ERDDAP > info > bcodmo_dataset_764480

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Fe, Pu partitioning and organic biopolymers] - Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)		I   M	background			BCO-DMO	bcodmo_dataset_764480

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	The seawater (< 1 kDa) was enriched with f/2 nutrients, trace metals and vitamins, and autoclaved in pre-combusted and seawater-preconditioned clear glassware. Known activity of 59Fe (gamma emitting radionuclide) and 238Pu (alpha emitting radionuclide) were added into the seawater in pre-combusted and seawater-preconditioned clear glassware.\u00a0 After checking the pH of each radiolabeled medium to be 8.0, laboratory axenic Skeletonema costatum (UTEX LB 2308) and Emiliania huxleyi (CCMP 371) was added to 100 mL of media and incubated at a temperature of 19\u00b11\u00baC with a light:dark cycle of 14 h:10 h under an irradiation condition of 100 \u00b5mol-quanta/m2/s. The sequential chemical extraction scheme for obtaining individual fractions from S. costatum and E. huxleyi followed the procedures described in Chuang et al. (2015) and Lin et al. (2017), with a few exceptions. For the extracellular biopolymers excreted by the phytoplankton, non-attached exopolymeric substances (NAEPS) in the surrounding seawater and attached EPS (AEPS) associated with cellular surface, were harvested. Laboratory cultures were centrifuged at 3000 x g for 30 min, followed by filtration of the supernatant which was further concentrated and desalted with nanopure water (18.2 \u03a9) in 3 kDa Microsep centrifugal filter tubes (Milipore) to obtain the NAEPS fraction, while the resultant pellet from the centrifugation was resuspended by 50 mL 3% NaCl solution and stirred gently overnight at 4\u00baC to extract EPS from the cellular surface. The solution was also centrifuged, and the supernatant containing the AEPS was then filtered to remove residual cells before further desalting via the 3 kDa ultrafiltration centrifugation tubes. The final volume of concentrated solution of each biopolymer fraction (>3 kDa) was 2 mL. For the S. costatum cultures, 10 mL of 100 mM EDTA (pH 8.0) solution was added to the diatom cells from the previous AEPS extraction step. The diatom cells were resuspended at 4\u00baC overnight to extract the intracellular material after diatom cell lysis and the supernatant was collected after centrifugation to obtain the EDTA-extractable intracellular biopolymers. Then, the resultant pellet was further resuspended in 10 mL of 1% SDS/10 mM Tris (pH 6.8) solution and heated at 95\u00baC for 1 hr. The centrifuged supernatant was also collected and defined as SDS-extractable biopolymer in S. costatum cells.\u00a0 To access the diatom frustule-associated biopolymers, 5 mL of 52% HF was then added to the frustules and incubated on ice for 1 hr. After the separation of HF-insoluble pellet, the HF-soluble fraction was evaporated under N2 stream and neutralized, followed by the 3 kDa centrifugal filtration to collect the digested frustule silica fraction (<3 kDa) and HF-soluble frustule-associated biopolymer (>3 kDa). Lastly, the residue biopolymer in the HF-insoluble pellet was collected with the resuspension in a 2 mL of 100 mM ammonium acetate solution and sonication. Similar to NAEPS and AEPS, all the S. costatum cellular biopolymers were concentrated and desalted with nanopure water in 3 kDa Microsep centrifugal filter tubes (Milipore). The coccosphere of the E. huxleyi cells was first dissolved before the extraction of intracellular biopolymers. In brief, the pellet from the previous AEPS extraction step was digested in 0.44 M acetic acid (HAc) (weak acidity and non-oxidizing nature to avoid the breakage of cells) plus 0.1 M NaCl solution at 4\u00baC for 8 hr. After the digestion, the mixed solution was centrifuged and filtered, followed by ultrafiltration of the supernatant with 3 kDa Microsep centrifugal filter tubes. The retentate (>3 kDa) was defined as coccosphere-associated biopolymers, and the permeate fraction (<3 kDa) was also collected to obtain the fraction of digested biogenic calcite. The E. huxleyi cells after the removal of shells were further heated in 20 mL of 1% SDS/10 mM Tris mixed solution (pH 6.8) at 95 \u00baC for 1 hr. The supernatant was also collected through centrifugation and filtration, followed by desalting with 3 kDa Microsep centrifugal filter tubes. Subsequently, the remaining pellet was further digested by 0.04 M NH2OH\u2022HCl/4.35 M HAc mixture at 96 \u00baC for 6 hr to obtain the intracellular metabolitic biopolymer. The sum of these two fractions represents the intracellular biopolymers in E. huxleyi cells. All the solutions from the different extraction steps, including the >3 kDa biopolymer fractions and the permeate (< 3 kDa, i.e., frustule and coccosphere), were counted to determine the activity of 59Fe and 238Pu. 59Fe activity was directly obtained from a Canberra ultrahigh purity germanium well gamma detector at the decay energies of 1099 kev. All the solutions for the gamma counting had the same volume and geometry to avoid geometry corrections, and all the data were decay corrected.