ERDDAP > info > bcodmo_dataset_807299

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012		I   M	background			BCO-DMO	bcodmo_dataset_807299

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Location: Cultures collected from Northeast Pacific Line P Transect 48.8167 N 128.667 W Metal quotas Cellular metal quotas were measured by inductively coupled plasma mass spectrometry (ICP-MS). Biomass from replicate 25 mL matrix cultures of P. tricornutum CCMP632, P. delicatissima UNC1205, and Thalassiosira UNC1203 were pooled upon entering stationary phase and were centrifuged at 11,000 RPM (14,610 x g) for 40 minutes at 4\u00b0C. The cell pellet was resuspended in ~1 mL media and transferred to an acid-cleaned microcentrifuge tube. Cultures were centrifuged again for 30 min at 14,100 RPM (13,336 x g) at 4\u00b0C before the supernatant was discarded. The remaining cell pellet was acidified in 800 L of 5% nitric acid (Optima) containing 1 ppb indium for at least seven days. Solids were removed by centrifugation. No attempt was made to remove extracellular metals by washing cells with additional metal chelators in order to minimize processing blanks. Quota determinations therefore include contributions from both intracellular and extracellular pools. Process blank digestions containing acid but no cells were performed in parallel. Digests were diluted by a factor of 9 with 5% nitric acid 1 ppb indium solution before being analyzed in duplicate on a Thermo ICAP-Q plasma mass spectrometer calibrated to a multi-element standard curve (Spex Certiprep) over a range of 1 \u2013 20 ppb. Samples were analyzed in KED mode after an 85s sample uptake window and element mass windows were scanned 3 times during measurements. The 1 ppb indium internal standard was used to correct for variation in sample delivery and plasma suppression between samples. Process blanks were subtracted from measured concentrations. Phosphorus concentrations were also measured by ICP-MS simultaneously and were calibrated to a standard curve ranging from 100 \u2013 3,200 ppb using a 1 ppm certified P stock (Alfa Aesar Specpure). The seawater media base used for all growth experiments was similarly analyzed via ICP-MS using a 1:10 dilution of media base into 5% nitric acid 1 ppb indium and analyzed as above to determine background media concentrations of total Zn and Co (0.9 nmol L-1 and 0.1 nmol L-1, respectively). Missing data identifiers in this dataset include: * "nd" indicating no data * Below Detection * "contam" indicating the sample was contaminated Isolation sources and locations * Pseudonitzschia delicatissima UNC1205 and Thalassiosira UNC1203 were isolated from station P8 of the Line P transect, 48.817\uf0b0N 128.666\uf0b0W * Phaeodactylum tricornutum CCMP632 was ordered from Bigelow, the strains original location of isolation was 54\uf0b0N 4\uf0b0W * Thalassiosira pseudonana CCMP1335 was also from Bigelow, original location of isolation was 40.756\u00b0 N 72.82\u00b0 W
attribute	NC_GLOBAL	awards_0_award_nid	String	646122
attribute	NC_GLOBAL	awards_0_award_number	String	GBMF3782
attribute	NC_GLOBAL	awards_0_data_url	String	https://www.moore.org/grant-detail?grantId=GBMF3782
attribute	NC_GLOBAL	awards_0_funder_name	String	Gordon and Betty Moore Foundation: Marine Microbiology Initiative
attribute	NC_GLOBAL	awards_0_funding_acronym	String	MMI
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	385
attribute	NC_GLOBAL	awards_1_award_nid	String	785825
attribute	NC_GLOBAL	awards_1_award_number	String	OCE-1736599
attribute	NC_GLOBAL	awards_1_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736599
attribute	NC_GLOBAL	awards_1_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_1_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_1_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_1_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_1_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Line P Metal quotas PI: Mak Saito Data Version 1: 2020-03-31
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2020-03-31T20:11:49Z
attribute	NC_GLOBAL	date_modified	String	2020-04-10T14:55:44Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.807299.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/807299
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Mass Spec
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	807325
attribute	NC_GLOBAL	instruments_0_description	String	General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB16/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Mass Spectrometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	685
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Thermo ICAP-Q plasma mass spectrometer
