ERDDAP > info > bcodmo_dataset_787093

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Trichodesmiium field metaproteomes - sample provenance] - Trichodesmium sample provenance from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 2018 (Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean)		F   I   M	background			BCO-DMO	bcodmo_dataset_787093

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	A 200mm plankton net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand-picked into 0.2mm filtered surface seawater, rinsed twice in 0.2mm filtered surface seawater, and decanted onto a 0.2-4mm Supor filter.
attribute	NC_GLOBAL	awards_0_award_nid	String	646121
attribute	NC_GLOBAL	awards_0_award_number	String	GBMF3934
attribute	NC_GLOBAL	awards_0_funder_name	String	Gordon and Betty Moore Foundation
attribute	NC_GLOBAL	awards_0_funding_acronym	String	GBMF
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	361
attribute	NC_GLOBAL	awards_1_award_nid	String	646122
attribute	NC_GLOBAL	awards_1_award_number	String	GBMF3782
attribute	NC_GLOBAL	awards_1_data_url	String	https://www.moore.org/grant-detail?grantId=GBMF3782
attribute	NC_GLOBAL	awards_1_funder_name	String	Gordon and Betty Moore Foundation: Marine Microbiology Initiative
attribute	NC_GLOBAL	awards_1_funding_acronym	String	MMI
attribute	NC_GLOBAL	awards_1_funding_source_nid	String	385
attribute	NC_GLOBAL	awards_2_award_nid	String	724457
attribute	NC_GLOBAL	awards_2_award_number	String	OCE-1657766
attribute	NC_GLOBAL	awards_2_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1657766
attribute	NC_GLOBAL	awards_2_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_2_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_2_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_2_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_2_program_manager_nid	String	50534
attribute	NC_GLOBAL	awards_3_award_nid	String	786678
attribute	NC_GLOBAL	awards_3_award_number	String	OCE-1850719
attribute	NC_GLOBAL	awards_3_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1850719
attribute	NC_GLOBAL	awards_3_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_3_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_3_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_3_program_manager	String	Daniel Thornhill
attribute	NC_GLOBAL	awards_3_program_manager_nid	String	722161
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Trichodesmium sample provenance PI: Mak A. Saito Data Version 1: 2020-01-10
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2020-01-10T20:04:13Z
attribute	NC_GLOBAL	date_modified	String	2020-04-02T18:12:49Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.787093.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-21.59
attribute	NC_GLOBAL	geospatial_lat_max	double	31.4
attribute	NC_GLOBAL	geospatial_lat_min	double	0.17976
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-21.59
attribute	NC_GLOBAL	geospatial_lon_min	double	-158.0
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/787093
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, cruise, Cruise_station_num, data, dataset, date, dmo, erddap, filter, Filter_size, latitude, longitude, management, name, net, Net_size, num, oceanography, office, preliminary, replication, sampled, size, station, time, Time_sampled, time_zone, zone
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/787093/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/787093
attribute	NC_GLOBAL	Northernmost_Northing	double	31.4
attribute	NC_GLOBAL	param_mapping	String	{'787093': {'Latitude': 'master - latitude', 'Longitude': 'master - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/787093/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Mak A. Saito
attribute	NC_GLOBAL	people_0_person_nid	String	50985
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_1_person_name	String	Noelle Held
attribute	NC_GLOBAL	people_1_person_nid	String	787371
attribute	NC_GLOBAL	people_1_role	String	Student
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Amber York
attribute	NC_GLOBAL	people_2_person_nid	String	643627
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	TriCoLim,MM Proteins and Organics Tech,MM Saito,Cyanobacteria Warming Responses
attribute	NC_GLOBAL	projects_0_acronym	String	TriCoLim
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: Marine cyanobacteria are able to use or "fix" atmospheric nitrogen gas, and so supply much of the essential nutrient nitrogen that supports open ocean food chains. Oceanographers have usually thought that the growth of these nitrogen-fixing cyanobacteria is limited at any particular time and place by the supply of either iron, or of phosphorus. Preliminary experiments have shown, though, that these nitrogen fixers instead grow best when both iron and phosphorus are scarce at the same time. In this project, the researchers will use cellular indicators that are specific for iron and phosphorus limitation to determine how important this type of "balanced limitation" of nitrogen-fixing cyanobacteria is in controlling the productivity of ocean food chains in the tropical Atlantic Ocean. Two graduate students will be trained at the University of Southern California (USC) and Woods Hole Oceanographic Institution, as well as a postdoctoral researcher at USC. Educational outreach efforts will take place at a Los Angeles inner city high school with a student body that is over 98% Hispanic and African-American, and with underrepresented undergraduates in the USC Global