\u00a0 238Pu activities were determined by alpha-spectroscopy (Xu et al., 2016). Briefly, a known activity of 242Pu was spiked to trace the yield of 238Pu during the extraction steps. The samples were oven-dried, then heated at 600 \u00baC overnight in a ceramic crucible. The resulting ash fraction was then digested in Teflon tubes overnight in concentrated HNO3 and HCl (1:1) at 85\u00baC. The remaining solid residual fraction was collected by centrifugation and discarded, and the supernatant was further evaporated to incipient dryness. To convert all Pu ions to Pu(IV), a FeSO4\u20227H2O (0.2 g/mL) solution, followed by 0.25 g of NaNO2, were added to each sample to achieve a final volume of 3 mL for each sample. Samples were then passed through an UTEVA column (Cat. # UT-C50-A, Eichrom, USA) to separate Pu from other alpha-emitting radionuclides (e.g., 238U, 241Am). After washing the column with an 8 M HNO3 solution, the Pu was eluted using freshly-prepared 0.02 M NH2OH\u2022HCl/0.02 M ascorbic acid in 2 M HNO3. The Pu-containing eluent was evaporated and re-constituted in 0.4 M (NH4)2SO4 (pH~2.6) for electroplating onto a stainless steel planchet at 0.6 Amps current for 2 hr. Sample-bearing planchets were then analyzed via alpha spectroscopy for at least one week to obtain counting errors (1 sigma) lower than 5%. Subsamples were taken from the concentrated biopolymers for the analysis of protein, total carbohydrate (TCHO) and uronic acid (URA), respectively. In brief, the protein abundance was measured through a modified Lowry protein assay, using bovine serum albumin (BSA) as the standard. For the concentrations of TCHO, samples were hydrolyzed by 0.09 M HCl (final concentration) at 150\u00baC for 1 h. After neutralization with NaOH solution, the hydrolysate was measured by the 2,4,6-tripyridyl-triazine (TPTZ) method (Hung et al., 2001), with glucose as the standard. URA concentrations were determined by the metahydroxyphenyl method using glucuronic acid as the standard (Hung and Santschi, 2001).\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	735995
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1356453
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1356453
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum PI: Peter H. Santschi Version: 2019-04-08
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-04-08T17:02:21Z
attribute	NC_GLOBAL	date_modified	String	2019-04-08T19:26:10Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.764480.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/764480
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Spectrometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Sample-bearing planchets were then analyzed via alpha spectroscopy for at least one week to obtain counting errors (1 sigma) lower than 5%.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	764489
attribute	NC_GLOBAL	instruments_0_description	String	A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Spectrometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	667
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Canberra Quad Alpha Spectrometer Model 7404
attribute	NC_GLOBAL	instruments_1_acronym	String	Spectrometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	UV-Visible spectrometer, BioTek Instruments Inc Model EPOCH
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	764490
attribute	NC_GLOBAL	instruments_1_description	String	A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Spectrometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	667
attribute	NC_GLOBAL	instruments_1_supplied_name	String	UV-Visible spectrometer, BioTek Instruments Inc Model EPOCH
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Beckman Coulter Allegra X-12 centrifuge
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	764491
attribute	NC_GLOBAL	instruments_2_description	String	A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Centrifuge
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	629890
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Beckman Coulter Allegra X-12 centrifuge
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Canberra ultrahigh purity germanium well gamma detector Model GCW3024
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	764492
attribute	NC_GLOBAL	instruments_3_description	String	Instruments measuring the relative levels of electromagnetic radiation of different wavelengths in the gamma-ray waveband.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Gamma Ray Spectrometer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	670659
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Canberra ultrahigh purity germanium well gamma detector Model GCW3024
attribute	NC_GLOBAL	keywords	String	act, bco, bco-dmo, biological, biopolymer, Biopolymer_fraction, cell, Cell_type, chemical, data, dataset, dmo, erddap, fe59, Fe59_act_pcnt, fraction, management, oceanography, office, pcnt, pcnt_URA_TCHO, preliminary, protein, Protein_C_TCHO_C, pu238, Pu238_act_pcnt, tcho, type, ura
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/764480/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/764480
attribute	NC_GLOBAL	param_mapping	String	{'764480': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/764480/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_0_person_name	String	Peter Santschi
attribute	NC_GLOBAL	people_0_person_nid	String	735998
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_1_person_name	String	Antonietta Quigg
attribute	NC_GLOBAL	people_1_person_nid	String	736000
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_2_person_name	String	Kathleen Schwehr
attribute	NC_GLOBAL	people_2_person_nid	String	736002
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_3_person_name	String	Chen Xu
attribute	NC_GLOBAL	people_3_person_nid	String	736004
attribute	NC_GLOBAL	people_3_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_3_role_type	String	originator
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Mathew Biddle
attribute	NC_GLOBAL	people_4_person_nid	String	708682
