ERDDAP > info > bcodmo_dataset_757485

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Fe-Labeling Expt: E.coli and phage T4 or T5] - Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019. (EAGER: Iron- Virus Interactions in the Ocean)		I   M	background			BCO-DMO	bcodmo_dataset_757485

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	All materials were cleaned by soaking overnight with heating (1.5% Citrad\u00ae, by Decon Labs, Inc) in deionized water, rinsed in RO water, and soaked in 10% TMG HCl (Fisher) in ultrapure water for 30 days, then rinsed with ultrapure water, let dry in an AirClean 400 work station overnight, and double-bagged in polyethylene bags (Mellett et al. 2018). M9 minimal media for bacterial cultures was made using ultrapure water (18.2 M\u03a9 cm), containing final concentrations of 33.7 mM Na2HPO4\u00b72H2O (Sigma-Aldrich \u226599.0% Titration), 22 mM KH2PO4 (ACS Reagent \u226599% purity), 8.56 mM NaCl (Certified ACS \u226599.0% purity), 18.7 mM NH4Cl (Fisher Scientific \u226599.0% purity FCC), 0.1 M MgSO4 (Sigma-Aldrich \u226599.99% trace metal purity), 0.1 M CaCl2 (Alfa Aesar from Fisher Scientific 99.99% metals basis), 1 \u00b5g/ml Thiamine HCl (Fisher Scientific 99% purity), and 0.5% Glucose (Fisher Scientific 99% purity) in 1 L of Milli-Q (Kutter and Sulakvelidze 2004, Table 1;\u00a0see Supplemental Documents below). M9 minimal media was spiked with 57FeSO4 (final concentration: 10 \u00b5M), then filtered through a 0.02 \u00b5m Whatman Anotop syringe filter that had been rinsed with Milli-Q; the first few drops of media were discarded (Sample 2). Some un-spiked media was reserved for bacterial pellet rinses (Sample 1). A volume of 20 mL media was inoculated with a frozen culture of the E. coli strain ZK126 (Betty Kutter, Evergreen State College) which was grown over eight generations exclusively on M9 minimal media with 57FeSO4. The culture was placed into a polyethylene bag and vented to avoid contamination, then incubated while shaking overnight at 37 \u00b0C to reach late-logarithmic growth. The following morning, three 45 mL aliquots of 10 \u00b5M 57FeSO4 spiked and 0.02 \u00b5m filtered media samples (one 45 mL blank sample remained un-inoculated with E. coli) were weighed into 250 mL acid-cleaned polycarbonate flasks. Each of the three aliquots were inoculated with 10% of the overnight bacterial culture. The three bacterial cultures as well as the blank samples were placed in a vented polyethylene bag and incubated while shaking at 37 \u00b0C. Once the culture reached mid-logarithmic growth, as indicated by an absorbance (OD600) \u00a0measured on a spectrophotometer between 0.200-0.500 (Figure 1), the cultures were divided into 20 mL aliquots (Figure 2, see Supplemental Documents, below). To rinse excess 57Fe label from the surface of the bacterial cells, the aliquoted cultures were transferred to a 50 mL falcon tube and centrifuged at a speed of 6500 x g. The bacterial cells pelleted to the base of the tube, and the supernatant was discarded. A volume of 10 mL of fresh Fe-less (not spiked with 57FeSO4) 0.02 \u00b5m filtered media was added and vortexed for 1 minute. The rinsed bacterial cells were again centrifuged 6500 x g for 5 minutes to pellet, and the cell rinsing was repeated three times. The final bacterial pellet was re-suspended in 20 mL of Fe-less media. Samples designated A, B, and C were T4 phage lysates, samples D, E, and F remained uninfected by phage and served as bacterial control cells that were burst open by treatment with chloroform, and samples G, H, and I were blanks of Fe-less media. For all rounds (Round 1, 2, 3, 4, and 5 samples A, B, and C (Figure 3) were infected with 5 \u00b5l of T4 bacteriophage (Betty Kutter, Evergreen