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     data   graph     files  public Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry    ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_778021

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String We determined that the most reliable way to prepare dissolved 15N2 gas
standards involves equilibration of the liquid phase with a headspace, rather
than injection of a 15N2 gas aliquot into a sealed bottle without a headspace.
Standards were prepared in a room with a relatively constant ambient
temperature. Serial aliquots of 15N2 gas were each injected into the headspace
of crimp-sealed 120 mL serum bottles containing 80 mL of air-equilibrated
seawater (at room temperature) and a stir bar with an unpressurized air
headspace. The vials were equilibrated for 72 hours on a stir plate (at low
rpm) prior to analysis on the MIMS or IRMS.\u00a0
attribute NC_GLOBAL awards_0_award_nid String 772538
attribute NC_GLOBAL awards_0_award_number String OCE-1732246
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1732246 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Preparation of dissolved 15N2 standards for MIMS and/or IRMS
PI: Julie Granger
Version: 2019-09-30
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-09-30T13:37:38Z
attribute NC_GLOBAL date_modified String 2019-10-02T18:47:29Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.778021.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/778021 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String continuous flow Delta V Isotope Ratio Mass Spectrometer (Smith et al. 2015), and�continuous flow-GV Isoprime IRMS (Charoenpong et al., 2014)
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 778029
attribute NC_GLOBAL instruments_0_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 469
attribute NC_GLOBAL instruments_0_supplied_name String Isotope Ratio Mass Spectrometer
attribute NC_GLOBAL instruments_1_acronym String MIMS
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Membrane Inlet Mass Spectrometer (Bay Instruments)
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 778028
attribute NC_GLOBAL instruments_1_description String Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.
attribute NC_GLOBAL instruments_1_instrument_name String Membrane Inlet Mass Spectrometer
attribute NC_GLOBAL instruments_1_instrument_nid String 661606
attribute NC_GLOBAL instruments_1_supplied_name String Membrane Inlet Mass Spectrometer
attribute NC_GLOBAL keywords String analysis, At_15N_pcnt, At_15N_pcnt_avg, average, bco, bco-dmo, biological, bottle, Bottle_vol, chemical, data, dataset, dmo, erddap, expected, Expected_15N_At_pcnt, injection, Injection_15N2, m_z_28, m_z_29, m_z_30, m_z_32, m_z_40, management, N2_Ar, oceanography, office, pcnt, preliminary, ratio, ratio_28_29, ratio_28_30, sample, time, Time_of_analysis, vol
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/778021/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/778021 (external link)
attribute NC_GLOBAL param_mapping String {'778021': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/778021/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Connecticut
attribute NC_GLOBAL people_0_affiliation_acronym String UConn
attribute NC_GLOBAL people_0_person_name String Julie Granger
attribute NC_GLOBAL people_0_person_nid String 528937
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Massachusetts Dartmouth
attribute NC_GLOBAL people_1_affiliation_acronym String UMass Dartmouth
attribute NC_GLOBAL people_1_person_name String Annie Bourbonnais
attribute NC_GLOBAL people_1_person_nid String 778011
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Hawaii
attribute NC_GLOBAL people_2_person_name String Samuel Wilson
attribute NC_GLOBAL people_2_person_nid String 51733
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Mathew Biddle
attribute NC_GLOBAL people_3_person_nid String 708682
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String EAGER NitFix
attribute NC_GLOBAL projects_0_acronym String EAGER NitFix
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:
The availability of nitrogen is required to support the growth and production of organisms living in the surface of our global ocean. This element can be scarce. To alleviate this scarcity, a special class of bacteria and archaea, called nitrogen fixers, can derive the nitrogen needed for growth from nitrogen gas. This project would carefully examine one specific method for measuring nitrogen fixation that has been used recently to suggest the occurrence of small amounts of nitrogen fixation in subsurface ocean waters. If these reports are verified, then a revision of our understanding of the marine nitrogen cycle may be needed. The Ocean Carbon and Biogeochemistry program will be used as a platform to develop community consensus for best practices in nitrogen fixation measurements and detection of diversity, activity, and abundances of the organisms responsible. In addition, a session will be organized in a future national/international conference to communicate with the broader scientific community while developing these best practices.
The goal of this study is to conduct a thorough examination of potential experimental and analytical errors inherent to the 15N2-tracer nitrogen fixation method, in tandem with comprehensive molecular measurements, in mesopelagic ocean waters. Samples will be collected and experimental work conducted on a cruise transect in the North Atlantic Ocean, followed by analytical work in the laboratory. The specific aims of this study are to (1) determine the minimum quantifiable rates of 15N2 fixation based on incubations of mesopelagic waters via characterization of sources of experimental and analytical error, and (2) seek evidence of presence and expression of nitrogen fixation genes via comprehensive molecular approaches on corresponding samples. The range of detectable rates and diazotroph activity from the measurements made in this study will be informative for the understanding of the importance of nitrogen fixation in the oceanic nitrogen budget.
attribute NC_GLOBAL projects_0_end_date String 2018-10
attribute NC_GLOBAL projects_0_geolocation String North Atlantic Ocean, Pacific Ocean
attribute NC_GLOBAL projects_0_name String EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean
attribute NC_GLOBAL projects_0_project_nid String 772534
attribute NC_GLOBAL projects_0_start_date String 2017-05
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String In order to verify that measurements of the 15N2 atom% of dissolved N2 gas samples effectuated by Membrane Inlet Mass Spectrometry are accurate, we developed a specific protocol to prepare dissolved 15N2 gas standards. We detail the protocol herein, and report representative MIMS measurements of the prepared standards.
