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Title Sum-
Institution Dataset ID
     data   graph     files  public Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Dissolved Inorganic
Carbon (DIC) measurements
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm
respectively. For each CO2 concentration, four temperatures were tested: 15
degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each
temperature, three light levels were tested: a sub-optimum light (SOL)
intensity of 60 umol photons \u00b7 m-2 \u00b7 s-1, an optimum light (OL)
intensity of 400 umol photons \u00b7 m-2 \u00b7 s-1 and an extreme light (EL)
intensity of 800 umol photons \u00b7 m-2 \u00b7 s-1. All lights were set at a
12 h day: 12 h dark cycle. For logistical reasons, experiments were partially
conducted in series, with all light treatments at two temperatures (either 15
degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running
simultaneously. This was repeated for each CO2 concentration.

Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems
Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-
tubes immersed in a thermostated water bath, each independently illuminated by
an array of cool white LEDs set at specific intensity and timing. A 0.2um
filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile
artificial seawater, and the humidified gas mix was supplied to each tube via
gentle sparging through a 2um stainless steel diffuser. Flow rates were
gradually increased over the course of the incubation to compensate for the
DIC uptake of actively growing cells, and ranged from <0.04 Liters per minute
(LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days.
For each CO2 and temperature level, replication was achieved by incubating
three tubes at sub-optimum light intensities, two tubes at optimum light
intensity, and three tubes at extreme light intensities. Each experiment was
split into two phases: An acclimation phase spanning 4 days, was used to
acclimate cultures to their new environment. Pre-acclimated, exponentially-
growing cultures were then inoculated into fresh media and incubated through a
3-day experimental phase during which assessments of growth, photophysiology,
and nutrient cycling were carried out daily. All sampling started 5 hours into
the daily light cycle to minimize the effects of diurnal cycles.

Experiments were conducted with artificial seawater (ASW) prepared using
previously described methods (Kester et. al 1967), and enriched with nitrate
(NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the
cultures would remain nutrient-replete over the course of the experiment.
Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected
DIC concentration and pH of the growth media was determined for the different
pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with
constants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and
inputs of temperature, salinity, total alkalinity (2376.5 umol \u00b7 kg-1),
pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each
phase of the experiments were manipulated by the addition of NaHCO3, and was
then maintained by bubbling a CO2-Air mix through the cultures over the course
of the experiments. The pH of the growth media was measured
spectrophometrically using the m-cresol purple method (Dickson 1993), and
adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml
aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP
1014 (TP1014) stock culture at the start of the experiments.

Dissolved Inorganic Carbon (DIC) measurements:
DIC was measured in freshly prepared media, and at the end of the experiment
phase. 25 ml of the sample was siphoned into clean glass serum vials, fixed
with HgCL2 (0.035% final conc. v/v), and sealed with butyl rubber septa.
Samples were stored at 4 degrees-C until analyzed. Prior experiments had
confirmed that no gas exchange, and/or change in DIC occurred during sample
storage for up to 30 days using this method. Total dissolved inorganic carbon
(TCO2) samples were analyzed using an automated infrared inorganic carbon
analyzer (AIRICA).\u00a0 The AIRICA-23 (MARIANDA, Kiel, Germany), is a high
precision instrument used to measure total dissolved inorganic carbon in
seawater. The system uses a high precision syringe and a mass flow controller
to deliver a known volume of sample into a stripper where it is then
acidified, converting the inorganic carbon species into CO2 and delivered
under constant flow to nondispersive infrared detector. The CO2 is then
carried using an inert reference gas (N2) into a LICOR-7000 that measures pCO2
using the difference in infrared absorbance between a sample and reference
cell. The pCO2 is recorded over time and integrated by the AIRICA software.
This integrated value is proportional to the amount of dissolved inorganic
carbon evolved from the sample and converted to carbon units using a
conversion factor (CT Factor). The CT Factor is determined by calibration of
the system against a certified reference material of known value (Dickson et
al. 2007. Guide to Best Practices for Ocean CO2 Measurements). The value is
converted to gravimetric units (umol/kg) using the volume, temperature and
salinity of the sample. In order to check for analytical stability of the
system throughout a run, a certified reference material is used in between
every 5 samples. Replicate DIC measurements were averaged.