State College) at a titer of 1.3 x 1011 phage/ml. For Rounds 2, 3, 4, and 5, samples M, N, and O were infected with 5 \u00b5l of T5 bacteriophage (ATCC\u00ae 11303-B5\u2122) at a titer of 8.2 x 1011 phage/ml. Samples D, E, and F (bacterial controls) as well as G, H, and I (blanks) remained uninfected and instead were stored at 4 \u00b0C overnight to be used as phage-free lysis controls. Phage-infected cultures A, B, and C were left shaking at 37 \u00b0C overnight. On the next day of the experiment, the uninfected bacterial controls D, E, and F were treated with 20% chloroform (Fisher Scientific, Mobile phase for HPLC applications \u226599.8% purity) and vortexed for one minute to burst open the bacterial cells without viral lysis. The necessity for this viral lysis-free control is to account for colloids from within the bacterial cells that contain 57Fe and would purify with T4 phage. For Round 5 all phages (A, B, C, M, N, O) and blanks (G, H, I) were also treated with 20% chloroform and vortexed for one minute. All the samples were then centrifuged in 50 mL Falcon tubes at a speed of 9500 x g for 5 minutes to pellet the remaining bacterial debris. The supernatant, which contained the T4 phage progeny for samples A, B, and C and the T5 phage progeny for samples M, N, and O, was then filtered using a 0.22 \u00b5m Sterivex PVDF syringe filter (EMD Millipore), with a Milli-Q pre-rinse and the first few drops of sample discarded. The filtrate was the fraction containing phage and any soluble or colloidal 57Fe within the dissolved size fraction (<0.22 \u00b5m). The subsequent filtration of a subsample of the dissolved fraction through a 0.02 \u00b5m Whatman Anotop syringe filter (with a Milli-Q pre-rinse and the first few drops of sample discarded) was collected for the soluble fraction (<0.02 \u00b5m). The difference between the dissolved and the soluble fractions are used to calculate iron within the colloidal fraction (0.2 \u00b5m-0.02 \u00b5m). The phage within the dissolved fraction were further purified using a sucrose cushion, which is a density-dependent technique used to concentrate and purify phage by precipitating viral particles below a dense layer of sucrose (Hurwitz et al. 2013). To do so, a 2.5 ml layer of 38% sucrose (Fisher Scientific) in SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris-HCl in Milli-Q, pH 7.5 and 0.02 \u00b5m filtered) was added to the bottom of the ultracentrifuge tube (Beckman Coulter), followed by 1 ml of sample and 10.5 ml of SM Buffer by carefully tilting the tube so as not to disturb the dense sucrose layer. The samples were spun in a Beckman Coulter SW40Ti swinging bucket ultracentrifuge, for 3 hours and 15 minutes at 175,000 x g (37,200 rpm). The sucrose and SM buffer layers were then discarded, and the tubes were dried in a laminar flow clean hood (Air Clean) for 20 minutes. The pelleted phages from samples A-C and M-O, including any potential bacterial colloids of the same density as the phage as accounted for in samples D-F, were then resuspended in 1 mL of SM Buffer. All the samples were dialyzed using Float-A-Lyzer 100 kDa dialysis devices (Fisher Scientific) in 1 L of dialysis buffer (10 mM NaCl, 50 mM Tris-Cl pH 8.0, 10 mM MgCl2) for a total of 6 buffer changes over 4 days. Bacterial and viral counts were performed throughout for samples using SYBR nucleic acid stain under epifluorescence microscopy (Noble & Furman 1998). Metal concentrations were quantified using Element XR ICP-MS (Thermo) after 50-fold dilution with 5% nitric (Fisher Scientific, Optima) containing 10 ppb rhodium as internal standard, and using external standard calibration curves. Blank values after rhodium correction are listed in Table 2 (see Supplemental Documents below).