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_acronym	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Particle-associated natural radioisotopes are transported to the ocean floor mostly via silica and carbonate ballasted particles, allowing their use as tracers for particle transport. Th(IV), Pa (IV,V), Po(IV), Pb(II) and Be(II) radionuclides are important proxies in oceanographic investigations, used for tracing particle and colloid cycling, estimating export fluxes of particulate organic carbon, tracing air-sea exchange, paleoproductivity, and/or ocean circulation in paleoceanographic studies. Even though tracer approaches are considered routine, there are cases where data interpretation or validity has become controversial, largely due to uncertainties about inorganic proxies and organic carrier molecules. Recent studies showed that cleaned diatom frustules and pure silica particles, sorb natural radionuclides to a much lower extent (by 1-2 orders of magnitude) than whole diatom cells (with or without shells). Phytoplankton that build siliceous or calcareous shells, such as the diatoms and coccolithophores, are assembled via bio-mineralization processes using biopolymers as nanoscale templates. These templates could serve as possible carriers for radionuclides and stable metals. In this project, a research team at the Texas A & M University at Galveston hypothesize that radionuclide sorption is controlled by selective biopolymers that are associated with biogenic opal (diatoms), CaCO3 (coccolithophores) and the attached exopolymeric substances (EPS), rather than to pure mineral phase. To pursue this idea, the major objectives of their research will include separation, identification and molecular-level characterization of the individual biopolymers (e.g., polysaccharides, uronic acids, proteins, hydroquinones, hydroxamate siderophores, etc.) that are responsible for binding different radionuclides (Th, Pa, Pb, Po and Be) attached to cells or in the matrix of biogenic opal or CaCO3 as well as attached EPS mixture, in laboratory grown diatom and coccolithophore cultures. Laboratory-scale radiolabeling experiments will be conducted, and different separation techniques and characterization techniques will be applied. Intellectual Merit : It is expected that this study will help elucidate the molecular basis of the templated growth of diatoms and coccoliths, EPS and their role in scavenging natural radionuclides in the ocean, and help resolve debates on the oceanographic tracer applications of different natural radioisotopes (230,234Th, 231Pa, 210Po, 210Pb and 7,10Be). The proposed interdisciplinary research project will require instrumental approaches for molecular-level characterization of these radionuclides associated carrier molecules. Broader Impacts: The results of this study will be relevant for understanding biologically mediated ocean scavenging of radionuclides by diatoms and coccoliths which is important for carbon cycling in the ocean, and will contribute to improved interpretation of data obtained by field studies especially through the GEOTRACES program. This new program will enhance training programs at TAMUG for postdocs, graduate and undergraduate students. Lastly, results will be integrated in college courses and out-reach activities at Texas A&M University, including NSF-REU, Sea Camp, Elder Hostel and exhibits at the local science fair and interaction with its after-school program engaging Grade 9-12 students from groups traditionally underrepresented.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-02
attribute	NC_GLOBAL	projects_0_name	String	Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores
attribute	NC_GLOBAL	projects_0_project_nid	String	735996
attribute	NC_GLOBAL	projects_0_start_date	String	2014-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Iron (Fe), a micronutrient for algal growth, and plutonium (Pu), an anthropogenic radionuclide, share some common features. This includes similar oceanic distributions when different input modes are taken into account, as well as their chemical behavior, such as a high affinity to natural organic matter (NOM). The NOM produced by various phytoplankton communities can potentially influence Fe cycling in the ocean, and likely also influence the transport behavior of Pu. We conducted laboratory incubation experiments using the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum, in the presence of 59Fe and 238Pu as radiotracers, in order to differentiate Fe and Pu uptake by extracellular exopolymeric substances (EPS) and intracellular biopolymers. The Fe and Pu distributions in select organic compound classes including proteins, total carbohydrates (TCHO) and uronic acids (URA) produced by these two types of phytoplankton were compared. Our results indicated that most of the Fe and Pu (>95%) were found concurrently concentrated in E. huxleyi-derived non-attached EPS, while much less (<2%) was present in the intracellular fraction of E. huxleyi. By contrast, in the diatom S. costatum, both Fe and Pu distribution was EPS > intracellular biopolymers > outer cell covering (i.e., frustule). In fact, over 50% of Fe was concentrated in S. costatum-derived attached EPS and intracellular biopolymers. The diatom derived Fe-EPS complexes were more hydrophobic, with stronger tendency to aggregate in seawater. Fe binding to biopolymers in both E. huxleyi and S. costatum cultures was related to URA concentrations, but the overall distribution of URA between these two phytoplankton species was different. Our findings suggest that the presence of URA in S. costatum cellular surface (i.e., attached EPS) and its intracellular fraction could be an indicator for the Fe transport from the surrounding seawater to the diatom cells. However, for the coccolithophore E. huxleyi, Fe appeared not to be efficiently taken up during its growth. Instead, the more hydrophilic non-attached EPS (i.e., low protein/TCHO ratio) produced by E. huxleyi could have stabilized Fe in the colloidal form as Fe-EPS complexes. Similar partitioning behavior of Fe and Pu suggests that Pu isotopes can potentially serve as a tracer for the Fe biogeochemistry in the ocean.