Problem report:\u00a0DIC measurements for two treatments (410 ppm CO2,
20\u00b0C), and (410 ppm CO2, 20\u00b0C) were flawed.

attribute NC_GLOBAL awards_0_award_nid String 654346
attribute NC_GLOBAL awards_0_award_number String OCE-1538602
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Series 3A-1: Multiple stressor experiments on T. pseudonana (CCMP1014): Dissolved Inorganic carbon (DIC)
PI: U. Passow, N. D'Souza (UCSB), E. Laws (LSU)
version date: 2019-06-17
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-06-19T19:26:47Z
attribute NC_GLOBAL date_modified String 2020-06-29T12:52:45Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.771333.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/771333 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used for incubation of TP1014 cultures.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 771341
attribute NC_GLOBAL instruments_0_description String An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
attribute NC_GLOBAL instruments_0_instrument_name String Cell Cultivator
attribute NC_GLOBAL instruments_0_instrument_nid String 714540
attribute NC_GLOBAL instruments_0_supplied_name String Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
attribute NC_GLOBAL instruments_1_dataset_instrument_description String An Automated infrared inorganic carbon analyzer (AIRICA) for analysis of dissolved inorganic carbon.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 771350
attribute NC_GLOBAL instruments_1_description String Instruments measuring carbonate in sediments and inorganic carbon in the water column.
attribute NC_GLOBAL instruments_1_instrument_name String Inorganic Carbon Analyzers
attribute NC_GLOBAL instruments_1_instrument_nid String 743387
attribute NC_GLOBAL instruments_1_supplied_name String AIRICA-23 (MARIANDA, Kiel, Germany)
attribute NC_GLOBAL instruments_2_dataset_instrument_description String A CO2/H2O Analyzer for analysis of dissolved inorganic carbon.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 771354
attribute NC_GLOBAL instruments_2_description String Instruments measuring carbonate in sediments and inorganic carbon in the water column.
attribute NC_GLOBAL instruments_2_instrument_name String Inorganic Carbon Analyzers
attribute NC_GLOBAL instruments_2_instrument_nid String 743387
attribute NC_GLOBAL instruments_2_supplied_name String LICOR-7000
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, data, dataset, day, dioxide, dmo, EL_A, EL_B, EL_C, erddap, management, oceanography, office, OL_A, OL_B, phase, preliminary, replicate, sol, SOL_A, SOL_B, SOL_C, Temp, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/771333/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/771333 (external link)
attribute NC_GLOBAL param_mapping String {'771333': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/771333/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_0_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_0_person_name String Uta Passow
attribute NC_GLOBAL people_0_person_nid String 51317
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Louisiana State University
attribute NC_GLOBAL people_1_affiliation_acronym String LSU-SC&E
attribute NC_GLOBAL people_1_person_name String Dr Edward Laws
attribute NC_GLOBAL people_1_person_nid String 50767
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_2_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_2_person_name String Nigel D'Souza
attribute NC_GLOBAL people_2_person_nid String 748936
attribute NC_GLOBAL people_2_role String Scientist
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_3_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_3_person_name String Nigel D'Souza
attribute NC_GLOBAL people_3_person_nid String 748936
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_acronym String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_description String The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.
Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.
attribute NC_GLOBAL projects_0_end_date String 2018-09
attribute NC_GLOBAL projects_0_name String Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_project_nid String 654347
attribute NC_GLOBAL projects_0_start_date String 2015-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of Dissolved Inorganic Carbon (DIC) made over the course of the experiments.
attribute NC_GLOBAL title String Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Dissolved Inorganic Carbon (DIC) measurements
