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Title Sum-
Institution Dataset ID
     data   graph     files  public Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in
zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G.
Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Location: Cultures collected from Northeast Pacific Line P Transect 48.8167 N
128.667 W

Metal quotas
Cellular metal quotas were measured by inductively coupled plasma mass
spectrometry (ICP-MS). Biomass from replicate 25 mL matrix cultures of P.
tricornutum CCMP632, P. delicatissima UNC1205, and Thalassiosira UNC1203 were
pooled upon entering stationary phase and were centrifuged at 11,000 RPM
(14,610 x g) for 40 minutes at 4\u00b0C. The cell pellet was resuspended in ~1
mL media and transferred to an acid-cleaned microcentrifuge tube. Cultures
were centrifuged again for 30 min at 14,100 RPM (13,336 x g) at 4\u00b0C
before the supernatant was discarded. The remaining cell pellet was acidified
in 800 L of 5% nitric acid (Optima) containing 1 ppb indium for at least seven
days. Solids were removed by centrifugation. No attempt was made to remove
extracellular metals by washing cells with additional metal chelators in order
to minimize processing blanks. Quota determinations therefore include
contributions from both intracellular and extracellular pools. Process blank
digestions containing acid but no cells were performed in parallel. Digests
were diluted by a factor of 9 with 5% nitric acid 1 ppb indium solution before
being analyzed in duplicate on a Thermo ICAP-Q plasma mass spectrometer
calibrated to a multi-element standard curve (Spex Certiprep) over a range of
1 \u2013 20 ppb. Samples were analyzed in KED mode after an 85s sample uptake
window and element mass windows were scanned 3 times during measurements. The
1 ppb indium internal standard was used to correct for variation in sample
delivery and plasma suppression between samples. Process blanks were
subtracted from measured concentrations. Phosphorus concentrations were also
measured by ICP-MS simultaneously and were calibrated to a standard curve
ranging from 100 \u2013 3,200 ppb using a 1 ppm certified P stock (Alfa Aesar
Specpure). The seawater media base used for all growth experiments was
similarly analyzed via ICP-MS using a 1:10 dilution of media base into 5%
nitric acid 1 ppb indium and analyzed as above to determine background media
concentrations of total Zn and Co (0.9 nmol L-1 and 0.1 nmol L-1,

Missing data identifiers in this dataset include:
* "nd" indicating no data
* Below Detection
* "contam" indicating the sample was contaminated

Isolation sources and locations
* Pseudonitzschia delicatissima UNC1205 and Thalassiosira UNC1203 were
isolated from
station P8 of the Line P transect, 48.817\uf0b0N 128.666\uf0b0W
* Phaeodactylum tricornutum CCMP632 was ordered from Bigelow, the strains
location of isolation was 54\uf0b0N 4\uf0b0W
* Thalassiosira pseudonana CCMP1335 was also from Bigelow, original location
isolation was 40.756\u00b0 N 72.82\u00b0 W
attribute NC_GLOBAL awards_0_award_nid String 646122
attribute NC_GLOBAL awards_0_award_number String GBMF3782
attribute NC_GLOBAL awards_0_data_url String https://www.moore.org/grant-detail?grantId=GBMF3782 (external link)
attribute NC_GLOBAL awards_0_funder_name String Gordon and Betty Moore Foundation: Marine Microbiology Initiative
attribute NC_GLOBAL awards_0_funding_acronym String MMI
attribute NC_GLOBAL awards_0_funding_source_nid String 385
attribute NC_GLOBAL awards_1_award_nid String 785825
attribute NC_GLOBAL awards_1_award_number String OCE-1736599
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736599 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_1_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Line P Metal quotas
PI: Mak Saito
Data Version 1: 2020-03-31
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2020-03-31T20:11:49Z
attribute NC_GLOBAL date_modified String 2020-04-10T14:55:44Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.807299.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/807299 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 807325
attribute NC_GLOBAL instruments_0_description String General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 685
attribute NC_GLOBAL instruments_0_supplied_name String Thermo ICAP-Q plasma mass spectrometer
attribute NC_GLOBAL keywords String added, Added_Co, Added_Zn, average, bco, bco-dmo, biological, CD_to_P_avg, chemical, co2plus, CO_to_P_avg, CU_to_P_avg, culture, data, dataset, dmo, erddap, FE_to_P_avg, log, log_Co2plus, log_Zn2plus, management, MN_to_P_avg, MO_to_P_avg, NI_to_P_avg, oceanography, office, preliminary, total, Total_Co, Total_Zn, zn2plus, ZN_to_P_avg
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/807299/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/807299 (external link)
attribute NC_GLOBAL param_mapping String {'807299': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/807299/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_0_affiliation_acronym String WHOI
attribute NC_GLOBAL people_0_person_name String Mak A. Saito
attribute NC_GLOBAL people_0_person_nid String 50985
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Amber York
attribute NC_GLOBAL people_1_person_nid String 643627
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String PMT Cobalt and Metalloenzymes,MM Saito
attribute NC_GLOBAL projects_0_acronym String PMT Cobalt and Metalloenzymes
attribute NC_GLOBAL projects_0_description String NSF abstract:
Cobalt is important for many forms of marine life, yet it is one of the scarcest nutrients in the sea. Cobalt's oceanic abundance and distribution, along with other scarce nutrients, can influence the growth of microscopic plants (phytoplankton). This in turn can influence carbon cycles in the ocean and atmosphere. Therefore, knowledge of the controls on cobalt's abundance and chemical forms in seawater is a valuable component of our ability to understand the ocean's influence on global carbon cycling. Within phytoplankton and other marine microbes, metals such as cobalt, iron, nickel, and copper are used as critical components of enzymes responsible for key cellular reactions. Since these enzymes require metals to work, they are named metalloenzymes. Participating in a Pacific Ocean cruise from Alaska to Tahiti, this project will study the oceanic distributions of dissolved cobalt and the cellular content of a group of metalloenzymes known to influence biogeochemical cycles. The project will provide scientific impact by creating new knowledge about oceanic micronutrients in regions of economic interest with regard to fisheries and deep-sea mining. Measurement of proteins in the North Pacific will provide data of broad biological and chemical interest and will be made available through a new NSF-funded "EarthCube Ocean Protein Portal" data base. Educational impact will stem from participation of a graduate student and two young technicians, as well as the PI's development of a high school chemistry curriculum for use in two local high schools, thus allowing teachers to include real oceanic and environmental data at their first introduction to chemistry.
