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     data   graph     files  public [Nitrogen and carbon fixation rates - Trichodesmium] - Nitrogen and carbon fixation rates
from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018 (Collaborative
Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic
ocean)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_766354

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Shipboard incubation experiments were carried out at 15 stations in the
tropical Atlantic with trace metal clean-collected Trichodesmium colonies to
examine iron (Fe) and phosphorus (P) co-limitation of dinitrogen (N2) fixation
(see TriCoLim Station coordinates metadata). Treatments were: Co-limited
control (no additions, \u201cColimit\u201d), + 2nM Fe (\u201cFe\u201d), + 50
nM P (\u201cP\u201d) and Replete (+Fe+P, \u201cRep\u201d).\u00a0All
incubations were carried out at ambient (\u201cLo\u201d) temperature and
ambient temperature +3C (\u201cHi\u201d) for 2-4 days on deck before measuring
15N2 and 13CO2 fixation rates and particulate organic carbon (POC) and
nitrogen (PON). See related datasets.
[Nitrogen and carbon fixation rates - bulk seawater](\\"https://www.bco-
dmo.org/dataset/766334\\") | Nitrogen and carbon fixation rates from bulk
water samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018
---|---
[POC and PON - bulk seawater](\\"https://www.bco-dmo.org/dataset/766374\\") |
POC and PON from bulk seawater samples from RV/Atlantic cruise AT39-05, Feb-
Mar 2018
[POC and PON - Trichodesmium](\\"https://www.bco-dmo.org/dataset/766394\\") |
POC and PON from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-
Mar 2018
attribute NC_GLOBAL awards_0_award_nid String 724450
attribute NC_GLOBAL awards_0_award_number String OCE-1657757
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1657757 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Nitrogen and carbon fixation rates from Trichodesmium
from RV/Atlantic cruise AT39-05, Feb-Mar 2018
PI's: D. Hutchins, E. Webb (USC)
version date: 2019-10-02
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-05-06T14:11:11Z
attribute NC_GLOBAL date_modified String 2020-05-04T13:04:35Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.766354.1
attribute NC_GLOBAL Easternmost_Easting double -22.0019
attribute NC_GLOBAL geospatial_lat_max double 16.0877
attribute NC_GLOBAL geospatial_lat_min double -5.1159
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -22.0019
attribute NC_GLOBAL geospatial_lon_min double -57.4196
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/766354 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String TM Bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect clean seawater.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 766361
attribute NC_GLOBAL instruments_0_description String Trace metal (TM) clean rosette bottle used for collecting trace metal clean seawater samples.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/30/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Trace Metal Bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 493
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, C_fix, chemical, data, dataset, dmo, erddap, experiment, Experiment_ID, fix, latitude, longitude, management, N2_fix, oceanography, office, preliminary, sample, Sample_D, station, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/766354/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/766354 (external link)
attribute NC_GLOBAL Northernmost_Northing double 16.0877
attribute NC_GLOBAL param_mapping String {'766354': {'Latitude': 'flag - latitude', 'Longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/766354/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Southern California
attribute NC_GLOBAL people_0_affiliation_acronym String USC
attribute NC_GLOBAL people_0_person_name String David A. Hutchins
attribute NC_GLOBAL people_0_person_nid String 51048
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Southern California
attribute NC_GLOBAL people_1_affiliation_acronym String USC
attribute NC_GLOBAL people_1_person_name String Eric A Webb
attribute NC_GLOBAL people_1_person_nid String 51340
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String TriCoLim
attribute NC_GLOBAL projects_0_acronym String TriCoLim
attribute NC_GLOBAL projects_0_description String NSF abstract:
Marine cyanobacteria are able to use or "fix" atmospheric nitrogen gas, and so supply much of the essential nutrient nitrogen that supports open ocean food chains. Oceanographers have usually thought that the growth of these nitrogen-fixing cyanobacteria is limited at any particular time and place by the supply of either iron, or of phosphorus. Preliminary experiments have shown, though, that these nitrogen fixers instead grow best when both iron and phosphorus are scarce at the same time. In this project, the researchers will use cellular indicators that are specific for iron and phosphorus limitation to determine how important this type of "balanced limitation" of nitrogen-fixing cyanobacteria is in controlling the productivity of ocean food chains in the tropical Atlantic Ocean. Two graduate students will be trained at the University of Southern California (USC) and Woods Hole Oceanographic Institution, as well as a postdoctoral researcher at USC. Educational outreach efforts will take place at a Los Angeles inner city high school with a student body that is over 98% Hispanic and African-American, and with underrepresented undergraduates in the USC Global Environmental Microbiology course. In addition, two Research Experiences for Undergraduates students will be supervised for summer research projects to help them learn about science career options.