attribute	NC_GLOBAL	awards_0_award_nid	String	713366
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1722761
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1722761
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Fe-Labeling Experiment: E.coli and T4, T5 PI's: K. Buck version date: 2019-10-10
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-03-04T16:24:51Z
attribute	NC_GLOBAL	date_modified	String	2020-01-27T21:15:48Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.757485.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/757485
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	ICP Mass Spec
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to measure metal concentrations.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	757493
attribute	NC_GLOBAL	instruments_0_description	String	An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB15/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Inductively Coupled Plasma Mass Spectrometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	530
attribute	NC_GLOBAL	instruments_0_supplied_name	String	ELEMENT XR High Resolution Inductively Coupled Plasma Mass Spectrometer
attribute	NC_GLOBAL	instruments_1_acronym	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to measure bacterial cell concentrations.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	762163
attribute	NC_GLOBAL	instruments_1_description	String	An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB20/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	707
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Used to concentrate cells and separate bacteria from phage.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	762164
attribute	NC_GLOBAL	instruments_2_description	String	A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Centrifuge
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	629890
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Beckman Coulter SW40Ti swinging bucket ultracentrifuge
attribute	NC_GLOBAL	keywords	String	atoms, bact, bact_cells_ml, bco, bco-dmo, biological, cells, chemical, Cu_63_nmol, data, dataset, date, description, dmo, erddap, expt, expt_round, Fe_56_nM, Fe_56_nmol, Fe_57_atoms_per_phage, Fe_57_nM, Fe_57_nmol, management, Ni_60_nmol, nmol, notes, notes_expt, oceanography, office, Pb_208_nmol, per, phage, phage_VPL_ml, preliminary, round, sample, time, volume, vpl, Zn_66_nmol
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/757485/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/757485
attribute	NC_GLOBAL	param_mapping	String	{'757485': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/757485/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of South Florida
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	USF
attribute	NC_GLOBAL	people_0_person_name	String	Mya Breitbart
attribute	NC_GLOBAL	people_0_person_nid	String	51740
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of South Florida
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	USF
attribute	NC_GLOBAL	people_1_person_name	String	Kristen N. Buck
attribute	NC_GLOBAL	people_1_person_nid	String	51624
attribute	NC_GLOBAL	people_1_role	String	Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of South Florida
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	USF
attribute	NC_GLOBAL	people_2_person_name	String	Chelsea Bonnain
attribute	NC_GLOBAL	people_2_person_nid	String	732872
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	University of South Florida
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	USF
attribute	NC_GLOBAL	people_3_person_name	String	Salvatore Caprara
attribute	NC_GLOBAL	people_3_person_nid	String	732874
attribute	NC_GLOBAL	people_3_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_3_role_type	String	originator
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_4_person_nid	String	50396
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Fe-Virus
attribute	NC_GLOBAL	projects_0_acronym	String	Fe-Virus
attribute	NC_GLOBAL	projects_0_description	String	Iron is an essential micronutrient for phytoplankton that is required for photosynthesis and respiration. Insufficient iron has been shown to limit phytoplankton growth in large regions of the surface ocean, and correspondingly, iron cycling is directly linked to carbon cycling in much of the marine environment. Nearly all iron in seawater (>99%) exists as complexes with organic molecules called ligands, which govern the concentration of iron dissolved in the water and the bioavailability of that iron to phytoplankton. However, despite the importance of iron-binding organic ligands, their sources and identities are largely unknown. Viruses, the majority of which are phages (viruses that infect bacteria), are extremely abundant in seawater and are in the same size fraction as dissolved iron. Recent evidence that non-marine phages contain iron as part of their structures has led to the proposal that marine phages may represent a previously overlooked class of organic iron-binding ligands. This project is determining the contribution of marine phages to dissolved iron pools and culture phage-host systems in the laboratory to determine if phages utilize bacterial iron-uptake receptors for infection in the manner of a Trojan horse. As the first study to examine the biogeochemical impact of trace elements contained within the structure of highly abundant marine phage particles, successful completion of the proposed research will be transformative for biological and chemical oceanography and have far-reaching implications for other fields, including human health where iron availability plays an important role in microbial pathogenesis. This project contributes to the multidisciplinary training of a graduate student and postdoctoral researcher. Research results will be disseminated through scientific publications and presentations, and the public will be educated about linkages between viruses and ocean chemistry via a hands-on exhibit for the annual St. Petersburg Science Festival. Building upon evidence from non-marine model systems demonstrating the presence of iron ions in phage tail proteins and phage utilization of cell surface receptors for siderophore-bound iron, this project combines field and laboratory-based experiments to test the following three hypotheses regarding iron-virus interactions in the oceans: (1) Iron incorporated into phage tails originates from bacterial cell reserves, reducing the amount of iron available for remineralization upon lysis; (2) Phages constitute important iron-binding ligands in the oceans, accounting for a substantial portion of organically complexed colloidal dissolved iron; (3) Marine phages compete with siderophore-bound iron for uptake receptors on the bacterial cell surface and use iron in their tails as a Trojan horse for infection. Initial calculations predict that phages could account for up to 70% of the colloidal fraction of organically complexed dissolved iron in the surface ocean; therefore, this project is critical for advancing knowledge of trace-metal cycling as well as phage-host interactions. Additionally, if a portion of the cellular iron thought to be released from bacterial cells for remineralization following lysis is already incorporated into phage tails, then these findings will have significant implications for oceanic biogeochemical models. Through a combination of laboratory-based culture experiments and field sample measurements, this project could reveal the identity of a ubiquitous component of colloidal organic iron-binding ligands, modify the estimates of iron concentrations and species released through viral lysis, and potentially identify a novel receptor type for marine phage that may compete with the acquisition of siderophore-bound iron by host bacteria.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-01
attribute	NC_GLOBAL	projects_0_name	String	EAGER: Iron-Virus Interactions in the Ocean
attribute	NC_GLOBAL	projects_0_project_nid	String	713367
attribute	NC_GLOBAL	projects_0_start_date	String	2017-02
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This data was collected as part of a study investigating the source of iron to bacteriophage (phage for short, or viruses that infect and kill bacteria) progeny. Evidence from a phage that infects E. coli shows iron incorporated into the tail fiber structure. This study aims at identifying whether the source of the iron is environmental or bacterially derived. E. coli bacterial cultures were grown in minimal media spiked with 10 \u00b5M 57FeSO4 then infected with phage T4 or T5. The phages were purified by methods of centrifugation, filtration, density-dependent ultracentrifugation, and dialyzing. The resulting phage fractions were quantified by SYBR epifluorescence microscopy and metal concentrations were measured on an ELEMENT XR ICP-MS.
