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Title Sum-
Institution Dataset ID
   set  data   graph     files  public Daily growth rates of 8 populations of Chaetoceros simplex grown at 31C with control
population at 25C, in regular L1 medium (884 \u00b5m NO3-)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Chaetoceros simplex cultures, were obtained from population strain CCMP 200
(National Center for Marine Algae and Microbiota, NCMA).

Evolution experiment:
Eight populations were grown at 31 \u00b0C, and one was maintained as a
control at 25 \u00b0C in regular L1 medium (884 \u03bcm NO3\u2212).

At 31 \u00b0C, four populations remained in regular L1 medium (884 \u03bcm
NO3\u2212), while the other four received nitrogen\u2010reduced L1 medium (5
\u03bcm NO3\u2212);

Populations were maintained in 50 mL polycarbonate culture flasks, at 100
\u03bcmol quanta m\u22122 s\u22121 cool white fluorescent light on a 14/10 h
day/night cycle. We gently inverted and randomly repositioned flasks daily.
Every three days c. 10^6 cells (never < 6 \u00d7 10^5 cells) from each
population were transferred to fresh media. We monitored populations by
measuring in vivo optical density daily (436 nm wavelength absorbance) using a
Shimadzu UV\u20102401PC spectrophotometer before and after each transfer

Growth rate calculations:
When more than two biomass observations (optical density or fluorescence,
depending on the experiment) within the exponential growth phase were
available, we calculated population growth rates (day\u22121), as the slope of
the linear regression of ln(biomass) vs. time (days). Alternatively, when
biomass measurements were made every 2\u20133 days, we calculated growth rate


where B is biomass and t is time (days) and the number of generations within a
particular time range, \u0394t, as


