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     data   graph     files  public Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from
laboratory experiments conducted in 2016 and 2017
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_774628

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Methodology:

Duplicate cultures of Synechococcus WH8102 (axenic), Synechococcus WH7805
(xenic) and Prochlorococcus marinus MED4 (xenic and axenic) were sampled every
other day for 17-19 days for the quantification of single cells, suspended
aggregates (aggregates ca. 5-60 \u03bcm), and TEP. Single cell abundance was
determined using epifluorescence microscopy.
attribute NC_GLOBAL awards_0_award_nid String 710238
attribute NC_GLOBAL awards_0_award_number String OCE-1658527
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1658527 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Picocyanobacteria TEP: Cell Abundance
PI: Susanne Neuer
Data Version 1: 2019-08-13
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-08-06T20:50:57Z
attribute NC_GLOBAL date_modified String 2019-12-24T16:30:52Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.774628.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/774628 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String AxioScope.A1, Carl Zeiss, Germany
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 779231
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL instruments_0_supplied_name String AxioScope.A1
attribute NC_GLOBAL keywords String abundance, bco, bco-dmo, biological, cell, Cell_Abundance_rep1, Cell_Abundance_rep2, chemical, culture, data, dataset, days, dmo, erddap, management, oceanography, office, preliminary, rep1, rep2
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/774628/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/774628 (external link)
attribute NC_GLOBAL param_mapping String {'774628': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/774628/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Arizona State University
attribute NC_GLOBAL people_0_affiliation_acronym String ASU
attribute NC_GLOBAL people_0_person_name String Susanne Neuer
attribute NC_GLOBAL people_0_person_nid String 51336
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Arizona State University
attribute NC_GLOBAL people_1_affiliation_acronym String ASU
attribute NC_GLOBAL people_1_person_name String Bianca N. Cruz
attribute NC_GLOBAL people_1_person_nid String 774775
attribute NC_GLOBAL people_1_role String Student
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL people_2_affiliation String Arizona State University
attribute NC_GLOBAL people_2_affiliation_acronym String ASU
attribute NC_GLOBAL people_2_person_name String Susanne Neuer
attribute NC_GLOBAL people_2_person_nid String 51336
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Amber York
attribute NC_GLOBAL people_3_person_nid String 643627
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Marine Plankton Aggregation
attribute NC_GLOBAL projects_0_acronym String Marine Plankton Aggregation
attribute NC_GLOBAL projects_0_description String NSF abstract:
Marine phytoplankton are microscopic algae that live in the sunlit zone of the ocean. They play an important role in the uptake of carbon dioxide from the atmosphere through photosynthesis, similar to what plants do on land, and are the basis of the marine food web. However, instead of storing this organic carbon in leaf tissue and roots, marine phytoplankton are grazed by planktonic animals, or die and subsequently sink out of the sunlit zone in the form of aggregates, also called "Marine Snow". These particles not only export the organic carbon contained in their cells to the deep ocean, but also serve as food for animals and bacteria that live in the deep. A considerable portion of these phytoplankton are extremely small, among the tiniest of all organisms known. These extremely small cells have not been thought to play an important role in the formation and sinking of marine snow; however, recent findings challenge this view. This project will investigate how the smallest of these phytoplankton contribute to the rain of sinking particles from the sunlit surface to the deep ocean. This research is important because, in some of the largest expanses of the open oceans, these minute cells dominate the phytoplankton community, and larger plankton organisms are very sparse. The project, through a combination of work in the laboratory and at a field station, will shed light on how these tiny phytoplankton cells make aggregates, which ultimately enable them to sink as "Marine Snow". The project also provides unique opportunities for undergraduate students at Arizona State University, a land-locked public university, to gain experience in working with marine research. The project will serve to educate one PhD student, one MS student in an accelerated BS-MS program, and 8-10 undergraduate students/semester in a unique, inquiry based learning effort termed Microbial EducatioN Training and OutReach (MENTOR). The undergraduate students will also participate in Arizona State University (ASU)'s School of Life Sciences, Undergraduate Research Program (SOLUR), which seeks to increase the participation of minorities in science. They will also contribute towards developing web and classroom materials, based on this project, which will then be distributed through a partnership with the award-winning ASU-sponsored Ask A Biologist K-12 web site.
