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Grid DAP Data | Sub- set | Table DAP Data | Make A Graph | W M S | Source Data Files | Acces- sible | Title | Sum- mary | FGDC, ISO, Metadata | Back- ground Info | RSS | E | Institution | Dataset ID |
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data | graph | files | public | [trial_a] - Trial A test of the dissolution method for estimates of the 15N2 atom% of incubations (EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean) | I M | background | BCO-DMO | bcodmo_dataset_778126 |
Row Type | Variable Name | Attribute Name | Data Type | Value |
---|---|---|---|---|
attribute | NC_GLOBAL | access_formats | String | .htmlTable,.csv,.json,.mat,.nc,.tsv |
attribute | NC_GLOBAL | acquisition_description | String | Inocula of 15N2-enriched water were prepared according to either of two protocols outlined by Klawonn et al. (2015).\u00a0 In a first Trial A, respective 1.9 mL of 15N2 gas aliquots (Cambridge Isotope Laboratories, Lot #I-21065) were injected into crimped-sealed 120 mL glass serum vials filled with deionized water. To dissolve the 15N2 bubble, each of the two serum vials was vortexed for 5 minutes. Two subsamples of each inoculum were dispensed into Exetainers\u2122 with a peristaltic pump for analysis on the MIMS. An aliquot of each inoculum (5 % vol/vol) was then dispensed in replicate 160 mL serum incubation bottles containing air- equilibrated deionized water (Trials A1-A4), which were then crimped-sealed. Following homogenization, triplicate subsamples of each incubation were collected in Exetainers\u2122 for MIMS analysis. The 15N atom % of the inocula and of the corresponding incubations were measured by MIMS at the University of Connecticut (Bay Instruments) and computed as follows:\u00a0 Equation 4:\u00a0 In both trials, the concentration of N2 isotopologues (m/z 28, 29, and 30) in each of the 15N2-enriched inocula was then extrapolated from the ionization efficiency of N isotopologues in air-equilibrated seawater. We define the ionization efficiency as the ratio of the isotopologue ion current measured by MIMS relative to its concentration in air-equilibrated seawater (ASW):\u00a0 \u00a0 Equation S2:\u00a0 For instance, at a temperature of 25\u00baC and salinity of 35 psu, the solubility coefficients of Hamme and Emerson (2004) predict a N2 concentration of 388.9 \u03bcmol kg-1. The fraction of 15N in N2 (i.e., 15N/(14N+15N)) for air-equilibrated seawater is 0.003663 (Mariotti, 1983), such that the expected fractions of 28N2, 29N2, and 30N2 derived from their binomial probability distributions are as follows: \u00a0 \u00a0 = 99.2687 % Equation S3a = 0.7299 % Equation S3b = 0.0013 % Equation S3c \u00a0 Accordingly, air-equilibrated concentrations of 28N2, 29N2, and 30N2 at this temperature and salinity are 386.0, 2.8, and 0.005 \u03bcmol kg-1, respectively. The ionization efficiency of the isotopologues is then equal to the ion current of m/z 28 recorded for ASW divided by the corresponding 28N2 concentration (Eq. S2). We used the ionization efficiency of m/z 28 in ASW to derive the N2 isotopologue concentrations in the inocula from their respective MIMS ion currents. We did not derive distinct ionization efficiencies from the ion current-to-concentration of m/z 29 and 30 in ASW, as these isotopologues are poorly resolved by the MIMS at natural abundance. Thus, we are assuming that the ionization efficiency of m/z 29 and 30 isotopologues is roughly similar to that of m/z 28 (i.e., that ionization isotope effects are negligible for our purposes). The initial expected AN2 of the \u201cincubations\u201d was then calculated using a linear mixing model with N2 isotopologue concentrations in ambient and enriched seawater |
attribute | NC_GLOBAL | awards_0_award_nid | String | 772538 |
attribute | NC_GLOBAL | awards_0_award_number | String | OCE-1732246 |
attribute | NC_GLOBAL | awards_0_data_url | String | http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1732246 |
attribute | NC_GLOBAL | awards_0_funder_name | String | NSF Division of Ocean Sciences |
attribute | NC_GLOBAL | awards_0_funding_acronym | String | NSF OCE |
attribute | NC_GLOBAL | awards_0_funding_source_nid | String | 355 |
attribute | NC_GLOBAL | awards_0_program_manager | String | Henrietta N Edmonds |
attribute | NC_GLOBAL | awards_0_program_manager_nid | String | 51517 |
attribute | NC_GLOBAL | cdm_data_type | String | Other |
attribute | NC_GLOBAL | comment | String | Trial A test of the dissolution method PI: Julie Granger Version: 2019-09-30 |
