ERDDAP > info > bcodmo_dataset_782308

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017		I   M	background			BCO-DMO	bcodmo_dataset_782308

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche (Thermal Performance Curve) was measured through a series of lab experiments and sequenced. Methods: Thermal niches were calculated by measuring each strain\u2019s thermal performance curve. This was done by acclimating aliquots of each culture for two weeks to a broad range of temperatures between 9\u00b0 and 33\u00b0. Temperatures >33\u00b0 were added as needed for strains able to grow at these levels.\u00a0 This temperature range was chosen because it exceeds the current summer high and low temperatures in Narragansett Bay, and encompasses the projected warmer temperatures expected in coming decades. Strains were grown at each temperature in triplicate 8 ml borosilicate vials containing 5ml of F/2 medium. Biomass was recorded every two days using in vivo chlorophyll a fluorescence measured on a Turner AU-10 fluorometer (Turner Designs Inc., Sunnyvale, CA, USA), and growth rates and Eppley-Norberg thermal performance curves (Norberg 2004) were calculated in R (Team 2019) using the package growthTools (DOI:10.5281/zenodo.3634918). Rare cultures containing contaminants (verified using fluorescence microscopy) were excluded from the dataset. In two strains for low temperature treatments, LA20 and LA27, after two weeks of acclimation no Synechococcus cells were observed in the culture, so the growth rate was set to zero for these cultures. The results of these thermal performance curves are hereafter referred to as a strain\u2019s phenotype. \u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	712792
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1638804
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638804
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Synechococcus thermal range The thermal niche for each of 11 new isolates of marine Synechococcus PI: D. Hutchins (USC) version date: 2019-11-20
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2019-11-21T13:36:54Z
attribute	NC_GLOBAL	date_modified	String	2020-04-27T20:45:01Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.782308.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/782308
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Turner Fluorometer 10-AU
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to measure fluorescence for growth rate calculations.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	783233
attribute	NC_GLOBAL	instruments_0_description	String	The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Turner Designs Fluorometer 10-AU
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	464
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Turner 10-AU Fluorometer (Turner Designs, CA)
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, isolate, management, maximum, minimum, oceanography, office, preliminary, rsqr, temperature, tmax, tmin, topt, width
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/782308/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/782308
attribute	NC_GLOBAL	param_mapping	String	{'782308': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/782308/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Southern California
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	USC
attribute	NC_GLOBAL	people_0_person_name	String	David A. Hutchins
attribute	NC_GLOBAL	people_0_person_nid	String	51048
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_1_person_nid	String	50396
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Phytoplankton Community Responses
attribute	NC_GLOBAL	projects_0_acronym	String	Phytoplankton Community Responses
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Photosynthetic marine microbes, phytoplankton, contribute half of global primary production, form the base of most aquatic food webs and are major players in global biogeochemical cycles. Understanding their community composition is important because it affects higher trophic levels, the cycling of energy and elements and is sensitive to global environmental change. This project will investigate how phytoplankton communities respond to two major global change stressors in aquatic systems: warming and changes in nutrient availability. The researchers will work in two marine systems with a long history of environmental monitoring, the temperate Narragansett Bay estuary in Rhode Island and a subtropical North Atlantic site near Bermuda. They will use field sampling and laboratory experiments with multiple species and varieties of phytoplankton to assess the diversity in their responses to different temperatures under high and low nutrient concentrations. If the diversity of responses is high within species, then that species may have a better chance to adapt to rising temperatures and persist in the future. Some species may already be able to grow at high temperatures; consequently, they may become more abundant as the ocean warms. The researchers will incorporate this response information in mathematical models to predict how phytoplankton assemblages would reorganize under future climate scenarios. Graduate students and postdoctoral associates will be trained in diverse scientific approaches and techniques such as shipboard sampling, laboratory experiments, genomic analyses and mathematical modeling. The results of the project will be incorporated into K-12 teaching, including an advanced placement environmental science class for underrepresented minorities in Los