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Title Sum-
Institution Dataset ID
   set  data   graph     files  public Coastal water biogeochemistry collected aboard the R/V Endeavor along the North Atlantic
coast from 2017-08-20 to 2017-08-28
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Chlorophyll
In the dark, 250 mL of seawater was filtered onto 25 mm GF/F filters.\u00a0
Samples were stored in the dark at -20C until analyzed according to protocols
adapted from Strickland and Parsons (1972).\u00a0 Briefly, samples were
extracted in 90% acetone in the dark (4C, 9 hr) and measured using an 10AU
fluorometer (Turner).\u00a0 Sample signals were calibrated using a
chlorophyll-a standard (Sigma C6144) and were corrected for pheopigments by
accounting for the fluorescence of extracts before and after acidification to
0.003 M HCl.

Nitrogen Species
Concentrations of nitrate + nitrite were measured by chemiluminescence after
reduction in a hot acidic vanadyl sulfate solution on a NOx analyzer (Braman
and Hendrix, 1989). Concentrations of nitrite were quantified by using the
Griess\u2013Ilosvay method followed by measuring absorption 540 nm (Grasshoff
et al., 1999), and nitrate was quantified by difference. Concentrations of
ammonium were measured by fluorescence using the OPA method (Holmes et al.,

Microbial Abundance
Seawater samples were preserved for flow cytometry with 0.5% glutaraldehyde
(final concentration), flash frozen in liquid nitrogen and stored at
-80\u00b0C until analysis. Bacteria and group-specific phytoplankton counts
were conducted on a Guava EasyCyte HT flow cytometer (Millipore). Instrument-
specific beads were used to calibrate the cytometer. Samples were analyzed at
a low flow rate (0.24 \u00b5L s-1) for 3 min.\u00a0 To enumerate bacteria,
samples were diluted (1:100) with filtered seawater (0.01 \u00b5m).\u00a0
Samples and filtered seawater blanks were stained with SYBR Green I
(Invitrogen) according to the manufacturer\u2019s instructions and incubated
in a 96-well plate in the dark at room temperature for 1 hr.\u00a0 Bacterial
cells were counted based on diagnostic forward scatter vs. green fluorescence
signals.\u00a0 Major phytoplankton groups were distinguished based on plots of
forward scatter vs. orange (phycoerythrin-containing Synechococcus sp.), and
forward scatter vs. red (eukaryotes).\u00a0 Size classes of eukaryotic
phytoplankton were further distinguished based on forward scatter (pico-,
nano- and large eukaryotes).\u00a0

Dissolved organic carbon
Filtered water samples for total dissolved organic carbon were pipetted into
acid-washed combusted glass vials, acidified to pH = 2 with 12 M hydrochloric
acid, and stored at 4 \u00b0C until analysis on a Shimadzu TOC-5050A total
organic carbon analyzer. The coefficient of variability between replicate
injections was less than 1%.

Reactive oxygen species
Water samples were collected directly from a trace metal clean rosette into
acid-washed, opaque bottles and stored in a shipboard flow-through seawater
incubator. A subset of each water sample was filtered (0.2 um), amended with
50 \u00b5M diethylene-triaminepentaacetic acid (DTPA, Sigma), and aged
overnight in the shipboard seawater flow through incubator (termed AFSW).
Additionally, particle associated superoxide signals were determined by
filtering (0.2 um) a subset of each water sample and measuring the
chemiluminescent signal within 25-30 minutes after filtering (termed FFSW).