The oceanic "biological carbon pump", the photosynthetically mediated transformation of dissolved inorganic carbon into particulate and dissolved organic carbon and its subsequent export to deep water, functions as a significant driver of atmospheric carbon uptake by the oceans. The traditional view of the biological carbon pump in the ocean is that of sinking of large aggregates (marine snow) or fecal pellets, which are made up of large, mineral ballasted cells of phytoplankton. However, recent evidence, stemming from in situ investigations of particulate matter, trap studies and modelling studies, have shown that micron-sized phytoplankton such as picocyanobacteria as well as picoeukaryotes can contribute significantly to the sinking of particulate matter. The specific mechanisms behind the sinking of these micrometer sized cells remain elusive as the cells are too small to sink on their own, and mesozooplankton is likely unable to ingest single cells. Intriguingly, recent research by the investigators has shown that the ubiquitous picocyanobacteria Synechococcus are able to form aggregates and sink at velocities comparable to those of marine snow. They found that the matrix of the Synechococcus aggregates was made of Transparent Exopolymeric Particles (TEP), and that TEP production was enhanced under nutrient limited culture conditions. Interaction with clays and presence of heterotrophic bacteria also enhanced aggregation and sinking velocity. This study aims to further investigate aggregation of other common picoplankton in the laboratory and aggregation occurring in natural settings at an oligotrophic open ocean site, the Bermuda Atlantic Time-series Site (BATS). Ultimately, this project will increase and refine our understanding of the role of the smallest phytoplankton in aggregation and sinking - information vital to understanding carbon cycling processes in the oceans.
attribute NC_GLOBAL projects_0_end_date String 2020-02
attribute NC_GLOBAL projects_0_geolocation String Bermuda Atlantic Time-Series station
attribute NC_GLOBAL projects_0_name String Aggregation of Marine Picoplankton
attribute NC_GLOBAL projects_0_project_nid String 710239
attribute NC_GLOBAL projects_0_start_date String 2017-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017.
attribute NC_GLOBAL title String Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Culture   String  
attribute Culture bcodmo_name String sample_descrip
attribute Culture description String Culture name
attribute Culture long_name String Culture
attribute Culture units String unitless
variable Days   byte  
attribute Days _FillValue byte 127
attribute Days actual_range byte 1, 19
attribute Days bcodmo_name String time_elapsed
attribute Days description String Days since experiment start
attribute Days long_name String Days
attribute Days nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute Days units String count (days)
variable Cell_Abundance_rep1   double  
attribute Cell_Abundance_rep1 _FillValue double NaN
attribute Cell_Abundance_rep1 actual_range double 408070.74, 1.828156904E8
attribute Cell_Abundance_rep1 bcodmo_name String abundance
attribute Cell_Abundance_rep1 description String Cell abundance as determined using epifluorescence microscopy for replicate 1.
attribute Cell_Abundance_rep1 long_name String Cell Abundance Rep1
attribute Cell_Abundance_rep1 nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Cell_Abundance_rep1 units String cells per mL
variable Cell_Abundance_rep2   double  
attribute Cell_Abundance_rep2 _FillValue double NaN
attribute Cell_Abundance_rep2 actual_range double 599863.98, 1.836318319E8
attribute Cell_Abundance_rep2 bcodmo_name String abundance
attribute Cell_Abundance_rep2 description String Cell abundance as determined using epifluorescence microscopy for replicate 2.
attribute Cell_Abundance_rep2 long_name String Cell Abundance Rep2
attribute Cell_Abundance_rep2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Cell_Abundance_rep2 units String cells per mL

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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