attribute	NC_GLOBAL	keywords	String	added, Added_Co, Added_Zn, average, bco, bco-dmo, biological, CD_to_P_avg, chemical, co2plus, CO_to_P_avg, CU_to_P_avg, culture, data, dataset, dmo, erddap, FE_to_P_avg, log, log_Co2plus, log_Zn2plus, management, MN_to_P_avg, MO_to_P_avg, NI_to_P_avg, oceanography, office, preliminary, total, Total_Co, Total_Zn, zn2plus, ZN_to_P_avg
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/807299/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/807299
attribute	NC_GLOBAL	param_mapping	String	{'807299': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/807299/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Mak A. Saito
attribute	NC_GLOBAL	people_0_person_nid	String	50985
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Amber York
attribute	NC_GLOBAL	people_1_person_nid	String	643627
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	PMT Cobalt and Metalloenzymes,MM Saito
attribute	NC_GLOBAL	projects_0_acronym	String	PMT Cobalt and Metalloenzymes
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: Cobalt is important for many forms of marine life, yet it is one of the scarcest nutrients in the sea. Cobalt's oceanic abundance and distribution, along with other scarce nutrients, can influence the growth of microscopic plants (phytoplankton). This in turn can influence carbon cycles in the ocean and atmosphere. Therefore, knowledge of the controls on cobalt's abundance and chemical forms in seawater is a valuable component of our ability to understand the ocean's influence on global carbon cycling. Within phytoplankton and other marine microbes, metals such as cobalt, iron, nickel, and copper are used as critical components of enzymes responsible for key cellular reactions. Since these enzymes require metals to work, they are named metalloenzymes. Participating in a Pacific Ocean cruise from Alaska to Tahiti, this project will study the oceanic distributions of dissolved cobalt and the cellular content of a group of metalloenzymes known to influence biogeochemical cycles. The project will provide scientific impact by creating new knowledge about oceanic micronutrients in regions of economic interest with regard to fisheries and deep-sea mining. Measurement of proteins in the North Pacific will provide data of broad biological and chemical interest and will be made available through a new NSF-funded "EarthCube Ocean Protein Portal" data base. Educational impact will stem from participation of a graduate student and two young technicians, as well as the PI's development of a high school chemistry curriculum for use in two local high schools, thus allowing teachers to include real oceanic and environmental data at their first introduction to chemistry. Cobalt has a complex biogeochemical cycle. Both its inorganic and organic forms are used by biology in the upper ocean and it is removed from solution by being scavenged in the intermediate and deep ocean. This scavenging removal results in cobalt having the smallest oceanic inventory of any biologically utilized element. Recent studies, however, have found that large dissolved cobalt plumes occur in major oxygen minimum zones due to a combination of less scavenging and additions from sedimentary and remineralization fluxes. The GP15 US GEOTRACES Pacific Meridional Transect (PMT) provides an opportunity to examine the influence of oxygen depletion on cobalt chemistry. Moreover, the study of the protein component of microbial communities using new proteomic techniques will provide evidence of how different major microorganisms respond to the chemical environment (e.g. through transporter production for specific nutrients and micronutrients) as well as the biochemical basis for metal requirements related to the use of specific metalloenzymes. Specifically, the PMT provides an opportunity to confirm that the Pacific oxygen minimum zones contain a large amount of cobalt and to test the hypotheses that simultaneous zinc scarcity could induce wide-scale biochemical substitution of cobalt for zinc in the North Pacific Ocean.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-10
attribute	NC_GLOBAL	projects_0_geolocation	String	Laboratory Study and Cultures from Northeast Pacific Line P Transect 48.8167 N 128.667 W
attribute	NC_GLOBAL	projects_0_name	String	US GEOTRACES PMT: Cobalt Biogeochemical Cycling and Connections to Metalloenzymes in the Pacific Ocean
attribute	NC_GLOBAL	projects_0_project_nid	String	785826
attribute	NC_GLOBAL	projects_0_start_date	String	2017-11
attribute	NC_GLOBAL	projects_1_acronym	String	MM Saito
attribute	NC_GLOBAL	projects_1_description	String	In support of obtaining deeper knowledge of major biogeochemically relevant proteins to inform a mechanistic understanding of global marine biogeochemical cycles.
