ERDDAP > info > bcodmo_dataset_778426

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[Final spores] - Pterosiphonia bipinnata and Corallina vancouveriensis final experiment shear and percent remaining at Deadman Bay on San Juan Island, Washington (Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective)		F   I   M	background			BCO-DMO	bcodmo_dataset_778426

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	Sporophytic specimens of Pterosiphonia bipinnata and Corallina vancouveriensis were collected from the mid to low intertidal zone on San Juan Island, Washington (Deadman Bay: 48 30' 48.93\"N, 123 8'56.14\"W), immediately transported to Friday Harbor Laboratories (FHL), and maintained in an outdoor seawater table for up to one week before testing. Reproductive parent fronds were held at ambient levels of seawater pH/pCO2 and were only exposed to experimental conditions while releasing spores. Spore release and settlement was performed in the Ocean Acidification Environmental Laboratory (OAEL) at FHL, allowing precise control of pH and temperature in a flow-through system. Two pH treatments were established by bubbling CO2 (7.75, 7.30 total scale) at 11C and confirmed with carbonate water chemistry analyses. Specifically, spectrophotometric pH was determined as per SOP6 and total alkalinity was measured using an open cell titrator as outlined in SOP3b (Dickson et al. 2007). Ambient pH values for the Salish Sea are approximately 7.8, and pH as low as 7.3 has been documented in nearshore environments in Washington.\u00a0 \u00a0The spore settlement apparatus consisted of a carriage system that slowly (1-2.5 cm/hr.) drove reproductive algal thalli across a glass settlement plate (0.6 x 7.6 x 60 cm), while releasing spores. The working section (14 x 1.5 cm) was defined at one end of the plate. Spores landing in the working section had a decreasing gradient of attachment time, determined by the rate parent thalli were driven across the plate and the time the plate was allowed to set. Error in attachment time was estimated to be 6-12 minutes given sinking rates of spores of comparable spore size (50-100 um) and release height (5-10 cm). The shear flume was designed to release a tall column of water that flushes quickly across the working section of the spore settlement plate. Water column height was varied to create a range of shear stresses. \u00a0Shear stresses generated by each water column height were calculated according to Schultz et al. 2000, using the height of the channel (4.3 mm), the pressure gradient across the working section (measured with a manometer), and the length of the working section. \u00a0Most spores were removed in the first 10 seconds of continuous exposure to shear and longer exposure times result in little additional detachment, so trials were limited to 15 seconds.\u00a0 Prior to each test, the shear flume was fitted on the settlement plate with clamps and the released spores were photographed using a microscope (Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix S3300), using the lines drawn in the working section for reference. For the attachment time assay, a low shear stress (1 Pa) was applied and the remaining spores were photographed and counted.\u00a0 For the attachment strength assay, spores were allowed to set in stationary water for 35-48 hours in P. bipinnata and for 5-10 hours in C. vancouveriensis, since maximum attachment of each species was found in these time frames using the logistic regression analyses of attachment time. For P. bipinnata, replication was achieved by repeating the experiment in time since one frond moved across the entire settlement plate in each run. Due to limitations with C. vancouveriensis spore release, several fronds were used to provide sufficient spores for this assay. In this case, each frond that released spores was considered a replicate, and this was repeated across several days. All spore releases were natural, as attempts to artificially induce spore release (osmotic, temperature, or light) were unsuccessful.\u00a0 Attached spores were exposed to increasing shear stresses (1, 4, 7, 17 and 20 Pa). Shear stresses in the flume represented boundary layer velocities of 0.2 \u2013 4 ms-1, similar to intertidal field conditions. At each position and at each shear stress applied, spores were counted through photo-analysis in ImageJ (version 1.48; U.S. National Institutes of Health, Bethesda, MD). With each successive application of shear stress, we expected an increasing percentage of spores to detach from the settlement plate, up to a maximum value assuming some spores would be stronger than our assay. The effect of shear stress on spore detachment in each species was tested using an exponential rise-to-maximum non-linear regression (SigmaPlot 11.0, R2 = 0.84 - 0.99), which provided estimates of two parameters: the maximum percentage of spores detached and the initial dependence of detachment on shear stress. The first derivatives of fitted, non-linear regressions were plotted to estimate frequency distributions of spore attachment strength.
