ERDDAP > info > bcodmo_dataset_779043

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	Prey-only exposure results from light stress in phytoplankton and dinoflagellate grazing response experiments from July of 2015 to July of 2017		I   M	background			BCO-DMO	bcodmo_dataset_779043

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Phytoplankton light stress \u2013 dinoflagellate grazing experiments General information Emiliania huxleyi strains were grown in f/50 without added Si, except for CCMP1516 which was grown in f/2 for experiments D and I and in f/50 otherwise. All other phytoplankton were grown in f/2 medium without added Si. Most strains (designated CCMP) were obtained from the National Center for Marine Algae and Microbiota except Heterocapsa rotundata, which was from the Norwegian Culture Collection of Algae (NORCCA). Heterotrophic dinoflagellates Amphidinium longum and Oxyrrhis marina were isolated from marine waters of the Salish Sea, grown in ciliate medium (Gifford 1985), and maintained on a mixture of phytoflagellate species. All cultures of any type were grown at a salinity of 30 and a temperature of 15\u00b0C. Phytoplankton were grown at a range of low to moderate irradiances, depending on experiment on a 12L:12D cycle. Heterotrophic dinoflagellates were grown at 10-20 \u00b5mol photons m-2 s-1 on a 12L:12D cycle. Before use in experiments, dinoflagellate predators were fed only Rhodomonas sp. 755 (A. longum) or Dunaliella tertiolecta (O. marina) and allowed to consume these prey until they were nearly gone from the culture. Cells were exposed to experimental light treatments outdoors in a shallow tank filled with flowing seawater supplied from nearby coastal waters. Temperature during experiments was monitored at regular intervals with a thermometer mounted in an unscreened incubation bottle, and ranged from 14-15\u00b0C except for Exp. A, where it averaged 17\u00b0C. Light (incident photosynthetically active radiation, or PAR) was measured with a Li-Cor 2\u03c0 sensor, and logged at 5-min intervals so that total experiment light dose (mol photons m-2) could be computed for specific incubation periods. Control treatments were incubated in 60-ml polycarbonate bottles screened with sufficient neutral density screening to approximate growth irradiances. Higher light exposures were achieved using fewer (or no) layers of neutral density screening, depending on experiment. Except for Exp. E, which used polycarbonate bottles only, all high light treatments used 60-ml Teflon bottles, which are transparent to UV wavelengths. In some experiments high light treatments included both Teflon (UV-transparent) and polycarbonate (UV- opaque) bottles, to isolate the effects of UV on protist responses. Bottles were incubated at ~10 cm depth in the outdoor tank. Experiments A-F exposed only the phytoplankton prey to the light stress treatments (\u2018Single_factor_grazing (prey-only)\u2019 data set [https://www.bco-dmo.org/dataset/779043](\\"https://www.bco- dmo.org/dataset/779043\\")). Cultures were divided into incubation bottles (n=3-5 depending on experiment) and placed in the outdoor tank for 60-120 min. Photosynthetic efficiency (Fv/Fm) was monitored before cells were taken outside (t=0) and, after gentle mixing, at 30-min intervals during the incubations (\u2018FvFm\u2019 data set [https://www.bco- dmo.org/dataset/779033](\\"https://www.bco-dmo.org/dataset/779033\\")). After outdoor exposure, phytoplankton were returned to the laboratory and a subsample from each replicate was added to a corresponding 30-ml polycarbonate bottle containing heterotrophic dinoflagellate predator A. longum to initiate predation experiments. The remainder of the phytoplankton culture volume was placed in an incubator at the culture growth irradiance level, and Fv/Fm monitored at regular intervals during this recovery period. Prey concentrations for predation experiments ranged from 5.0 x 103 cells ml-1 for dinoflagellate Heterocapsa rotundata to 5.0 x 104 cells ml-1 for the various E. huxleyi strains. Prey biomass densities were equivalent for all prey types, at ~500 \u00b5g C liter-1. Carbon per cell for each phytoplankton species was estimated from measured cell volumes and published C:volume conversion factors (Menden-Deuer & Lessard 2000). A. longum concentrations were ~1-2 x 103 cells ml-1, and O. marina concentration (Exp. I only, see below) was 260 cells ml-1. For \u2018prey only exposure\u2019 experiments, predation tests were conducted for 50 min in a laboratory incubator at 15\u00b0C and ~50 \u00b5mol photons m-2 s-1. For \u2018prey and predator exposure\u2019 experiments, predation tests were conducted for 40-60 min under either control or high light outdoor illumination conditions. Predation tests were terminated by adding cells to cold 10% glutaraldehyde and DAPI stain (final concentrations 0.5% and 0.1 \u00b5g ml-1, respectively). After fixation overnight in 4\u00b0C and darkness, samples were filtered (3 or 5 \u00b5m pore-size polycarbonate filters), mounted on slides, and frozen for later examination by epifluorescence microscopy. UV excitation was used to locate and identify dinoflagellate predators from the DAPI-induced fluorescence of their nuclei. Ingested prey were detected using blue light excitation, from the orange (cryptophyte) or red (all other prey) autofluorescence of the prey pigments inside the predator food vacuoles Because A. longum uses a peduncle to feed on cryptophytes, rather than phagocytizing intact cells, the number of ingested prey