ERDDAP > info > bcodmo_dataset_783679

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	Flow cytometry and nutrient analyses data from a tidal study over 48 hours of mangrove, seagrass, and seawater from the US Virgin Islands in July of 2017		I   M	background			BCO-DMO	bcodmo_dataset_783679

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute	NC_GLOBAL	acquisition_description	String	Materials and Methods Sampling. Sampling occurred from July 22-24, 2017 and coincided with the spring tides. A new moon occurred on July 23 at 5:45 (EST). While St. John tidal cycles are typically semidiurnal, during spring tide the tide cycle is diurnal. Sampling time points coincided with the low, flood, high, and ebb tides over a 48 hour (hr) window, resulting in 8 total sampling time points. Samples were collected \u00b11 hr from the designated time point, placed on ice, and processed within two hours of collection. Samples from nine locations across Lameshur Bay and Fish Bay on the south shore of St. John, U.S. Virgin Islands represented coral reef, seagrass bed and mangrove biomes. The Lameshur Bay mangrove location included two distinct sampling sites: an inland area that was only submerged and sampled during high tide and an outlet area that was always submerged and sampled at each time point. The majority of sampling sites were within the boundaries of the Virgin Islands National Park, which is largely undeveloped except for a small research station. The Fish Bay mangrove, Fish Bay seagrass, and Ditliff reef sites are outside the boundary of the park, and the land surrounding Fish Bay is inhabited. At all sites, a CTD (Castaway, SonTek, San Diego, CA, USA) was deployed from the surface to the bottom depth in reef and seagrass seawater, and single point measurements were collected from mangrove seawater, to capture the temperature and salinity at each timepoint over the course of the 48 hr sampling window. Only temperature and salinity at the surface of the cast was used for analysis. Samples for inorganic nutrients were collected by filling 30ml of surface seawater into acid-washed and seawater-rinsed vials (HDPE, Nalgene), followed by freezing to 20C. Seawater (875\u00b5l) was transferred to a 2ml cryovial (Corning) for analysis of microbial abundances and fixed to a final concentration of 1% paraformaldehyde (Electron Microscopy Sciences), allowed to fix for 20 min at 4C, then flash-frozen in an LN2 dry shipper. To capture seawater microbial communities, acid-washed 4L bottles (LDPE, Nalgene, ThermoFisher Scientific, Waltham, MA, USA) were rinsed three times with seawater prior to collection of 3L of surface seawater. The specific 4L bottle used for a site at the first timepoint remained consistent across all sampling timepoints, and the bottles were rinsed with freshwater between uses. Following collection, 1L of seawater was pumped using a Masterflex peristaltic pump (Cole-Palmer, Vernon Hills, IL, USA) through Masterflex silicone tubing (L/S, platinum-cured, #96410-24 size, Cole-Parmer) to rinse the tubing. The remaining 2L of seawater was filtered through a 0.22\u00b5m Supor filter (25mm; Pall, Ann Arbor, MI, USA). For the mangrove and seagrass sites, 2L could not always be filtered completely and therefore 0.3 - 2L and 1.2 - 2L of water was filtered through the membrane, respectively. For the coral reef sites, 1.5 - 2L passed through the filter membrane. All filters were placed into 2ml cryovials (Corning, Corning, NY, USA) and flash-frozen in a liquid nitrogen dry shipper until returned to Woods Hole, MA and placed at 80C. Flow cytometry and Nutrient analyses. Samples for microbial abundance were analyzed at the University of Hawaii with an EPICS Altra flow cytometry (Beckman Coulter Life Sciences, Inc, Indianapolis, IN) as described in Furby et al (Furby et al. 2014), with some modifications. Briefly, to obtain concentrations of cyanobacteria (Prochlorococcus and Synechococcus) and eukaryotic phytoplankton (picoeukaryotes), an unstained aliquot was run on the instrument and excited by visible wavelengths. To enumerate unpigmented cells, which is a proxy for heterotrophic bacteria and archaea (Marie et al. 1997), an aliquot was stained with a Hoechst DNA stain and run on the flow cytometer with excitation at 488nm. The number of cells per ml was estimated following analysis of fluorescence spectra using FlowJo software (v 6.4.7, Tree Star, Inc., Ashland, OR, USA). Samples for nutrient analysis were analyzed at Oregon State University using methods described in Furby et al. (Furby et al. 2014) to measure dissolved concentrations (\u00b5M) of phosphate, ammonium, nitrite, nitrite + nitrate, and silicate. DNA Extraction, PCR amplification, and Sequencing. DNA was extracted from the filters using a sucrose-EDTA lysis method similar to Santoro et al. (Santoro et al. 2010) that combines lysis with filter column purification. Briefly, the 25mm