Superoxide signals were measured by pumping unfiltered water (UFSW), FFSW, or
AFSW from dark bottles using a high accuracy peristaltic pump directly into a
flow-through FeLume Mini system (Waterville Analytical, Waterville ME) within
the ship laboratory. Superoxide detection was based on the reaction between
superoxide and a chemiluminescent probe, a methyl cypridina luciferin analog
(MCLA, Santa Cruz Biotechnology) (Rose et al., 2008) as before (Roe et al.,
2016). The travel time of the water samples in the opaque FeLume tubing was
approximately 20 sec. Data was collected for several minutes (~2-4 min) once a
steady-state signal was achieved. At least 12 hours following filtering, the
superoxide signals within the AFSW for each depth was measured to establish
the baseline. At the end of each run, 800 U L-1 superoxide dismutase (SOD,
Sigma) was added to seawater samples. The total dark superoxide signal
produced in the seawater was defined as the difference between the UFSW signal
(subtracted by the SOD baseline) and AFSW signal (subtracted by the SOD
baseline) (similar to (Roe et al., 2016)). The particle associated signal was
defined as the UFSW signal (subtracted by the SOD baseline) and FFSW signal
(subtracted by the SOD baseline) (similar to (Roe et al., 2016)). While the
SOD baseline has an autoxidation artifact, this artifact is canceled by taking
the difference between two signals. The chemiluminescent signals were
converted to superoxide concentration by conducted calibrations in the same
aged-filtered seawater used for the baseline at each depth. Calibrations were
conducted using potassium dioxide (Sigma) as detailed previously (Zhang et
al., 2016).\u00a0

Designations are:\u00a0
Total superoxide = [(UFSW) - (UFSW-SOD)] - [(AFSW)- (AFSW-SOD)]
Particle superoxide = [(UFSW) - (UFSW-SOD)] - [(FFSW)- (FFSW-SOD)]

Hydrogen peroxide concentrations. Hydrogen peroxide concentration was
quantified based on the oxidation of colorless AmplifluTM Red (AR, Sigma) to
pink-colored resorufin by hydrogen peroxide. For hydrogen peroxide
concentration analysis, pre-mixed AR and horse radish peroxidase (HRP) stock
solution was added at a final concentration of 50 \u00b5mol L-1 AR and 1 kU
L-1 HRP to filtered (0.2 um) and unfiltered seawater samples in a clear
96-well microplate (Zhang et al., 2016). Light absorbance was measured at 570
nm (Abs570, maximum absorbance of resorufin) and 700 nm (Abs700, to account
for background absorbance) on a SpectraMax\u00ae M3 multi-mode microplate
reader. The difference between Abs570 and Abs700 (i.e., Abs570-700) was used
for calculating hydrogen peroxide concentrations in seawater samples based on
a calibration. The calibration factor was determined by standard addition of
hydrogen peroxide into 0.2-\u00b5m filtered seawater from each station and
depth as described previously (Zhang et al., 2016). To account for
autoxidation of AR, 200 kU L-1 catalase (Sigma) was added to the blanks prior
to the addition of AR and HRP. The hydrogen peroxide concentrations in
seawater samples were determined by applying the calibration factor to the
blank-corrected Abs570-700values. Net and gross hydrogen peroxide
concentrations were defined as the levels measured in unfiltered and filtered
seawater samples, respectively.\u00a0

Seawater samples (1 L) were filtered through 0.2 \u00b5m membrane filters
(Millipore) within one hour of collection using acid-washed Savillex vacuum-
filtration rigs. The filtrate was poured into new 15 mL falcon tubes and the
filter was immediately amended with a leuco-based dye for Mn oxide
concentration in a separate 15 mL falcon tube.

The leucoberbelin blue (LBB) assay for Mn oxides (denoted MnOx hereafter) was
previously adapted from Altman (1972) to examine coastal water column sites
(Oldham et al., 2015; 2017a, 2017b, 2017c). In this assay, the filter is
amended with 3 mL of 20 \u00b5M LBB dye solution (LBB, Sigma-Aldrich). The dye
color forms upon oxidation of the LBB molecule by Mn oxides and can be
measured spectrophotometrically. The LBB stock solution was prepared by
dissolving the powder in Milli-Q water to a concentration of 4 % and adding 40
\u00b5L of 10 M sodium hydroxide (NaOH) per 10 mL of stock solution. Working
solutions are subsequently prepared by diluting the stock solution into 1 %
acetic acid, to 0.4 % LBB. A calibration curve was generated using KMnO4, for
which equivalent absorbance for Mn(IV) is calculated based on 2.5 more Mn(IV)
being required relative to Mn(VII) to oxidize the LBB. In our set-up, a 100 cm
pathlength cell (Liquid Waveguide Capillary Cell) was coupled to a Flame UV-
Vis (Ocean Optics), set up with SpectraSuite software. Using a 100 cm
pathlength cell allows for a detection limit of 0.2 nM but also requires our
re-filtration of samples (0.2 \u00b5m luer-lok syringe filter, Millipore)
prior to injection into the cell to avoid particulate interference and
clogging of the capillary cell. Samples reacted with the LBB dye overnight in
the dark prior to analysis, then absorbance at 623 nm was recorded.\u00a0 If
sample absorbance was too high, samples were diluted 10-20 times in Milli-Q

For soluble Mn speciation analysis, an established spectrophotometric
porphyrin addition method was employed (Madison et al., 2011; Oldham et al.,
2017a), which uses the ligand T(4-CP)P (or \u03b1, \u03b2, \u03b3,
\u03b4-tetrakis(4-carboxyphenyl)porphine, to 2.33 x 10-7 M final sample
cocentration). In this method, cadmium chloride (CdCl2; to 2.4 x 10-7 M) is
added to form a complex with the porphyrin, in the presence of an imidazole
tetraborate buffer (pH = 8.2). The sample is added to the mixture (diluted
10-fold with Milli-Q water to avoid chloride interference) and any Mn(II) in
the sample undergoes a metal substitution reaction with the Cd over the course
of a 1 hour reaction in a 90\u00b0C water bath. The solution is cooled, then
analyzed using the 100-cm UV-Vis spectrophotometric set-up described above.