attribute	NC_GLOBAL	title	String	[Fe-Labeling Expt: E.coli and phage T4 or T5] - Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019. (EAGER: Iron-Virus Interactions in the Ocean)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	date_utc		String
attribute	date_utc	bcodmo_name	String	date_utc
attribute	date_utc	description	String	UTC date when media was filtered and the experiment initiated
attribute	date_utc	long_name	String	Date Utc
attribute	date_utc	source_name	String	date_utc
attribute	date_utc	time_precision	String	1970-01-01
attribute	date_utc	units	String	unitless
variable	expt_round		float
attribute	expt_round	_FillValue	float	NaN
attribute	expt_round	actual_range	float	1.0, 5.0
attribute	expt_round	bcodmo_name	String	exp_id
attribute	expt_round	description	String	round of experiment and sample analysis on the XR ICP-MS
attribute	expt_round	long_name	String	Expt Round
attribute	expt_round	units	String	unitless
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	sample identifier: 1 for Fe-less (un-spiked) 0.02 um filtered M9 Minimal Media; 2 for 10 uM 57FeSO4 spiked 0.02 um filtered M9 minimal media; or 3 for 0.02 um filtered SM Buffer. Each stage of the experiment was designated sequentially as follows: 20’s for pelleted E. coli cultures grown to mid-logarithmic phase and rinsed three times in Fe-less media (no 57Fe spike) by centrifugation and re-suspension; 30’s for supernatant of pelleted bacteria (20’s); 40’s for unfiltered centrifuged supernatant of bacterial culture infected with phage overnight; 50’s for 0.22 um filtered dissolved fraction; 60’s for 0.02 um filtered soluble fraction; 70’s for bacterial samples treated with chloroform; 80’s for the supernatant (sample layer) above the sucrose cushion following ultracentrifugation; 90’s for sucrose cushion pellet re-suspended in 0.02 um filtered SM buffer; DIA for dialyzed samples post-dialysis over 6 buffer tank changes; and B for dialysis buffer: B1 pre-dialysis; B1-2 post-initial dialysis; B3-2 post-third dialysis buffer tank change; and B6-2 post- final dialysis buffer tank change. Samples were treated in triplicate: A; B; C for E. coli phage T4 samples; D; E; F for chloroform-lysed bacterial control samples; and G; H; I for Blanks; and M; N; O for E. coli phage T5 samples.