Environmental Microbiology course. In addition, two Research Experiences for Undergraduates students will be supervised for summer research projects to help them learn about science career options. The researchers will investigate the biological and biogeochemical consequences of this unique balanced iron/phosphorus-limited phenotype, using both laboratory and fieldwork approaches. During the first year of this project, the nitrogen-fixing cyanobacteria will be cultured under iron and/or phosphorus limitation, followed by application of proteomics and transcriptomics to identify genes that are potential diagnostic biomarkers for iron/phosphorus balanced limitation. Preliminary work has already identified one promising candidate biomarker in one cyanobacterium, an EzrA protein domain that appears to be associated with the cell size decreases seen specifically under balanced limitation, and the researchers have identified numerous other potential candidates for similar biomarkers. During the second year, these new co-limitation biomarkers and others previously validated for iron limitation (IsiB) and phosphorus limitation (SphX) will be used to investigate balanced limitation during a research cruise transecting from relatively high-iron, low-phosphorus North Atlantic waters, to the relatively high-phosphorus, low-iron South Atlantic. This fieldwork component will survey nitrogen fixing cyanobacteria populations across this natural iron/phosphorus gradient for genetic, proteomic, and physiological indicators of balanced limitation, as well as testing their responses to iron and phosphorus manipulations in shipboard incubation experiments. The third year will be devoted to sample analysis, and publications exploring the responses of oceanic nitrogen fixers to simultaneous limitation by both iron and phosphorus.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-02
attribute	NC_GLOBAL	projects_0_geolocation	String	Tropical Atlantic
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
attribute	NC_GLOBAL	projects_0_project_nid	String	724451
attribute	NC_GLOBAL	projects_0_start_date	String	2017-03
attribute	NC_GLOBAL	projects_1_acronym	String	MM Proteins and Organics Tech
attribute	NC_GLOBAL	projects_1_description	String	In support of acquiring a high resolution mass spectrometer that incorporates the latest technologies for analyzing proteins and other organic materials.
attribute	NC_GLOBAL	projects_1_end_date	String	2014-06
attribute	NC_GLOBAL	projects_1_name	String	New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms
attribute	NC_GLOBAL	projects_1_project_nid	String	786670
attribute	NC_GLOBAL	projects_1_project_website	String	https://www.moore.org/grant-detail?grantId=GBMF3934
attribute	NC_GLOBAL	projects_1_start_date	String	2013-06
attribute	NC_GLOBAL	projects_2_acronym	String	MM Saito
attribute	NC_GLOBAL	projects_2_description	String	In support of obtaining deeper knowledge of major biogeochemically relevant proteins to inform a mechanistic understanding of global marine biogeochemical cycles.
attribute	NC_GLOBAL	projects_2_end_date	String	2019-12
attribute	NC_GLOBAL	projects_2_name	String	Marine Microbial Investigator Award: Investigator Mak Saito
attribute	NC_GLOBAL	projects_2_project_nid	String	786672
attribute	NC_GLOBAL	projects_2_start_date	String	2013-05
attribute	NC_GLOBAL	projects_3_acronym	String	Cyanobacteria Warming Responses
attribute	NC_GLOBAL	projects_3_description	String	NSF abstract: The oceans absorb much of the heat generated by human activities, and this warming of the surface ocean has consequences for important groups of marine organisms. Marine cyanobacteria are one such key group of organisms, since they supply much of the essential carbon and nitrogen that supports nearly all the rest of the marine food web. Currently, the growth of cyanobacteria is mostly constrained by scarce supplies of the micronutrient element iron, but they are also very sensitive to the ongoing increases in seawater temperature. Preliminary results suggest that warming could partly mitigate the negative effects of iron limitation on marine cyanobacteria. This project examines in depth how these interactions between warming and iron limitation will affect the future ocean carbon and nitrogen cycles, using laboratory culture experiments showing how cyanobacteria respond to simultaneously changing temperature and iron supplies. Both short-term response studies and long-term evolutionary experiments testing for adaptation use a comprehensive set of molecular biology tools targeting genes to proteins. The final goal is to apply the results of these experiments to improve quantitative models predicting how the ocean's carbon and nitrogen cycles, biological productivity, and living resources will respond to a warming future climate. Two graduate students, a postdoc and 3-4 underrepresented undergraduate researchers are supported, and the investigators also mentor summer science interns from largely Hispanic local high schools. The physiology, biochemistry and biogeography of nitrogen-fixing cyanobacteria and unicellular picocyanobacteria are strongly influenced by temperature, subjecting them to intense selective pressure as the modern ocean steadily warms up. These groups have likewise been rigorously selected under chronic iron (Fe) scarcity, and the availability of this crucial micronutrient is also changing with a shifting climate. This project examines short-term acclimation and long-term evolutionary responses of Fe-stressed marine