per predator cannot be quantified for this predator \u2013 prey combination. Therefore for all predator and prey types, each micrograzer cell was scored as \u2018feeding\u2019 or \u2018not feeding\u2019. At least 250 micrograzers per slide were scored; predation intensity was calculated as fraction of the population feeding (= # micrograzers with ingested prey / total # micrograzers scored). Experiments G, H, and I used a matrix design in which predators and prey were exposed to experimental irradiances separately, then combined in various ways and predation measured in outdoor irradiance conditions (\u2018Prey and predator exposure\u2019 data set [https://www.bco- dmo.org/dataset/779050](\\"https://www.bco-dmo.org/dataset/779050\\")). Cultures of predators and prey were incubated in separate bottles for the first 1-1.2 h of exposure time. After that, appropriate volumes of prey with various exposure histories were introduced into predator bottles with various exposure histories, and those predation tests incubated for an additional 40-60 min at the original predator irradiance level. Fv/Fm was monitored throughout (\u2018Photosynthetic efficiency\u2019 data set [https://www.bco- dmo.org/dataset/779033](\\"https://www.bco-dmo.org/dataset/779033\\")), first in the original phytoplankton-only bottles and then in the remaining phytoplankton volume after predation tests were initiated, and finally through a recovery period in the laboratory as described above. At the end of the predation test period, samples were fixed and slides prepared as described above. For more information see Strom et al. (2020).
attribute	NC_GLOBAL	awards_0_award_nid	String	614837
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1434842
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1434842
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Prey-only exposure results from light stress PI: Suzanne Strom Data Version 1: 2019-10-15
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-10-11T16:12:49Z
attribute	NC_GLOBAL	date_modified	String	2020-03-19T20:25:53Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.779043.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/779043
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	LI-COR Biospherical PAR
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Irradiance measurements: Li-Cor 1400 data logger with 2-pi (cosine) photosynthetically active radiation (PAR) sensor
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	779049
attribute	NC_GLOBAL	instruments_0_description	String	The LI-COR Biospherical PAR Sensor is used to measure Photosynthetically Available Radiation (PAR) in the water column. This instrument designation is used when specific make and model are not known.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0074/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	LI-COR Biospherical PAR Sensor
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	480
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Li-Cor 1400 data logger with 2-pi (cosine) photosynthetically active radiation (PAR) sensor
attribute	NC_GLOBAL	instruments_1_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Photosynthetic efficiency measurements: Pulse-Amplitude Modulated Fluorometer: Walz Water PAM
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	779048
attribute	NC_GLOBAL	instruments_1_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Pulse-Amplitude Modulated Fluorometer: Walz Water PAM
attribute	NC_GLOBAL	keywords	String	available, bco, bco-dmo, biological, bottle, Bottle_Type, chemical, data, dataset, date, dmo, dose, erddap, experiment, Experiment_ID, feeding, fraction, Fraction_Feeding, management, micrograzer, Micrograzer_Species, num, Num_Screens, number, oceanography, office, par, photosynthetically, phytoplankton, Phytoplankton_PAR_Dose, Phytoplankton_Species, preliminary, radiation, replicate, Replicate_Number, screens, species, time, type
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/779043/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/779043
attribute	NC_GLOBAL	param_mapping	String	{'779043': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/779043/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Washington
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UW
attribute	NC_GLOBAL	people_0_person_name	String	Suzanne Strom
attribute	NC_GLOBAL	people_0_person_nid	String	50471
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Amber York
attribute	NC_GLOBAL	people_1_person_nid	String	643627
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Protist signaling
attribute	NC_GLOBAL	projects_0_acronym	String	Protist signaling
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF proposal: This proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of Phaeodactylum tricornutum light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species. Our work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Salish Sea: 48.5, -122.75
attribute	NC_GLOBAL	projects_0_name	String	Environmental stress and signaling based on reactive oxygen species among planktonic protists
attribute	NC_GLOBAL	projects_0_project_nid	String	614838
attribute	NC_GLOBAL	projects_0_start_date	String	2014-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	Micrograzer_Species
attribute	NC_GLOBAL	summary	String	Prey-only exposure results (single-factor) results from light stress in phytoplankton and dinoflagellate grazing response experiments from July of 2015 to July of 2017. These data were published in Strom et al. (2020).
