ERDDAP > info > bcodmo_dataset_774033

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015		I   M	background			BCO-DMO	bcodmo_dataset_774033

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Experiments were conducted by collecting raw seawater, filtering it through 200\u00b5m mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus PUA additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA. Initial samples were collected from the carboy for microzooplankton as described below. Final samples were collected from each treatment and control bottle as described below. Microzooplankton samples were collected by gently decanting 100ml of each treatment bottle into a sample bottle and preserved with 2% acid Lugol\u2019s solution (final concentration). Identification and sorting of microzooplankton was done by settling 10-25 ml of sample in Uterm\u00f6hl chambers and counting with an Olympus IX-70 inverted microscope equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Microzooplankton biovolumes were calculated from their dimensions and approximate shapes (Sun and Liu 2003), and converted to carbon using published empirical relationships (Menden-Deuer and Lessard 2000). Tintinnid volumes were calculated based on their cell dimensions. All data were processed in Microsoft Excel.
attribute	NC_GLOBAL	awards_0_award_nid	String	555555
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1357168
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357168
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	awards_1_award_nid	String	555561
attribute	NC_GLOBAL	awards_1_award_number	String	OCE-1357169
attribute	NC_GLOBAL	awards_1_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357169
attribute	NC_GLOBAL	awards_1_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_1_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_1_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_1_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_1_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	PUA (polyunsaturated aldehydes) experiments: Microzooplankton Biomass Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 PI's: P. Lavrentyev (U Akron), J. Pierson & D. Stoeker (UMaryland CES) version date: 2019-09-30
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-08-01T15:21:48Z
attribute	NC_GLOBAL	date_modified	String	2019-10-07T20:53:53Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.774033.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/774033
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Inverted Microscope
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	The microscope was equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Biomass estimates for microzooplankton were determined after counting cells on an inverted microscope and converting volume estimates, from measurements with a reticle in the objective lens, to carbon concentrations using known conversion factors.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	774048
attribute	NC_GLOBAL	instruments_0_description	String	An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana). Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives. The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Inverted Microscope
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	675
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Olympus IX-70 inverted microscope
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, biomass, bottle, Bottle_ID, chemical, data, dataset, dmo, erddap, esd, experiment, management, oceanography, office, phylum, preliminary, rep, taxa, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/774033/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/774033
attribute	NC_GLOBAL	param_mapping	String	{'774033': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/774033/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Akron
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UAkron
attribute	NC_GLOBAL	people_0_person_name	String	Dr Peter Lavrentyev
attribute	NC_GLOBAL	people_0_person_nid	String	555559
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Maryland Center for Environmental Science
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UMCES/HPL
attribute	NC_GLOBAL	people_1_person_name	String	James J. Pierson
attribute	NC_GLOBAL	people_1_person_nid	String	51109
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of Maryland Center for Environmental Science
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UMCES/HPL
attribute	NC_GLOBAL	people_2_person_name	String	Diane Stoecker
attribute	NC_GLOBAL	people_2_person_nid	String	50623
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	DiatomAldehydes
attribute	NC_GLOBAL	projects_0_acronym	String	DiatomAldehydes
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: This project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes: I. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton. II. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton. III. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods. IV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA. The experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy). Diatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-03
attribute	NC_GLOBAL	projects_0_name	String	The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
attribute	NC_GLOBAL	projects_0_project_nid	String	555556
attribute	NC_GLOBAL	projects_0_start_date	String	2014-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015.
attribute	NC_GLOBAL	title	String	Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Experiment		String
attribute	Experiment	bcodmo_name	String	exp_id
attribute	Experiment	description	String	Name of the Experiment
attribute	Experiment	long_name	String	Experiment
attribute	Experiment	units	String	unitless
variable	Bottle_ID		String
attribute	Bottle_ID	bcodmo_name	String	sample
attribute	Bottle_ID	description	String	ID name for a given treatment including both treatment and replicate tags
attribute	Bottle_ID	long_name	String	Bottle ID
attribute	Bottle_ID	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Bottle_ID	units	String	unitless
variable	Treatment		String
attribute	Treatment	bcodmo_name	String	treatment
attribute	Treatment	description	String	Treatment name
attribute	Treatment	long_name	String	Treatment
attribute	Treatment	units	String	unitless
variable	Rep		byte
attribute	Rep	_FillValue	byte	127
attribute	Rep	actual_range	byte	1, 3
attribute	Rep	bcodmo_name	String	replicate
attribute	Rep	description	String	Replicate
attribute	Rep	long_name	String	Rep
attribute	Rep	units	String	unitless
variable	Taxa		String
attribute	Taxa	bcodmo_name	String	taxon
attribute	Taxa	description	String	Taxonomic name of microzooplankton type identified
attribute	Taxa	long_name	String	Taxa
attribute	Taxa	units	String	unitless
variable	Phylum		String
attribute	Phylum	bcodmo_name	String	phylum
attribute	Phylum	description	String	Phylum name for the taxa identified
attribute	Phylum	long_name	String	Phylum
attribute	Phylum	units	String	unitless
variable	ESD		byte
attribute	ESD	_FillValue	byte	127
attribute	ESD	actual_range	byte	9, 58
attribute	ESD	bcodmo_name	String	cell_size
attribute	ESD	description	String	Mean size of the taxa; given as equivalent spherical diameter (ESD)
attribute	ESD	long_name	String	ESD
attribute	ESD	units	String	micrometers
variable	Biomass		float
attribute	Biomass	_FillValue	float	NaN
attribute	Biomass	actual_range	float	0.0033, 100.9486
attribute	Biomass	bcodmo_name	String	biomass
attribute	Biomass	description	String	Biomass of the given taxa
attribute	Biomass	long_name	String	Biomass
attribute	Biomass	units	String	micrograms carbon/liter

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.