ERDDAP > info > bcodmo_dataset_806510

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Pacific Nitrite Oxidoreductase] - Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016 (Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients)		F   I   M	background			BCO-DMO	bcodmo_dataset_806510

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute	NC_GLOBAL	acquisition_description	String	Metaproteomics samples were collected by McLane pump onto 0.2 micron Supor membrane filters, with 51 and 3.0 micron prefilters. Global metaproteomic analyses were conducted using 1-dimensional (1D) and 2-dimensional (2D) chromatographic separation for the Metzyme and ProteOMZ expeditions respectively. Following global metaproteomic analyses, targeted metaproteomic assays were designed and samples were analyzed again by parallel reaction monitoring (PRM) mass spectrometry using mass spectral information from the global proteomic analyses. See methods in Saito et al., 2020 Nature Geosciences for full details. Samples were analyzed on a Thermo Fusion Orbitrap mass spectrometer. See methods in Saito et al., 2020 Nature Geosciences for full details. Data quality flags are included following GEOTRACES conventions for results below detection limit (flag= 6).\u00a0 Parameters were named using the prior GEOTRACES IDP parameter naming convention used for peptides (PEP), although these are new parameters not previously submitted here or elsewhere. The peptide\u00a0parameter naming convention was developed in collaboration with the GEOTRACES program. In other to avoid the sustainability challenge of having to maintain a parameter key of codes that represent protein and peptide sequences, the tryptic peptide amino acid sequences are inserted into the parameter name, with prefixes and suffixes for additional metadata. For example, the parameter name \"PEP_MTIQWGK_NxrA_PUMP\" has the following components. The prefix PEP refers to the peptides datatype, \"MTIQWGK\" refers to the specific amino acid sequence of the measured peptide, using the IUPAC- IUB 1 letter amino acid naming convention. This sequence can be used to calculate the molecular weight and elemental formula of the molecule that was measured (e.g. see [https://web.expasy.org/compute_pi/](\\"https://web.expasy.org/compute_pi/\\") and [https://web.expasy.org/protparam/](\\"https://web.expasy.org/protparam/\\")). \"NxrA\" refers to the protein name, in this case nitrite oxidoreductase subunit A. \"PUMP\" refers to the samping methodology, in order to differentiate when samples may be collected from the same location and depth but by different methods. This approach is useful as tryptic peptides are short enough in sequence to allow their use within parameter names, and each parameter name uniquely describes the molecule being measured, even when, as in this Nxr study, many different peptides are being measured from within a single protein. Reference:\u00a0[https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1984.tb...](\\"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1984.tb07877.x\\")
attribute	NC_GLOBAL	awards_0_award_nid	String	55017
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1031271
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1031271
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Donald L. Rice
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51467
attribute	NC_GLOBAL	awards_1_award_nid	String	646122
attribute	NC_GLOBAL	awards_1_award_number	String	GBMF3782
attribute	NC_GLOBAL	awards_1_data_url	String	https://www.moore.org/grant-detail?grantId=GBMF3782
attribute	NC_GLOBAL	awards_1_funder_name	String	Gordon and Betty Moore Foundation: Marine Microbiology Initiative
attribute	NC_GLOBAL	awards_1_funding_acronym	String	MMI
attribute	NC_GLOBAL	awards_1_funding_source_nid	String	385
attribute	NC_GLOBAL	awards_2_award_nid	String	724457
attribute	NC_GLOBAL	awards_2_award_number	String	OCE-1657766
attribute	NC_GLOBAL	awards_2_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1657766
attribute	NC_GLOBAL	awards_2_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_2_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_2_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_2_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_2_program_manager_nid	String	50534
attribute	NC_GLOBAL	awards_3_award_nid	String	785825
attribute	NC_GLOBAL	awards_3_award_number	String	OCE-1736599
attribute	NC_GLOBAL	awards_3_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736599
attribute	NC_GLOBAL	awards_3_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_3_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_3_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_3_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_3_program_manager_nid	String	51517
attribute	NC_GLOBAL	awards_4_award_nid	String	786678
attribute	NC_GLOBAL	awards_4_award_number	String	OCE-1850719
attribute	NC_GLOBAL	awards_4_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1850719
attribute	NC_GLOBAL	awards_4_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_4_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_4_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_4_program_manager	String	Daniel Thornhill
attribute	NC_GLOBAL	awards_4_program_manager_nid	String	722161
attribute	NC_GLOBAL	awards_5_award_nid	String	806568
attribute	NC_GLOBAL	awards_5_award_number	String	OCE-1924554
attribute	NC_GLOBAL	awards_5_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1924554
attribute	NC_GLOBAL	awards_5_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_5_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_5_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_5_program_manager	String	Dr Simone Metz
attribute	NC_GLOBAL	awards_5_program_manager_nid	String	51479
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Pacific Nitrite Oxidoreductase PI: Mak A. Saito Data Version 1: 2020-04-21