attribute	NC_GLOBAL	title	String	[Fe, Pu partitioning and organic biopolymers] - Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	type		String
attribute	type	bcodmo_name	String	sample_descrip
attribute	type	description	String	type
attribute	type	long_name	String	Type
attribute	type	units	String	unitless
variable	Biopolymer_fraction		String
attribute	Biopolymer_fraction	bcodmo_name	String	sample_descrip
attribute	Biopolymer_fraction	description	String	Biopolymer fraction type
attribute	Biopolymer_fraction	long_name	String	Biopolymer Fraction
attribute	Biopolymer_fraction	units	String	unitless
variable	Cell_type		String
attribute	Cell_type	bcodmo_name	String	sample_descrip
attribute	Cell_type	description	String	cell type
attribute	Cell_type	long_name	String	Cell Type
attribute	Cell_type	units	String	unitless
variable	Fe59_act_pcnt		String
attribute	Fe59_act_pcnt	bcodmo_name	String	unknown
attribute	Fe59_act_pcnt	description	String	Activity percentage
attribute	Fe59_act_pcnt	long_name	String	Fe59 Act Pcnt
attribute	Fe59_act_pcnt	units	String	unitless (%)
variable	Pu238_act_pcnt		String
attribute	Pu238_act_pcnt	bcodmo_name	String	unknown
attribute	Pu238_act_pcnt	description	String	Activity percentage
attribute	Pu238_act_pcnt	long_name	String	Pu238 Act Pcnt
attribute	Pu238_act_pcnt	units	String	unitless (%)
variable	Protein		String
attribute	Protein	bcodmo_name	String	unknown
attribute	Protein	description	String	amount of protein
attribute	Protein	long_name	String	Protein
attribute	Protein	units	String	microMole Carbon (uM-C)
variable	TCHO		float
attribute	TCHO	_FillValue	float	NaN
attribute	TCHO	actual_range	float	1.0, 38.2
attribute	TCHO	bcodmo_name	String	unknown
attribute	TCHO	description	String	amount of TCHO-total carbohydrate
attribute	TCHO	long_name	String	TCHO
attribute	TCHO	units	String	microMole Carbon (uM-C)
variable	URA		float
attribute	URA	_FillValue	float	NaN
attribute	URA	actual_range	float	0.2, 38.2
attribute	URA	bcodmo_name	String	unknown
attribute	URA	description	String	amount of URA-uronic acid
attribute	URA	long_name	String	URA
attribute	URA	units	String	microMole Carbon (uM-C)
variable	Protein_C_TCHO_C		float
attribute	Protein_C_TCHO_C	_FillValue	float	NaN
attribute	Protein_C_TCHO_C	actual_range	float	0.0, 7.8
attribute	Protein_C_TCHO_C	bcodmo_name	String	unknown
attribute	Protein_C_TCHO_C	description	String	amount of protein to total carbohydrates
attribute	Protein_C_TCHO_C	long_name	String	Protein C TCHO C
attribute	Protein_C_TCHO_C	units	String	microMole Carbon (uM-C)
variable	pcnt_URA_TCHO		byte
attribute	pcnt_URA_TCHO	_FillValue	byte	127
attribute	pcnt_URA_TCHO	actual_range	byte	13, 100
attribute	pcnt_URA_TCHO	bcodmo_name	String	unknown
attribute	pcnt_URA_TCHO	description	String	percent uronic acid to total carbohydrates
attribute	pcnt_URA_TCHO	long_name	String	Pcnt URA TCHO
attribute	pcnt_URA_TCHO	units	String	microMole Carbon (uM-C)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.