where u is growth rate.\u00a0

Calculations were made with R, and scripts can be downloaded from:
More details in Aranguren-Gassis et al. 2019, Ecology Letters.
attribute NC_GLOBAL awards_0_award_nid String 712786
attribute NC_GLOBAL awards_0_award_number String OCE-1638958
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638958 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 712792
attribute NC_GLOBAL awards_1_award_number String OCE-1638804
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638804 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL awards_2_award_nid String 712795
attribute NC_GLOBAL awards_2_award_number String OCE-1638834
attribute NC_GLOBAL awards_2_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638834 (external link)
attribute NC_GLOBAL awards_2_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_2_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_2_funding_source_nid String 355
attribute NC_GLOBAL awards_2_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_2_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Growth Rates from Evolution Experiment
Daily growth rates of 8 populations of Chaetoceros simplex grown at 31C
with control population at 25C, in regular L1 medium (884 um NO3-)
P.I.'s: M. Aranguren-Gassis (U. Vigo), E. Litchman (MSU), C. Klausmeier (MSU)
version date: 2019-10-07
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-10-09T16:42:38Z
attribute NC_GLOBAL date_modified String 2019-10-30T17:29:31Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.778869.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/778869 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 778878
attribute NC_GLOBAL instruments_0_description String The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_instrument_nid String 595
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu UV‐2401PC spectrophotometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, concentration, data, dataset, dmo, erddap, evol, Evol_strain, growth, Growth_rate, management, n02, nitrate, Nitrate_Concentration, no3, oceanography, office, period, preliminary, rate, replicate, strain, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/778869/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/778869 (external link)
attribute NC_GLOBAL param_mapping String {'778869': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/778869/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Michigan State University
attribute NC_GLOBAL people_0_affiliation_acronym String MSU
attribute NC_GLOBAL people_0_person_name String Elena Litchman
attribute NC_GLOBAL people_0_person_nid String 543190
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Michigan State University
attribute NC_GLOBAL people_1_affiliation_acronym String MSU
attribute NC_GLOBAL people_1_person_name String Christopher Klausmeier
attribute NC_GLOBAL people_1_person_nid String 543192
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Michigan State University
attribute NC_GLOBAL people_2_affiliation_acronym String MSU
attribute NC_GLOBAL people_2_person_name String Colin T. Kremer
attribute NC_GLOBAL people_2_person_nid String 779889
attribute NC_GLOBAL people_2_role String Scientist
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Universidad de Vigo
attribute NC_GLOBAL people_3_person_name String Maria Aranguren-Gassis
attribute NC_GLOBAL people_3_person_nid String 778758
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Phytoplankton Community Responses
attribute NC_GLOBAL projects_0_acronym String Phytoplankton Community Responses
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:
Photosynthetic marine microbes, phytoplankton, contribute half of global primary production, form the base of most aquatic food webs and are major players in global biogeochemical cycles. Understanding their community composition is important because it affects higher trophic levels, the cycling of energy and elements and is sensitive to global environmental change. This project will investigate how phytoplankton communities respond to two major global change stressors in aquatic systems: warming and changes in nutrient availability. The researchers will work in two marine systems with a long history of environmental monitoring, the temperate Narragansett Bay estuary in Rhode Island and a subtropical North Atlantic site near Bermuda. They will use field sampling and laboratory experiments with multiple species and varieties of phytoplankton to assess the diversity in their responses to different temperatures under high and low nutrient concentrations. If the diversity of responses is high within species, then that species may have a better chance to adapt to rising temperatures and persist in the future. Some species may already be able to grow at high temperatures; consequently, they may become more abundant as the ocean warms. The researchers will incorporate this response information in mathematical models to predict how phytoplankton assemblages would reorganize under future climate scenarios. Graduate students and postdoctoral associates will be trained in diverse scientific approaches and techniques such as shipboard sampling, laboratory experiments, genomic analyses and mathematical modeling. The results of the project will be incorporated into K-12 teaching, including an advanced placement environmental science class for underrepresented minorities in Los Angeles, data exercises for rural schools in Michigan and disseminated to the public through an environmental journalism institute based in Rhode Island.
Predicting how ecological communities will respond to a changing environment requires knowledge of genetic, phylogenetic and functional diversity within and across species. This project will investigate how the interaction of phylogenetic, genetic and functional diversity in thermal traits within and across a broad range of species determines the responses of marine phytoplankton communities to rising temperature and changing nutrient regimes. High genetic and functional diversity within a species may allow evolutionary adaptation of that species to warming. If the phylogenetic and functional diversity is higher across species, species sorting and ecological community reorganization is likely. Different marine sites may have a different balance of genetic and functional diversity within and across species and, thus, different contribution of evolutionary and ecological responses to changing climate. The research will be conducted at two long-term time series sites in the Atlantic Ocean, the Narragansett Bay Long-Term Plankton Time Series and the Bermuda Atlantic Time Series (BATS) station. The goal is to assess intra- and inter-specific genetic and functional diversity in thermal responses at contrasting nutrient concentrations for a representative range of species in communities at the two sites in different seasons, and use this information to parameterize eco-evolutionary models embedded into biogeochemical ocean models to predict responses of phytoplankton communities to projected rising temperatures under realistic nutrient conditions. Model predictions will be informed by and tested with field data, including the long-term data series available for both sites and in community temperature manipulation experiments. This project will provide novel information on existing intraspecific genetic and functional thermal diversity for many ecologically and biogeochemically important phytoplankton species, estimate generation of new genetic and functional diversity in evolution experiments, and develop and parameterize novel eco-evolutionary models interfaced with ocean biogeochemical models to predict future phytoplankton community structure. The project will also characterize the interaction of two major global change stressors, warming and changing nutrient concentrations, as they affect phytoplankton diversity at functional, genetic, and phylogenetic levels. In addition, the project will develop novel modeling methodology that will be broadly applicable to understanding how other types of complex ecological communities may adapt to a rapidly warming world.
attribute NC_GLOBAL projects_0_end_date String 2020-09
attribute NC_GLOBAL projects_0_geolocation String Narragansett Bay, RI and Bermuda, Bermuda Atlantic Time-series Study (BATS)
attribute NC_GLOBAL projects_0_name String Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients
attribute NC_GLOBAL projects_0_project_nid String 712787
attribute NC_GLOBAL projects_0_start_date String 2016-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String Temperature
attribute NC_GLOBAL summary String Daily growth rates of 8 populations of Chaetoceros simplex grown at 31C and control population at 25C, in regular L1 medium (884 \u03bcm NO3\u2212) or nitrogen\u2010reduced L1 medium (5 \u03bcm NO3\u2212).
attribute NC_GLOBAL title String Daily growth rates of 8 populations of Chaetoceros simplex grown at 31C with control population at 25C, in regular L1 medium (884 \u00b5m NO3-)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Evol_strain   String  
attribute Evol_strain bcodmo_name String sample
attribute Evol_strain description String evolved population identifier; L1 signifies strains raised in 'regular' medium at 884 micromoles nitrate; 5 signifies medium with reduced nitrate at 5 micromoles; last number is replicate
attribute Evol_strain long_name String Evol Strain
attribute Evol_strain nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Evol_strain units String unitless
variable period   byte  
attribute period _FillValue byte 127
attribute period actual_range byte 1, 84
attribute period bcodmo_name String unknown
attribute period description String Culture transfer for which the rate is calculated
attribute period long_name String Period
attribute period units String unitless
variable Temperature   byte  
attribute Temperature _FillValue byte 127
attribute Temperature actual_range byte 31, 31
attribute Temperature bcodmo_name String temperature
attribute Temperature description String Culture maintenance temperature
attribute Temperature long_name String Temperature
attribute Temperature nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temperature units String Celsius degrees
variable Nitrate_Concentration   String  
attribute Nitrate_Concentration bcodmo_name String treatment
attribute Nitrate_Concentration description String Culture media nitrate concentration; L1 signifies 'regular' medium at 884 micromoles nitrate; 5 signifies reduced nitrate at 5 micromoles
attribute Nitrate_Concentration long_name String Nitrate Concentration
attribute Nitrate_Concentration units String Micromolar
variable Replicate   byte  
attribute Replicate _FillValue byte 127
attribute Replicate actual_range byte 1, 4
attribute Replicate bcodmo_name String replicate
attribute Replicate description String Replicate number
attribute Replicate long_name String Replicate
attribute Replicate units String unitless
variable Growth_rate   float  
attribute Growth_rate _FillValue float NaN
attribute Growth_rate actual_range float -0.2, 3.0
attribute Growth_rate bcodmo_name String growth
attribute Growth_rate description String Growth rate calculated from biomass
attribute Growth_rate long_name String Growth Rate
attribute Growth_rate units String day-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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