Total dissolved Mn is analyzed in the same way, but after the addition of 1.4
\u00b5M hydroxylamine hydrochloride to the sample (reacted overnight in a
refrigerator). The difference between the total dissolved Mn and the Mn(II)
gives the Mn(III)-L in the sample. We note that during the heated reaction
with no reducing agent, it is likely that some Mn(III)-L complexes undergo a
ligand substitution reaction with the added porphyrin, and thus our method
likely underestimates Mn(III)-L, particularly for weaker complexes. For all
samples, assays were run in triplicate for both Mn(II) and Mn total, and peak
height for all assays was determined using a baseline subtraction performed
using ECD-Soft peak correction software.
attribute NC_GLOBAL awards_0_award_nid String 756929
attribute NC_GLOBAL awards_0_award_number String OCE-1355720
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1355720 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Coastal water biogeochemistry along the North Atlantic coast
PI: Colleen Hansel
Version: 2019-04-24
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-04-18T19:47:26Z
attribute NC_GLOBAL date_modified String 2019-09-13T13:00:25Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.765327.1
attribute NC_GLOBAL Easternmost_Easting double -67.92692
attribute NC_GLOBAL geospatial_lat_max double 41.18026
attribute NC_GLOBAL geospatial_lat_min double 37.77692
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -67.92692
attribute NC_GLOBAL geospatial_lon_min double -74.56461
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 2031.208
attribute NC_GLOBAL geospatial_vertical_min double 2.016
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/765327 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String CTD Sea-Bird
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Samples were collected using a 12-bottle trace metal clean CTD (Conductivity, Temperature and Depth) rosette
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 765588
attribute NC_GLOBAL instruments_0_description String Conductivity, Temperature, Depth (CTD) sensor package from SeaBird Electronics, no specific unit identified. This instrument designation is used when specific make and model are not known. See also other SeaBird instruments listed under CTD. More information from Sea-Bird Electronics.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/130/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String CTD Sea-Bird
attribute NC_GLOBAL instruments_0_instrument_nid String 447
attribute NC_GLOBAL instruments_0_supplied_name String CTD Seabird
attribute NC_GLOBAL instruments_1_acronym String Fluorometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Briefly, samples were extracted in 90% acetone in the dark (4C, 9 hr) and measured using an 10AU fluorometer (Turner).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 765589
attribute NC_GLOBAL instruments_1_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_1_instrument_nid String 484
attribute NC_GLOBAL instruments_1_supplied_name String 10AU fluorometer (Turner)
attribute NC_GLOBAL instruments_2_acronym String TOC analyzer
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Filtered water samples for total dissolved organic carbon were pipetted into acid-washed combusted glass vials, acidified to pH = 2 with 12 M hydrochloric acid, and stored at 4 °C until analysis on a Shimadzu TOC-5050A total organic carbon analyzer.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 765592
attribute NC_GLOBAL instruments_2_description String A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB04/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Total Organic Carbon Analyzer
attribute NC_GLOBAL instruments_2_instrument_nid String 652
attribute NC_GLOBAL instruments_2_supplied_name String Shimadzu TOC-5050A total organic carbon analyzer
attribute NC_GLOBAL instruments_3_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Bacteria and group-specific phytoplankton counts were conducted on a Guava EasyCyte HT flow cytometer (Millipore).
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 765591
attribute NC_GLOBAL instruments_3_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_3_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_3_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_3_instrument_nid String 660
attribute NC_GLOBAL instruments_3_supplied_name String Guava EasyCyte HT flow cytometer (Millipore)
attribute NC_GLOBAL instruments_4_dataset_instrument_description String Concentrations of nitrate + nitrite were measured by chemiluminescence after reduction in a hot acidic vanadyl sulfate solution on a NOx analyzer (Braman and Hendrix, 1989)
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 765590
attribute NC_GLOBAL instruments_4_description String The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX.