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2020-03-19T15:37:18Z
attribute	NC_GLOBAL	date_modified	String	2020-04-27T20:02:55Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.806510.1
attribute	NC_GLOBAL	Easternmost_Easting	double	156.0
attribute	NC_GLOBAL	geospatial_lat_max	double	17.0
attribute	NC_GLOBAL	geospatial_lat_min	double	-3.5
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	156.0
attribute	NC_GLOBAL	geospatial_lon_min	double	-160.77
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	1250.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	20.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/806510
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	McLane Pump
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	807118
attribute	NC_GLOBAL	instruments_0_description	String	McLane pumps sample large volumes of seawater at depth. They are attached to a wire and lowered to different depths in the ocean. As the water is pumped through the filter, particles suspended in the ocean are collected on the filters. The pumps are then retrieved and the contents of the filters are analyzed in a lab.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	McLane Pump
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	627
attribute	NC_GLOBAL	instruments_1_acronym	String	Mass Spec
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	806511
attribute	NC_GLOBAL	instruments_1_description	String	General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB16/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Mass Spectrometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	685
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Thermo Fusion Orbitrap mass spectrometer
attribute	NC_GLOBAL	keywords	String	aihgvyegvtifeapak, alivntpr, bco, bco-dmo, biological, cast, chemical, data, dataset, date, depth, dllgilqlfr, dmo, erddap, expedition, Fe_as_NxrAB, flag, fpnfgedtahgr, ggtlvavapeynppatk, glwepvr, icnhctypgclaacpr, iglnqqavgyvptdeewr, iso, lane, lanqvalldsiir, latitude, lhpddfipgyk, local, Long180, longitude, management, McLane_cast, McLane_ISO_DateTime_UTC, mean, mtiqwgk, nxr, NxrA_mean, NxrA_std, NxrAB_mean, NxrB_mean, NxrB_std, oceanography, office, pep, PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP, PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG, PEP_ALIVNTPR_NxrA_PUMP, PEP_ALIVNTPR_NxrA_PUMP_FLAG, PEP_DLLGILQLFR_NxrB_PUMP, PEP_DLLGILQLFR_NxrB_PUMP_FLAG, PEP_FPNFGEDTAHGR_NxrB_PUMP, PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG, PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP, PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG, PEP_GLWEPVR_NxrA_PUMP, PEP_GLWEPVR_NxrA_PUMP_FLAG, PEP_ICNHCTYPGCLAACPR_NxrB_PUMP, PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG, PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP, PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG, PEP_LANQVALLDSIIR_NxrA_PUMP, PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG, PEP_LHPDDFIPGYK_NxrA_PUMP, PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG, PEP_MTIQWGK_NxrA_PUMP, PEP_MTIQWGK_NxrA_PUMP_FLAG, PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP, PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG, preliminary, profiler, pump, salinity, salinity-temperature-depth, station, std, temperature, time, tqfyndepeaieygenfivhr
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/806510/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/806510
attribute	NC_GLOBAL	Northernmost_Northing	double	17.0
attribute	NC_GLOBAL	param_mapping	String	{'806510': {'Lat': 'flag - latitude', 'Long180': 'flag - longitude', 'Depth': 'master - depth', 'McLane_ISO_DateTime_UTC': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/806510/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Mak A. Saito
attribute	NC_GLOBAL	people_0_person_nid	String	50985
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Amber D. York
attribute	NC_GLOBAL	people_1_person_nid	String	643627
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	MetZyme,ProteOMZ (Proteomics in an Oxygen Minimum Zone),TriCoLim,PMT Cobalt and Metalloenzymes,MM Saito,Cyanobacteria Warming Responses,O2 Min Metalloenzyme
attribute	NC_GLOBAL	projects_0_acronym	String	MetZyme
attribute	NC_GLOBAL	projects_0_description	String	MetZyme project researchers will determine the role of enzymatic activity in the cycling of trace metals. Specifically the research will address the following questions: (1) degradation of sinking particulate organic material in the Tropical North Pacific can be influenced by the ability of microbes to synthesize zinc proteases, which in turn is controlled by the abundance or availability of zinc, and (2) methylation of mercury is controlled, in part, by the activity of cobalt-containing enzymes, and therefore the supply of labile cobalt to the corrinoid-containing enzymes or co-factors responsible for methylation. To attain their goal, they will collect dissolved and particulate samples for trace metals and metalloenzymes from three stations along a biogeochemical gradient in the Tropical North Pacific (along 150 degrees West from 18 degrees North to the equator). Sinking particles from metal clean sediment traps will also be obtained. The samples will also be used to carry out shipboard incubation experiments using amendments of metals, metal-chelators, B12, and proteases to examine the sensitivity and metal limitation of heterotrophic, enzymatic degradation of organic matter within the oceanic "Twilight Zone" (100-500 m). This study will result in a novel metaproteomic/metalloenzyme datasets that should provide insights into the biogeochemical cycling of metals, as well as co-limitation of primary productivity and controls on the export of carbon from the photic zone. In addition to the final data being contributed to BCO-DMO, an online metaproteomic data server will be created so the community has access to the raw data files generated by this research.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Tropical North Pacific along 150 degrees West from 18 degrees North to the equator