attribute	NC_GLOBAL	projects_1_end_date	String	2019-12
attribute	NC_GLOBAL	projects_1_name	String	Marine Microbial Investigator Award: Investigator Mak Saito
attribute	NC_GLOBAL	projects_1_project_nid	String	786672
attribute	NC_GLOBAL	projects_1_start_date	String	2013-05
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Metal quotas of two NE Pacific Line P diatom isolates and one Atlantic diatom isolate measured via ICP-MS after growth in zinc and cobalt media amendments. Experiments with cultures collected from the GeoMICS expedition on the R/V Thomas G. Thompson (cruise TN280), along Line P in the NE Pacific, in May of 2012.
attribute	NC_GLOBAL	title	String	Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Culture		String
attribute	Culture	bcodmo_name	String	sample_descrip
attribute	Culture	description	String	Culture name (e.g. Thalassiosira UNC12032)
attribute	Culture	long_name	String	Culture
attribute	Culture	units	String	unitless
variable	Added_Zn		byte
attribute	Added_Zn	_FillValue	byte	127
attribute	Added_Zn	actual_range	byte	1, 100
attribute	Added_Zn	bcodmo_name	String	trace_metal_conc
attribute	Added_Zn	description	String	Total amount of added zinc (Zn) to incubation.
attribute	Added_Zn	long_name	String	Added Zn
attribute	Added_Zn	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Added_Zn	units	String	nanomoles per liter (nmol/L)
variable	Added_Co		byte
attribute	Added_Co	_FillValue	byte	127
attribute	Added_Co	actual_range	byte	1, 100
attribute	Added_Co	bcodmo_name	String	trace_metal_conc
attribute	Added_Co	description	String	Total amount of added cobalt (Co) to incubation.
attribute	Added_Co	long_name	String	Added Co
attribute	Added_Co	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Added_Co	units	String	nanomoles per liter (nmol/L)
variable	Total_Zn		float
attribute	Total_Zn	_FillValue	float	NaN
attribute	Total_Zn	actual_range	float	1.9, 100.9
attribute	Total_Zn	bcodmo_name	String	trace_metal_conc
attribute	Total_Zn	description	String	Total zinc (Zn). Added Zn and background media Zn.
attribute	Total_Zn	long_name	String	Total Zn
attribute	Total_Zn	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Total_Zn	units	String	nanomoles per liter (nmol/L)
variable	Total_Co		float
attribute	Total_Co	_FillValue	float	NaN
attribute	Total_Co	actual_range	float	1.1, 100.1
attribute	Total_Co	bcodmo_name	String	trace_metal_conc
attribute	Total_Co	description	String	Total cobalt (Co). Added Co and background media Co.
attribute	Total_Co	long_name	String	Total Co
attribute	Total_Co	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	Total_Co	units	String	nanomoles per liter (nmol/L)
variable	log_Zn2plus		float
attribute	log_Zn2plus	_FillValue	float	NaN
attribute	log_Zn2plus	actual_range	float	-12.7, -10.99
attribute	log_Zn2plus	bcodmo_name	String	trace_metal_conc
attribute	log_Zn2plus	description	String	Log of calculated free Zn2+ ion concentration in media. [Zn2+]
attribute	log_Zn2plus	long_name	String	Log Zn2plus
attribute	log_Zn2plus	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	log_Zn2plus	units	String	moles per liter (mol/L)
variable	log_Co2plus		float
attribute	log_Co2plus	_FillValue	float	NaN
attribute	log_Co2plus	actual_range	float	-12.59, -10.63
attribute	log_Co2plus	bcodmo_name	String	trace_metal_conc
attribute	log_Co2plus	description	String	Log of calculated free Co2+ ion concentration in media. [Co2+]
attribute	log_Co2plus	long_name	String	Log Co2plus
attribute	log_Co2plus	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/C035/
attribute	log_Co2plus	units	String	moles per liter (mol/L)
variable	CO_to_P_avg		float
attribute	CO_to_P_avg	_FillValue	float	NaN
attribute	CO_to_P_avg	actual_range	float	0.0, 0.44
attribute	CO_to_P_avg	bcodmo_name	String	unknown
attribute	CO_to_P_avg	description	String	Average ratio of cobalt (Co) in millimoles to phosphorous (P) in moles. Co (mmol):P(mol) quota.