cyanobacteria to a warmer environment. Preliminary data show that Iron Use Efficiencies (IUE, mols N fixed.hr-1 mol cellular Fe-1) of Fe-limited Trichodesmium increase 4 to 5-fold with a 5oC temperature increase, allowing the cells to much more efficiently leverage scarce available Fe supplies to grow and fix nitrogen. This means that warming can to a large degree mitigate the negative effects of Fe limitation on Trichodesmium, resulting in a modelled 22% increase in global nitrogen fixation by 2100 in a warmer climate. This project aims to uncover the cellular biochemical mechanisms involved in this Fe-limitation/thermal IUE effect in a four-year experimental evolution study of the diazotrophs Trichodesmium and Crocosphaera and the picocyanobacteria Synechococcus and Prochlorococcus, under a multi-variate selection matrix of temperature and Fe availability. The objectives are to 1) Assess the long-term adaptive responses of fitness, IUE and physiology to Fe limitation and warming interactions in these four major cyanobacterial groups; 2) Determine the molecular and biochemical mechanisms behind the surprising Fe/warming interactive effect on IUE using genomics, transcriptomics and quantitative proteomics coupled with 'metalloproteomics' determinations of Fe content in critical proteins; 3) Compare and contrast acclimation and adaptation responses to Fe limitation and warming in key cyanobacteria taxa, and 4) Integrate results using a published biogeochemical modeling approach to assess global consequences for marine productivity and nitrogen fixation. This project offers a mechanistic and predictive understanding of adaptation to Fe and warming co-stressors in a rapidly changing future ocean environment for some of the most important photoautotrophic functional groups in the ocean. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
attribute	NC_GLOBAL	projects_3_end_date	String	2023-03
attribute	NC_GLOBAL	projects_3_name	String	Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions
attribute	NC_GLOBAL	projects_3_project_nid	String	786679
attribute	NC_GLOBAL	projects_3_start_date	String	2019-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	0.17976
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Trichodesmium sample provenance collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.
attribute	NC_GLOBAL	title	String	[Trichodesmiium field metaproteomes - sample provenance] - Trichodesmium sample provenance from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 2018 (Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-158.0
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Name		String
attribute	Name	bcodmo_name	String	sample
attribute	Name	description	String	Sample name
attribute	Name	long_name	String	Name
attribute	Name	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Name	units	String	unitless
variable	Replication		String
attribute	Replication	bcodmo_name	String	sample_descrip
attribute	Replication	description	String	Replication (singlicate|duplicate|triplicate)
attribute	Replication	long_name	String	Replication
attribute	Replication	units	String	unitless
variable	Cruise		String
attribute	Cruise	bcodmo_name	String	cruise_id
attribute	Cruise	description	String	Cruise identifier (or station BATS or HOT)
attribute	Cruise	long_name	String	Cruise
attribute	Cruise	units	String	unitless
variable	Cruise_station_num		byte
attribute	Cruise_station_num	_FillValue	byte	127
attribute	Cruise_station_num	actual_range	byte	1, 19
attribute	Cruise_station_num	bcodmo_name	String	station
attribute	Cruise_station_num	description	String	Station number
attribute	Cruise_station_num	long_name	String	Cruise Station Num
attribute	Cruise_station_num	units	String	unitless
variable	Time_sampled		String
attribute	Time_sampled	bcodmo_name	String	sample_descrip
attribute	Time_sampled	description	String	Description of time sampled
attribute	Time_sampled	long_name	String	Time Sampled
attribute	Time_sampled	units	String	unitless
variable	Date_sampled		String
attribute	Date_sampled	bcodmo_name	String	date
attribute	Date_sampled	description	String	Date of sample
attribute	Date_sampled	long_name	String	Date Sampled
attribute	Date_sampled	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date_sampled	source_name	String	Date_sampled
attribute	Date_sampled	time_precision	String	1970-01-01
attribute	Date_sampled	units	String	unitless
variable	Net_size		short
attribute	Net_size	_FillValue	short	32767
attribute	Net_size	actual_range	short	130, 200
attribute	Net_size	bcodmo_name	String	net_mesh
attribute	Net_size	description	String	Plankton net mesh size
attribute	Net_size	long_name	String	Net Size
attribute	Net_size	units	String	microns (um)
variable	Filter_size		float
attribute	Filter_size	_FillValue	float	NaN
attribute	Filter_size	actual_range	float	0.2, 5.0
attribute	Filter_size	bcodmo_name	String	filter_size
attribute	Filter_size	description	String	Supor filter size
attribute	Filter_size	long_name	String	Filter Size
attribute	Filter_size	units	String	microns (um)
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	0.17976, 31.4
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-158.0, -21.59
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	time_zone		String
attribute	time_zone	bcodmo_name	String	timezone
attribute	time_zone	description	String	Local time zone used for columns Date_sampled and Time_sampled.
attribute	time_zone	long_name	String	Time Zone
attribute	time_zone	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.