One example is the Teledyne Model T200: http://www.teledyne-api.com/products/T200.asp
attribute NC_GLOBAL instruments_4_instrument_name String Chemiluminescence NOx Analyzer
attribute NC_GLOBAL instruments_4_instrument_nid String 542895
attribute NC_GLOBAL instruments_4_supplied_name String NOx analyzer
attribute NC_GLOBAL keywords String active, ammonia, ammonium, available, bacteria, Bacteria_sd, bco, bco-dmo, beam, Beam_Transmission, biological, chemical, chemistry, chloro, Chloro_sd, chlorophyll, commerce, concentration, concentration_of_chlorophyll_in_sea_water, data, dataset, datetime, day, density, department, depth, dMn_II, dMn_II_sd, dMn_III_L, dMn_III_L_sd, dMn_T, dMn_T_sd, dmo, doc, DOC_sd, downwelling, downwelling_photosynthetic_photon_radiance_in_sea_water, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Optics > Photosynthetically Active Radiation, Earth Science > Oceans > Ocean Optics > Radiance, Earth Science > Oceans > Salinity/Density > Salinity, erddap, florescence, gross, Gross_H2O2, Gross_H2O2_sd, Hg_0, Hg_0_sd, iii, iso, latitude, longitude, management, MnOx, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrate_in_sea_water, mole_concentration_of_nitrite_in_sea_water, month, n02, nanoeuks, Nanoeuks_sd, net, Net_H2O2, Net_H2O2_sd, nh4, nitrate, nitrite, NO2, no3, O2, ocean, oceanography, oceans, office, optics, oxygen, PAR, part, Part_O2, Part_O2_sd, photon, photosynthetic, photosynthetically, picoeuks, Picoeuks_sd, practical, preliminary, radiance, radiation, salinity, science, sea, sea_water_practical_salinity, seawater, sheet, station, synechococcus, Synechococcus_sd, Temp, temperature, time, time2, tot, Tot_O2, Tot_O2_sd, total, Total_dHg, Total_dHg_sd, transmission, water, year
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/765327/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/765327 (external link)
attribute NC_GLOBAL Northernmost_Northing double 41.18026
attribute NC_GLOBAL param_mapping String {'765327': {'lat': 'flag - latitude', 'Depth': 'flag - depth', 'long': 'flag - longitude', 'ISO_datetime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/765327/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_0_affiliation_acronym String WHOI
attribute NC_GLOBAL people_0_person_name String Colleen Hansel
attribute NC_GLOBAL people_0_person_nid String 756932
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of California-Santa Cruz
attribute NC_GLOBAL people_1_affiliation_acronym String UC Santa Cruz
attribute NC_GLOBAL people_1_person_name String Carl Lamborg
attribute NC_GLOBAL people_1_person_nid String 50968
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Mathew Biddle
attribute NC_GLOBAL people_2_person_nid String 708682
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String ROS in Hg Cycling
attribute NC_GLOBAL projects_0_acronym String ROS in Hg Cycling
attribute NC_GLOBAL projects_0_description String NSF Abstract:
Mercury (Hg) is a toxic trace element that bioaccumulates into marine food webs, imposing a health threat to humans through the consumption of seafood. However, controls on the cycling of Hg in the ocean are poorly understood. Most research to date has focused on sun-lit and/or Hg-laden environments, where light-induced chemical and mercury resistance reactions, respectively, have been identified as dominant pathways for Hg cycling. The paradigm that dark Hg reactions are irrelevant is fading and it is now apparent that dark redox reactions, both reduction and oxidation, are important in the cycling of Hg. In this study, researchers at the Woods Hole Oceanographic Institution and Colorado School of Mines will obtain a better understanding of the biogeochemical reactions responsible for dark redox transformations of mercury (Hg) in marine systems. The researchers will explore the relationship between microbial activity, reactive oxygen species, and Hg speciation in a series of laboratory- and field-based investigations to obtain a mechanistic understanding of dark Hg cycling. By identifying new controls on the redox cycling of Hg in the ocean, this research will help inform global and ecosystem models used to predict Hg bioavailability.
Broader Impacts: The proponents plan to educate high school teachers from Boston Green Academy in South Boston on mercury biogeochemistry and have one teacher participate in the summer research cruises, as well as develop science curricula to engage the underrepresented students at the school in science. One postdoc and one graduate student from Woods Hole Oceanographic Institution and one graduate student from the Colorado School of Mines would be supported and trained as part of this project. It is anticipated that undergraduate students would have the opportunity to participate in the study as summer interns.
attribute NC_GLOBAL projects_0_end_date String 2019-07
attribute NC_GLOBAL projects_0_geolocation String Coastal North Atlantic
attribute NC_GLOBAL projects_0_name String Collaborative Research: Defining the Role of Biologically Produced Reactive Oxygen Species in Dark Mercury Cycling
attribute NC_GLOBAL projects_0_project_nid String 756930
attribute NC_GLOBAL projects_0_start_date String 2014-02
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 37.77692
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String Year,Month
attribute NC_GLOBAL summary String Samples were collected from four water column sites along the Northeast Coast of the United States in August 2017 aboard the R/V Endeavor. Samples were collected using a 12-bottle trace metal clean CTD (Conductivity, Temperature and Depth) rosette, and were kept clean by using acid washed tubing to collect water directly into acid-washed 1 L PTFE bottles. Sample depths were chosen based on the water column profile obtained from a separate 24-bottle CTD rosette system equipped with Seabird software, which also provided the temperature, dissolved oxygen, salinity, PAR, beam transmission and fluorescence profiles reported here.
