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Title Sum-
Institution Dataset ID
     data   graph     files  public Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-
Atlantic Ridge, from 2012-2014
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Sample collection
Crustal fluids were collected from the single horizon at U1382A and from the
shallow, middle and deep horizons in U1383C (Edwards et al., 2012) using a
mobile pumping system designed for microbial sampling from CORK fluid delivery
lines as described in Meyer et al. (2016) and Cowen et al. (2012). Deployed
with the ROV system, mobile pumping system connectors are attached to the CORK
wellhead via an umbilical to the hydrological zone of interest within the
aquifer. Fluid systems were flushed and allowed to equilibrate before
sampling, and dissolved oxygen concentrations were measured during pumping
using an Aanderaa sensor (Meyer et al., 2016). In 2012, 12 l of each fluid
sample were filtered on to a 0.22 \u03bcm Sterivex-GP filter (Merck Millipore,
Billerica, MA, USA) as described in Meyer et al. (2016). In 2014, 12 l of each
sample was filtered in situ and immediately fixed with RNALater (Thermo Fisher
Scientific, Waltham, MA, USA), as described previously (Akerman et al., 2013).
After sampling in 2012, a battery-powered GeoMICROBE sled was left at each
CORK for time series autonomous sampling of the fluid delivery lines (Cowen et
al., 2012). For each filter sample, ~10 l of fluid were filtered in situ and
immediately fixed with RNALater. For downstream analysis, ~500 ml of fluid
were filtered into two Tedlar bags, one containing 54 ml of 37% formaldehyde
for cell enumeration and the other with 4 ml of 10% HCl for inorganic
chemistry analyses. Sleds were deployed in April 2012 and recovered in April
2014 with samples collected. Upon sled recovery, filters were transferred to
fresh RNALater and stored at \u221280 \u00b0C, while all bag samples were
stored at 4 \u00b0C (Cowen et al., 2012). Deep bottom water was sampled in
2012 and 2014 via a CTD at 100 m above the seafloor and filtered in the same
manner as the crustal fluids onto Sterivex filters. Total microbial biomass in
fluids was enumerated with DAPI (4\u2032,6\u2032-diamidino-2-phenylindole;
Sigma-Aldrich, St Louis, MO, USA) and epifluorescent microscopy (Porter and
Feig, 1980). Fluids also were analyzed for dissolved silicon and nitrate using
automated colorimetric analysis and pH was measured with an electrode before a
potentiometric titration for the determination of alkalinity (Wheat et al.,
attribute NC_GLOBAL awards_0_award_nid String 554913
attribute NC_GLOBAL awards_0_award_number String OCE-1061934
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1061934 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL awards_1_award_nid String 554917
attribute NC_GLOBAL awards_1_award_number String OCE-1061827
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1061827 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String David L. Garrison
attribute NC_GLOBAL awards_1_program_manager_nid String 50534
attribute NC_GLOBAL awards_2_award_nid String 554921
attribute NC_GLOBAL awards_2_award_number String OCE-1062006
attribute NC_GLOBAL awards_2_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1062006 (external link)
attribute NC_GLOBAL awards_2_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_2_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_2_funding_source_nid String 355
attribute NC_GLOBAL awards_2_program_manager String David L. Garrison
attribute NC_GLOBAL awards_2_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Chemistry and cell counts of formation fluids from North Pond
PI: Julie Huber
Version: 2019-11-19
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-11-19T17:33:59Z
attribute NC_GLOBAL date_modified String 2019-12-02T21:07:15Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.782025.1
attribute NC_GLOBAL Easternmost_Easting double -46.05
attribute NC_GLOBAL geospatial_lat_max double 22.8
attribute NC_GLOBAL geospatial_lat_min double 22.78
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -46.05
attribute NC_GLOBAL geospatial_lon_min double -46.09
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/782025 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String CTD
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Deep bottom water was sampled in 2012 and 2014 via a CTD at 100 m above the seafloor and filtered in the same manner as the crustal fluids onto Sterivex filters.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 782032
attribute NC_GLOBAL instruments_0_description String The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/130/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String CTD profiler
attribute NC_GLOBAL instruments_0_instrument_nid String 417
attribute NC_GLOBAL instruments_0_supplied_name String CTD
attribute NC_GLOBAL instruments_1_acronym String AOO
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Fluid systems were flushed and allowed to equilibrate before sampling, and dissolved oxygen concentrations were measured during pumping using an Aanderaa sensor (Meyer et al., 2016).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 782033
attribute NC_GLOBAL instruments_1_description String Aanderaa Oxygen Optodes are instrument for monitoring oxygen in the environment. For instrument information see the Aanderaa Oxygen Optodes Product Brochure.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/351/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Aanderaa Oxygen Optodes
attribute NC_GLOBAL instruments_1_instrument_nid String 563
attribute NC_GLOBAL instruments_1_supplied_name String Aanderaa sensor
attribute NC_GLOBAL instruments_2_acronym String Automatic titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Fluids also were analyzed for dissolved silicon and nitrate using automated colorimetric analysis and pH was measured with an electrode before a potentiometric titration for the determination of alkalinity (Wheat et al., 2017).