filter was subjected to physical and chemical lysis using 0.1mm glass beads (Lysing Matrix B, MP Biomedicals, Irvine, CA, USA), sucrose-EDTA lysis buffer (0.75M Sucrose, 20mM EDTA, 400mM NaCl, 50 mM Tris) and 10% sodium dodecyl sulfate (Teknova, Hollister, CA, USA), followed by a proteinase-K digestion (20 mg/ml Promega, Madison, WI, USA). Lysate was then purified using the DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD, USA) spin column filters. Purified DNA was fluorometrically quantified using a high sensitivity dsDNA assay on a Qubit 2.0 fluorometer (ThermoFisher Scientific). Sample DNA was diluted 1:100 in UV-sterilized PCR-grade H2O and 1\u00b5l was used in a PCR reaction. Barcoded primers recommended by the Earth Microbiome Project, 515FY and 806RB, were used to amplify the V4 region of the SSU rRNA gene in bacteria and archaea (Apprill et al. 2015, Parada et al. 2016). Triplicate 25\u00b5l reactions contained 1.25 units of GoTaq DNA Polymerase (Promega, Madison, WI, USA), 0.2\u00b5M forward and reverse primers, 0.2mM deoxynucleoside triphosphate (dNTP) mix (Promega), 2.5mM MgCl2, 5\u00b5l GoTaq 5X colorless flexi buffer (Promega), and nuclease-free water. The reactions were run on a Bio-Rad Thermocycler (Hercules, CA, USA) using the following criteria: denaturation at 95C for 2 min; 28C cycles of 95C for 20 s, 55C for 15 s, and 72C for 5 min; and extension at 72C for 10 min. Successful amplification was verified by running 5\u00b5l of product on a 1% agarose-TBE gel stained with SYBR Safe gel stain (Invitrogen, Carlsbad, CA, USA). Triplicate PCR products were pooled and purified using the MinElute PCR purification kit (Qiagen). Concentration of purified products was quantified using the high sensitivity dsDNA assay on the Qubit 2.0 fluorometer (ThermoFisher Scientific). Barcoded PCR products were diluted to equal concentrations and pooled for sequencing. Samples were shipped to the Georgia Genomics and Bioinformatics Core at the University of Georgia for sequencing on an Illumina MiSeq using paired-end 250bp sequencing. Data analysis. All sequence processing and data analysis was performed in R Studio (v 1.1.463) running R (v 3.4.0, 2017-04-21). Sequence reads were inspected for quality, filtered, trimmed, and dereplicated in the DADA2 R package (v.1.10.0) (Callahan et al. 2016). Specific filtering parameters used included the following: truncLen = c(240, 200), maxN = 0, maxEE = c(2,2), rm.phix = TRUE, and compress = TRUE. DADA2 was then used to generate amplicon sequence variants (ASVs) and remove chimeras. Taxonomy was assigned in DADA2 using the SILVA SSU rRNA database down to the species level where applicable (v.132, (Quast et al. 2012)). ASV counts in each sample were transformed to relative abundance for further data analysis. To understand the variability in microbial communities over time at all sites, Bray-Curtis dissimilarity was calculated between each sample in the R package vegan (v2.5.4) (Oksanen et al. 2019) and illustrated using non-metric multidimensional scaling (NMDS) in the R package, ggplot2 (v3.2.1) (Wickham 2016). Environmental vectors that significantly associated (cutoff p<0.01) with the ordination were produced using the function envfit() in the vegan R package. Pairwise dissimilarity was plotted to represent the range of dissimilarity in microbial communities over 48 hrs at each site. A higher average dissimilarity would suggest that the site experiences more variable microbial communities than a site with a lower average dissimilarity. A Kruskal-Wallis test was used to examine if there is a significant difference between sites (significance level p<0.05). To determine which pairs of sites had significantly different dissimilarities, a pairwise Wilcoxon Rank Sum test was used with a Benjamini-Hochberg correction for multiple testing and a cutoff of 0.05. Differential abundance (DA) and of ASVs in relation to the tide was evaluated at mangrove sites using the corncob R package (v0.1.0) (Martin et al. 2019). The following analyses were conducted on a subset of the data representing mangrove communities. All ASV relative abundances were modeled in corncob using a logit-link for mean and dispersion. DA was modeled as a linear function of sea level (a continuous covariate that is representative of the tide cycle) while controlling for differential variance and the effect of site and day or night on DA. Controlling for the effect of day or night was imperative because over the 48 hr period low and flood tide only occurred during the day and high and low tide only occurred during dusk and night, respectively. The parametric Wald test was used to test the hypotheses that the relative abundance or variance of a given ASV changed significantly with respect to sea level and the Benjamini-Hochberg false discovery rate (FDR) correction was applied to account for multiple comparisons, with the cutoff at 0.05.
