ERDDAP > info > bcodmo_dataset_809321

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Rockfish hypoxia experiments] - Data from experiments testing the effects of hypoxia on behavior and physiology of two species of rockfish from from 2015-2016 (Collaborative Research: Ocean Acidification: RUI: Multiple Stressor Effects of Ocean Acidification and Hypoxia on Behavior, Physiology, and Gene Expression of Temperate Reef Fishes)		I   M	background			BCO-DMO	bcodmo_dataset_809321

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Experimental design Experiments subjecting juvenile rockfishes to simulated future DO levels were conducted at the seawater aquarium facility at the NOAA Southwest Fisheries Science Center laboratory in Santa Cruz, CA. Juvenile rockfishes were exposed to one of four treatment levels corresponding to conditions that currently occur or are predicted to occur in the future on the central California coast: 100% saturation (8.74 \u00b1 0.03 mg O2 L-1), 68% saturation (6.00 \u00b1 0.04 mg O2 L-1), 46% saturation (4.06 \u00b1 0.04 mg O2 L-1), or 26% saturation (2.25 \u00b1 0.05 mg O2 L-1), with two replicate tanks per treatment level. These levels were maintained for the duration of the experiment to simulate chronic exposure to prolonged hypoxia events and oxygen, pH, and temperature levels in all the treatment tanks were measured daily using a HACH HQ40D multiparameter meter. All experimental trials were conducted under constant temperature (12\u02daC) and pH (~8.05), with the same DO levels as the rearing tank of the fish being tested. To manipulate oxygen levels, seawater was first pumped from offshore through a series of settling tanks and sand filters and held in a 2000 L reservoir tank maintained at a constant temperature (12\u02daC) with aquarium chillers and aerated to approximately 100% air saturation (~9.0 mg O2 L-1). This source water was fed into four 500 L treatment reservoirs, which were used to create desired DO concentrations by bubbling nitrogen (N2) gas to strip O2 from the water. Gas delivery was controlled by the program WitroxView via solenoid valves, and O2 levels were monitored using Loligo Systems optical oxygen probes. Manipulated (or control) water was delivered at a rate of 20 ml s-1 to 80 L experimental tanks in a single-pass, flow-through design.\u00a0 Ten randomly assigned juvenile rockfish of each species were introduced into each of two replicate tanks for each treatment (20 fish per treatment per species). All tanks were covered to minimize visual disturbance from investigators. Sections of plastic construction fencing material measuring 90 x 120 cm were bunched together and placed in each tank to simulate kelp habitat structure. The two replicate tanks per treatment were fed on alternating days and used in experiments on non-feeding days, thereby ensuring a 36-48 hour fasting period prior to behavioral or physiological trials. Fishes were fed to satiation with frozen, high protein krill on feeding days. Prior to conducting any behavioral or physiological measurements, fish were allowed a minimum of 5 days to recover in their treatment tanks from any previous trial. See Table 1 of\u00a0Mattiasen et al. (2020) for the schedule of the various experimental trials. Escape response Escape response trials tested the time required for a fish to find the exit of an enclosed chamber (Jutfelt et al. 2013). Escape chambers were composed of a PVC tube measuring 28 cm tall x 9 cm diameter with a 5 cm diameter hole cut in the side. A slit located 8 cm from the top of the chamber allowed a black plexiglass divider to be inserted, retaining fish in the top half of the chamber during the acclimation period. Removal of the divider released the fish into the lower portion of the chamber at the start of the timed trial. Escape chambers were placed in 40 L insulated aquaria on a water table to control temperatures. A total of six replicated escape chambers and aquaria were used, allowing for six simultaneous trials. Individual fish were transferred into the top of the chamber and allowed a 15 min acclimation period. At the end of the acclimation period the divider was removed without visual interference by the investigator. Observers watched a mirror above the tanks and recorded the time at which each fish exited the chamber (defined as the time at which the head of the fish exited the chamber). Trials were terminated after 10 min regardless of whether the fish exited. Behavioral lateralization Brain functional asymmetry and behavioral lateralization reflect the bias for left vs. right turning decisions in a detour test. To measure lateralization in response to DO treatment conditions, a detour test was employed with a double T-Maze (Domenici et al., 2007). Individual fish were transferred into one end of the two-way T-Maze (50 x 30 x 25 cm L x W x H aquaria), and allowed to acclimate for 3 minutes. The starting side was alternated for every trial to minimize the potential for side bias. After the acclimation period, the fish was gently coaxed to swim down the center channel (without touching the fish) using a long PVC bar, and when it reached a barrier at the end of the channel, the fish had to decide to turn right or left. The turn direction was recorded and the experiment was repeated 10 times, 5 times in each direction. Each trial took approximately 10 minutes to complete. Absolute lateralization (LA) was calculated as LA = (|# right turns \u2013 # left turns| )/(# right turns + # left turns) x 100 as an index of non-directional turn bias. LA reflects whether turn bias exists at the population level, irrespective of direction. Relative lateralization (LR\u00ac) was also calculated to determine whether the fish in a particular treatment exhibited turning bias for a particular direction (i.e., left or right preference). LR was calculated as LR = (# right turns \u2013 # left turns)/(# right turns + # left turns) x 100 Positive values indicate a right turning bias, while negative values indicate a left turning bias. Critical oxygen tension (pCrit) A subset of 8 individuals per species from each treatment was tested for hypoxia tolerance by estimating pCrit using an automated intermittent flow respirometry system (Loligo Systems). Fish were placed in sealed respirometry chambers overnight at their treatment oxygen levels to acclimate to the chambers. Subsequently, pCrit trials were initiated by raising the oxygen saturation of the reservoir to 70% air saturation and three MO2 measurement loops (5 min flush, 10 min wait, 5 min measurement) were recorded at each oxygen level. The DO level was then reduced in a step-wise fashion by 10% air saturation, through the addition of N2 gas until reaching 40% air saturation, below which oxygen saturation was reduced at 5% intervals until reaching 10% air saturation, at which point the trial was terminated. This approach