attribute NC_GLOBAL title String Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Bottle_vol   float  
attribute Bottle_vol _FillValue float NaN
attribute Bottle_vol actual_range float 80.0, 84.66
attribute Bottle_vol bcodmo_name String unknown
attribute Bottle_vol description String volume of the bottle
attribute Bottle_vol long_name String Bottle Vol
attribute Bottle_vol units String mililiters (mL)
variable Sample   String  
attribute Sample bcodmo_name String unknown
attribute Sample description String type of sample
attribute Sample long_name String Sample
attribute Sample units String unitless
variable Injection_15N2   String  
attribute Injection_15N2 bcodmo_name String unknown
attribute Injection_15N2 description String amount of Injection of 15N2
attribute Injection_15N2 long_name String Injection 15 N2
attribute Injection_15N2 units String mililiters (mL)
variable Expected_15N_At_pcnt   float  
attribute Expected_15N_At_pcnt _FillValue float NaN
attribute Expected_15N_At_pcnt actual_range float 0.131769, 12.5
attribute Expected_15N_At_pcnt bcodmo_name String unknown
attribute Expected_15N_At_pcnt description String expected 15N At %
attribute Expected_15N_At_pcnt long_name String Expected 15 N At Pcnt
attribute Expected_15N_At_pcnt units String unitless
variable Time_of_analysis   String  
attribute Time_of_analysis bcodmo_name String time
attribute Time_of_analysis description String time of analysis in 24 hour format
attribute Time_of_analysis long_name String Time Of Analysis
attribute Time_of_analysis nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Time_of_analysis units String unitless
variable m_z_28   float  
attribute m_z_28 _FillValue float NaN
attribute m_z_28 actual_range float 9.475235E-9, 0.37
attribute m_z_28 bcodmo_name String unknown
attribute m_z_28 description String mass-to-charge
attribute m_z_28 long_name String M Z 28
attribute m_z_28 units String unitless
variable m_z_29   float  
attribute m_z_29 _FillValue float NaN
attribute m_z_29 actual_range float 6.98E-11, 0.43
attribute m_z_29 bcodmo_name String unknown
attribute m_z_29 description String mass-to-charge
attribute m_z_29 long_name String M Z 29
attribute m_z_29 units String unitless
variable m_z_30   float  
attribute m_z_30 _FillValue float NaN
attribute m_z_30 actual_range float 8.277643E-13, 0.47
attribute m_z_30 bcodmo_name String unknown
attribute m_z_30 description String mass-to-charge
attribute m_z_30 long_name String M Z 30
attribute m_z_30 units String unitless
variable m_z_32   float  
attribute m_z_32 _FillValue float NaN
attribute m_z_32 actual_range float 4.538616E-12, 0.38
attribute m_z_32 bcodmo_name String unknown
attribute m_z_32 description String mass-to-charge
attribute m_z_32 long_name String M Z 32
attribute m_z_32 units String unitless
variable m_z_40   float  
attribute m_z_40 _FillValue float NaN
attribute m_z_40 actual_range float 2.412238E-10, 0.32
attribute m_z_40 bcodmo_name String unknown
attribute m_z_40 description String mass-to-charge
attribute m_z_40 long_name String M Z 40
attribute m_z_40 units String unitless
variable N2_Ar   float  
attribute N2_Ar _FillValue float NaN
attribute N2_Ar actual_range float 0.05, 39.36209
attribute N2_Ar bcodmo_name String unknown
attribute N2_Ar description String N2/Ar ratio
attribute N2_Ar long_name String N2 Ar
attribute N2_Ar units String unitless
variable ratio_28_29   float  
attribute ratio_28_29 _FillValue float NaN
attribute ratio_28_29 actual_range float 129.589, 136.4494
attribute ratio_28_29 bcodmo_name String unknown
attribute ratio_28_29 description String 28/29 ratio
attribute ratio_28_29 long_name String Ratio 28 29
attribute ratio_28_29 units String unitless
variable ratio_28_30   float  
attribute ratio_28_30 _FillValue float NaN
attribute ratio_28_30 actual_range float 7.264303, 11540.25
attribute ratio_28_30 bcodmo_name String unknown
attribute ratio_28_30 description String 28/30 ratio
attribute ratio_28_30 long_name String Ratio 28 30
attribute ratio_28_30 units String unitless
variable At_15N_pcnt   double  
attribute At_15N_pcnt _FillValue double NaN
attribute At_15N_pcnt actual_range double 0.372353, 12.355571234720752
attribute At_15N_pcnt bcodmo_name String unknown
attribute At_15N_pcnt description String 15N At%
attribute At_15N_pcnt long_name String At 15 N Pcnt
attribute At_15N_pcnt units String unitless
variable At_15N_pcnt_avg   double  
attribute At_15N_pcnt_avg _FillValue double NaN
attribute At_15N_pcnt_avg actual_range double 0.3724829634765998, 12.353433924138663
attribute At_15N_pcnt_avg bcodmo_name String unknown
attribute At_15N_pcnt_avg description String 15N At% average
attribute At_15N_pcnt_avg long_name String At 15 N Pcnt Avg
attribute At_15N_pcnt_avg units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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