attribute | NC_GLOBAL | Conventions | String | COARDS, CF-1.6, ACDD-1.3 |
attribute | NC_GLOBAL | creator_email | String | info at bco-dmo.org |
attribute | NC_GLOBAL | creator_name | String | BCO-DMO |
attribute | NC_GLOBAL | creator_type | String | institution |
attribute | NC_GLOBAL | creator_url | String | https://www.bco-dmo.org/ |
attribute | NC_GLOBAL | data_source | String | extract_data_as_tsv version 2.3 19 Dec 2019 |
attribute | NC_GLOBAL | date_created | String | 2019-09-30T19:01:20Z |
attribute | NC_GLOBAL | date_modified | String | 2019-10-02T18:51:48Z |
attribute | NC_GLOBAL | defaultDataQuery | String | &time<now |
attribute | NC_GLOBAL | doi | String | 10.1575/1912/bco-dmo.778126.1 |
attribute | NC_GLOBAL | infoUrl | String | https://www.bco-dmo.org/dataset/778126 |
attribute | NC_GLOBAL | institution | String | BCO-DMO |
attribute | NC_GLOBAL | instruments_0_acronym | String | IR Mass Spec |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_description | String | continuous flow Delta V Isotope Ratio Mass Spectrometer (Smith et al. 2015), and continuous flow-GV Isoprime IRMS (Charoenpong et al., 2014) |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_nid | String | 778134 |
attribute | NC_GLOBAL | instruments_0_description | String | The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). |
attribute | NC_GLOBAL | instruments_0_instrument_external_identifier | String | https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ |
attribute | NC_GLOBAL | instruments_0_instrument_name | String | Isotope-ratio Mass Spectrometer |
attribute | NC_GLOBAL | instruments_0_instrument_nid | String | 469 |
attribute | NC_GLOBAL | instruments_0_supplied_name | String | Isotope Ratio Mass Spectrometer |
attribute | NC_GLOBAL | instruments_1_acronym | String | MIMS |
attribute | NC_GLOBAL | instruments_1_dataset_instrument_description | String | Membrane Inlet Mass Spectrometer (Bay Instruments) |
attribute | NC_GLOBAL | instruments_1_dataset_instrument_nid | String | 778133 |
attribute | NC_GLOBAL | instruments_1_description | String | Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane. |
attribute | NC_GLOBAL | instruments_1_instrument_name | String | Membrane Inlet Mass Spectrometer |
attribute | NC_GLOBAL | instruments_1_instrument_nid | String | 661606 |
attribute | NC_GLOBAL | instruments_1_supplied_name | String | Membrane Inlet Mass Spectrometer |
attribute | NC_GLOBAL | keywords | String | analysis, average, avg_measured_a_pcnt, baro, Baro_Press, bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, m_z_28, m_z_29, m_z_30, m_z_32, m_z_40, management, meas, meas_at_pcnt, measured, N2_Ar, oceanography, office, pcnt, preliminary, press, ratio, ratio_28_29, ratio_28_30, sample, time, Time_of_analysis |
attribute | NC_GLOBAL | license | String | https://www.bco-dmo.org/dataset/778126/license |
attribute | NC_GLOBAL | metadata_source | String | https://www.bco-dmo.org/api/dataset/778126 |
attribute | NC_GLOBAL | param_mapping | String | {'778126': {}} |
attribute | NC_GLOBAL | parameter_source | String | https://www.bco-dmo.org/mapserver/dataset/778126/parameters |
attribute | NC_GLOBAL | people_0_affiliation | String | University of Connecticut |
attribute | NC_GLOBAL | people_0_affiliation_acronym | String | UConn |
attribute | NC_GLOBAL | people_0_person_name | String | Julie Granger |
attribute | NC_GLOBAL | people_0_person_nid | String | 528937 |
attribute | NC_GLOBAL | people_0_role | String | Principal Investigator |
attribute | NC_GLOBAL | people_0_role_type | String | originator |
attribute | NC_GLOBAL | people_1_affiliation | String | University of Massachusetts Dartmouth |
attribute | NC_GLOBAL | people_1_affiliation_acronym | String | UMass Dartmouth |
attribute | NC_GLOBAL | people_1_person_name | String | Annie Bourbonnais |
attribute | NC_GLOBAL | people_1_person_nid | String | 778011 |
attribute | NC_GLOBAL | people_1_role | String | Co-Principal Investigator |
attribute | NC_GLOBAL | people_1_role_type | String | originator |
attribute | NC_GLOBAL | people_2_affiliation | String | University of Hawaii |
attribute | NC_GLOBAL | people_2_person_name | String | Samuel Wilson |
attribute | NC_GLOBAL | people_2_person_nid | String | 51733 |
attribute | NC_GLOBAL | people_2_role | String | Co-Principal Investigator |
attribute | NC_GLOBAL | people_2_role_type | String | originator |
attribute | NC_GLOBAL | people_3_affiliation | String | Woods Hole Oceanographic Institution |
attribute | NC_GLOBAL | people_3_affiliation_acronym | String | WHOI BCO-DMO |