attribute	NC_GLOBAL	awards_0_award_nid	String	746195
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1736288
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736288
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Daniel Thornhill
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	722161
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Tidal study of seawater microbial communities PI: Amy Apprill Data Version 1: 2019-12-09
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-12-06T16:55:52Z
attribute	NC_GLOBAL	date_modified	String	2019-12-12T20:24:25Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.783679.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-64.72223
attribute	NC_GLOBAL	geospatial_lat_max	double	18.32065
attribute	NC_GLOBAL	geospatial_lat_min	double	18.30964
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-64.72223
attribute	NC_GLOBAL	geospatial_lon_min	double	-64.76453
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	0.3
attribute	NC_GLOBAL	geospatial_vertical_min	double	0.3
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/783679
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	CTD
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	783835
attribute	NC_GLOBAL	instruments_0_description	String	The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/130/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	CTD profiler
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	417
attribute	NC_GLOBAL	instruments_0_supplied_name	String	CTD (Castaway, SonTek, San Diego, CA, USA)
attribute	NC_GLOBAL	instruments_1_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	783845
attribute	NC_GLOBAL	instruments_1_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Qubit 2.0 fluorometer (ThermoFisher Scientific)
attribute	NC_GLOBAL	instruments_2_acronym	String	Automated Sequencer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Samples were shipped to the Georgia Genomics and Bioinformatics Core at the University of Georgia for sequencing on an Illumina MiSeq using paired-end 250bp sequencing
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	783846
attribute	NC_GLOBAL	instruments_2_description	String	General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Automated DNA Sequencer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	649
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Illumina MiSeq
attribute	NC_GLOBAL	instruments_3_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	783836
attribute	NC_GLOBAL	instruments_3_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_3_supplied_name	String	EPICS Altra flow cytometry (Beckman Coulter Life Sciences, Inc, Indianapolis, IN)
attribute	NC_GLOBAL	keywords	String	accession, ammonia, ammonium, bco, bco-dmo, bio, biological, biome, cells, chemical, chemistry, collection, Collection_Date, Collection_Depth, Collection_Time, concentration, data, dataset, date, density, depth, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Chemistry > Phosphate, Earth Science > Oceans > Ocean Chemistry > Silicate, Earth Science > Oceans > Salinity/Density > Salinity, Earth Science > Oceans > Sea Surface Topography > Sea Surface Height, erddap, height, instrument, iso, latitude, longitude, management, mass, mass_concentration_of_phosphate_in_sea_water, mass_concentration_of_silicate_in_sea_water, model, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrate_in_sea_water, mole_concentration_of_nitrite_in_sea_water, n02, name, ncbi, NCBI_BioProject_accession_number, NCBI_BioSample_accession_number, nh4, nitrate, Nitrate_Nitrite, nitrite, no3, number, ocean, oceanography, oceans, office, phosphate, picoeukaryotes, po4, practical, preliminary, prochlorococcus, project, salinity, sample, Sample_ID, Sample_type, science, sea, sea_surface_height, sea_water_practical_salinity, seawater, sequencing, Sequencing_Instrument_model, Sequencing_Strategy, Sequencing_strategy, silicate, site, Site_Depth, Site_Name, strategy, surface, synechococcus, temperature, tide, Tide_Height, time, Time_elapsed_between_tide_timepoint_and_collection, topography, type, unpigmented, Unpigmented_cells, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/783679/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/783679
attribute	NC_GLOBAL	Northernmost_Northing	double	18.32065
attribute	NC_GLOBAL	param_mapping	String	{'783679': {'Latitude': 'master - latitude', 'Collection_Depth': 'master - depth', 'Longitude': 'master - longitude', 'ISO_DateTime_UTC': 'master - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/783679/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Amy Apprill