attribute	NC_GLOBAL	projects_0_name	String	Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients
attribute	NC_GLOBAL	projects_0_project_nid	String	2236
attribute	NC_GLOBAL	projects_0_start_date	String	2010-09
attribute	NC_GLOBAL	projects_1_acronym	String	ProteOMZ (Proteomics in an Oxygen Minimum Zone)
attribute	NC_GLOBAL	projects_1_description	String	From Schmidt Ocean Institute's ProteOMZ Project page: Rising temperatures, ocean acidification, and overfishing have now gained widespread notoriety as human-caused phenomena that are changing our seas. In recent years, scientists have increasingly recognized that there is yet another ingredient in that deleterious mix: a process called deoxygenation that results in less oxygen available in our seas. Large-scale ocean circulation naturally results in low-oxygen areas of the ocean called oxygen deficient zones (ODZs). The cycling of carbon and nutrients – the foundation of marine life, called biogeochemistry – is fundamentally different in ODZs than in oxygen-rich areas. Because researchers think deoxygenation will greatly expand the total area of ODZs over the next 100 years, studying how these areas function now is important in predicting and understanding the oceans of the future. This first expedition of 2016 led by Dr. Mak Saito from the Woods Hole Oceanographic Institution (WHOI) along with scientists from University of Maryland Center for Environmental Science, University of California Santa Cruz, and University of Washington aimed to do just that, investigate ODZs. During the 28 day voyage named “ProteOMZ,” researchers aboard R/V Falkor traveled from Honolulu, Hawaii to Tahiti to describe the biogeochemical processes that occur within this particular swath of the ocean’s ODZs. By doing so, they contributed to our greater understanding of ODZs, gathered a database of baseline measurements to which future measurements can be compared, and established a new methodology that could be used in future research on these expanding ODZs.
attribute	NC_GLOBAL	projects_1_geolocation	String	Central Pacific Ocean (Hawaii to Tahiti)
attribute	NC_GLOBAL	projects_1_name	String	The ProteOMZ Expedition: Investigating Life Without Oxygen in the Pacific Ocean
attribute	NC_GLOBAL	projects_1_project_nid	String	685696
attribute	NC_GLOBAL	projects_1_project_website	String	https://schmidtocean.org/cruise/investigating-life-without-oxygen-in-the-tropical-pacific/#team
attribute	NC_GLOBAL	projects_2_acronym	String	TriCoLim
attribute	NC_GLOBAL	projects_2_description	String	NSF abstract: Marine cyanobacteria are able to use or "fix" atmospheric nitrogen gas, and so supply much of the essential nutrient nitrogen that supports open ocean food chains. Oceanographers have usually thought that the growth of these nitrogen-fixing cyanobacteria is limited at any particular time and place by the supply of either iron, or of phosphorus. Preliminary experiments have shown, though, that these nitrogen fixers instead grow best when both iron and phosphorus are scarce at the same time. In this project, the researchers will use cellular indicators that are specific for iron and phosphorus limitation to determine how important this type of "balanced limitation" of nitrogen-fixing cyanobacteria is in controlling the productivity of ocean food chains in the tropical Atlantic Ocean. Two graduate students will be trained at the University of Southern California (USC) and Woods Hole Oceanographic Institution, as well as a postdoctoral researcher at USC. Educational outreach efforts will take place at a Los Angeles inner city high school with a student body that is over 98% Hispanic and African-American, and with underrepresented undergraduates in the USC Global Environmental Microbiology course. In addition, two Research Experiences for Undergraduates students will be supervised for summer research projects to help them learn about science career options. The researchers will investigate the biological and biogeochemical consequences of this unique balanced iron/phosphorus-limited phenotype, using both laboratory and fieldwork approaches. During the first year of this project, the nitrogen-fixing cyanobacteria will be cultured under iron and/or phosphorus limitation, followed by application of proteomics and transcriptomics to identify genes that are potential diagnostic biomarkers for iron/phosphorus balanced limitation. Preliminary work has already identified one promising candidate biomarker in one cyanobacterium, an EzrA protein domain that appears to be associated with the cell size decreases seen specifically under balanced limitation, and the researchers have identified numerous other potential candidates for similar biomarkers. During the second year, these new co-limitation biomarkers and others previously validated for iron limitation (IsiB) and phosphorus limitation (SphX) will be used to investigate balanced limitation during a research cruise transecting from relatively high-iron, low-phosphorus North Atlantic waters, to the relatively high-phosphorus, low-iron South Atlantic. This fieldwork component will survey nitrogen fixing cyanobacteria populations across this natural iron/phosphorus gradient for genetic, proteomic, and physiological indicators of balanced limitation, as well as testing their responses to iron and phosphorus manipulations in shipboard incubation experiments. The third year will be devoted to sample analysis, and publications exploring the responses of oceanic nitrogen fixers to simultaneous limitation by both iron and phosphorus.