attribute | NC_GLOBAL | people_3_person_name | String | Mathew Biddle |
attribute | NC_GLOBAL | people_3_person_nid | String | 708682 |
attribute | NC_GLOBAL | people_3_role | String | BCO-DMO Data Manager |
attribute | NC_GLOBAL | people_3_role_type | String | related |
attribute | NC_GLOBAL | project | String | EAGER NitFix |
attribute | NC_GLOBAL | projects_0_acronym | String | EAGER NitFix |
attribute | NC_GLOBAL | projects_0_description | String | NSF Award Abstract: The availability of nitrogen is required to support the growth and production of organisms living in the surface of our global ocean. This element can be scarce. To alleviate this scarcity, a special class of bacteria and archaea, called nitrogen fixers, can derive the nitrogen needed for growth from nitrogen gas. This project would carefully examine one specific method for measuring nitrogen fixation that has been used recently to suggest the occurrence of small amounts of nitrogen fixation in subsurface ocean waters. If these reports are verified, then a revision of our understanding of the marine nitrogen cycle may be needed. The Ocean Carbon and Biogeochemistry program will be used as a platform to develop community consensus for best practices in nitrogen fixation measurements and detection of diversity, activity, and abundances of the organisms responsible. In addition, a session will be organized in a future national/international conference to communicate with the broader scientific community while developing these best practices. The goal of this study is to conduct a thorough examination of potential experimental and analytical errors inherent to the 15N2-tracer nitrogen fixation method, in tandem with comprehensive molecular measurements, in mesopelagic ocean waters. Samples will be collected and experimental work conducted on a cruise transect in the North Atlantic Ocean, followed by analytical work in the laboratory. The specific aims of this study are to (1) determine the minimum quantifiable rates of 15N2 fixation based on incubations of mesopelagic waters via characterization of sources of experimental and analytical error, and (2) seek evidence of presence and expression of nitrogen fixation genes via comprehensive molecular approaches on corresponding samples. The range of detectable rates and diazotroph activity from the measurements made in this study will be informative for the understanding of the importance of nitrogen fixation in the oceanic nitrogen budget. |
attribute | NC_GLOBAL | projects_0_end_date | String | 2018-10 |
attribute | NC_GLOBAL | projects_0_geolocation | String | North Atlantic Ocean, Pacific Ocean |
attribute | NC_GLOBAL | projects_0_name | String | EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean |
attribute | NC_GLOBAL | projects_0_project_nid | String | 772534 |
attribute | NC_GLOBAL | projects_0_start_date | String | 2017-05 |
attribute | NC_GLOBAL | publisher_name | String | Biological and Chemical Oceanographic Data Management Office (BCO-DMO) |
attribute | NC_GLOBAL | publisher_type | String | institution |
attribute | NC_GLOBAL | sourceUrl | String | (local files) |
attribute | NC_GLOBAL | standard_name_vocabulary | String | CF Standard Name Table v55 |
attribute | NC_GLOBAL | summary | String | The \u201cdissolution\u201d method to measure N2 fixation rates with 15N2 gas tracer involves the preparation of 15N2-enriched water that is then added to each incubation bottle. Investigators typically measure the 15N2 atom% of the 15N2-enriched inoculum by MIMS, and extrapolate the 15N2 atom% in the incubations based on the inoculum value. Here, we demonstrate that such extrapolation yields inaccurate estimates of the 15N2 atom% of incubations. The latter should be measured directly. |
attribute | NC_GLOBAL | title | String | [trial_a] - Trial A test of the dissolution method for estimates of the 15N2 atom% of incubations (EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean) |
attribute | NC_GLOBAL | version | String | 1 |
attribute | NC_GLOBAL | xml_source | String | osprey2erddap.update_xml() v1.3 |
variable | Sample | String | ||
attribute | Sample | bcodmo_name | String | sample |
attribute | Sample | description | String | sample |
attribute | Sample | long_name | String | Sample |
attribute | Sample | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ |
attribute | Sample | units | String | unitless |
variable | Baro_Press | float | ||
attribute | Baro_Press | _FillValue | float | NaN |
attribute | Baro_Press | actual_range | float | 30.51, 30.53 |
attribute | Baro_Press | bcodmo_name | String | unknown |
