66 Nmol
attribute Zn_66_nmol units String nanomoles
variable Ni_60_nmol   float  
attribute Ni_60_nmol _FillValue float NaN
attribute Ni_60_nmol actual_range float 2.19E-4, 1820.0
attribute Ni_60_nmol bcodmo_name String trace_metal_conc
attribute Ni_60_nmol description String 60Ni concentration as determined by XR ICP-MS
attribute Ni_60_nmol long_name String Ni 60 Nmol
attribute Ni_60_nmol nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Ni_60_nmol units String nanomoles
variable Pb_208_nmol   float  
attribute Pb_208_nmol _FillValue float NaN
attribute Pb_208_nmol actual_range float 5.05E-5, 340.0
attribute Pb_208_nmol bcodmo_name String trace_metal_conc
attribute Pb_208_nmol description String 208Pb concentration as determined by XR ICP-MS
attribute Pb_208_nmol long_name String Pb 208 Nmol
attribute Pb_208_nmol nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Pb_208_nmol units String nanomoles
variable bact_cells_ml   float  
attribute bact_cells_ml _FillValue float NaN
attribute bact_cells_ml actual_range float 0.344, 4.55E8
attribute bact_cells_ml bcodmo_name String bact_abundance
attribute bact_cells_ml description String SYBR epifluorescence bacterial counts
attribute bact_cells_ml long_name String Bact Cells Ml
attribute bact_cells_ml nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/BNTX (external link)
attribute bact_cells_ml units String cells/milliliter
variable phage_VPL_ml   double  
attribute phage_VPL_ml _FillValue double NaN
attribute phage_VPL_ml actual_range double 4.49E9, 4.62E11
attribute phage_VPL_ml bcodmo_name String cell_concentration
attribute phage_VPL_ml description String phage cell concentration
attribute phage_VPL_ml long_name String Phage VPL Ml
attribute phage_VPL_ml units String virus-like particles/milliliter
variable Fe_57_atoms_per_phage   float  
attribute Fe_57_atoms_per_phage _FillValue float NaN
attribute Fe_57_atoms_per_phage actual_range float 74.3, 3200.0
attribute Fe_57_atoms_per_phage bcodmo_name String Fe
attribute Fe_57_atoms_per_phage description String 57Fe content measured by XR ICP- MS; converted to moles; multiplied by Avogadro’s constant (6.022 x 1023 atoms/mol); and divided by number of phage in the sample
attribute Fe_57_atoms_per_phage long_name String Fe 57 Atoms Per Phage
attribute Fe_57_atoms_per_phage units String atoms
variable notes_expt   String  
attribute notes_expt bcodmo_name String comment
attribute notes_expt description String comments about samples
attribute notes_expt long_name String Notes Expt
attribute notes_expt units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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