attribute	NC_GLOBAL	projects_2_end_date	String	2020-02
attribute	NC_GLOBAL	projects_2_geolocation	String	Tropical Atlantic
attribute	NC_GLOBAL	projects_2_name	String	Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
attribute	NC_GLOBAL	projects_2_project_nid	String	724451
attribute	NC_GLOBAL	projects_2_start_date	String	2017-03
attribute	NC_GLOBAL	projects_3_acronym	String	PMT Cobalt and Metalloenzymes
attribute	NC_GLOBAL	projects_3_description	String	NSF abstract: Cobalt is important for many forms of marine life, yet it is one of the scarcest nutrients in the sea. Cobalt's oceanic abundance and distribution, along with other scarce nutrients, can influence the growth of microscopic plants (phytoplankton). This in turn can influence carbon cycles in the ocean and atmosphere. Therefore, knowledge of the controls on cobalt's abundance and chemical forms in seawater is a valuable component of our ability to understand the ocean's influence on global carbon cycling. Within phytoplankton and other marine microbes, metals such as cobalt, iron, nickel, and copper are used as critical components of enzymes responsible for key cellular reactions. Since these enzymes require metals to work, they are named metalloenzymes. Participating in a Pacific Ocean cruise from Alaska to Tahiti, this project will study the oceanic distributions of dissolved cobalt and the cellular content of a group of metalloenzymes known to influence biogeochemical cycles. The project will provide scientific impact by creating new knowledge about oceanic micronutrients in regions of economic interest with regard to fisheries and deep-sea mining. Measurement of proteins in the North Pacific will provide data of broad biological and chemical interest and will be made available through a new NSF-funded "EarthCube Ocean Protein Portal" data base. Educational impact will stem from participation of a graduate student and two young technicians, as well as the PI's development of a high school chemistry curriculum for use in two local high schools, thus allowing teachers to include real oceanic and environmental data at their first introduction to chemistry. Cobalt has a complex biogeochemical cycle. Both its inorganic and organic forms are used by biology in the upper ocean and it is removed from solution by being scavenged in the intermediate and deep ocean. This scavenging removal results in cobalt having the smallest oceanic inventory of any biologically utilized element. Recent studies, however, have found that large dissolved cobalt plumes occur in major oxygen minimum zones due to a combination of less scavenging and additions from sedimentary and remineralization fluxes. The GP15 US GEOTRACES Pacific Meridional Transect (PMT) provides an opportunity to examine the influence of oxygen depletion on cobalt chemistry. Moreover, the study of the protein component of microbial communities using new proteomic techniques will provide evidence of how different major microorganisms respond to the chemical environment (e.g. through transporter production for specific nutrients and micronutrients) as well as the biochemical basis for metal requirements related to the use of specific metalloenzymes. Specifically, the PMT provides an opportunity to confirm that the Pacific oxygen minimum zones contain a large amount of cobalt and to test the hypotheses that simultaneous zinc scarcity could induce wide-scale biochemical substitution of cobalt for zinc in the North Pacific Ocean.
attribute	NC_GLOBAL	projects_3_end_date	String	2019-10
attribute	NC_GLOBAL	projects_3_geolocation	String	Laboratory Study and Cultures from Northeast Pacific Line P Transect 48.8167 N 128.667 W
attribute	NC_GLOBAL	projects_3_name	String	US GEOTRACES PMT: Cobalt Biogeochemical Cycling and Connections to Metalloenzymes in the Pacific Ocean
attribute	NC_GLOBAL	projects_3_project_nid	String	785826
attribute	NC_GLOBAL	projects_3_start_date	String	2017-11
attribute	NC_GLOBAL	projects_4_acronym	String	MM Saito
attribute	NC_GLOBAL	projects_4_description	String	In support of obtaining deeper knowledge of major biogeochemically relevant proteins to inform a mechanistic understanding of global marine biogeochemical cycles.