Cobalt has a complex biogeochemical cycle. Both its inorganic and organic forms are used by biology in the upper ocean and it is removed from solution by being scavenged in the intermediate and deep ocean. This scavenging removal results in cobalt having the smallest oceanic inventory of any biologically utilized element. Recent studies, however, have found that large dissolved cobalt plumes occur in major oxygen minimum zones due to a combination of less scavenging and additions from sedimentary and remineralization fluxes. The GP15 US GEOTRACES Pacific Meridional Transect (PMT) provides an opportunity to examine the influence of oxygen depletion on cobalt chemistry. Moreover, the study of the protein component of microbial communities using new proteomic techniques will provide evidence of how different major microorganisms respond to the chemical environment (e.g. through transporter production for specific nutrients and micronutrients) as well as the biochemical basis for metal requirements related to the use of specific metalloenzymes. Specifically, the PMT provides an opportunity to confirm that the Pacific oxygen minimum zones contain a large amount of cobalt and to test the hypotheses that simultaneous zinc scarcity could induce wide-scale biochemical substitution of cobalt for zinc in the North Pacific Ocean.
attribute NC_GLOBAL projects_0_end_date String 2019-10
attribute NC_GLOBAL projects_0_geolocation String Laboratory Study and Cultures from Northeast Pacific Line P Transect 48.8167 N 128.667 W
attribute NC_GLOBAL projects_0_name String US GEOTRACES PMT: Cobalt Biogeochemical Cycling and Connections to Metalloenzymes in the Pacific Ocean
attribute NC_GLOBAL projects_0_project_nid String 785826
attribute NC_GLOBAL projects_0_start_date String 2017-11
attribute NC_GLOBAL projects_1_acronym String MM Saito
attribute NC_GLOBAL projects_1_description String In support of obtaining deeper knowledge of major biogeochemically relevant proteins to inform a mechanistic understanding of global marine biogeochemical cycles.
attribute NC_GLOBAL projects_1_end_date String 2019-12
attribute NC_GLOBAL projects_1_name String Marine Microbial Investigator Award: Investigator Mak Saito
attribute NC_GLOBAL projects_1_project_nid String 786672
attribute NC_GLOBAL projects_1_start_date String 2013-05
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Metal quotas of two NE Pacific Line P diatom isolates and one Atlantic diatom isolate measured via ICP-MS after growth in zinc and cobalt media amendments. Experiments with cultures collected from the GeoMICS expedition on the R/V Thomas G. Thompson (cruise TN280), along Line P in the NE Pacific, in May of 2012.
attribute NC_GLOBAL title String Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Culture   String  
attribute Culture bcodmo_name String sample_descrip
attribute Culture description String Culture name (e.g. Thalassiosira UNC12032)
attribute Culture long_name String Culture
attribute Culture units String unitless
variable Added_Zn   byte  
attribute Added_Zn _FillValue byte 127
attribute Added_Zn actual_range byte 1, 100
attribute Added_Zn bcodmo_name String trace_metal_conc
attribute Added_Zn description String Total amount of added zinc (Zn) to incubation.
attribute Added_Zn long_name String Added Zn
attribute Added_Zn nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Added_Zn units String nanomoles per liter (nmol/L)
variable Added_Co   byte  
attribute Added_Co _FillValue byte 127
attribute Added_Co actual_range byte 1, 100
attribute Added_Co bcodmo_name String trace_metal_conc
attribute Added_Co description String Total amount of added cobalt (Co) to incubation.
attribute Added_Co long_name String Added Co
attribute Added_Co nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Added_Co units String nanomoles per liter (nmol/L)
variable Total_Zn   float  
attribute Total_Zn _FillValue float NaN
attribute Total_Zn actual_range float 1.9, 100.9
attribute Total_Zn bcodmo_name String trace_metal_conc
attribute Total_Zn description String Total zinc (Zn). Added Zn and background media Zn.