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	description		String
attribute	description	bcodmo_name	String	brief_desc
attribute	description	description	String	description of phage purification step and type of sample
attribute	description	long_name	String	Description
attribute	description	units	String	unitless
variable	Fe_56_nM		String
attribute	Fe_56_nM	bcodmo_name	String	Fe
attribute	Fe_56_nM	description	String	Concentration of 56Fe as determined by XR ICP-MS
attribute	Fe_56_nM	long_name	String	Fe 56 N M
attribute	Fe_56_nM	units	String	nanoMolar
variable	Fe_57_nM		String
attribute	Fe_57_nM	bcodmo_name	String	Fe
attribute	Fe_57_nM	description	String	Concentration of 57Fe as determined by XR ICP-MS
attribute	Fe_57_nM	long_name	String	Fe 57 N M
attribute	Fe_57_nM	units	String	nanoMolar
variable	volume		String
attribute	volume	bcodmo_name	String	volume
attribute	volume	description	String	sample volume
attribute	volume	long_name	String	Volume
attribute	volume	units	String	liters
variable	Fe_56_nmol		String
attribute	Fe_56_nmol	bcodmo_name	String	Fe
attribute	Fe_56_nmol	description	String	56Fe concentration as determined by XR ICP-MS
attribute	Fe_56_nmol	long_name	String	Fe 56 Nmol
attribute	Fe_56_nmol	units	String	nanomoles
variable	Fe_57_nmol		String
attribute	Fe_57_nmol	bcodmo_name	String	Fe
attribute	Fe_57_nmol	description	String	57Fe concentration as determined by XR ICP-MS
attribute	Fe_57_nmol	long_name	String	Fe 57 Nmol
attribute	Fe_57_nmol	units	String	nanomoles
variable	Cu_63_nmol		float
attribute	Cu_63_nmol	_FillValue	float	NaN
attribute	Cu_63_nmol	actual_range	float	6.03E-4, 265.0
attribute	Cu_63_nmol	bcodmo_name	String	Cu
attribute	Cu_63_nmol	description	String	63Cu concentration as determined by XR ICP-MS
attribute	Cu_63_nmol	long_name	String	Cu 63 Nmol
attribute	Cu_63_nmol	units	String	nanomoles
variable	Zn_66_nmol		float
attribute	Zn_66_nmol	_FillValue	float	NaN
attribute	Zn_66_nmol	actual_range	float	0.0, 13800.0
attribute	Zn_66_nmol	bcodmo_name	String	Zn
attribute	Zn_66_nmol	description	String	66Zn concentration as determined by XR ICP-MS
attribute	Zn_66_nmol	long_name	String	Zn 66 Nmol
attribute	Zn_66_nmol	units	String	nanomoles
variable	Ni_60_nmol		float
attribute	Ni_60_nmol	_FillValue	float	NaN
attribute	Ni_60_nmol	actual_range	float	2.19E-4, 1820.0
attribute	Ni_60_nmol	bcodmo_name	String	trace_metal_conc
attribute	Ni_60_nmol	description	String	60Ni concentration as determined by XR ICP-MS
attribute	Ni_60_nmol	long_name	String	Ni 60 Nmol
attribute	Ni_60_nmol	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Ni_60_nmol	units	String	nanomoles
variable	Pb_208_nmol		float
attribute	Pb_208_nmol	_FillValue	float	NaN
attribute	Pb_208_nmol	actual_range	float	5.05E-5, 340.0
attribute	Pb_208_nmol	bcodmo_name	String	trace_metal_conc
attribute	Pb_208_nmol	description	String	208Pb concentration as determined by XR ICP-MS
attribute	Pb_208_nmol	long_name	String	Pb 208 Nmol
attribute	Pb_208_nmol	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Pb_208_nmol	units	String	nanomoles
variable	bact_cells_ml		float
attribute	bact_cells_ml	_FillValue	float	NaN
attribute	bact_cells_ml	actual_range	float	0.344, 4.55E8
attribute	bact_cells_ml	bcodmo_name	String	bact_abundance
attribute	bact_cells_ml	description	String	SYBR epifluorescence bacterial counts
attribute	bact_cells_ml	long_name	String	Bact Cells Ml
attribute	bact_cells_ml	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bact_cells_ml	units	String	cells/milliliter
variable	phage_VPL_ml		double
attribute	phage_VPL_ml	_FillValue	double	NaN
attribute	phage_VPL_ml	actual_range	double	4.49E9, 4.62E11
attribute	phage_VPL_ml	bcodmo_name	String	cell_concentration
attribute	phage_VPL_ml	description	String	phage cell concentration
attribute	phage_VPL_ml	long_name	String	Phage VPL Ml
attribute	phage_VPL_ml	units	String	virus-like particles/milliliter
variable	Fe_57_atoms_per_phage		float
attribute	Fe_57_atoms_per_phage	_FillValue	float	NaN
attribute	Fe_57_atoms_per_phage	actual_range	float	74.3, 3200.0
attribute	Fe_57_atoms_per_phage	bcodmo_name	String	Fe
attribute	Fe_57_atoms_per_phage	description	String	57Fe content measured by XR ICP- MS; converted to moles; multiplied by Avogadro’s constant (6.022 x 1023 atoms/mol); and divided by number of phage in the sample
attribute	Fe_57_atoms_per_phage	long_name	String	Fe 57 Atoms Per Phage
attribute	Fe_57_atoms_per_phage	units	String	atoms
variable	notes_expt		String
attribute	notes_expt	bcodmo_name	String	comment
attribute	notes_expt	description	String	comments about samples
attribute	notes_expt	long_name	String	Notes Expt
attribute	notes_expt	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.