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 782036
attribute NC_GLOBAL instruments_2_description String Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB12/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Automatic titrator
attribute NC_GLOBAL instruments_2_instrument_nid String 682
attribute NC_GLOBAL instruments_2_supplied_name String automated colorimetric analysis
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Total microbial biomass in fluids was enumerated with DAPI (4′,6′-diamidino-2-phenylindole; Sigma-Aldrich, St Louis, MO, USA) and epifluorescent microscopy (Porter and Feig, 1980).
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 782035
attribute NC_GLOBAL instruments_3_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_3_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_3_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_3_instrument_nid String 695
attribute NC_GLOBAL instruments_3_supplied_name String epifluorescent microscopy
attribute NC_GLOBAL instruments_4_acronym String GeoMICROBE
attribute NC_GLOBAL instruments_4_dataset_instrument_description String After sampling in 2012, a battery-powered GeoMICROBE sled was left at each CORK for time series autonomous sampling of the fluid delivery lines (Cowen et al., 2012).
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 782034
attribute NC_GLOBAL instruments_4_description String Integrated Ocean Drilling Program borehole CORK (Circulation Obviation Retrofit Kit) observatories provide long-term access to hydrothermal fluids circulating within the basaltic crust (basement), providing invaluable opportunities to study the deep biosphere. We describe the design and application parameters of the GeoMICROBE instrumented sled, an autonomous sensor and fluid sampling system. The GeoMICROBE system couples with CORK fluid delivery lines to draw large volumes of fluids from crustal aquifers to the seafloor. These fluids pass a series of in-line sensors and an in situ filtration and collection system. GeoMICROBE’s major components include a primary valve manifold system, a positive displacement primary pump, sensors (e.g., fluid flow rate, temperature, dissolved O2, electrochemistry-voltammetry analyzer), a 48-port in situ filtration and fluid collection system, computerized controller, seven 24 V-40 A batteries and wet-mateable (ODI) communications with submersibles. This constantly evolving system has been successfully connected to IODP Hole 1301A on the eastern flank of the Juan de Fuca Ridge. 

