ERDDAP > info > bcodmo_dataset_779425

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms		I   M	background			BCO-DMO	bcodmo_dataset_779425

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Detailed methods can be found in McCormick, LR; Levin, LA; Oesch, NW. 2019. Vision is highly sensitive to oxygen availability in marine invertebrate larvae. Journal of Experimental Biology 222, jeb200899. doi:10.1242/jeb.200899. Data shown in Figure 1, bottom panel. Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 \u03bcmol photons m\u22122 s\u22121 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in \u201cnormoxia\u201d (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows the three visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function. V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels). Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.
attribute	NC_GLOBAL	awards_0_award_nid	String	775842
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1829623
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1829623
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Oxygen metrics PI: Lisa A. Levin Data Version 1: 2019-10-29
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-10-23T19:47:50Z
attribute	NC_GLOBAL	date_modified	String	2019-11-01T15:44:49Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.779425.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/779425
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	O2 microsensor
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	779903
attribute	NC_GLOBAL	instruments_0_description	String	A miniaturized Clark-type dissolved oxygen instrument, including glass micro-sensors with minute tips (diameters ranging from 1 to 800 um). A gold or platinum sensing cathode is polarized against an internal reference and, driven by external partial pressure, oxygen from the environment penetrates through the sensor tip membrane and is reduced at the sensing cathode surface. A picoammeter converts the resulting reduction current to a signal. The size of the signal generated by the electrode is proportional to the flux of oxygen molecules to the cathode.The sensor also includes a polarized guard cathode, which scavenges oxygen in the electrolyte, thus minimizing zero-current and pre-polarization time.With the addition of a meter and a sample chamber, the respiration of a small specimen can be measured. Example: Strathkelvin Inst. http://www.strathkelvin.com
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/351/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Oxygen Microelectrode Sensor
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	701
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, experiment, Experiment_Name, management, metric, name, O2, O2_ml_l, O2_pO2, O2_umol_kg, O2_umol_l, oceanography, office, oxygen, preliminary, species, umol
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/779425/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/779425
attribute	NC_GLOBAL	param_mapping	String	{'779425': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/779425/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-San Diego
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UCSD
attribute	NC_GLOBAL	people_0_person_name	String	Lisa A Levin
attribute	NC_GLOBAL	people_0_person_nid	String	51242
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of California-San Diego
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UCSD
attribute	NC_GLOBAL	people_1_person_name	String	Nicholas Oesch
attribute	NC_GLOBAL	people_1_person_nid	String	775846
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Karen Soenen
attribute	NC_GLOBAL	people_2_person_nid	String	748773
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	Vision under hypoxia
attribute	NC_GLOBAL	projects_0_acronym	String	Vision under hypoxia
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: Oxygen is being lost in the ocean worldwide as a result of ocean warming and the input of nutrients from land. Vision requires a large amount of oxygen, and may be less effective or require more light when oxygen is in short supply. This is especially true for active marine animals with complex eyes and visual capabilities, including active arthropods (crabs), cephalopods (squid), and fish. The California coastal waters exhibit a sharp drop in oxygen and light with increasing water depth. This project examines how visual physiology and ecology in young (larval) highly visual marine animals respond to oxygen loss, with a focus on key fisheries and aquaculture species. Experiments and observations will test the hypothesis that oxygen stress will change the light required for these organisms to see effectively, influencing the water depths where they can live and survive. The project will provide interdisciplinary experiences to