ERDDAP > info > bcodmo_dataset_771333

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Series 3A: DIC] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Dissolved Inorganic Carbon (DIC) measurements (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton)		I   M	background			BCO-DMO	bcodmo_dataset_771333

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm respectively. For each CO2 concentration, four temperatures were tested: 15 degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each temperature, three light levels were tested: a sub-optimum light (SOL) intensity of 60 umol photons \u00b7 m-2 \u00b7 s-1, an optimum light (OL) intensity of 400 umol photons \u00b7 m-2 \u00b7 s-1 and an extreme light (EL) intensity of 800 umol photons \u00b7 m-2 \u00b7 s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at two temperatures (either 15 degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running simultaneously. This was repeated for each CO2 concentration. Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test- tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from <0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 4 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially- growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize the effects of diurnal cycles. Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol \u00b7 kg-1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP 1014 (TP1014) stock culture at the start of the experiments. Dissolved Inorganic Carbon (DIC) measurements: DIC was measured in freshly prepared media, and at the end of the experiment phase. 25 ml of the sample was siphoned into clean glass serum vials, fixed with HgCL2 (0.035% final conc. v/v), and sealed with butyl rubber septa. Samples were stored at 4 degrees-C until analyzed. Prior experiments had confirmed that no gas exchange, and/or change in DIC occurred during sample storage for up to 30 days using this method. Total dissolved inorganic carbon (TCO2) samples were analyzed using an automated infrared inorganic carbon analyzer (AIRICA).\u00a0 The AIRICA-23 (MARIANDA, Kiel, Germany), is a high precision instrument used to measure total dissolved inorganic carbon in seawater. The system uses a high precision syringe and a mass flow controller to deliver a known volume of sample into a stripper where it is then acidified, converting the inorganic carbon species into CO2 and delivered under constant flow to nondispersive infrared detector. The CO2 is then carried using an inert reference gas (N2) into a LICOR-7000 that measures pCO2 using the difference in infrared absorbance between a sample and reference cell. The pCO2 is recorded over time and integrated by the AIRICA software. This integrated value is proportional to the amount of dissolved inorganic carbon evolved from the sample and converted to carbon units using a conversion factor (CT Factor). The CT Factor is determined by calibration of the system against a certified reference material of known value (Dickson et al. 2007. Guide to Best Practices for Ocean CO2 Measurements). The value is converted to gravimetric units (umol/kg) using the volume, temperature and salinity of the sample. In order to check for analytical stability of the system throughout a run, a certified reference material is used in between every 5 samples. Replicate DIC measurements were averaged. Problem report:\u00a0DIC measurements for two treatments (410 ppm CO2, 20\u00b0C), and (410 ppm CO2, 20\u00b0C) were flawed. \u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	654346
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1538602
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Series 3A-1: Multiple stressor experiments on T. pseudonana (CCMP1014): Dissolved Inorganic carbon (DIC) PI: U. Passow, N. D'Souza (UCSB), E. Laws (LSU) version date: 2019-06-17
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2019-06-19T19:26:47Z
attribute	NC_GLOBAL	date_modified	String	2020-06-29T12:52:45Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.771333.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/771333
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used for incubation of TP1014 cultures.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	771341
attribute	NC_GLOBAL	instruments_0_description	String	An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Cell Cultivator
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	714540
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	An Automated infrared inorganic carbon analyzer (AIRICA) for analysis of dissolved inorganic carbon.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	771350
attribute	NC_GLOBAL	instruments_1_description	String	Instruments measuring carbonate in sediments and inorganic carbon in the water column.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Inorganic Carbon Analyzers
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	743387
attribute	NC_GLOBAL	instruments_1_supplied_name	String	AIRICA-23 (MARIANDA, Kiel, Germany)
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	A CO2/H2O Analyzer for analysis of dissolved inorganic carbon.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	771354
attribute	NC_GLOBAL	instruments_2_description	String	Instruments measuring carbonate in sediments and inorganic carbon in the water column.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Inorganic Carbon Analyzers
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	743387
attribute	NC_GLOBAL	instruments_2_supplied_name	String	LICOR-7000
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, data, dataset, day, dioxide, dmo, EL_A, EL_B, EL_C, erddap, management, oceanography, office, OL_A, OL_B, phase, preliminary, replicate, sol, SOL_A, SOL_B, SOL_C, Temp, temperature
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/771333/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/771333
