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     data   graph     files  public [PUA experimental design] - PUA (polyunsaturated aldehydes) experiments: Experimental
Conditions, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (The effects of diatom-
produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Data for experimental conditions were collected from the station locations
where water and zooplankton were collected. Temperature and salinity were
determined using a YSI ProDO2020.

Laboratory experiments were conducted by collecting raw seawater, filtering it
through 200\u00b5m mesh sieves into 20L carboys, and then dispensing it into
experimental jars. Triplicates bottles were used for each treatment.
Treatments included whole seawater (control), whole seawater plus copepods
(Zooplankton), and the same treatments plus polyunsaturated aldehydes (PUA)
additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were
dissolved in methanol and added to experimental bottles for a final
concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type
of PUA. In addition, nutrients were added to all experimental bottles in the
form of phytoplankton growth media, to ensure that autotroph growth was not
limited by available nutrients in the experimental bottles.

Samples for PUA analysis were collected and processed according to the
procedures described in Lavrentyev et al. 2015 & Franz\u00e9 et al. 2018.
These samples were then frozen and shipped to Texas for analysis.

All data were processed in Microsoft Excel.
attribute NC_GLOBAL awards_0_award_nid String 555555
attribute NC_GLOBAL awards_0_award_number String OCE-1357168
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357168 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 555561
attribute NC_GLOBAL awards_1_award_number String OCE-1357169
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357169 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String PUA (polyunsaturated aldehydes) experiments: Experimental Conditions
Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
PI's: P. Lavrentyev (U Akron), J. Pierson & D. Stoeker (UMaryland CES)
version date: 2019-07-31
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-07-31T19:36:16Z
attribute NC_GLOBAL date_modified String 2019-10-07T20:52:22Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.773979.1
attribute NC_GLOBAL Easternmost_Easting double 14.25
attribute NC_GLOBAL geospatial_lat_max double 40.808
attribute NC_GLOBAL geospatial_lat_min double 37.1656
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 14.25
attribute NC_GLOBAL geospatial_lon_min double -75.9866
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/773979 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Water Temp Sensor
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure temperature and salinity at stations.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 773990
attribute NC_GLOBAL instruments_0_description String General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/134/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Water Temperature Sensor
attribute NC_GLOBAL instruments_0_instrument_nid String 647
attribute NC_GLOBAL instruments_0_supplied_name String YSI ProDO2020
attribute NC_GLOBAL keywords String bacteria, bco, bco-dmo, biological, chemical, chl, Chl_a, chlorophyll, ciliate, copepod, Copepod_spp, copepods, cycle, dark, data, dataset, date, DecD, DecP, density, dinoflag, dmo, earth, Earth Science > Oceans > Salinity/Density > Salinity, erddap, experiment, hep, HepD, HepP, latitude, light, Light_Dark_Cycle, Location, longitude, management, medium, number, Number_Copepods, ocean, oceanography, oceans, OctD, OctP, office, practical, preliminary, salinity, science, sea, sea_water_practical_salinity, seawater, spp, temperature, time, tot, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/773979/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/773979 (external link)
attribute NC_GLOBAL Northernmost_Northing double 40.808
attribute NC_GLOBAL param_mapping String {'773979': {'Latitude': 'flag - latitude', 'Longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/773979/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Akron
attribute NC_GLOBAL people_0_affiliation_acronym String UAkron
attribute NC_GLOBAL people_0_person_name String Dr Peter Lavrentyev
attribute NC_GLOBAL people_0_person_nid String 555559
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_1_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_1_person_name String James J. Pierson
attribute NC_GLOBAL people_1_person_nid String 51109
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_2_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_2_person_name String Diane Stoecker
attribute NC_GLOBAL people_2_person_nid String 50623
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String DiatomAldehydes
attribute NC_GLOBAL projects_0_acronym String DiatomAldehydes
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
This project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes:
I. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton.
II. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton.
III. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods.
IV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA.
The experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy).
Diatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-03
attribute NC_GLOBAL projects_0_name String The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
attribute NC_GLOBAL projects_0_project_nid String 555556
attribute NC_GLOBAL projects_0_start_date String 2014-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 37.1656
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String PUA (polyunsaturated aldehydes) experiments: Experimental Conditions, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
attribute NC_GLOBAL title String [PUA experimental design] - PUA (polyunsaturated aldehydes) experiments: Experimental Conditions, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -75.9866
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment   String  
attribute Experiment bcodmo_name String exp_id
attribute Experiment description String Name of the Experiment
attribute Experiment long_name String Experiment
attribute Experiment units String unitless