attribute	NC_GLOBAL	people_0_person_nid	String	553489
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_1_person_name	String	Cynthia Becker
attribute	NC_GLOBAL	people_1_person_nid	String	784315
attribute	NC_GLOBAL	people_1_role	String	Student
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Amber York
attribute	NC_GLOBAL	people_2_person_nid	String	643627
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	Coral Exometabolomes
attribute	NC_GLOBAL	projects_0_acronym	String	Coral Exometabolomes
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: Coral reefs are some of the most diverse and productive ecosystems in the ocean. Globally, reefs have declined in stony (reef-building) coral abundance due to environmental variations, and in the Caribbean this decline has coincided with an increase in octocoral (soft coral) abundance. This phase shift occurring on Caribbean reefs may be impacting the interactions between the sea floor and water column and particularly between corals and picoplankton. Picoplankton are the microorganisms in the water column that utilize organic matter released from corals to support their growth. These coral-picoplankton interactions are relatively unstudied, but could have major implications for reef ecology and coral health. This project will take place in the U.S. territory of the Virgin Islands (USVI) and will produce the first detailed knowledge about the chemical diversity and composition of organic matter released from diverse stony coral and octocoral species. This project will advance our understanding of coral reef microbial ecology by allowing us to understand how different coral metabolites impact picoplankton growth and dynamics over time. The results from this project will be made publically accessible in a freely available online magazine, and USVI minority middle and high school students will be exposed to a lesson about chemical-biological interactions on coral reefs through established summer camps. This project will also contribute to the training of USVI minority undergraduates as well as a graduate student. Coral exometabolomes, which are the sum of metabolic products of the coral together with its microbiome, are thought to structure picoplankton communities in a species-specific manner. However, a detailed understanding of coral exometabolomes, and their influences on reef picoplankton, has not yet been obtained. This project will utilize controlled aquaria-based experiments with stony corals and octocorals, foundational species of Caribbean reef ecosystems, to examine how the exometabolomes of diverse coral species differentially influence the reef picoplankton community. Specifically, this project will capitalize on recent developments in mass spectrometry-based metabolomics to define the signature exometabolomes of ecologically important and diverse stony corals and octocorals. Secondly, this project will determine how the exometabolomes of these corals vary with factors linked to coral taxonomy as well as the coral-associated microbiome (Symbiodinium algae, bacteria and archaea). With this new understanding of coral exometabolomes, the project will then apply a stable isotope probe labeling approach to the coral exometabolome and will examine if and how (through changes in growth and activity) the seawater picoplankton community incorporates coral exometabolomes from different coral species over time. This project will advance our ability to evaluate the role that coral exometabolomes play in contributing to benthic-picoplankton interactions on changing Caribbean reefs.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-09
attribute	NC_GLOBAL	projects_0_geolocation	String	U.S. Virgin Islands
attribute	NC_GLOBAL	projects_0_name	String	Signature exometabolomes of Caribbean corals and influences on reef picoplankton
attribute	NC_GLOBAL	projects_0_project_nid	String	746196
attribute	NC_GLOBAL	projects_0_start_date	String	2017-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	18.30964
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	NCBI_BioProject_accession_number,Sample_type,Sequencing_Strategy,Sequencing_Instrument_model
attribute	NC_GLOBAL	summary	String	Data from a tidal study over 48 hours of mangrove, seagrass, and seawater from the US Virgin Islands in 2017. These data include tidal height, depth, temperature, salinity, Prochlorococcus counts, Synechococcus counts, Picoeukaryote abundances, nutrient concentrations at accession numbers for sequences at The National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA).