attribute	NC_GLOBAL	projects_4_end_date	String	2019-12
attribute	NC_GLOBAL	projects_4_name	String	Marine Microbial Investigator Award: Investigator Mak Saito
attribute	NC_GLOBAL	projects_4_project_nid	String	786672
attribute	NC_GLOBAL	projects_4_start_date	String	2013-05
attribute	NC_GLOBAL	projects_5_acronym	String	Cyanobacteria Warming Responses
attribute	NC_GLOBAL	projects_5_description	String	NSF abstract: The oceans absorb much of the heat generated by human activities, and this warming of the surface ocean has consequences for important groups of marine organisms. Marine cyanobacteria are one such key group of organisms, since they supply much of the essential carbon and nitrogen that supports nearly all the rest of the marine food web. Currently, the growth of cyanobacteria is mostly constrained by scarce supplies of the micronutrient element iron, but they are also very sensitive to the ongoing increases in seawater temperature. Preliminary results suggest that warming could partly mitigate the negative effects of iron limitation on marine cyanobacteria. This project examines in depth how these interactions between warming and iron limitation will affect the future ocean carbon and nitrogen cycles, using laboratory culture experiments showing how cyanobacteria respond to simultaneously changing temperature and iron supplies. Both short-term response studies and long-term evolutionary experiments testing for adaptation use a comprehensive set of molecular biology tools targeting genes to proteins. The final goal is to apply the results of these experiments to improve quantitative models predicting how the ocean's carbon and nitrogen cycles, biological productivity, and living resources will respond to a warming future climate. Two graduate students, a postdoc and 3-4 underrepresented undergraduate researchers are supported, and the investigators also mentor summer science interns from largely Hispanic local high schools. The physiology, biochemistry and biogeography of nitrogen-fixing cyanobacteria and unicellular picocyanobacteria are strongly influenced by temperature, subjecting them to intense selective pressure as the modern ocean steadily warms up. These groups have likewise been rigorously selected under chronic iron (Fe) scarcity, and the availability of this crucial micronutrient is also changing with a shifting climate. This project examines short-term acclimation and long-term evolutionary responses of Fe-stressed marine cyanobacteria to a warmer environment. Preliminary data show that Iron Use Efficiencies (IUE, mols N fixed.hr-1 mol cellular Fe-1) of Fe-limited Trichodesmium increase 4 to 5-fold with a 5oC temperature increase, allowing the cells to much more efficiently leverage scarce available Fe supplies to grow and fix nitrogen. This means that warming can to a large degree mitigate the negative effects of Fe limitation on Trichodesmium, resulting in a modelled 22% increase in global nitrogen fixation by 2100 in a warmer climate. This project aims to uncover the cellular biochemical mechanisms involved in this Fe-limitation/thermal IUE effect in a four-year experimental evolution study of the diazotrophs Trichodesmium and Crocosphaera and the picocyanobacteria Synechococcus and Prochlorococcus, under a multi-variate selection matrix of temperature and Fe availability. The objectives are to 1) Assess the long-term adaptive responses of fitness, IUE and physiology to Fe limitation and warming interactions in these four major cyanobacterial groups; 2) Determine the molecular and biochemical mechanisms behind the surprising Fe/warming interactive effect on IUE using genomics, transcriptomics and quantitative proteomics coupled with 'metalloproteomics' determinations of Fe content in critical proteins; 3) Compare and contrast acclimation and adaptation responses to Fe limitation and warming in key cyanobacteria taxa, and 4) Integrate results using a published biogeochemical modeling approach to assess global consequences for marine productivity and nitrogen fixation. This project offers a mechanistic and predictive understanding of adaptation to Fe and warming co-stressors in a rapidly changing future ocean environment for some of the most important photoautotrophic functional groups in the ocean. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
attribute	NC_GLOBAL	projects_5_end_date	String	2023-03
attribute	NC_GLOBAL	projects_5_name	String	Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions
attribute	NC_GLOBAL	projects_5_project_nid	String	786679
attribute	NC_GLOBAL	projects_5_start_date	String	2019-04
attribute	NC_GLOBAL	projects_6_acronym	String	O2 Min Metalloenzyme
attribute	NC_GLOBAL	projects_6_description	String	NSF abstract: Though scarce and largely insoluble, trace metals are key components of sophisticated enzymes (protein molecules that speed up biochemical reactions) involved in biogeochemical cycles in the dark ocean (below 1000m). For example, metalloenzymes are involved in nearly every reaction in the nitrogen cycle. Yet, despite direct connections between trace metal and nitrogen cycles, the relationship between trace metal distributions and biological nitrogen cycling processes in the dark ocean have rarely been explored, likely due to the technical challenges associated with their study. Availability of the autonomous underwater vehicle (AUV) Clio, a sampling platform capable of collecting high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material, has overcome this challenge. Thus, this research project plans an interdisciplinary chemistry, biology, and engineering effort to test the hypothesis that certain chemical reactions, such as nitrite oxidation, could become limited by metal availability within the upper mesopelagic and that trace metal demands for nitrite-oxidizing bacteria may be increased under low oxygen conditions. Broader impacts of this study include the continued development and application of the Clio Biogeochemical AUV as a community resource by developing and testing its high-resolution and adaptive sampling capabilities. In addition, metaproteomic data will be deposited into the recently launched Ocean Protein Portal to allow oceanographers and the metals in biology community to examine the distribution of proteins and metalloenzymes in the ocean. Undergraduate students will be supported by this project at all three institutions, with an effort to recruit minority students. The proposed research will also be synergistic with the goals of early community-building efforts for a potential global scale microbial biogeochemistry program modeled after the success of the GEOTRACES program, provisionally called "Biogeoscapes: Ocean metabolism and nutrient cycles on a changing