attribute NC_GLOBAL time_coverage_end String 2017-08-28T18:26:40Z
attribute NC_GLOBAL time_coverage_start String 2017-08-20T20:06:38Z
attribute NC_GLOBAL title String Coastal water biogeochemistry collected aboard the R/V Endeavor along the North Atlantic coast from 2017-08-20 to 2017-08-28
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -74.56461
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable sheet   String  
attribute sheet bcodmo_name String unknown
attribute sheet description String Name of the sheet from the original data file
attribute sheet long_name String Sheet
attribute sheet units String unitless
variable Station   byte  
attribute Station _FillValue byte 127
attribute Station actual_range byte 1, 6
attribute Station bcodmo_name String sta
attribute Station description String station identifier
attribute Station long_name String Station
attribute Station units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 37.77692, 41.18026
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String station latitude with north positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -74.56461, -67.92692
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String station longitude with east positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude source_name String long
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Year   short  
attribute Year _FillValue short 32767
attribute Year actual_range short 2017, 2017
attribute Year bcodmo_name String year
attribute Year description String year of collection in yyyy format
attribute Year long_name String Year
attribute Year nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/YEARXXXX/ (external link)
attribute Year units String unitless
variable Month   byte  
attribute Month _FillValue byte 127
attribute Month actual_range byte 8, 8
attribute Month bcodmo_name String month
attribute Month description String month of collection in mm format
attribute Month long_name String Month
attribute Month nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/MNTHXXXX/ (external link)
attribute Month units String unitless
variable Day   byte  
attribute Day _FillValue byte 127
attribute Day actual_range byte 20, 28
attribute Day bcodmo_name String day
attribute Day description String day of collection in dd format
attribute Day long_name String Day
attribute Day nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/ (external link)
attribute Day units String unitless
variable time2   String  
attribute time2 bcodmo_name String time
attribute time2 description String time of collection in HH:MM format
attribute time2 long_name String Time
attribute time2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute time2 units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.503259598E9, 1.5039448E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String date and time following ISO format
attribute time ioos_category String Time
attribute time long_name String ISO Datetime UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String ISO_datetime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 2.016, 2031.208
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String water depth
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable Temp   float  
attribute Temp _FillValue float NaN
attribute Temp actual_range float 3.4031, 25.7564
attribute Temp bcodmo_name String temperature
attribute Temp description String Water temperature from CTD
attribute Temp long_name String Temperature
attribute Temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp units String degrees Celsius (C)
variable Florescence   float  
attribute Florescence _FillValue float NaN
attribute Florescence actual_range float 0.0462, 3.4339
attribute Florescence bcodmo_name String fluorescence
attribute Florescence description String water flourescence from CTD
attribute Florescence long_name String Florescence
attribute Florescence nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute Florescence units String miligrams per meter cubed (mg/m3)
variable Chlorophyll   double  
attribute Chlorophyll _FillValue double NaN
attribute Chlorophyll actual_range double 0.0, 3.175071375
attribute Chlorophyll bcodmo_name String chlorophyll a
attribute Chlorophyll colorBarMaximum double 30.0
attribute Chlorophyll colorBarMinimum double 0.03
attribute Chlorophyll colorBarScale String Log
attribute Chlorophyll description String Chlorophyll a concentration
attribute Chlorophyll long_name String Concentration Of Chlorophyll In Sea Water
attribute Chlorophyll nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chlorophyll units String micrograms per liter (ug/L)
variable Chloro_sd   double  
attribute Chloro_sd _FillValue double NaN
attribute Chloro_sd actual_range double 3.8459253727671276E-16, 0.06770068202943032
attribute Chloro_sd bcodmo_name String chlorophyll a
attribute Chloro_sd colorBarMaximum double 50.0
attribute Chloro_sd colorBarMinimum double 0.0