attribute | Baro_Press | description | String | barometric pressue |
attribute | Baro_Press | long_name | String | Baro Press |
attribute | Baro_Press | units | String | unknown |
variable | Time_of_analysis | String | ||
attribute | Time_of_analysis | bcodmo_name | String | time |
attribute | Time_of_analysis | description | String | time of analysis |
attribute | Time_of_analysis | long_name | String | Time Of Analysis |
attribute | Time_of_analysis | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ |
attribute | Time_of_analysis | units | String | unitless |
variable | m_z_28 | double | ||
attribute | m_z_28 | _FillValue | double | NaN |
attribute | m_z_28 | actual_range | double | 1.946714E-8, 0.16527391698923202 |
attribute | m_z_28 | bcodmo_name | String | unknown |
attribute | m_z_28 | description | String | mass-to-charge |
attribute | m_z_28 | long_name | String | M Z 28 |
attribute | m_z_28 | units | String | unitless |
variable | m_z_29 | double | ||
attribute | m_z_29 | _FillValue | double | NaN |
attribute | m_z_29 | actual_range | double | 1.591554E-10, 0.13304737362866276 |
attribute | m_z_29 | bcodmo_name | String | unknown |
attribute | m_z_29 | description | String | mass-to-charge |
attribute | m_z_29 | long_name | String | M Z 29 |
attribute | m_z_29 | units | String | unitless |
variable | m_z_30 | double | ||
attribute | m_z_30 | _FillValue | double | NaN |
attribute | m_z_30 | actual_range | double | 3.340442E-12, 3.080166212540741 |
attribute | m_z_30 | bcodmo_name | String | unknown |
attribute | m_z_30 | description | String | mass-to-charge |
attribute | m_z_30 | long_name | String | M Z 30 |
attribute | m_z_30 | units | String | unitless |
variable | m_z_32 | double | ||
attribute | m_z_32 | _FillValue | double | NaN |
attribute | m_z_32 | actual_range | double | 1.735591E-11, 1.062805216597332 |
attribute | m_z_32 | bcodmo_name | String | unknown |
attribute | m_z_32 | description | String | mass-to-charge |
attribute | m_z_32 | long_name | String | M Z 32 |
attribute | m_z_32 | units | String | unitless |
variable | m_z_40 | double | ||
attribute | m_z_40 | _FillValue | double | NaN |
attribute | m_z_40 | actual_range | double | 5.645087E-10, 1.1222807152753036 |
attribute | m_z_40 | bcodmo_name | String | unknown |
attribute | m_z_40 | description | String | mass-to-charge |
attribute | m_z_40 | long_name | String | M Z 40 |
attribute | m_z_40 | units | String | unitless |
variable | N2_Ar | float | ||
attribute | N2_Ar | _FillValue | float | NaN |
attribute | N2_Ar | actual_range | float | 33.95307, 39.72807 |
attribute | N2_Ar | bcodmo_name | String | unknown |
attribute | N2_Ar | description | String | N2/Ar ratio |
attribute | N2_Ar | long_name | String | N2 Ar |
attribute | N2_Ar | units | String | unitless |
variable | ratio_28_29 | float | ||
attribute | ratio_28_29 | _FillValue | float | NaN |
attribute | ratio_28_29 | actual_range | float | 120.9205, 135.7853 |
attribute | ratio_28_29 | bcodmo_name | String | unknown |
attribute | ratio_28_29 | description | String | 28/29 ratio |
attribute | ratio_28_29 | long_name | String | Ratio 28 29 |
attribute | ratio_28_29 | units | String | unitless |
variable | ratio_28_30 | float | ||
attribute | ratio_28_30 | _FillValue | float | NaN |
attribute | ratio_28_30 | actual_range | float | 1.430992, 10582.37 |
attribute | ratio_28_30 | bcodmo_name | String | unknown |
attribute | ratio_28_30 | description | String | 28/30 ratio |
attribute | ratio_28_30 | long_name | String | Ratio 28 30 |
attribute | ratio_28_30 | units | String | unitless |
variable | meas_at_pcnt | double | ||
attribute | meas_at_pcnt | _FillValue | double | NaN |
attribute | meas_at_pcnt | actual_range | double | 0.3756647104406213, 41.17840652185543 |
attribute | meas_at_pcnt | bcodmo_name | String | unknown |
attribute | meas_at_pcnt | description | String | measured atom percent |
attribute | meas_at_pcnt | long_name | String | Meas At Pcnt |
attribute | meas_at_pcnt | units | String | unitless |
variable | avg_measured_a_pcnt | float | ||
attribute | avg_measured_a_pcnt | _FillValue | float | NaN |
attribute | avg_measured_a_pcnt | actual_range | float | 0.38, 41.11 |
attribute | avg_measured_a_pcnt | bcodmo_name | String | unknown |
attribute | avg_measured_a_pcnt | description | String | average measured atom percent |
attribute | avg_measured_a_pcnt | long_name | String | Avg Measured A Pcnt |
attribute | avg_measured_a_pcnt | units | String | unitless |
The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.