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     data   graph     files  public [PUA chlorophyll a] - PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a
concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (The effects of diatom-
produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters)
   ?        I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_774017

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Experiments were conducted by collecting raw seawater, filtering it through
200\u00b5m mesh sieves into 20L carboys, and then dispensing it into
experimental jars. Triplicates bottles were used for each treatment.
Treatments included whole seawater (control), whole seawater plus copepods
(Zooplankton), and the same treatments plus PUA additions (Heptadienal,
Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and
added to experimental bottles for a final concentration of 21 nM; for the
mixed PUA treatment this was 7nM of each type of PUA.

Initial samples were collected from the carboy for chlorophyll a as described
below. Final samples were collected from each treatment and control bottle as
described below.

Chlorophyll a samples (50-150ml) were filtered onto 25mm diameter GF/F filters
(nominal 0.7 micrometer\u00a0pore size), and filters were extracted in 90%
acetone at -20 degrees C for 24 hours using the acid method (EPA Method 445 -
Arar & Collins 1997), then analyzed on a Turner Designs AU10 or TD700
fluorometer.

All data were processed in Microsoft Excel.
attribute NC_GLOBAL awards_0_award_nid String 555555
attribute NC_GLOBAL awards_0_award_number String OCE-1357168
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357168 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 555561
attribute NC_GLOBAL awards_1_award_number String OCE-1357169
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357169 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String PUA (polyunsaturated aldehydes) experiments: Chlorophyll a concentration
Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
PI's: P. Lavrentyev (U Akron), J. Pierson & D. Stoeker (UMaryland CES)
version date: 2019-07-31
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-08-01T13:47:09Z
attribute NC_GLOBAL date_modified String 2019-10-07T20:53:23Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.774017.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/774017 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Turner Fluorometer -10AU
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Chlorophyll a concentrations were determined on a Turner Designs AU10 fluorometer after extraction in 90% acetone at 0°C. The fluorometer was calibrated using a 5 point calibration with a chlorophyll a standard
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 774027
attribute NC_GLOBAL instruments_0_description String The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Turner Designs Fluorometer -10-AU
attribute NC_GLOBAL instruments_0_instrument_nid String 464
attribute NC_GLOBAL instruments_0_supplied_name String Turner Designs AU10 fluorometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, chl, chlorophyll, data, dataset, dmo, erddap, experiment, management, oceanography, office, preliminary, rep, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/774017/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/774017 (external link)
attribute NC_GLOBAL param_mapping String {'774017': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/774017/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Akron
attribute NC_GLOBAL people_0_affiliation_acronym String UAkron
attribute NC_GLOBAL people_0_person_name String Dr Peter Lavrentyev
attribute NC_GLOBAL people_0_person_nid String 555559
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_1_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_1_person_name String James J. Pierson
attribute NC_GLOBAL people_1_person_nid String 51109
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_2_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_2_person_name String Diane Stoecker
attribute NC_GLOBAL people_2_person_nid String 50623
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String DiatomAldehydes
attribute NC_GLOBAL projects_0_acronym String DiatomAldehydes
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
This project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes:
I. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton.
II. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton.
III. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods.
IV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA.
The experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy).
Diatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-03
attribute NC_GLOBAL projects_0_name String The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
attribute NC_GLOBAL projects_0_project_nid String 555556
attribute NC_GLOBAL projects_0_start_date String 2014-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015.
attribute NC_GLOBAL title String [PUA chlorophyll a] - PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment   String  
attribute Experiment bcodmo_name String exp_id
attribute Experiment description String Name of the Experiment
attribute Experiment long_name String Experiment
attribute Experiment units String unitless
variable Treatment   String  
attribute Treatment bcodmo_name String treatment
attribute Treatment description String Treatment name
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable Rep   byte  
attribute Rep _FillValue byte 127
attribute Rep actual_range byte 1, 3
attribute Rep bcodmo_name String replicate
attribute Rep description String Replicate number
attribute Rep long_name String Rep
attribute Rep units String unitless
variable Chl   float  
attribute Chl _FillValue float NaN
attribute Chl actual_range float 0.051044, 10.13012
attribute Chl bcodmo_name String chlorophyll a
attribute Chl description String Chlorophyll a concentration determined from acetone extraction
attribute Chl long_name String CHL
attribute Chl nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chl units String micrograms/liter

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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