variable Location   String  
attribute Location bcodmo_name String site
attribute Location description String Name of the Location
attribute Location long_name String Location
attribute Location units String unitless
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -75.9866, 14.25
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 37.1656, 40.808
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable Date   String  
attribute Date bcodmo_name String date_local
attribute Date description String Local date; formatted as yyyy-mm-dd
attribute Date long_name String Date
attribute Date source_name String Date
attribute Date time_precision String 1970-01-01
attribute Date units String unitless
variable Temperature   float  
attribute Temperature _FillValue float NaN
attribute Temperature actual_range float 6.7, 23.5
attribute Temperature bcodmo_name String temperature
attribute Temperature description String Water Temperature
attribute Temperature long_name String Temperature
attribute Temperature nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temperature units String degrees Celsius
variable Salinity   float  
attribute Salinity _FillValue float NaN
attribute Salinity actual_range float 24.9, 37.4
attribute Salinity bcodmo_name String sal
attribute Salinity colorBarMaximum double 37.0
attribute Salinity colorBarMinimum double 32.0
attribute Salinity description String Water Salinity
attribute Salinity long_name String Sea Water Practical Salinity
attribute Salinity nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute Salinity units String PSU
variable Light_Dark_Cycle   String  
attribute Light_Dark_Cycle bcodmo_name String unknown
attribute Light_Dark_Cycle description String Light:Dark Cycle used for the experiments (hours:hours)
attribute Light_Dark_Cycle long_name String Light Dark Cycle
attribute Light_Dark_Cycle units String unitless
variable Medium   String  
attribute Medium bcodmo_name String unknown
attribute Medium description String Type of medium used for added for nutrients in experiments
attribute Medium long_name String Medium
attribute Medium units String unitless
variable Copepod_spp   String  
attribute Copepod_spp bcodmo_name String taxon
attribute Copepod_spp description String Copepod species used for the experiments
attribute Copepod_spp long_name String Copepod Spp
attribute Copepod_spp units String unitless
variable Number_Copepods   byte  
attribute Number_Copepods _FillValue byte 127
attribute Number_Copepods actual_range byte 7, 15
attribute Number_Copepods bcodmo_name String count
attribute Number_Copepods colorBarMaximum double 100.0
attribute Number_Copepods colorBarMinimum double 0.0
attribute Number_Copepods description String Number of copepods added to each zooplankton treatment jar for each experiment
attribute Number_Copepods long_name String Number Copepods
attribute Number_Copepods units String copepods
variable Bacteria   float  
attribute Bacteria _FillValue float NaN
attribute Bacteria actual_range float 119000.0, 2400000.0
attribute Bacteria bcodmo_name String cell_concentration
attribute Bacteria description String Mean initial bacteria concentrations from experiments
attribute Bacteria long_name String Bacteria
attribute Bacteria units String cells per ml
variable Chl_a   String  
attribute Chl_a bcodmo_name String chlorophyll a
attribute Chl_a description String Mean initial Chlorophyll a concentrations
attribute Chl_a long_name String Chl A
attribute Chl_a nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chl_a units String micrograms/liter
variable Dinoflag   String  
attribute Dinoflag bcodmo_name String dino_het_C
attribute Dinoflag description String Mean initial Dinoflagellate biomass concentrations
attribute Dinoflag long_name String Dinoflag
attribute Dinoflag units String micrograms Carbon/liter
variable Ciliate   String  
attribute Ciliate bcodmo_name String ciliates_C
attribute Ciliate description String Mean initial Ciliate biomass concentrations
attribute Ciliate long_name String Ciliate
attribute Ciliate units String micrograms Carbon/liter
variable HepD   float  
attribute HepD _FillValue float NaN
attribute HepD actual_range float 0.0061, 0.01
attribute HepD bcodmo_name String unknown
attribute HepD description String Mean initial Dissolved Heptadienal concentrations
attribute HepD long_name String Hep D
attribute HepD units String nanoMolar
variable HepP   float  
attribute HepP _FillValue float NaN
attribute HepP actual_range float 0.014, 0.0207
attribute HepP bcodmo_name String unknown
attribute HepP description String Mean initial Particulate Heptadienal concentrations
attribute HepP long_name String Hep P
attribute HepP units String nanoMolar
variable OctD   float  
attribute OctD _FillValue float NaN
attribute OctD actual_range float 0.0043, 0.0108
attribute OctD bcodmo_name String unknown
attribute OctD description String Mean initial Dissolved Octadienal concentrations
attribute OctD long_name String Oct D
attribute OctD units String nanoMolar
variable OctP   float  
attribute OctP _FillValue float NaN
attribute OctP actual_range float 0.0082, 0.0112
attribute OctP bcodmo_name String unknown
attribute OctP description String Mean initial particulate octadienal concentrations
attribute OctP long_name String Oct P
attribute OctP units String nanoMolar
variable DecD   float  
attribute DecD _FillValue float NaN
attribute DecD actual_range float 0.0081, 0.0173
attribute DecD bcodmo_name String unknown
attribute DecD description String Mean initial Dissolved decadienal concentrations
attribute DecD long_name String Dec D
attribute DecD units String nanoMolar
variable DecP   float  
attribute DecP _FillValue float NaN
attribute DecP actual_range float 0.0071, 0.013
attribute DecP bcodmo_name String unknown
attribute DecP description String Mean initial Particulate decadienal concentrations
attribute DecP long_name String Dec P
attribute DecP units String nanoMolar
variable Tot   float  
attribute Tot _FillValue float NaN
attribute Tot actual_range float 0.049, 0.0743
attribute Tot bcodmo_name String unknown
attribute Tot description String Total mean initial PUA concentrations
attribute Tot long_name String Tot
attribute Tot units String nanoMolar

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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