Reference: Cowen, J.P., Copson, D., Jolly, J., Hsieh, C.-C., Matsumoto, R., Glazer, B.T. et al. (2012) Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere., Deep-Sea Research I, 61: 43-56 doi:10.1016/j.dsr.2011.11.004
attribute NC_GLOBAL instruments_4_instrument_name String GeoMICROBE
attribute NC_GLOBAL instruments_4_instrument_nid String 565376
attribute NC_GLOBAL instruments_4_supplied_name String GeoMICROBE sled
attribute NC_GLOBAL keywords String 95pcnt, bco, bco-dmo, biological, bioproject, bioproject_id, cell, Cell_counts, chemical, chemistry, collection, concentration, confidence, confidence_level_95pcnt, counts, data, dataset, date, depth, depth_maximum, depth_minimum, description, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Chemistry > Silicate, erddap, latitude, level, longitude, management, mass, mass_concentration_of_silicate_in_sea_water, mole, mole_concentration_of_nitrate_in_sea_water, n02, nitrate, no3, O2, ocean, oceanography, oceans, office, oxygen, preliminary, sample, science, sea, seawater, silicate, time, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/782025/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/782025 (external link)
attribute NC_GLOBAL Northernmost_Northing double 22.8
attribute NC_GLOBAL param_mapping String {'782025': {'lat': 'flag - latitude', 'lon': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/782025/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Marine Biological Laboratory
attribute NC_GLOBAL people_0_affiliation_acronym String MBL
attribute NC_GLOBAL people_0_person_name String Julie Huber
attribute NC_GLOBAL people_0_person_nid String 51266
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Harvard University
attribute NC_GLOBAL people_1_person_name String Peter Girguis
attribute NC_GLOBAL people_1_person_nid String 544586
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Hawaii at Manoa
attribute NC_GLOBAL people_2_affiliation_acronym String SOEST
attribute NC_GLOBAL people_2_person_name String Brian Glazer
attribute NC_GLOBAL people_2_person_nid String 554919
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL project String North Pond Microbes
attribute NC_GLOBAL projects_0_acronym String North Pond Microbes
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Current estimates suggest that the volume of ocean crust capable of sustaining life is comparable in magnitude to that of the oceans. To date, there is little understanding of the composition or functional capacity of microbial communities in the sub-seafloor, or their influence on the chemistry of the oceans and subsequent consequences for global biogeochemical cycles. This project focuses on understanding the relationship between microbial communities and fluid chemistry in young crustal fluids that are responsible for the transport of energy, nutrients, and organisms in the crust. Specifically, the PIs will couple microbial activity measurements, including autotrophic carbon, nitrogen and sulfur metabolisms as well as mineral oxide reduction, with quantitative assessments of functional gene expression and geochemical transformations in basement fluids. Through a comprehensive suite of in situ and shipboard analyses, this research will yield cross-disciplinary advances in our understanding of the microbial ecology and geochemistry of the sub-seafloor biosphere. The focus of the effort is at North Pond, an isolated sediment pond located on ridge flank oceanic crust 7-8 million years old on the western side of the Mid-Atlantic Ridge. North Pond is currently the target for drilling on IODP expedition 336, during which it will be instrumented with three sub-seafloor basement observatories.
The project will leverage this opportunity for targeted and distinct sampling at North Pond on two German-US research cruises to accomplish three main objectives:
1. to determine if different basement fluid horizons across North Pond host distinct microbial communities and chemical milieus and the degree to which they change over a two-year post-drilling period.
2. to quantify the extent of autotrophic metabolism via microbially-mediated transformations in carbon, nitrogen, and sulfur species in basement fluids at North Pond.
3. to determine the extent of suspended particulate mineral oxides in basement fluids at North Pond and to characterize their role as oxidants for fluid-hosted microbial communities.
Specific outcomes include quantitative assessments of microbial activity and gene expression as well as geochemical transformations. The program builds on the integrative research goals for North Pond and will provide important data for guiding the development of that and future deep biosphere research programs. Results will increase understanding of microbial life and chemistry in young oceanic crust as well as provide new insights into controls on the distribution and activity of marine microbial communities throughout the worlds oceans.
There are no data about microbial communities in ubiquitous cold, oceanic crust, the emphasis of the proposed work. This is an interdisciplinary project at the interface of microbial ecology, chemistry, and deep-sea oceanography with direct links to international and national research and educational organizations.