planet". The proposed research project will test the following three hypotheses: (1) the microbial metalloenzyme distribution of the mesopelagic is spatially dynamic in response to environmental gradients in oxygen and trace metals, (2) nitrite oxidation in the Eastern Tropical Pacific Ocean can be limited by iron availability in the upper mesopelagic through an inability to complete biosynthesis of the microbial protein nitrite oxidoreductase, and (3) nitrite-oxidizing bacteria increase their metalloenzyme requirements at low oxygen, impacting the distribution of both dissolved and particulate metals within oxygen minimum zones. One of the challenges to characterizing the biogeochemistry of the mesopelagic ocean is an inability to effectively sample it. As a sampling platform, we will use the novel biogeochemical AUV Clio that enables high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material on a research expedition in the Eastern Tropical Pacific Ocean. Specific research activities will be orchestrated to test the hypotheses. Hypothesis 1 will be explored by comparison of hydrographic, microbial distributions, dissolved and particulate metal data, and metaproteomic results with profile samples collected by Clio. Hypothesis 2 will be tested by incubation experiments using 15NO2- oxidation rates on Clio-collected incubation samples. Hypothesis 3 will be tested by dividing targeted nitrite oxidoreductase protein copies by qPCR (quantitative polymerase chain reaction)-based nitrite oxidizing bacteria abundance (NOB) to determine if cellular copy number varies with oxygen distributions, and by metalloproteomic analyses of NOB cultures. The demonstration of trace metal limitation of remineralization processes, not just primary production, would transform our understanding of the role of metals in biogeochemical cycling and provide new ways with which to interpret sectional data of dissolved and particulate trace metal distributions in the ocean. The idea that oxygen may play a previously underappreciated role in controlling trace metals due not just to metals' physical chemistry, but also from changing biological demand, will improve our ability to predict trace metal distributions in the face of decreasing ocean oxygen content. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
attribute	NC_GLOBAL	projects_6_end_date	String	2022-10
attribute	NC_GLOBAL	projects_6_geolocation	String	Eastern Tropical Pacific
attribute	NC_GLOBAL	projects_6_name	String	Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories
attribute	NC_GLOBAL	projects_6_project_nid	String	806565
attribute	NC_GLOBAL	projects_6_start_date	String	2019-11
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	-3.5
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016. NxrA and NxrB peptide concentrations in fmol/L. Peptide names are using the GEOTRACES naming convention (PEP for peptide, full tryptic peptide amino acid sequence, Protein name, Sampling device (=Pump)). Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit. These data were published in Saito et al., 2020 as Supplementary Table 1.
attribute	NC_GLOBAL	time_coverage_end	String	2016-01-27T06:30Z
attribute	NC_GLOBAL	time_coverage_start	String	2011-10-05T20:00Z
attribute	NC_GLOBAL	title	String	[Pacific Nitrite Oxidoreductase] - Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016 (Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-160.77
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	Expedition		String
attribute	Expedition	bcodmo_name	String	cruise_id
attribute	Expedition	description	String	Expedition (Cruise) identifier
attribute	Expedition	long_name	String	Expedition
attribute	Expedition	units	String	unitless
variable	Station		byte
attribute	Station	_FillValue	byte	127
attribute	Station	actual_range	byte	1, 8
attribute	Station	bcodmo_name	String	station
attribute	Station	description	String	Station
attribute	Station	long_name	String	Station
attribute	Station	units	String	unitless
variable	Long		float
attribute	Long	_FillValue	float	NaN
attribute	Long	actual_range	float	140.0, 205.6
attribute	Long	bcodmo_name	String	lon_360
attribute	Long	colorBarMaximum	double	180.0
attribute	Long	colorBarMinimum	double	-180.0
attribute	Long	description	String	Longitude (0 to 360 degrees)
attribute	Long	long_name	String	Longitude
attribute	Long	source_name	String	Long
attribute	Long	standard_name	String	longitude
attribute	Long	units	String	decimal degrees
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-160.77, 156.0
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude (-180 to 180)
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	-3.5, 17.0
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Lattitude
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	McLane_cast		String
attribute	McLane_cast	bcodmo_name	String	cast
attribute	McLane_cast	description	String	McLane pump cast identifier
attribute	McLane_cast	long_name	String	Mc Lane Cast
attribute	McLane_cast	units	String	unitless
variable	McLane_time_local		String
attribute	McLane_time_local	bcodmo_name	String	DateTime
attribute	McLane_time_local	description	String	McLane pump date and time (local time zone HST, UTC-10) in format yyyy-mm-ddTHH:MM
attribute	McLane_time_local	long_name	String	Mc Lane Time Local
attribute	McLane_time_local	source_name	String	McLane_time_local
attribute	McLane_time_local	time_precision	String	1970-01-01T00:00Z
attribute	McLane_time_local	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.3178448E9, 1.4538762E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	McLane pump date and time (time zone UTC) in ISO 8601 format yyyy-mm-ddTHH:MMZ
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	Mc Lane ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	20.0, 1250.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	Sample depth
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	PEP_LANQVALLDSIIR_NxrA_PUMP		float
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	actual_range	float	0.0, 73.17
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [LANQVALLDSIIR] from protein NxrA from McLane pump samples.
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	long_name	String	PEP LANQVALLDSIIR Nxr A PUMP
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG		byte
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_LANQVALLDSIIR_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	long_name	String	PEP LANQVALLDSIIR Nxr A PUMP FLAG
attribute	PEP_LANQVALLDSIIR_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP		float
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	actual_range	float	0.0, 288.52
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [GGTLVAVAPEYNPPATK] from protein NxrA from McLane pump samples.