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Phase   String  
attribute Phase bcodmo_name String sample_descrip
attribute Phase description String Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.
attribute Phase long_name String Phase
attribute Phase units String unitless
variable CO2   short  
attribute CO2 _FillValue short 32767
attribute CO2 actual_range short 410, 1000
attribute CO2 bcodmo_name String pCO2
attribute CO2 description String Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment
attribute CO2 long_name String CO2
attribute CO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute CO2 units String parts per million (ppm)
variable Temp   byte  
attribute Temp _FillValue byte 127
attribute Temp actual_range byte 15, 30
attribute Temp bcodmo_name String temperature
attribute Temp description String Indicates the temperature at which the samples were incubated.
attribute Temp long_name String Temperature
attribute Temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp units String degrees Celsius
variable Day   String  
attribute Day bcodmo_name String days
attribute Day description String Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.
attribute Day long_name String Day
attribute Day units String unitless
variable Replicate   byte  
attribute Replicate _FillValue byte 127
attribute Replicate actual_range byte 1, 2
attribute Replicate bcodmo_name String replicate
attribute Replicate description String Indicates replication within a treatment. "NA" indicates "not applicable"
attribute Replicate long_name String Replicate
attribute Replicate units String unitless
variable SOL_A   float  
attribute SOL_A _FillValue float NaN
attribute SOL_A actual_range float 1048.68, 2388.51
attribute SOL_A bcodmo_name String DIC
attribute SOL_A description String Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at sub optimum light (SOL)
attribute SOL_A long_name String SOL A
attribute SOL_A units String micromol/kilogram
variable SOL_B   float  
attribute SOL_B _FillValue float NaN
attribute SOL_B actual_range float 1048.68, 2384.16
attribute SOL_B bcodmo_name String DIC
attribute SOL_B description String Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at sub optimum light (SOL)
attribute SOL_B long_name String SOL B
attribute SOL_B units String micromol/kilogram
variable SOL_C   float  
attribute SOL_C _FillValue float NaN
attribute SOL_C actual_range float 1048.68, 2382.33
attribute SOL_C bcodmo_name String DIC
attribute SOL_C description String Dissolved Inorganic Carbon (DIC) measurements in replicate C incubated at sub optimum light (SOL)
attribute SOL_C long_name String SOL C
attribute SOL_C units String micromol/kilogram
variable OL_A   float  
attribute OL_A _FillValue float NaN
attribute OL_A actual_range float 1048.68, 2605.66
attribute OL_A bcodmo_name String DIC
attribute OL_A description String Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at optimum light (OL)
attribute OL_A long_name String OL A
attribute OL_A units String micromol/kilogram
variable OL_B   float  
attribute OL_B _FillValue float NaN
attribute OL_B actual_range float 1048.68, 2649.36
attribute OL_B bcodmo_name String DIC
attribute OL_B description String Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at optimum light (OL)
attribute OL_B long_name String OL B
attribute OL_B units String micromol/kilogram
variable EL_A   float  
attribute EL_A _FillValue float NaN
attribute EL_A actual_range float 1048.68, 2810.23
attribute EL_A bcodmo_name String DIC
attribute EL_A description String Dissolved Inorganic Carbon (DIC) measurements in replicate A incubated at extreme light (EL)
attribute EL_A long_name String EL A
attribute EL_A units String micromol/kilogram
variable EL_B   float  
attribute EL_B _FillValue float NaN
attribute EL_B actual_range float 1048.68, 2835.75
attribute EL_B bcodmo_name String DIC
attribute EL_B description String Dissolved Inorganic Carbon (DIC) measurements in replicate B incubated at extreme light (EL)
attribute EL_B long_name String EL B
attribute EL_B units String micromol/kilogram
variable EL_C   float  
attribute EL_C _FillValue float NaN
attribute EL_C actual_range float 1048.68, 2766.09
attribute EL_C bcodmo_name String DIC
attribute EL_C description String Dissolved Inorganic Carbon (DIC) measurements in replicate C incubated at extreme light (EL)
attribute EL_C long_name String EL C
attribute EL_C units String micromol/kilogram

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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