attribute	CO_to_P_avg	long_name	String	CO To P Avg
attribute	CO_to_P_avg	units	String	dimensionless
variable	ZN_to_P_avg		String
attribute	ZN_to_P_avg	bcodmo_name	String	unknown
attribute	ZN_to_P_avg	description	String	Average ratio of zinc (Zn) in millimoles to phosphorous (P) in moles. Zn (mmol):P(mol) quota.
attribute	ZN_to_P_avg	long_name	String	ZN To P Avg
attribute	ZN_to_P_avg	units	String	dimensionless
variable	CD_to_P_avg		String
attribute	CD_to_P_avg	bcodmo_name	String	unknown
attribute	CD_to_P_avg	description	String	Average ratio of cadmium (Cd) in micromoles to phosphorous (P) in moles. Cd (umol):P(mol) quota.
attribute	CD_to_P_avg	long_name	String	CD To P Avg
attribute	CD_to_P_avg	units	String	dimensionless
variable	MN_to_P_avg		float
attribute	MN_to_P_avg	_FillValue	float	NaN
attribute	MN_to_P_avg	actual_range	float	0.12, 4.51
attribute	MN_to_P_avg	bcodmo_name	String	unknown
attribute	MN_to_P_avg	description	String	Average ratio of manganese (Mn) in millmoles to phosphorous (P) in moles. Mn (mmol):P(mol) quota.
attribute	MN_to_P_avg	long_name	String	MN To P Avg
attribute	MN_to_P_avg	units	String	dimensionless
variable	FE_to_P_avg		float
attribute	FE_to_P_avg	_FillValue	float	NaN
attribute	FE_to_P_avg	actual_range	float	2.21, 17.17
attribute	FE_to_P_avg	bcodmo_name	String	unknown
attribute	FE_to_P_avg	description	String	Average ratio of iron (Fe) in millmoles to phosphorous (P) in moles. Fe (mmol):P(mol) quota.
attribute	FE_to_P_avg	long_name	String	FE To P Avg
attribute	FE_to_P_avg	units	String	dimensionless
variable	NI_to_P_avg		String
attribute	NI_to_P_avg	bcodmo_name	String	unknown
attribute	NI_to_P_avg	description	String	Average ratio of nickel (Ni) in millmoles to phosphorous (P) in moles. Ni (mmol):P(mol) quota.
attribute	NI_to_P_avg	long_name	String	NI To P Avg
attribute	NI_to_P_avg	units	String	dimensionless
variable	CU_to_P_avg		float
attribute	CU_to_P_avg	_FillValue	float	NaN
attribute	CU_to_P_avg	actual_range	float	0.062, 1.62
attribute	CU_to_P_avg	bcodmo_name	String	unknown
attribute	CU_to_P_avg	description	String	Average ratio of copper (Cu) in millmoles to phosphorous (P) in moles. Cu (mmol):P(mol) quota.
attribute	CU_to_P_avg	long_name	String	CU To P Avg
attribute	CU_to_P_avg	units	String	dimensionless
variable	MO_to_P_avg		float
attribute	MO_to_P_avg	_FillValue	float	NaN
attribute	MO_to_P_avg	actual_range	float	0.01, 0.04
attribute	MO_to_P_avg	bcodmo_name	String	unknown
attribute	MO_to_P_avg	description	String	Average ratio of molybdenum (Mo) in millmoles to phosphorous (P) in moles. Mo (mmol):P(mol) quota.
attribute	MO_to_P_avg	long_name	String	MO To P Avg
attribute	MO_to_P_avg	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.