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	long_name	String	PEP GGTLVAVAPEYNPPATK Nxr A PUMP
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG		byte
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	long_name	String	PEP GGTLVAVAPEYNPPATK Nxr A PUMP FLAG
attribute	PEP_GGTLVAVAPEYNPPATK_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_MTIQWGK_NxrA_PUMP		float
attribute	PEP_MTIQWGK_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_MTIQWGK_NxrA_PUMP	actual_range	float	0.03, 108.11
attribute	PEP_MTIQWGK_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_MTIQWGK_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [MTIQWGK] from protein NxrA from McLane pump samples.
attribute	PEP_MTIQWGK_NxrA_PUMP	long_name	String	PEP MTIQWGK Nxr A PUMP
attribute	PEP_MTIQWGK_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_MTIQWGK_NxrA_PUMP_FLAG		byte
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_MTIQWGK_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	long_name	String	PEP MTIQWGK Nxr A PUMP FLAG
attribute	PEP_MTIQWGK_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_LHPDDFIPGYK_NxrA_PUMP		float
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	actual_range	float	0.06, 227.77
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [LHPDDFIPGYK] from protein NxrA from McLane pump samples.
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	long_name	String	PEP LHPDDFIPGYK Nxr A PUMP
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG		byte
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_LHPDDFIPGYK_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	long_name	String	PEP LHPDDFIPGYK Nxr A PUMP FLAG
attribute	PEP_LHPDDFIPGYK_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_ALIVNTPR_NxrA_PUMP		float
attribute	PEP_ALIVNTPR_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_ALIVNTPR_NxrA_PUMP	actual_range	float	0.0, 391.34
attribute	PEP_ALIVNTPR_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_ALIVNTPR_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [ALIVNTPR] from protein NxrA from McLane pump samples.
attribute	PEP_ALIVNTPR_NxrA_PUMP	long_name	String	PEP ALIVNTPR Nxr A PUMP
attribute	PEP_ALIVNTPR_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_ALIVNTPR_NxrA_PUMP_FLAG		byte
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_ALIVNTPR_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	long_name	String	PEP ALIVNTPR Nxr A PUMP FLAG
attribute	PEP_ALIVNTPR_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP		float
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	actual_range	float	0.0, 85.94
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [TQFYNDEPEAIEYGENFIVHR] from protein NxrA from McLane pump samples.
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	long_name	String	PEP TQFYNDEPEAIEYGENFIVHR Nxr A PUMP
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG		byte
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	long_name	String	PEP TQFYNDEPEAIEYGENFIVHR Nxr A PUMP FLAG
attribute	PEP_TQFYNDEPEAIEYGENFIVHR_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_GLWEPVR_NxrA_PUMP		float
attribute	PEP_GLWEPVR_NxrA_PUMP	_FillValue	float	NaN
attribute	PEP_GLWEPVR_NxrA_PUMP	actual_range	float	0.0, 174.07
attribute	PEP_GLWEPVR_NxrA_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_GLWEPVR_NxrA_PUMP	description	String	Peptide amino acid sequence concentration [GLWEPVR] from protein NxrA from McLane pump samples.
attribute	PEP_GLWEPVR_NxrA_PUMP	long_name	String	PEP GLWEPVR Nxr A PUMP
attribute	PEP_GLWEPVR_NxrA_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_GLWEPVR_NxrA_PUMP_FLAG		byte
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	description	String	Quality flag for column PEP_GLWEPVR_NxrA_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	long_name	String	PEP GLWEPVR Nxr A PUMP FLAG
attribute	PEP_GLWEPVR_NxrA_PUMP_FLAG	units	String	unitless
variable	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP		float
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	_FillValue	float	NaN
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	actual_range	float	0.0, 119.69
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	description	String	Peptide amino acid sequence concentration [AIHGVYEGVTIFEAPAK] from protein NxrB from McLane pump samples.
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	long_name	String	PEP AIHGVYEGVTIFEAPAK Nxr B PUMP
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG		byte
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	description	String	Quality flag for column PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	long_name	String	PEP AIHGVYEGVTIFEAPAK Nxr B PUMP FLAG
attribute	PEP_AIHGVYEGVTIFEAPAK_NxrB_PUMP_FLAG	units	String	unitless
variable	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP		float
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	_FillValue	float	NaN
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	actual_range	float	0.0, 416.82
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	colorBarMaximum	double	150.0
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	colorBarMinimum	double	0.0
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	description	String	Peptide amino acid sequence concentration [IGLNQQAVGYVPTDEEWR] from protein NxrB from McLane pump samples.
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	long_name	String	PEP IGLNQQAVGYVPTDEEWR Nxr B PUMP
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG		byte
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	description	String	Quality flag for column PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	long_name	String	PEP IGLNQQAVGYVPTDEEWR Nxr B PUMP FLAG
attribute	PEP_IGLNQQAVGYVPTDEEWR_NxrB_PUMP_FLAG	units	String	unitless
variable	PEP_FPNFGEDTAHGR_NxrB_PUMP		float
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	_FillValue	float	NaN
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	actual_range	float	0.01, 135.91
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	description	String	Peptide amino acid sequence concentration [FPNFGEDTAHGR] from protein NxrB from McLane pump samples.