attribute	NC_GLOBAL	time_coverage_end	String	2017-07-24T10:43:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2017-07-22T15:55:00Z
attribute	NC_GLOBAL	title	String	Flow cytometry and nutrient analyses data from a tidal study over 48 hours of mangrove, seagrass, and seawater from the US Virgin Islands in July of 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-64.76453
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Sample_ID		String
attribute	Sample_ID	bcodmo_name	String	sample
attribute	Sample_ID	description	String	Sample identifier
attribute	Sample_ID	long_name	String	Sample ID
attribute	Sample_ID	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Sample_ID	units	String	unitless
variable	NCBI_BioProject_accession_number		String
attribute	NCBI_BioProject_accession_number	bcodmo_name	String	accession number
attribute	NCBI_BioProject_accession_number	description	String	BioProject accession number for the NCBI Sequence read archive
attribute	NCBI_BioProject_accession_number	long_name	String	NCBI Bio Project Accession Number
attribute	NCBI_BioProject_accession_number	units	String	unitless
variable	NCBI_BioSample_accession_number		String
attribute	NCBI_BioSample_accession_number	bcodmo_name	String	accession number
attribute	NCBI_BioSample_accession_number	description	String	BioSample accession number for the NCBI Sequence read archive
attribute	NCBI_BioSample_accession_number	long_name	String	NCBI Bio Sample Accession Number
attribute	NCBI_BioSample_accession_number	units	String	unitless
variable	Sample_type		String
attribute	Sample_type	bcodmo_name	String	sample_descrip
attribute	Sample_type	description	String	Sample type as reported to NCBI for sequence upload
attribute	Sample_type	long_name	String	Sample Type
attribute	Sample_type	units	String	unitless
variable	Sequencing_Strategy		String
attribute	Sequencing_Strategy	bcodmo_name	String	brief_desc
attribute	Sequencing_Strategy	description	String	Describes what type of sequencing library preparation we did
attribute	Sequencing_Strategy	long_name	String	Sequencing Strategy
attribute	Sequencing_Strategy	units	String	unitless
variable	Sequencing_Instrument_model		String
attribute	Sequencing_Instrument_model	bcodmo_name	String	brief_desc
attribute	Sequencing_Instrument_model	description	String	This is the model type of sequencer used for the dataset uploaded to NCBI
attribute	Sequencing_Instrument_model	long_name	String	Sequencing Instrument Model
attribute	Sequencing_Instrument_model	units	String	unitless
variable	Sequencing_strategy		String
attribute	Sequencing_strategy	bcodmo_name	String	brief_desc
attribute	Sequencing_strategy	description	String	This is a more detailed description of how we sequenced. This is included in the methods in more depth.
attribute	Sequencing_strategy	long_name	String	Sequencing Strategy
attribute	Sequencing_strategy	units	String	unitless
variable	Site_Name		String
attribute	Site_Name	bcodmo_name	String	site
attribute	Site_Name	description	String	This is the local name of the site where samples were collected
attribute	Site_Name	long_name	String	Site Name
attribute	Site_Name	units	String	unitless
variable	Biome		String
attribute	Biome	bcodmo_name	String	site_descrip
attribute	Biome	description	String	This is a qualitative assessment of the type of ecosystem
attribute	Biome	long_name	String	Biome
attribute	Biome	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	18.30964, 18.32065
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-64.76453, -64.72223
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	Collection_Date		String
attribute	Collection_Date	bcodmo_name	String	date_local
attribute	Collection_Date	description	String	Local date when samples were collected (UTC-4)
attribute	Collection_Date	long_name	String	Collection Date
attribute	Collection_Date	time_precision	String	1970-01-01
attribute	Collection_Date	units	String	unitless
variable	Collection_Time		String
attribute	Collection_Time	bcodmo_name	String	time_local
attribute	Collection_Time	description	String	Local time when samples were collected (UTC-4)
attribute	Collection_Time	long_name	String	Collection Time
attribute	Collection_Time	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.5007389E9, 1.50089298E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	ISO Datetime (UTC) when samples were collected in ISO 8601:2004(E) format yyyy-mm-ddTHH:MM:SSZ
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	Tide		String
attribute	Tide	bcodmo_name	String	tide
attribute	Tide	description	String	Qualitative description of the tide level during the sample timepoint
attribute	Tide	long_name	String	Tide
attribute	Tide	units	String	unitless
variable	Tide_Height		float
attribute	Tide_Height	_FillValue	float	NaN
attribute	Tide_Height	actual_range	float	-0.042, 0.401
attribute	Tide_Height	bcodmo_name	String	height
attribute	Tide_Height	colorBarMaximum	double	2.0
attribute	Tide_Height	colorBarMinimum	double	-2.0
attribute	Tide_Height	description	String	Tide height corresponding to verified mean low low water (MLLW), as taken from the NOAA station 9751381 in Lameshur Bay, USVI.