attribute	NC_GLOBAL	title	String	Prey-only exposure results from light stress in phytoplankton and dinoflagellate grazing response experiments from July of 2015 to July of 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Experiment_ID		String
attribute	Experiment_ID	bcodmo_name	String	exp_id
attribute	Experiment_ID	description	String	Shows letter (A-I) corresponding to experiment ID system used in Strom et al. (submitted), followed by experiment ID used in Strom lab.
attribute	Experiment_ID	long_name	String	Experiment ID
attribute	Experiment_ID	units	String	unitless
variable	Experiment_Date		String
attribute	Experiment_Date	bcodmo_name	String	date
attribute	Experiment_Date	description	String	Calendar date on which experiment was conducted ISO 8601 Date format yyyy-mm-dd
attribute	Experiment_Date	long_name	String	Experiment Date
attribute	Experiment_Date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Experiment_Date	source_name	String	Experiment_Date
attribute	Experiment_Date	time_precision	String	1970-01-01
attribute	Experiment_Date	units	String	unitless
variable	Phytoplankton_Species		String
attribute	Phytoplankton_Species	bcodmo_name	String	animal_group
attribute	Phytoplankton_Species	description	String	Shows species and strain number (CCMP), where available, for phytoplankton used in light stress experiments. See species list (supplemental document) for species codes and the corresponding species names.
attribute	Phytoplankton_Species	long_name	String	Phytoplankton Species
attribute	Phytoplankton_Species	units	String	unitless
variable	Micrograzer_Species		String
attribute	Micrograzer_Species	bcodmo_name	String	animal_group
attribute	Micrograzer_Species	description	String	Shows species and strain number (CCMP), where available, for micrograzers used in light stress experiments. See species list (supplemental document) for species codes and the corresponding species names.
attribute	Micrograzer_Species	long_name	String	Micrograzer Species
attribute	Micrograzer_Species	units	String	unitless
variable	Bottle_Type		String
attribute	Bottle_Type	bcodmo_name	String	bottle
attribute	Bottle_Type	description	String	Composition of bottles used for outdoor light exposure; PC = polycarbonate
attribute	Bottle_Type	long_name	String	Bottle Type
attribute	Bottle_Type	units	String	unitless
variable	Num_Screens		byte
attribute	Num_Screens	_FillValue	byte	127
attribute	Num_Screens	actual_range	byte	0, 6
attribute	Num_Screens	bcodmo_name	String	sample_descrip
attribute	Num_Screens	description	String	Number of neutral density screen layers used to wrap bottles during outdoor exposure period
attribute	Num_Screens	long_name	String	Num Screens
attribute	Num_Screens	units	String	unitless
variable	Phytoplankton_PAR_Dose		float
attribute	Phytoplankton_PAR_Dose	_FillValue	float	NaN
attribute	Phytoplankton_PAR_Dose	actual_range	float	0.1, 9.11
attribute	Phytoplankton_PAR_Dose	bcodmo_name	String	PAR
attribute	Phytoplankton_PAR_Dose	description	String	Total dose of photosynthetically active radiation received by phytoplankton during outdoor ‘light stress’ incubation period, before phytoplankton prey were added to micrograzers under low light laboratory conditions.
attribute	Phytoplankton_PAR_Dose	long_name	String	Phytoplankton PAR Dose
attribute	Phytoplankton_PAR_Dose	units	String	mol photons m-2
variable	Replicate_Number		byte
attribute	Replicate_Number	_FillValue	byte	127
attribute	Replicate_Number	actual_range	byte	1, 4
attribute	Replicate_Number	bcodmo_name	String	replicate
attribute	Replicate_Number	colorBarMaximum	double	100.0
attribute	Replicate_Number	colorBarMinimum	double	0.0
attribute	Replicate_Number	description	String	Identifies an individual replicate bottle
attribute	Replicate_Number	long_name	String	Replicate Number
attribute	Replicate_Number	units	String	unitless
variable	Fraction_Feeding		float
attribute	Fraction_Feeding	_FillValue	float	NaN
attribute	Fraction_Feeding	actual_range	float	0.05, 0.884
attribute	Fraction_Feeding	bcodmo_name	String	unknown
attribute	Fraction_Feeding	colorBarMaximum	double	1.0
attribute	Fraction_Feeding	colorBarMinimum	double	0.0
attribute	Fraction_Feeding	description	String	Fraction of the total enumerated micrograzer population that contained ingested phytoplankton prey after the 50-min predation test period in the laboratory.
attribute	Fraction_Feeding	long_name	String	Fraction Feeding
attribute	Fraction_Feeding	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.