attribute	NC_GLOBAL	param_mapping	String	{'771333': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/771333/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_0_person_name	String	Uta Passow
attribute	NC_GLOBAL	people_0_person_nid	String	51317
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Louisiana State University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	LSU-SC&E
attribute	NC_GLOBAL	people_1_person_name	String	Dr Edward Laws
attribute	NC_GLOBAL	people_1_person_nid	String	50767
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_2_person_name	String	Nigel D'Souza
attribute	NC_GLOBAL	people_2_person_nid	String	748936
attribute	NC_GLOBAL	people_2_role	String	Scientist
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_3_person_name	String	Nigel D'Souza
attribute	NC_GLOBAL	people_3_person_nid	String	748936
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_4_person_nid	String	50396
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Stressors on Marine Phytoplankton
attribute	NC_GLOBAL	projects_0_acronym	String	Stressors on Marine Phytoplankton
attribute	NC_GLOBAL	projects_0_description	String	The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience. Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-09
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
attribute	NC_GLOBAL	projects_0_project_nid	String	654347
attribute	NC_GLOBAL	projects_0_start_date	String	2015-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of Dissolved Inorganic Carbon (DIC) made over the course of the experiments.
attribute	NC_GLOBAL	title	String	[Series 3A: DIC] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Dissolved Inorganic Carbon (DIC) measurements (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	Phase		String
attribute	Phase	bcodmo_name	String	sample_descrip
attribute	Phase	description	String	Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.
attribute	Phase	long_name	String	Phase
attribute	Phase	units	String	unitless
variable	CO2		short
attribute	CO2	_FillValue	short	32767
attribute	CO2	actual_range	short	410, 1000
attribute	CO2	bcodmo_name	String	pCO2
attribute	CO2	description	String	Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment
attribute	CO2	long_name	String	CO2
attribute	CO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	CO2	units	String	parts per million (ppm)
variable	Temp		byte
attribute	Temp	_FillValue	byte	127
attribute	Temp	actual_range	byte	15, 30
attribute	Temp	bcodmo_name	String	temperature
attribute	Temp	description	String	Indicates the temperature at which the samples were incubated.
attribute	Temp	long_name	String	Temperature
attribute	Temp	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	Temp	units	String	degrees Celsius
variable	Day		String
attribute	Day	bcodmo_name	String	days
attribute	Day	description	String	Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.
attribute	Day	long_name	String	Day
attribute	Day	units	String	unitless
variable	Replicate		byte
attribute	Replicate	_FillValue	byte	127
attribute	Replicate	actual_range	byte	1, 2
attribute	Replicate	bcodmo_name	String	replicate
attribute	Replicate	description	String	Indicates replication within a treatment. "NA" indicates "not applicable"
attribute	Replicate	long_name	String	Replicate
attribute	Replicate	units	String	unitless
variable	SOL_A		float
attribute	SOL_A	_FillValue	float	NaN
attribute	SOL_A	actual_range	float	1048.68, 2388.51
attribute	SOL_A	bcodmo_name	String	DIC
attribute	SOL_A	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at sub optimum light (SOL)
attribute	SOL_A	long_name	String	SOL A
attribute	SOL_A	units	String	micromol/kilogram
variable	SOL_B		float
attribute	SOL_B	_FillValue	float	NaN
attribute	SOL_B	actual_range	float	1048.68, 2384.16
attribute	SOL_B	bcodmo_name	String	DIC
attribute	SOL_B	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at sub optimum light (SOL)
attribute	SOL_B	long_name	String	SOL B
attribute	SOL_B	units	String	micromol/kilogram
variable	SOL_C		float
attribute	SOL_C	_FillValue	float	NaN
attribute	SOL_C	actual_range	float	1048.68, 2382.33
attribute	SOL_C	bcodmo_name	String	DIC
attribute	SOL_C	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate C incubated at sub optimum light (SOL)
attribute	SOL_C	long_name	String	SOL C
attribute	SOL_C	units	String	micromol/kilogram
variable	OL_A		float
attribute	OL_A	_FillValue	float	NaN
attribute	OL_A	actual_range	float	1048.68, 2605.66
attribute	OL_A	bcodmo_name	String	DIC
attribute	OL_A	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at optimum light (OL)
attribute	OL_A	long_name	String	OL A
attribute	OL_A	units	String	micromol/kilogram
variable	OL_B		float
attribute	OL_B	_FillValue	float	NaN
attribute	OL_B	actual_range	float	1048.68, 2649.36
attribute	OL_B	bcodmo_name	String	DIC
attribute	OL_B	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at optimum light (OL)
attribute	OL_B	long_name	String	OL B
attribute	OL_B	units	String	micromol/kilogram
variable	EL_A		float
attribute	EL_A	_FillValue	float	NaN
attribute	EL_A	actual_range	float	1048.68, 2810.23
attribute	EL_A	bcodmo_name	String	DIC
attribute	EL_A	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at extreme light (EL)
attribute	EL_A	long_name	String	EL A
attribute	EL_A	units	String	micromol/kilogram
variable	EL_B		float
attribute	EL_B	_FillValue	float	NaN
attribute	EL_B	actual_range	float	1048.68, 2835.75
attribute	EL_B	bcodmo_name	String	DIC
attribute	EL_B	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at extreme light (EL)
attribute	EL_B	long_name	String	EL B
attribute	EL_B	units	String	micromol/kilogram
variable	EL_C		float
attribute	EL_C	_FillValue	float	NaN
attribute	EL_C	actual_range	float	1048.68, 2766.09
attribute	EL_C	bcodmo_name	String	DIC
attribute	EL_C	description	String	Dissolved Inorganic Carbon (DIC) measurements in replicate C incubated at extreme light (EL)
attribute	EL_C	long_name	String	EL C
attribute	EL_C	units	String	micromol/kilogram

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.