attribute	Tide_Height	long_name	String	Sea Surface Height
attribute	Tide_Height	units	String	meters (m)
variable	Time_elapsed_between_tide_timepoint_and_collection		byte
attribute	Time_elapsed_between_tide_timepoint_and_collection	_FillValue	byte	127
attribute	Time_elapsed_between_tide_timepoint_and_collection	actual_range	byte	0, 58
attribute	Time_elapsed_between_tide_timepoint_and_collection	bcodmo_name	String	time_elapsed
attribute	Time_elapsed_between_tide_timepoint_and_collection	colorBarMaximum	double	2.0
attribute	Time_elapsed_between_tide_timepoint_and_collection	colorBarMinimum	double	-2.0
attribute	Time_elapsed_between_tide_timepoint_and_collection	description	String	Time that elapsed between the designated collection timepoint and when the sample was actually collected during the time series.
attribute	Time_elapsed_between_tide_timepoint_and_collection	long_name	String	Sea Surface Height
attribute	Time_elapsed_between_tide_timepoint_and_collection	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	Time_elapsed_between_tide_timepoint_and_collection	units	String	minutes
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	0.3, 0.3
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	description	String	Collection depth of all samples
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Collection Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	Site_Depth		float
attribute	Site_Depth	_FillValue	float	NaN
attribute	Site_Depth	actual_range	float	0.2, 12.2
attribute	Site_Depth	bcodmo_name	String	depth
attribute	Site_Depth	description	String	Approximate depth of the collection site. Generally taken from the CTD cast.
attribute	Site_Depth	long_name	String	Site Depth
attribute	Site_Depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	Site_Depth	units	String	meters (m)
variable	Temperature		float
attribute	Temperature	_FillValue	float	NaN
attribute	Temperature	actual_range	float	27.84, 34.4
attribute	Temperature	bcodmo_name	String	temperature
attribute	Temperature	description	String	Temperature of seawater as taken from the CastAway CTD (SonTek)
attribute	Temperature	long_name	String	Temperature
attribute	Temperature	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	Temperature	units	String	degrees Celsius (ºC)
variable	Salinity		float
attribute	Salinity	_FillValue	float	NaN
attribute	Salinity	actual_range	float	34.52, 37.38
attribute	Salinity	bcodmo_name	String	sal
attribute	Salinity	colorBarMaximum	double	37.0
attribute	Salinity	colorBarMinimum	double	32.0
attribute	Salinity	description	String	Salinity of seawater as taken from the CastAway CTD (SonTek)
attribute	Salinity	long_name	String	Sea Water Practical Salinity
attribute	Salinity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/
attribute	Salinity	units	String	Practical Salinity Units (PSU)
variable	Prochlorococcus		int
attribute	Prochlorococcus	_FillValue	int	2147483647
attribute	Prochlorococcus	actual_range	int	0, 197086
attribute	Prochlorococcus	bcodmo_name	String	cell_concentration
attribute	Prochlorococcus	description	String	Prochlorococcus cell counts as determined by flow cytometry
attribute	Prochlorococcus	long_name	String	Prochlorococcus
attribute	Prochlorococcus	units	String	cells per milliliter (cells/ml)
variable	Synechococcus		int
attribute	Synechococcus	_FillValue	int	2147483647
attribute	Synechococcus	actual_range	int	1547, 95963
attribute	Synechococcus	bcodmo_name	String	cell_concentration