attribute Chloro_sd description String Chlorophyll a concentration standard deviation
attribute Chloro_sd long_name String Chloro Sd
attribute Chloro_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chloro_sd units String micrograms per liter (ug/L)
variable PAR   float  
attribute PAR _FillValue float NaN
attribute PAR actual_range float -1.12E-4, 2012.6
attribute PAR bcodmo_name String PAR
attribute PAR colorBarMaximum double 70.0
attribute PAR colorBarMinimum double 0.0
attribute PAR description String Photosynthetically active radiation (CTD)
attribute PAR long_name String Downwelling Photosynthetic Photon Radiance In Sea Water
attribute PAR units String microeinteins per meter squared second (microE/m2s)
variable Beam_Transmission   float  
attribute Beam_Transmission _FillValue float NaN
attribute Beam_Transmission actual_range float 71.9022, 86.8471
attribute Beam_Transmission bcodmo_name String transmission
attribute Beam_Transmission description String Beam transmission (CTD)
attribute Beam_Transmission long_name String Beam Transmission
attribute Beam_Transmission units String percent
variable Salinity   float  
attribute Salinity _FillValue float NaN
attribute Salinity actual_range float 31.3729, 36.0649
attribute Salinity bcodmo_name String sal
attribute Salinity colorBarMaximum double 37.0
attribute Salinity colorBarMinimum double 32.0
attribute Salinity description String Water salinity (CTD)
attribute Salinity long_name String Sea Water Practical Salinity
attribute Salinity nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute Salinity units String PSU
variable O2   float  
attribute O2 _FillValue float NaN
attribute O2 actual_range float 2.9535, 6.5497
attribute O2 bcodmo_name String dissolved Oxygen
attribute O2 description String Water oxygen concentration
attribute O2 long_name String O2
attribute O2 units String mililiters per liter (mL/L)
variable NO2   double  
attribute NO2 _FillValue double NaN
attribute NO2 actual_range double 0.0, 0.5662127
attribute NO2 bcodmo_name String NO2
attribute NO2 colorBarMaximum double 1.0
attribute NO2 colorBarMinimum double 0.0
attribute NO2 description String Water nitrite concentration
attribute NO2 long_name String Mole Concentration Of Nitrite In Sea Water
attribute NO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/ (external link)
attribute NO2 units String micromole (uM)
variable NH4   double  
attribute NH4 _FillValue double NaN
attribute NH4 actual_range double 0.0, 2.6446
attribute NH4 bcodmo_name String Ammonium
attribute NH4 colorBarMaximum double 5.0
attribute NH4 colorBarMinimum double 0.0
attribute NH4 description String Water ammonium concentration
attribute NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String micromole (uM)
variable NO3   double  
attribute NO3 _FillValue double NaN
attribute NO3 actual_range double 0.0, 6.746595499
attribute NO3 bcodmo_name String NO3
attribute NO3 colorBarMaximum double 50.0
attribute NO3 colorBarMinimum double 0.0
attribute NO3 description String Water nitrate concentration
attribute NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute NO3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/ (external link)
attribute NO3 units String micromole (uM)
variable Nanoeuks   String  
attribute Nanoeuks bcodmo_name String abundance
attribute Nanoeuks description String Nanoeukaryote abundance
attribute Nanoeuks long_name String Nanoeuks
attribute Nanoeuks nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Nanoeuks units String cells per mililiter (cells/mL)
variable Nanoeuks_sd   String  
attribute Nanoeuks_sd bcodmo_name String abundance
attribute Nanoeuks_sd description String Nanoeukaryote abundance standard deviation
attribute Nanoeuks_sd long_name String Nanoeuks Sd
attribute Nanoeuks_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Nanoeuks_sd units String cells per mililiter (cells/mL)
variable Picoeuks   String  
attribute Picoeuks bcodmo_name String abundance
attribute Picoeuks description String Picoeukayrote abundance
attribute Picoeuks long_name String Picoeuks
attribute Picoeuks nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Picoeuks units String cells per mililiter (cells/mL)
variable Picoeuks_sd   String  
attribute Picoeuks_sd bcodmo_name String abundance
attribute Picoeuks_sd description String Picoeukayrote abundance standard deviation
attribute Picoeuks_sd long_name String Picoeuks Sd
attribute Picoeuks_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Picoeuks_sd units String cells per mililiter (cells/mL)
variable Synechococcus   double  
attribute Synechococcus _FillValue double NaN
attribute Synechococcus actual_range double 216.89821638477133, 359209.4188332472
attribute Synechococcus bcodmo_name String abundance
attribute Synechococcus description String Synechococcus abundance
attribute Synechococcus long_name String Synechococcus
attribute Synechococcus nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Synechococcus units String cells per mililiter (cells/mL)
