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Title Sum-
Institution Dataset ID
   set  data        files  public Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores
collected on R/V Thomas G. Thompson cruise TN309, May 2014
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Push cores (6.35 cm diameter) of sediment were collected in situ using the
manipulator arm of the Hybrid Remotely Operated Vehicle Nereus on the margin
of the Kermadec Trench (6013m) and along the main trench axis (7137 to 9177m)
at roughly 1000m increments.

Cores were sectioned at 1cm intervals and sieved for the meiofaunal fraction.

Upon retrieval of sediment cores, the 0-1, 1-2, 2-3, 3-5, 5-10 cm depth
horizons were sectioned and stored frozen (-80 \u00b0C) until analysis.
Benthic bacterial biomass were calculated from sediment concentrations of the
bacterial biomarker phospholipid fatty acids (PLFAs) i14:0, i15:0, ai15:0,
i16:0. In brief, PLFAs were extracted and purified from known quantities of
freeze-dried sediment samples and subsequently derivatized to yield fatty acid
methyl esters (FAMEs). FAMEs were quantified using GC-FID (Agilent
Technologies 6890N). Bacterial biomass was calculated using the well-
established PLFA:C conversion factor of 0.056 gC PLFA/gC biomass.

Attempts were made to obtain 3 replicate cores at each depth horizon (every
1000 meters from 6000m to 10,000m.\u00a0 For logistical reasons with vehicle
performance (and loss at 10,000), all of the replicates were not able to be
attribute NC_GLOBAL awards_0_award_nid String 536451
attribute NC_GLOBAL awards_0_award_number String OCE-1131620
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1131620 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Sediment sample log from push cores used for PLFA (phospholipid fatty acid) and OM (Organic Matter) analyses
collected at the Kermadec Trench on R/V Thomas G. Thompson cruise TN309, May 2014
PI: T. Shank (WHOI)
version: 2019-03-28
'n.u.' indicates deeper sections in the core that were not used for the analysis
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-03-28T13:17:19Z
attribute NC_GLOBAL date_modified String 2019-06-17T17:28:04Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.763873.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/763873 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String HROV Nereus
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 763881
attribute NC_GLOBAL instruments_0_description String Nereus is an efficient, multi-purpose “hybrid” vehicle that can explore and operate in the crushing pressures of the greatest ocean depths. An unmanned vehicle, Nereus operates in two complementary modes. It can swim freely as an autonomous underwater vehicle (AUV) to survey large areas of the depths, map the seafloor, and give scientists a broad overview. When Nereus locates something interesting, the vehicle’s support team can bring the vehicle back on board the ship and transforms it into a remotely operated vehicle (ROV) tethered to the ship via a micro-thin, fiber-optic cable. Through this tether, Nereus can transmit high-quality, real-time video images and receive commands from skilled pilots on the ship to collect samples or conduct experiments with a manipulator arm.

Technical specifications:

Weight on land: 2,800 kg
Payload capacity: 25 kg
Maximum speed: 3 knots
Batteries: rechargable lithium ion, 15 kilowatt hours in two pressure housings
Thrusters: 2 fore and aft, 2 vertical, 1 lateral (ROV mode) 2 fore and aft, 1 vertical (AUV mode)
Lights: variable output LED array, strobes
Manipulator arm: Kraft TeleRobotics 7-function hydraulic manipulator
Sonar: scanning sonar, forward look and profile, 675 KHz
Sensors: magnetometer, CTD (to measure conductivity, temperature, and depth)

Nereus supports a variety of science operations: Push coring, measuring heat flow, geotechnical and geochemical sensing, rock sampling and drilling, biological sampling, water sampling, high resolution acoustic bathymetry, and optical still and video imagery.

More information is available from the operator site at URL.
attribute NC_GLOBAL instruments_0_instrument_name String HROV Nereus
attribute NC_GLOBAL instruments_0_instrument_nid String 706
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 763892
attribute NC_GLOBAL instruments_1_description String Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB01/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Elemental Analyzer
attribute NC_GLOBAL instruments_1_instrument_nid String 546339
attribute NC_GLOBAL instruments_1_supplied_name String Fisons NA 1500 elemental analyzer
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Push cores were borrowed from the National Deep Submergence Facility to be able to standardize results. The push cores were 6.35cm diameter).
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 763882
attribute NC_GLOBAL instruments_2_description String Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.

Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
attribute NC_GLOBAL instruments_2_instrument_name String Push Corer
attribute NC_GLOBAL instruments_2_instrument_nid String 628287
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, core, cruise, cruise_id, data, dataset, date, dive, dmo, erddap, horizon, horizon_core_cm, management, NiWa_id, oceanography, office, preliminary, sample, site, station, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/763873/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/763873 (external link)
attribute NC_GLOBAL param_mapping String {'763873': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/763873/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_0_affiliation_acronym String WHOI
attribute NC_GLOBAL people_0_person_name String Timothy M. Shank
attribute NC_GLOBAL people_0_person_nid String 536454
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Hawaii at Manoa
attribute NC_GLOBAL people_1_affiliation_acronym String SOEST
attribute NC_GLOBAL people_1_person_name String Jeffrey C. Drazen
attribute NC_GLOBAL people_1_person_nid String 491313
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Whitman College
attribute NC_GLOBAL people_2_person_name String Paul Yancey
attribute NC_GLOBAL people_2_person_nid String 536477
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String HADES
attribute NC_GLOBAL projects_0_acronym String HADES
attribute NC_GLOBAL projects_0_description String Extracted from the NSF award abstract:
Severe technical challenges associated with the extremes of hydrostatic pressure have prevented major advances in hadal ecological studies, and relegated hadal systems to among the most poorly investigated habitats on Earth. Through this project, Hadal Ecosystems Studies (HADES) program, PIs will determine the composition and distribution of hadal species, the role of hadal pressures (piezolyte concentrations, enzyme function under pressure), food supply (distribution of POC with the abundance and biomass of trench organisms, and metabolic rates/energetic demand), and depth/topography (genetic divergence and spatial connectivity of populations) have on impacting deep-ocean community structure. This project will examine these factors using the world's first full-ocean depth hybrid remotely operated vehicle (HROV) in conjunction with the only full-ocean depth imaging lander (Hadal-Lander). This project will provide the first seafloor data and samples in one of the world's best, yet little known trenches- the Kermadec Trench (SW Pacific Ocean).  Megafaunal community structure and the relationship between POC and benthic bacterial biomass will be examined as a function of depth and location by systematic high-definition imaging and sediment/faunal sampling transects from abyssal to full trench depths both along and perpendicular to the trench axis. Population genetic approaches will provide levels of genetic divergence and evolutionarily independent lineages to assess the role of depth and topography in trenches and their adjacent abyssal plain in promoting the formation of species. Physiological constraints will be investigated by examining in-situ respiration of selected fauna and tissue concentrations of such protein stabilizers as trimethylamine oxide (TMAO), and the structural adaptations of macromolecules. 
Image of NEREUS Deployment Sites. [click on the image to view a larger version]
attribute NC_GLOBAL projects_0_end_date String 2015-08
attribute NC_GLOBAL projects_0_geolocation String Kermadec Trench adjacent to New Zealand: approximately 37 12.75 S and 178 51.43 E to 31 51.29 S and 176 49.07 W
attribute NC_GLOBAL projects_0_name String Controls on Hadal Megafaunal Community Structure: a Systematic Examination of Pressure, Food Supply, and Topography
attribute NC_GLOBAL projects_0_project_nid String 536452
attribute NC_GLOBAL projects_0_project_website String https://www.whoi.edu/hades/ (external link)
attribute NC_GLOBAL projects_0_start_date String 2011-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String cruise_id
attribute NC_GLOBAL summary String This dataset contains the sample log for HADESK bacterial biomarker phospholipid fatty acid (PLFA) content from sediment push cores. The same samples were also used for the organic matter (OM) analysis. Samples were taken in the Kermadec Trench in the Southwest Pacific, 4000 to ~10,000m from the RV/ Thomas G. Thompson during cruise TN309, May 2014.
attribute NC_GLOBAL title String Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable sample   byte  
attribute sample _FillValue byte 127
attribute sample actual_range byte 1, 81
attribute sample bcodmo_name String sample
attribute sample description String sample identifier
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable cruise_id   String  
attribute cruise_id bcodmo_name String cruise_id
attribute cruise_id description String cruise identifier
attribute cruise_id long_name String Cruise Id
attribute cruise_id units String unitless
variable dive   String  
attribute dive bcodmo_name String dive_id
attribute dive description String dive identifier
attribute dive long_name String Dive
attribute dive units String unitless
variable site   String  
attribute site bcodmo_name String site
attribute site description String site name
attribute site long_name String Site
attribute site units String unitless
variable date_utc   String  
attribute date_utc bcodmo_name String date_utc
attribute date_utc description String sample collection date in UTC
attribute date_utc long_name String Date Utc
attribute date_utc source_name String date_utc
attribute date_utc time_precision String 1970-01-01
attribute date_utc units String unitless
variable station   String  
attribute station bcodmo_name String station
attribute station description String station identifier
attribute station long_name String Station
attribute station units String unitless
variable core   String  
attribute core bcodmo_name String core_id
attribute core description String core identifier
attribute core long_name String Core
attribute core units String unitless
variable NiWa_id   String  
attribute NiWa_id bcodmo_name String sample
attribute NiWa_id description String National Institute for Water and Atmosphere identifier
attribute NiWa_id long_name String Ni Wa Id
attribute NiWa_id nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute NiWa_id units String unitless
variable horizon_core_cm   String  
attribute horizon_core_cm bcodmo_name String depth_core
attribute horizon_core_cm description String depth horizon in core
attribute horizon_core_cm long_name String Horizon Core Cm
attribute horizon_core_cm units String centimeters

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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