students and an early career scientist and inform both the public (through outreach at the Birch Aquarium at Scripps Institution of Oceanography) and policy makers about the effects of oxygen decline in the ocean. Negative effects of oxygen loss on vision have been described for humans and other terrestrial organisms, but never in the marine environment, despite the large changes in oxygen that can occur with depth and over time in the ocean, and the high metabolic demand of visual systems. This project will test the effects of low oxygen on vision in 3 combinations of eye design and photo-transduction mechanisms: compound eye with rhabdomeric photoreceptors (arthropods), simple eye with rhabdomeric photoreceptors (cephalopods), and simple eye with ciliary photoreceptors (fish). A series of oxygen- and light-controlled laboratory experiments will be conducted on representative taxa of each group including the tuna crab, Pleuroncodes planipes; the market squid, Doryteuthis opalescens, and the white sea bass, Atractoscion nobilis. In vivo electrophysiology and behavioral phototaxis experiments will identify new oxygen metrics for visual physiology and function, and will be compared to metabolic thresholds determined in respiration experiments. Hydrographic data collected over 3 decades by the CalCOFI program in the Southern California Bight will be evaluated with respect to visual and metabolic limits to determine the consequences of oxygen variation on the critical luminoxyscape (range of oxygen and light conditions required for visual physiology and function in target species) boundary in each species. Findings for the three vision-based functional groups may test whether oxygen-limited visual responses offer an additional explanation for the shoaling of species distributions among highly visual pelagic taxa in low oxygen, and will help to focus future research efforts and better understand the stressors contributing to habitat compression with expanding oxygen loss in the ocean. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-09
attribute	NC_GLOBAL	projects_0_geolocation	String	Southern California Bight, Northeast Pacific Ocean
attribute	NC_GLOBAL	projects_0_name	String	Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
attribute	NC_GLOBAL	projects_0_project_nid	String	775843
attribute	NC_GLOBAL	projects_0_start_date	String	2018-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms
attribute	NC_GLOBAL	title	String	Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Species		String
attribute	Species	bcodmo_name	String	species
attribute	Species	description	String	Species name.
attribute	Species	long_name	String	Species
attribute	Species	units	String	unitless
variable	Experiment_Name		String
attribute	Experiment_Name	bcodmo_name	String	exp_id
attribute	Experiment_Name	description	String	Original experiment name that can be linked back to raw datafile collected in Igor.
attribute	Experiment_Name	long_name	String	Experiment Name
attribute	Experiment_Name	units	String	unitless
variable	Metric		String
attribute	Metric	bcodmo_name	String	unknown
attribute	Metric	description	String	One of three oxygen metrics for retinal function calculated in McCormick et al.
attribute	Metric	long_name	String	Metric
attribute	Metric	units	String	unitless
variable	O2_umol_l		float
attribute	O2_umol_l	_FillValue	float	NaN
attribute	O2_umol_l	actual_range	float	52.858, 278.834
attribute	O2_umol_l	bcodmo_name	String	dissolved Oxygen
attribute	O2_umol_l	description	String	Oxygen concentration in umol O2/L of the given metric.
attribute	O2_umol_l	long_name	String	O2 Umol L
attribute	O2_umol_l	units	String	micromole per liter (umol/L)
variable	O2_ml_l		double
attribute	O2_ml_l	_FillValue	double	NaN
attribute	O2_ml_l	actual_range	double	1.258705603, 6.671416134
attribute	O2_ml_l	bcodmo_name	String	dissolved Oxygen
attribute	O2_ml_l	description	String	Oxygen concentration in mL O2/L of the given metric.
attribute	O2_ml_l	long_name	String	O2 Ml L
attribute	O2_ml_l	units	String	milliliter per liter (ml/l)
variable	O2_umol_kg		double
attribute	O2_umol_kg	_FillValue	double	NaN
attribute	O2_umol_kg	actual_range	double	51.55221122, 272.1230455
attribute	O2_umol_kg	bcodmo_name	String	dissolved Oxygen
attribute	O2_umol_kg	description	String	Oxygen concentration in umol O2/kg of the given metric.
attribute	O2_umol_kg	long_name	String	O2 Umol Kg
attribute	O2_umol_kg	units	String	micromole per kg (umol/kg)
variable	O2_pO2		double
attribute	O2_pO2	_FillValue	double	NaN
attribute	O2_pO2	actual_range	double	4.394383386, 23.32529845
attribute	O2_pO2	bcodmo_name	String	dissolved Oxygen
attribute	O2_pO2	description	String	The partial pressure of oxygen of the given metric.
attribute	O2_pO2	long_name	String	O2 P O2
attribute	O2_pO2	units	String	kiloPascale (kPa)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.