attribute NC_GLOBAL projects_0_end_date String 2015-05
attribute NC_GLOBAL projects_0_geolocation String North Pond, mid-Atlantic Ridge
attribute NC_GLOBAL projects_0_name String Collaborative Research: Characterization of Microbial Transformations in Basement Fluids, from Genes to Geochemical Cycling
attribute NC_GLOBAL projects_0_project_nid String 554914
attribute NC_GLOBAL projects_0_start_date String 2011-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 22.78
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Chemistry and cell counts of formation fluids from North Pond
attribute NC_GLOBAL title String Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -46.09
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable sample   String  
attribute sample bcodmo_name String sample
attribute sample description String sample identifier
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable bioproject_id   String  
attribute bioproject_id bcodmo_name String accession number
attribute bioproject_id description String NIH bioproject term
attribute bioproject_id long_name String Bioproject Id
attribute bioproject_id units String unitless
variable collection_date   String  
attribute collection_date bcodmo_name String date
attribute collection_date description String date of fluid collection in yyyy-mm-dd format
attribute collection_date long_name String Collection Date
attribute collection_date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute collection_date source_name String collection_date
attribute collection_date time_precision String 1970-01-01
attribute collection_date units String unitless
variable description   String  
attribute description bcodmo_name String sample_descrip
attribute description description String Fluid type and origin
attribute description long_name String Description
attribute description units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 22.78, 22.8
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude of sample
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -46.09, -46.05
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude of sample
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth_minimum   short  
attribute depth_minimum _FillValue short 32767
attribute depth_minimum actual_range short 70, 4350
attribute depth_minimum bcodmo_name String depth_bsf
attribute depth_minimum colorBarMaximum double 8000.0
attribute depth_minimum colorBarMinimum double -8000.0
attribute depth_minimum colorBarPalette String TopographyDepth
attribute depth_minimum description String minimum range of sample depth within borehole below sea floor
attribute depth_minimum long_name String Depth
attribute depth_minimum standard_name String depth
attribute depth_minimum units String meters
variable depth_maximum   short  
attribute depth_maximum _FillValue short 32767
attribute depth_maximum actual_range short 146, 4350
attribute depth_maximum bcodmo_name String depth_bsf
attribute depth_maximum colorBarMaximum double 8000.0
attribute depth_maximum colorBarMinimum double -8000.0
attribute depth_maximum colorBarPalette String TopographyDepth
attribute depth_maximum description String maximum range of sample depth within borehole below sea floor
attribute depth_maximum long_name String Depth
attribute depth_maximum standard_name String depth
attribute depth_maximum units String meters
variable Cell_counts   short  
attribute Cell_counts _FillValue short 32767
attribute Cell_counts actual_range short 5000, 28000
attribute Cell_counts bcodmo_name String abundance
attribute Cell_counts description String cell counts
attribute Cell_counts long_name String Cell Counts
attribute Cell_counts nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute Cell_counts units String cells per mililiter (cells/mL)
variable confidence_level_95pcnt   short  
attribute confidence_level_95pcnt _FillValue short 32767
attribute confidence_level_95pcnt actual_range short 300, 11000
attribute confidence_level_95pcnt bcodmo_name String unknown
attribute confidence_level_95pcnt colorBarMaximum double 50.0
attribute confidence_level_95pcnt colorBarMinimum double 0.0
attribute confidence_level_95pcnt description String cell count confidence interval
attribute confidence_level_95pcnt long_name String Confidence Level 95pcnt
attribute confidence_level_95pcnt units String cells per mililiter (cells/mL)
variable O2   String  
attribute O2 bcodmo_name String dissolved Oxygen
attribute O2 description String Dissolved oxygen concentration
attribute O2 long_name String O2
attribute O2 units String micromole per Liter (umol/L)
variable NO3   float  
attribute NO3 _FillValue float NaN
attribute NO3 actual_range float 21.1, 22.3
attribute NO3 bcodmo_name String NO3
attribute NO3 colorBarMaximum double 50.0
attribute NO3 colorBarMinimum double 0.0
attribute NO3 description String Nitrate concentration
attribute NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute NO3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/ (external link)
attribute NO3 units String micromole per Liter (umol/L)
variable Si   short  
attribute Si _FillValue short 32767
attribute Si actual_range short 48, 158
attribute Si bcodmo_name String Si
attribute Si description String Silica concentration
attribute Si long_name String Mass Concentration Of Silicate In Sea Water
attribute Si units String micromole per Liter (umol/L)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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