attribute Total_Zn long_name String Total Zn
attribute Total_Zn nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Total_Zn units String nanomoles per liter (nmol/L)
variable Total_Co   float  
attribute Total_Co _FillValue float NaN
attribute Total_Co actual_range float 1.1, 100.1
attribute Total_Co bcodmo_name String trace_metal_conc
attribute Total_Co description String Total cobalt (Co). Added Co and background media Co.
attribute Total_Co long_name String Total Co
attribute Total_Co nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Total_Co units String nanomoles per liter (nmol/L)
variable log_Zn2plus   float  
attribute log_Zn2plus _FillValue float NaN
attribute log_Zn2plus actual_range float -12.7, -10.99
attribute log_Zn2plus bcodmo_name String trace_metal_conc
attribute log_Zn2plus description String Log of calculated free Zn2+ ion concentration in media. [Zn2+]
attribute log_Zn2plus long_name String Log Zn2plus
attribute log_Zn2plus nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute log_Zn2plus units String moles per liter (mol/L)
variable log_Co2plus   float  
attribute log_Co2plus _FillValue float NaN
attribute log_Co2plus actual_range float -12.59, -10.63
attribute log_Co2plus bcodmo_name String trace_metal_conc
attribute log_Co2plus description String Log of calculated free Co2+ ion concentration in media. [Co2+]
attribute log_Co2plus long_name String Log Co2plus
attribute log_Co2plus nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute log_Co2plus units String moles per liter (mol/L)
variable CO_to_P_avg   float  
attribute CO_to_P_avg _FillValue float NaN
attribute CO_to_P_avg actual_range float 0.0, 0.44
attribute CO_to_P_avg bcodmo_name String unknown
attribute CO_to_P_avg description String Average ratio of cobalt (Co) in millimoles to phosphorous (P) in moles. Co (mmol):P(mol) quota.
attribute CO_to_P_avg long_name String CO To P Avg
attribute CO_to_P_avg units String dimensionless
variable ZN_to_P_avg   String  
attribute ZN_to_P_avg bcodmo_name String unknown
attribute ZN_to_P_avg description String Average ratio of zinc (Zn) in millimoles to phosphorous (P) in moles. Zn (mmol):P(mol) quota.
attribute ZN_to_P_avg long_name String ZN To P Avg
attribute ZN_to_P_avg units String dimensionless
variable CD_to_P_avg   String  
attribute CD_to_P_avg bcodmo_name String unknown
attribute CD_to_P_avg description String Average ratio of cadmium (Cd) in micromoles to phosphorous (P) in moles. Cd (umol):P(mol) quota.
attribute CD_to_P_avg long_name String CD To P Avg
attribute CD_to_P_avg units String dimensionless
variable MN_to_P_avg   float  
attribute MN_to_P_avg _FillValue float NaN
attribute MN_to_P_avg actual_range float 0.12, 4.51
attribute MN_to_P_avg bcodmo_name String unknown
attribute MN_to_P_avg description String Average ratio of manganese (Mn) in millmoles to phosphorous (P) in moles. Mn (mmol):P(mol) quota.
attribute MN_to_P_avg long_name String MN To P Avg
attribute MN_to_P_avg units String dimensionless
variable FE_to_P_avg   float  
attribute FE_to_P_avg _FillValue float NaN
attribute FE_to_P_avg actual_range float 2.21, 17.17
attribute FE_to_P_avg bcodmo_name String unknown
attribute FE_to_P_avg description String Average ratio of iron (Fe) in millmoles to phosphorous (P) in moles. Fe (mmol):P(mol) quota.
attribute FE_to_P_avg long_name String FE To P Avg
attribute FE_to_P_avg units String dimensionless
variable NI_to_P_avg   String  
attribute NI_to_P_avg bcodmo_name String unknown
attribute NI_to_P_avg description String Average ratio of nickel (Ni) in millmoles to phosphorous (P) in moles. Ni (mmol):P(mol) quota.
attribute NI_to_P_avg long_name String NI To P Avg
attribute NI_to_P_avg units String dimensionless
variable CU_to_P_avg   float  
attribute CU_to_P_avg _FillValue float NaN
attribute CU_to_P_avg actual_range float 0.062, 1.62
attribute CU_to_P_avg bcodmo_name String unknown
attribute CU_to_P_avg description String Average ratio of copper (Cu) in millmoles to phosphorous (P) in moles. Cu (mmol):P(mol) quota.
attribute CU_to_P_avg long_name String CU To P Avg
attribute CU_to_P_avg units String dimensionless
variable MO_to_P_avg   float  
attribute MO_to_P_avg _FillValue float NaN
attribute MO_to_P_avg actual_range float 0.01, 0.04
attribute MO_to_P_avg bcodmo_name String unknown
attribute MO_to_P_avg description String Average ratio of molybdenum (Mo) in millmoles to phosphorous (P) in moles. Mo (mmol):P(mol) quota.
attribute MO_to_P_avg long_name String MO To P Avg
attribute MO_to_P_avg units String dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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