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	long_name	String	PEP FPNFGEDTAHGR Nxr B PUMP
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG		byte
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	description	String	Quality flag for column PEP_FPNFGEDTAHGR_NxrB_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	long_name	String	PEP FPNFGEDTAHGR Nxr B PUMP FLAG
attribute	PEP_FPNFGEDTAHGR_NxrB_PUMP_FLAG	units	String	unitless
variable	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP		float
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	_FillValue	float	NaN
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	actual_range	float	0.01, 238.41
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	description	String	Peptide amino acid sequence concentration [ICNHCTYPGCLAACPR] from protein NxrB from McLane pump samples.
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	long_name	String	PEP ICNHCTYPGCLAACPR Nxr B PUMP
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG		byte
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	description	String	Quality flag for column PEP_ICNHCTYPGCLAACPR_NxrB_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	long_name	String	PEP ICNHCTYPGCLAACPR Nxr B PUMP FLAG
attribute	PEP_ICNHCTYPGCLAACPR_NxrB_PUMP_FLAG	units	String	unitless
variable	PEP_DLLGILQLFR_NxrB_PUMP		float
attribute	PEP_DLLGILQLFR_NxrB_PUMP	_FillValue	float	NaN
attribute	PEP_DLLGILQLFR_NxrB_PUMP	actual_range	float	0.0, 90.07
attribute	PEP_DLLGILQLFR_NxrB_PUMP	bcodmo_name	String	amino_conc
attribute	PEP_DLLGILQLFR_NxrB_PUMP	description	String	Peptide amino acid sequence concentration [DLLGILQLFR] from protein NxrB from McLane pump samples.
attribute	PEP_DLLGILQLFR_NxrB_PUMP	long_name	String	PEP DLLGILQLFR Nxr B PUMP
attribute	PEP_DLLGILQLFR_NxrB_PUMP	units	String	femtomoles per liter (fmol/L)
variable	PEP_DLLGILQLFR_NxrB_PUMP_FLAG		byte
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	_FillValue	byte	127
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	actual_range	byte	1, 6
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	bcodmo_name	String	q_flag
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	colorBarMaximum	double	150.0
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	colorBarMinimum	double	0.0
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	description	String	Quality flag for column PEP_DLLGILQLFR_NxrB_PUMP. Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit.
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	long_name	String	PEP DLLGILQLFR Nxr B PUMP FLAG
attribute	PEP_DLLGILQLFR_NxrB_PUMP_FLAG	units	String	unitless
variable	NxrA_mean		float
attribute	NxrA_mean	_FillValue	float	NaN
attribute	NxrA_mean	actual_range	float	0.0162, 162.8059
attribute	NxrA_mean	bcodmo_name	String	mean
attribute	NxrA_mean	description	String	Average of NxrA peptides
attribute	NxrA_mean	long_name	String	Nxr A Mean
attribute	NxrA_mean	units	String	femtomoles per liter (fmol/L)
variable	NxrA_std		float
attribute	NxrA_std	_FillValue	float	NaN
attribute	NxrA_std	actual_range	float	0.0223, 137.441
attribute	NxrA_std	bcodmo_name	String	standard deviation
attribute	NxrA_std	description	String	Standard Deviation of NxrA peptides
attribute	NxrA_std	long_name	String	Nxr A Std
attribute	NxrA_std	units	String	femtomoles per liter (fmol/L)
variable	NxrB_mean		float
attribute	NxrB_mean	_FillValue	float	NaN
attribute	NxrB_mean	actual_range	float	0.0113, 155.0874
attribute	NxrB_mean	bcodmo_name	String	mean
attribute	NxrB_mean	description	String	Average of NxrB peptides
attribute	NxrB_mean	long_name	String	Nxr B Mean
attribute	NxrB_mean	units	String	femtomoles per liter (fmol/L)
variable	NxrB_std		float
attribute	NxrB_std	_FillValue	float	NaN
attribute	NxrB_std	actual_range	float	0.0045, 168.6216
attribute	NxrB_std	bcodmo_name	String	standard deviation
attribute	NxrB_std	description	String	Standard Deviation of NxrB peptides
attribute	NxrB_std	long_name	String	NXR B STD
attribute	NxrB_std	units	String	femtomoles per liter (fmol/L)
variable	NxrAB_mean		float
attribute	NxrAB_mean	_FillValue	float	NaN
attribute	NxrAB_mean	actual_range	float	0.0137, 145.8915
attribute	NxrAB_mean	bcodmo_name	String	mean
attribute	NxrAB_mean	description	String	Average of NxrA and NxrB averages
attribute	NxrAB_mean	long_name	String	Nxr AB Mean
attribute	NxrAB_mean	units	String	femtomoles per liter (fmol/L)
variable	Fe_as_NxrAB		double
attribute	Fe_as_NxrAB	_FillValue	double	NaN
attribute	Fe_as_NxrAB	actual_range	double	3.151E-4, 3.3555045
attribute	Fe_as_NxrAB	bcodmo_name	String	Fe
attribute	Fe_as_NxrAB	description	String	Concentration of Fe within NxrAB
attribute	Fe_as_NxrAB	long_name	String	Fe As Nxr AB
attribute	Fe_as_NxrAB	units	String	picomoles per liter (pmol/L)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.