attribute	NC_GLOBAL	awards_0_award_nid	String	55120
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1041213
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=1041213
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Mary Beth Saffo
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51608
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	The effect of pH on the attachment of Corallina vancouveriensis spores final PI: Emily Carrington Version: 2019-10-01
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-10-01T19:47:02Z
attribute	NC_GLOBAL	date_modified	String	2019-10-29T15:25:03Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.778426.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-123.14893
attribute	NC_GLOBAL	geospatial_lat_max	double	48.51359
attribute	NC_GLOBAL	geospatial_lat_min	double	48.51359
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-123.14893
attribute	NC_GLOBAL	geospatial_lon_min	double	-123.14893
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/778426
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	camera
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Prior to each test, the shear flume was fitted on the settlement plate with clamps and the released spores were photographed using a microscope (Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix S3300), using the lines drawn in the working section for reference.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	778437
attribute	NC_GLOBAL	instruments_0_description	String	All types of photographic equipment including stills, video, film and digital systems.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/311/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Camera
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	520
attribute	NC_GLOBAL	instruments_0_supplied_name	String	camera (Nikon Coolpix S3300)
attribute	NC_GLOBAL	instruments_1_acronym	String	Pressure Sensor
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Pressure gradient across the working section (measured with a manometer).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	778435
attribute	NC_GLOBAL	instruments_1_description	String	A pressure sensor is a device used to measure absolute, differential, or gauge pressures. It is used only when detailed instrument documentation is not available.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Pressure Sensor
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	567
attribute	NC_GLOBAL	instruments_1_supplied_name	String	manometer
attribute	NC_GLOBAL	instruments_2_acronym	String	Water Temp Sensor
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Specifically, spectrophotometric pH was determined as per SOP6 and total alkalinity was measured using an open cell titrator as outlined in SOP3b. As described in Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication 3, 191 pp.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	778451
attribute	NC_GLOBAL	instruments_2_description	String	General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/134/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Water Temperature Sensor
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	647
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Thermometer
attribute	NC_GLOBAL	instruments_3_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Specifically, spectrophotometric pH was determined as per SOP6. As described in Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication 3, 191 pp.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	778434
attribute	NC_GLOBAL	instruments_3_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_3_supplied_name	String	pH meter
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Prior to each test, the shear flume was fitted on the settlement plate with clamps and the released spores were photographed using a microscope (Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix S3300), using the lines drawn in the working section for reference.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	778436
attribute	NC_GLOBAL	instruments_4_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_4_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	708
attribute	NC_GLOBAL	instruments_4_supplied_name	String	microscope (Steindorff SXC, New York Microscopes)
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, latitude, longitude, management, oceanography, office, percent, PercentRemaining, preliminary, remaining, shear, species, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/778426/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/778426
attribute	NC_GLOBAL	Northernmost_Northing	double	48.51359
attribute	NC_GLOBAL	param_mapping	String	{'778426': {'lat': 'flag - latitude', 'lon': 'flag - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/778426/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Washington
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	FHL
attribute	NC_GLOBAL	people_0_person_name	String	Emily Carrington
attribute	NC_GLOBAL	people_0_person_nid	String	51609
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of British Columbia
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UBC-Bot
attribute	NC_GLOBAL	people_1_person_name	String	Patrick Martone
attribute	NC_GLOBAL	people_1_person_nid	String	710046