variable Synechococcus_sd   String  
attribute Synechococcus_sd bcodmo_name String abundance
attribute Synechococcus_sd description String Synechococcus abundance standard deviation
attribute Synechococcus_sd long_name String Synechococcus Sd
attribute Synechococcus_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Synechococcus_sd units String cells per mililiter (cells/mL)
variable Bacteria   double  
attribute Bacteria _FillValue double NaN
attribute Bacteria actual_range double 41416.94360106493, 4188788.3068036768
attribute Bacteria bcodmo_name String abundance
attribute Bacteria description String Bacteria concentration
attribute Bacteria long_name String Bacteria
attribute Bacteria nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Bacteria units String cells per mililiter (cells/mL)
variable Bacteria_sd   double  
attribute Bacteria_sd _FillValue double NaN
attribute Bacteria_sd actual_range double 11174.976795122218, 141799.111136533
attribute Bacteria_sd bcodmo_name String abundance
attribute Bacteria_sd colorBarMaximum double 50.0
attribute Bacteria_sd colorBarMinimum double 0.0
attribute Bacteria_sd description String Bacteria concentration standard deviation
attribute Bacteria_sd long_name String Bacteria Sd
attribute Bacteria_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Bacteria_sd units String cells per mililiter (cells/mL)
variable DOC   double  
attribute DOC _FillValue double NaN
attribute DOC actual_range double 0.5609000000000001, 1.4446666666666665
attribute DOC bcodmo_name String DOC
attribute DOC description String Dissolved organic carbon
attribute DOC long_name String DOC
attribute DOC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC units String miligrams per liter (mg/L)
variable DOC_sd   double  
attribute DOC_sd _FillValue double NaN
attribute DOC_sd actual_range double 0.018193405398660236, 0.17077330978034413
attribute DOC_sd bcodmo_name String DOC
attribute DOC_sd colorBarMaximum double 50.0
attribute DOC_sd colorBarMinimum double 0.0
attribute DOC_sd description String Dissolved organic carbon standard deviation
attribute DOC_sd long_name String DOC Sd
attribute DOC_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC_sd units String miligrams per liter (mg/L)
variable Tot_O2   double  
attribute Tot_O2 _FillValue double NaN
attribute Tot_O2 actual_range double 10.60227148330597, 2362.1644493177378
attribute Tot_O2 bcodmo_name String unknown
attribute Tot_O2 description String Total superoxide
attribute Tot_O2 long_name String Tot O2
attribute Tot_O2 units String picomole (pM)
variable Tot_O2_sd   double  
attribute Tot_O2_sd _FillValue double NaN
attribute Tot_O2_sd actual_range double 0.0, 415.79304171248594
attribute Tot_O2_sd bcodmo_name String unknown
attribute Tot_O2_sd colorBarMaximum double 50.0
attribute Tot_O2_sd colorBarMinimum double 0.0
attribute Tot_O2_sd description String Total superoxide standard deviation
attribute Tot_O2_sd long_name String Tot O2 Sd
attribute Tot_O2_sd units String picomole (pM)
variable Part_O2   double  
attribute Part_O2 _FillValue double NaN
attribute Part_O2 actual_range double 0.8597278761411509, 2462.811999999999
attribute Part_O2 bcodmo_name String unknown
attribute Part_O2 description String Particle associated superoxide
attribute Part_O2 long_name String Part O2
attribute Part_O2 units String picomole (pM)
variable Part_O2_sd   double  
attribute Part_O2_sd _FillValue double NaN
attribute Part_O2_sd actual_range double 0.0, 415.7930417124871
attribute Part_O2_sd bcodmo_name String unknown
attribute Part_O2_sd colorBarMaximum double 50.0
attribute Part_O2_sd colorBarMinimum double 0.0
attribute Part_O2_sd description String Particle associated superoxide standard deviation
attribute Part_O2_sd long_name String Part O2 Sd
attribute Part_O2_sd units String picomole (pM)
variable Net_H2O2   double  
attribute Net_H2O2 _FillValue double NaN
attribute Net_H2O2 actual_range double 4.379275135171688, 257.0328945421757
attribute Net_H2O2 bcodmo_name String Hydrogen Peroxide
attribute Net_H2O2 description String Net hydrogen peroxide
attribute Net_H2O2 long_name String Net H2 O2
attribute Net_H2O2 units String nanomole (nM)
variable Net_H2O2_sd   double  
attribute Net_H2O2_sd _FillValue double NaN
attribute Net_H2O2_sd actual_range double 1.625384364522924, 204.67434195024987
attribute Net_H2O2_sd bcodmo_name String Hydrogen Peroxide
attribute Net_H2O2_sd colorBarMaximum double 50.0
attribute Net_H2O2_sd colorBarMinimum double 0.0
attribute Net_H2O2_sd description String Net hydrogen peroxide standard deviation
attribute Net_H2O2_sd long_name String Net H2 O2 Sd
attribute Net_H2O2_sd units String nanomole (nM)
variable Gross_H2O2   double  
attribute Gross_H2O2 _FillValue double NaN
attribute Gross_H2O2 actual_range double 9.384161003939033, 236.07968842897944
attribute Gross_H2O2 bcodmo_name String Hydrogen Peroxide
attribute Gross_H2O2 description String Gross hydrogen peroxide