Angeles, data exercises for rural schools in Michigan and disseminated to the public through an environmental journalism institute based in Rhode Island. Predicting how ecological communities will respond to a changing environment requires knowledge of genetic, phylogenetic and functional diversity within and across species. This project will investigate how the interaction of phylogenetic, genetic and functional diversity in thermal traits within and across a broad range of species determines the responses of marine phytoplankton communities to rising temperature and changing nutrient regimes. High genetic and functional diversity within a species may allow evolutionary adaptation of that species to warming. If the phylogenetic and functional diversity is higher across species, species sorting and ecological community reorganization is likely. Different marine sites may have a different balance of genetic and functional diversity within and across species and, thus, different contribution of evolutionary and ecological responses to changing climate. The research will be conducted at two long-term time series sites in the Atlantic Ocean, the Narragansett Bay Long-Term Plankton Time Series and the Bermuda Atlantic Time Series (BATS) station. The goal is to assess intra- and inter-specific genetic and functional diversity in thermal responses at contrasting nutrient concentrations for a representative range of species in communities at the two sites in different seasons, and use this information to parameterize eco-evolutionary models embedded into biogeochemical ocean models to predict responses of phytoplankton communities to projected rising temperatures under realistic nutrient conditions. Model predictions will be informed by and tested with field data, including the long-term data series available for both sites and in community temperature manipulation experiments. This project will provide novel information on existing intraspecific genetic and functional thermal diversity for many ecologically and biogeochemically important phytoplankton species, estimate generation of new genetic and functional diversity in evolution experiments, and develop and parameterize novel eco-evolutionary models interfaced with ocean biogeochemical models to predict future phytoplankton community structure. The project will also characterize the interaction of two major global change stressors, warming and changing nutrient concentrations, as they affect phytoplankton diversity at functional, genetic, and phylogenetic levels. In addition, the project will develop novel modeling methodology that will be broadly applicable to understanding how other types of complex ecological communities may adapt to a rapidly warming world.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-09
attribute	NC_GLOBAL	projects_0_geolocation	String	Narragansett Bay, RI and Bermuda, Bermuda Atlantic Time-series Study (BATS)
attribute	NC_GLOBAL	projects_0_name	String	Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients
attribute	NC_GLOBAL	projects_0_project_nid	String	712787
attribute	NC_GLOBAL	projects_0_start_date	String	2016-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	The thermal niche, as calculated from the Thermal Performance Curve (TPC), for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017.
attribute	NC_GLOBAL	title	String	The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	Isolate		String
attribute	Isolate	bcodmo_name	String	taxon_code
attribute	Isolate	description	String	sample identifier; renamed from Sample_Code
attribute	Isolate	long_name	String	Isolate
attribute	Isolate	units	String	unitless
variable	topt		float
attribute	topt	_FillValue	float	NaN
attribute	topt	actual_range	float	24.4, 32.0
attribute	topt	bcodmo_name	String	temperature
attribute	topt	description	String	the temperature level to support fastest growth
attribute	topt	long_name	String	Topt
attribute	topt	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	topt	units	String	degrees Celsius
variable	tmin		float
attribute	tmin	_FillValue	float	NaN
attribute	tmin	actual_range	float	9.0, 19.1
attribute	tmin	bcodmo_name	String	temperature
attribute	tmin	description	String	the lowest temperature able to support growth
attribute	tmin	long_name	String	Minimum Temperature
attribute	tmin	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	tmin	units	String	degrees Celsius
variable	tmax		float
attribute	tmax	_FillValue	float	NaN
attribute	tmax	actual_range	float	33.5, 35.9
attribute	tmax	bcodmo_name	String	temperature
attribute	tmax	description	String	the highest temperature able to support growth
attribute	tmax	long_name	String	Maximum Temperature
attribute	tmax	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	tmax	units	String	degrees Celsius
variable	rsqr		float
attribute	rsqr	_FillValue	float	NaN
attribute	rsqr	actual_range	float	0.6, 1.0
attribute	rsqr	bcodmo_name	String	temperature
attribute	rsqr	description	String	R-square for thermal performance curve model
attribute	rsqr	long_name	String	RSQR
attribute	rsqr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	rsqr	units	String	unitless
variable	width		float
attribute	width	_FillValue	float	NaN
attribute	width	actual_range	float	16.1, 26.9
attribute	width	bcodmo_name	String	temperature
attribute	width	description	String	thermal niche width: range of temperatures able to support growth
attribute	width	long_name	String	Width
attribute	width	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	width	units	String	degrees Celsius

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.