The researchers will investigate the biological and biogeochemical consequences of this unique balanced iron/phosphorus-limited phenotype, using both laboratory and fieldwork approaches. During the first year of this project, the nitrogen-fixing cyanobacteria will be cultured under iron and/or phosphorus limitation, followed by application of proteomics and transcriptomics to identify genes that are potential diagnostic biomarkers for iron/phosphorus balanced limitation. Preliminary work has already identified one promising candidate biomarker in one cyanobacterium, an EzrA protein domain that appears to be associated with the cell size decreases seen specifically under balanced limitation, and the researchers have identified numerous other potential candidates for similar biomarkers. During the second year, these new co-limitation biomarkers and others previously validated for iron limitation (IsiB) and phosphorus limitation (SphX) will be used to investigate balanced limitation during a research cruise transecting from relatively high-iron, low-phosphorus North Atlantic waters, to the relatively high-phosphorus, low-iron South Atlantic. This fieldwork component will survey nitrogen fixing cyanobacteria populations across this natural iron/phosphorus gradient for genetic, proteomic, and physiological indicators of balanced limitation, as well as testing their responses to iron and phosphorus manipulations in shipboard incubation experiments. The third year will be devoted to sample analysis, and publications exploring the responses of oceanic nitrogen fixers to simultaneous limitation by both iron and phosphorus.
attribute NC_GLOBAL projects_0_end_date String 2020-02
attribute NC_GLOBAL projects_0_geolocation String Tropical Atlantic
attribute NC_GLOBAL projects_0_name String Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
attribute NC_GLOBAL projects_0_project_nid String 724451
attribute NC_GLOBAL projects_0_start_date String 2017-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -5.1159
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Nitrogen and carbon fixation rates from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018.
attribute NC_GLOBAL title String [Nitrogen and carbon fixation rates - Trichodesmium] - Nitrogen and carbon fixation rates from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018 (Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -57.4196
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Station   byte  
attribute Station _FillValue byte 127
attribute Station actual_range byte 3, 19
attribute Station bcodmo_name String station
attribute Station description String station identifier
attribute Station long_name String Station
attribute Station units String unitless
variable Experiment_ID   String  
attribute Experiment_ID bcodmo_name String exp_id
attribute Experiment_ID description String experiment_id identifier
attribute Experiment_ID long_name String Experiment ID
attribute Experiment_ID units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -5.1159, 16.0877
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -57.4196, -22.0019
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Sample_D   String  
attribute Sample_D bcodmo_name String sample
attribute Sample_D description String sample identifier
attribute Sample_D long_name String Sample D
attribute Sample_D nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_D units String unitless
variable Treatment   String  
attribute Treatment bcodmo_name String treatment
attribute Treatment description String treatment:
Lo temp = ambient temperature
Hi temp = ambient temperature plus 3 degrees C.
Colimit = Co-limited control (no additions)
Fe = 2nM Fe
P = 50 nM P
Rep =  Replete (+Fe+P)
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable N2_fix   float  
attribute N2_fix _FillValue float NaN
attribute N2_fix actual_range float -0.00298, 0.0153
attribute N2_fix bcodmo_name String N2_fix
attribute N2_fix description String nitrogen fixation rate measured as 15N2
attribute N2_fix long_name String N2 Fix
attribute N2_fix units String micromol/liter/hour (umol/l/h)
variable C_fix   float  
attribute C_fix _FillValue float NaN
attribute C_fix actual_range float 0.00725, 1.06
attribute C_fix bcodmo_name String unknown
attribute C_fix description String carbon fixation rate measured as 13CO2
attribute C_fix long_name String C Fix
attribute C_fix units String micromol/liter/hour (umol/l/h)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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