attribute Gross_H2O2 long_name String Gross H2 O2
attribute Gross_H2O2 units String nanomole (nM)
variable Gross_H2O2_sd   double  
attribute Gross_H2O2_sd _FillValue double NaN
attribute Gross_H2O2_sd actual_range double 0.002632328618744691, 88.36608183688755
attribute Gross_H2O2_sd bcodmo_name String Hydrogen Peroxide
attribute Gross_H2O2_sd colorBarMaximum double 50.0
attribute Gross_H2O2_sd colorBarMinimum double 0.0
attribute Gross_H2O2_sd description String Gross hydrogen peroxide standard deviation
attribute Gross_H2O2_sd long_name String Gross H2 O2 Sd
attribute Gross_H2O2_sd units String nanomole (nM)
variable Total_dHg   double  
attribute Total_dHg _FillValue double NaN
attribute Total_dHg actual_range double 0.41351546121593363, 2.467451519129822
attribute Total_dHg bcodmo_name String trace_metal_conc
attribute Total_dHg description String Total dissolved Hg
attribute Total_dHg long_name String Total D Hg
attribute Total_dHg nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Total_dHg units String picomole (pM)
variable Total_dHg_sd   double  
attribute Total_dHg_sd _FillValue double NaN
attribute Total_dHg_sd actual_range double 0.001644542107712444, 1.188640589545043
attribute Total_dHg_sd bcodmo_name String trace_metal_conc
attribute Total_dHg_sd colorBarMaximum double 50.0
attribute Total_dHg_sd colorBarMinimum double 0.0
attribute Total_dHg_sd description String Total dissolved Hg standard deviation
attribute Total_dHg_sd long_name String Total D Hg Sd
attribute Total_dHg_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Total_dHg_sd units String picomole (pM)
variable Hg_0   double  
attribute Hg_0 _FillValue double NaN
attribute Hg_0 actual_range double 0.05494494055154578, 0.30003286883856095
attribute Hg_0 bcodmo_name String trace_metal_conc
attribute Hg_0 description String Elemental Hg
attribute Hg_0 long_name String HG 0
attribute Hg_0 nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Hg_0 units String picomole (pM)
variable Hg_0_sd   double  
attribute Hg_0_sd _FillValue double NaN
attribute Hg_0_sd actual_range double 2.9741736172874837E-4, 0.07440280528199449
attribute Hg_0_sd bcodmo_name String trace_metal_conc
attribute Hg_0_sd colorBarMaximum double 50.0
attribute Hg_0_sd colorBarMinimum double 0.0
attribute Hg_0_sd description String Elemental Hg standard deviation
attribute Hg_0_sd long_name String HG 0 SD
attribute Hg_0_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Hg_0_sd units String picomole (pM)
variable dMn_II   float  
attribute dMn_II _FillValue float NaN
attribute dMn_II actual_range float 0.0, 40.9
attribute dMn_II bcodmo_name String Mn
attribute dMn_II description String Dissolved Mn(II)
attribute dMn_II long_name String D Mn II
attribute dMn_II units String nanomole (nM)
variable dMn_II_sd   float  
attribute dMn_II_sd _FillValue float NaN
attribute dMn_II_sd actual_range float 0.0, 7.55
attribute dMn_II_sd bcodmo_name String Mn
attribute dMn_II_sd colorBarMaximum double 50.0
attribute dMn_II_sd colorBarMinimum double 0.0
attribute dMn_II_sd description String Dissolved Mn(II) standard deviation
attribute dMn_II_sd long_name String D Mn II Sd
attribute dMn_II_sd units String nanomole (nM)
variable dMn_T   float  
attribute dMn_T _FillValue float NaN
attribute dMn_T actual_range float 6.19, 152.7
attribute dMn_T bcodmo_name String Mn
attribute dMn_T description String Total dissolved Mn
attribute dMn_T long_name String D MN T
attribute dMn_T units String nanomole (nM)
variable dMn_T_sd   float  
attribute dMn_T_sd _FillValue float NaN
attribute dMn_T_sd actual_range float 0.08, 18.73
attribute dMn_T_sd bcodmo_name String Mn
attribute dMn_T_sd colorBarMaximum double 50.0
attribute dMn_T_sd colorBarMinimum double 0.0
attribute dMn_T_sd description String Total dissolved Mn standard deviation
attribute dMn_T_sd long_name String D MN T SD
attribute dMn_T_sd units String nanomole (nM)
variable dMn_III_L   float  
attribute dMn_III_L _FillValue float NaN
attribute dMn_III_L actual_range float 0.0, 147.49
attribute dMn_III_L bcodmo_name String Mn
attribute dMn_III_L description String Dissolved Mn(III)-L
attribute dMn_III_L long_name String D Mn III L
attribute dMn_III_L units String nanomole (nM)
variable dMn_III_L_sd   float  
attribute dMn_III_L_sd _FillValue float NaN
attribute dMn_III_L_sd actual_range float 0.16, 13.31
attribute dMn_III_L_sd bcodmo_name String Mn
attribute dMn_III_L_sd colorBarMaximum double 50.0
attribute dMn_III_L_sd colorBarMinimum double 0.0
attribute dMn_III_L_sd description String Dissolved Mn(III)-L standard deviation
attribute dMn_III_L_sd long_name String D Mn III L Sd
attribute dMn_III_L_sd units String nanomole (nM)
variable MnOx   float  
attribute MnOx _FillValue float NaN
attribute MnOx actual_range float 0.02, 3.85
attribute MnOx bcodmo_name String Mn
attribute MnOx description String Mn oxides
attribute MnOx long_name String Mn Ox
attribute MnOx units String nanomole

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