attribute	Synechococcus	description	String	Synechococcus cell counts as determined by flow cytometry
attribute	Synechococcus	long_name	String	Synechococcus
attribute	Synechococcus	units	String	cells per milliliter (cells/ml)
variable	Picoeukaryotes		short
attribute	Picoeukaryotes	_FillValue	short	32767
attribute	Picoeukaryotes	actual_range	short	844, 6441
attribute	Picoeukaryotes	bcodmo_name	String	cell_concentration
attribute	Picoeukaryotes	description	String	Picoeukaryote abundances as determined by flow cytometry
attribute	Picoeukaryotes	long_name	String	Picoeukaryotes
attribute	Picoeukaryotes	units	String	cells per milliliter (cells/ml)
variable	Unpigmented_cells		int
attribute	Unpigmented_cells	_FillValue	int	2147483647
attribute	Unpigmented_cells	actual_range	int	366483, 4500000
attribute	Unpigmented_cells	bcodmo_name	String	cell_concentration
attribute	Unpigmented_cells	description	String	unpigmented cells as determined by flow cytometry of Hoescht stained cells.
attribute	Unpigmented_cells	long_name	String	Unpigmented Cells
attribute	Unpigmented_cells	units	String	cells per milliliter (cells/ml)
variable	Phosphate		float
attribute	Phosphate	_FillValue	float	NaN
attribute	Phosphate	actual_range	float	0.157, 0.553
attribute	Phosphate	bcodmo_name	String	PO4
attribute	Phosphate	description	String	Phosphate concentrations in seawater
attribute	Phosphate	long_name	String	Mass Concentration Of Phosphate In Sea Water
attribute	Phosphate	units	String	micromolar (µM)
variable	Silicate		float
attribute	Silicate	_FillValue	float	NaN
attribute	Silicate	actual_range	float	1.7, 9.1
attribute	Silicate	bcodmo_name	String	SiOH_4
attribute	Silicate	description	String	Silicate concentrations in seawater
attribute	Silicate	long_name	String	Mass Concentration Of Silicate In Sea Water
attribute	Silicate	units	String	micromolar (µM)
variable	Nitrate_Nitrite		float
attribute	Nitrate_Nitrite	_FillValue	float	NaN
attribute	Nitrate_Nitrite	actual_range	float	-0.01, 1.17
attribute	Nitrate_Nitrite	bcodmo_name	String	NO3_NO2
attribute	Nitrate_Nitrite	colorBarMaximum	double	50.0
attribute	Nitrate_Nitrite	colorBarMinimum	double	0.0
attribute	Nitrate_Nitrite	description	String	Nitrate and Nitrite concentrations in seawater
attribute	Nitrate_Nitrite	long_name	String	Mole Concentration Of Nitrate In Sea Water
attribute	Nitrate_Nitrite	units	String	micromolar (µM)
variable	Nitrite		float
attribute	Nitrite	_FillValue	float	NaN
attribute	Nitrite	actual_range	float	0.01, 0.26
attribute	Nitrite	bcodmo_name	String	NO2
attribute	Nitrite	colorBarMaximum	double	1.0
attribute	Nitrite	colorBarMinimum	double	0.0
attribute	Nitrite	description	String	Nitrite concentrations in seawater
attribute	Nitrite	long_name	String	Mole Concentration Of Nitrite In Sea Water
attribute	Nitrite	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/
attribute	Nitrite	units	String	micromolar (µM)
variable	Ammonium		float
attribute	Ammonium	_FillValue	float	NaN
attribute	Ammonium	actual_range	float	0.02, 2.94
attribute	Ammonium	bcodmo_name	String	Ammonium
attribute	Ammonium	colorBarMaximum	double	5.0
attribute	Ammonium	colorBarMinimum	double	0.0
attribute	Ammonium	description	String	Ammonium concentrations in seawater
attribute	Ammonium	long_name	String	Mole Concentration Of Ammonium In Sea Water
attribute	Ammonium	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/
attribute	Ammonium	units	String	micromolar (µM)

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