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of British Columbia
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UBC-Bot
attribute	NC_GLOBAL	people_2_person_name	String	Rebecca Guenther
attribute	NC_GLOBAL	people_2_person_nid	String	710051
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Mathew Biddle
attribute	NC_GLOBAL	people_3_person_nid	String	708682
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	OA - Ecomaterials Perspective
attribute	NC_GLOBAL	projects_0_acronym	String	OA - Ecomaterials Perspective
attribute	NC_GLOBAL	projects_0_description	String	Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective This award will support researchers based at the University of Washington's Friday Harbor Laboratories. The overall focus of the project is to determine how ocean acidification affects the integrity of biomaterials and how these effects in turn alter interactions among members of marine communities. The research plan emphasizes an ecomaterial approach; a team of biomaterials and ecomechanics experts will apply their unique perspective to detail how different combinations of environmental conditions affect the structural integrity and ecological performance of organisms. The study targets a diversity of ecologically important taxa, including bivalves, snails, crustaceans, and seaweeds, thereby providing insight into the range of possible biological responses to future changes in climate conditions. The proposal will enhance our understanding of the ecological consequences of climate change, a significant societal problem. Each of the study systems has broader impacts in fields beyond ecomechanics. Engineers are particularly interested in biomaterials and in each system there are materials with commercial potential. The project will integrate research and education by supporting doctoral student dissertation research, providing undergraduate research opportunities via three training programs at FHL, and summer internships for talented high school students, recruited from the FHL Science Outreach Program. The participation of underrepresented groups will be broadened by actively recruiting URM and female students. Results will be disseminated in a variety of forums, including peer-reviewed scientific publications, undergraduate and graduate course material, service learning activities in K-8 classrooms, demonstrations at FHL's annual Open House, and columns for a popular science magazine.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Friday Harbor, WA
attribute	NC_GLOBAL	projects_0_name	String	Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
attribute	NC_GLOBAL	projects_0_project_nid	String	2250
attribute	NC_GLOBAL	projects_0_project_website	String	http://depts.washington.edu/fhl/oael.html
attribute	NC_GLOBAL	projects_0_start_date	String	2010-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	48.51359
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	latitude,longitude,location
attribute	NC_GLOBAL	summary	String	Pterosiphonia bipinnata and Corallina vancouveriensis final experiment shear and percent remaining at Deadman Bay on San Juan Island, Washington
attribute	NC_GLOBAL	title	String	[Final spores] - Pterosiphonia bipinnata and Corallina vancouveriensis final experiment shear and percent remaining at Deadman Bay on San Juan Island, Washington (Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-123.14893
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Treatment		String
attribute	Treatment	bcodmo_name	String	treatment
attribute	Treatment	description	String	High (7.8) or Low (7.3) pH conditions
attribute	Treatment	long_name	String	Treatment
attribute	Treatment	units	String	unitless
variable	Shear		byte
attribute	Shear	_FillValue	byte	127
attribute	Shear	actual_range	byte	1, 20
attribute	Shear	bcodmo_name	String	unknown
attribute	Shear	description	String	Shear force applied
attribute	Shear	long_name	String	Shear
attribute	Shear	units	String	Pascals (Pa)
variable	PercentRemaining		double
attribute	PercentRemaining	_FillValue	double	NaN
attribute	PercentRemaining	actual_range	double	0.0, 1.0
attribute	PercentRemaining	bcodmo_name	String	unknown
attribute	PercentRemaining	colorBarMaximum	double	100.0
attribute	PercentRemaining	colorBarMinimum	double	0.0
attribute	PercentRemaining	description	String	Percentage of spores remaining after assay
attribute	PercentRemaining	long_name	String	Percent Remaining
attribute	PercentRemaining	units	String	unitless
variable	Species		String
attribute	Species	bcodmo_name	String	species
attribute	Species	description	String	Latin name of seaweed
attribute	Species	long_name	String	Species
attribute	Species	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	48.51359, 48.51359
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	latitude with positive values indicating North
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-123.14893, -123.14893
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	longitude with negative values indicating West
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	location		String
attribute	location	bcodmo_name	String	site
attribute	location	description	String	name of the location
attribute	location	long_name	String	Location
attribute	location	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.