allowed us to obtain a more precise measurement of pCrit and to reduce risk of inadvertent mortality. Oxygen consumption rate (MO2 in mg O2 kg-1 hr-1) was calculated using the following equation: MO2 = \u2206PO2 V\u221dM-1 \u2206t-1 Where \u2206PO2 is the change in water partial pressure of O2 (mmHg), \u2206t is the elapsed time (h), V is the volume of the respirometer chamber minus the volume of the fish (cm3), M is the total mass of the animal (kg), and \u221d is the O2 solubility coefficient at the experimental temperature (Boutilier et al., 1988). The respirometry system was cleaned using dilute bleach after each trial to eliminate the influence of microbial respiration on subsequent trials. pCrit was calculated for each fish using the broken stick regression method (Toms and Lesperance, 2003) by computing the oxygen saturation level at which the metabolic rate began to decrease linearly with decreasing DO. Aerobic scope Aerobic scope is the difference between the standard (or resting) metabolic rate (SMR) and the maximum metabolic rate (MMR). We measured the SMR on a subset of 8 fish per species per treatment using the intermittent flow respirometry system. Four individuals at a time were weighed and placed into separate respirometer chambers, with MO2 measurements taken over a 12 hr period overnight. SMR was measured during nighttime hours to capture the MO2 at the time where the fish were at their lowest metabolic activity levels. The lowest 10% of MO2 measurements per cycle, excluding outliers (values > 2 standard deviations), were used to calculate the SMR of each individual fish (Clark et al., 2012). MMR was subsequently measured following swimming to exhaustion using a Loligo Systems 10 L swim flume (model #SW10100). Exhaustion was achieved by swimming the fish for 5 minutes at a velocity one-body length per second below the estimated average critical swimming speed of the group (N. Kashef, unpublished data). The fish were then quickly returned to the respirometry chambers and run for one measurement cycle to acquire MMR. Preliminary trials concluded that the highest MO2 values occurred directly following swimming to exhaustion. Aerobic scope was calculated by subtracting the SMR from the MMR. Ventilation rate Ventilation rate was measured on a subset of 10 individuals per species per treatment using a specially designed array of 10 experimental chambers (5 x 15 cm, water depth 4 cm), each holding an individual fish. Each chamber received flow-through seawater of the appropriate rearing DO treatment and constant temperature (12\u02daC). Following a two-hour acclimation period, two GoPro HERO4 video cameras recorded each fish for 30 minutes. Ventilation rate was determined by counting the number of open/closing cycles of the gill operculum within a minute (i.e., ventilations per minute [VPM]). Average VPM for each fish was calculated for 3 randomly selected one-minute measurements. Problem report: Some individual fish were used in each of the behavioral and physiological trials, while other fish were only tested in a subset of the possible experimental trials. In addition, if any fish died in the course of the experiments, they were replaced by a new fish.
attribute	NC_GLOBAL	awards_0_award_nid	String	532620
attribute	NC_GLOBAL	awards_0_award_number	String	EF-1416895
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=1416895
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Emerging Frontiers Division
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF EF
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	392
attribute	NC_GLOBAL	awards_0_program_manager	String	Irwin Forseth
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	520504
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Rockfish hypoxia experiments PI: Scott Hamilton (Moss Landing Marine Laboratories) Version date: 14 April 2020
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2020-04-14T19:42:57Z
attribute	NC_GLOBAL	date_modified	String	2020-04-15T19:34:41Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.809321.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/809321
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	camera
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	809358
attribute	NC_GLOBAL	instruments_0_description	String	All types of photographic equipment including stills, video, film and digital systems.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/311/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Camera
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	520
attribute	NC_GLOBAL	instruments_0_supplied_name	String	GoPro HERO4 video camera
attribute	NC_GLOBAL	instruments_1_acronym	String	Dissolved Oxygen Sensor
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	809357
attribute	NC_GLOBAL	instruments_1_description	String	An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Dissolved Oxygen Sensor
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	705
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Loligo Systems optical oxygen probes
attribute	NC_GLOBAL	instruments_2_acronym	String	Aquarium
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	809355
attribute	NC_GLOBAL	instruments_2_description	String	Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Aquarium
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	711
attribute	NC_GLOBAL	instruments_3_acronym	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	809356
attribute	NC_GLOBAL	instruments_3_description	String	Immersible or in-line liquid cooling device, usually with temperature control.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	522982
attribute	NC_GLOBAL	instruments_4_acronym	String	Hand Net
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	809353
attribute	NC_GLOBAL	instruments_4_description	String	A hand net (also called a scoop net or dip net) is a net or mesh basket held open by a hoop. They are used for scooping fish near the surface of the water.
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Hand Net
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	682469
attribute	NC_GLOBAL	instruments_4_supplied_name	String	large mesh hand nets
attribute	NC_GLOBAL	instruments_5_acronym	String	SCUBA
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	809354
attribute	NC_GLOBAL	instruments_5_description	String	The self-contained underwater breathing apparatus or scuba diving system is the result of technological developments and innovations that began almost 300 years ago. Scuba diving is the most extensively used system for breathing underwater by recreational divers throughout the world and in various forms is also widely used to perform underwater work for military, scientific, and commercial purposes. Reference: http://oceanexplorer.noaa.gov/technology/diving/diving.html
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Self-Contained Underwater Breathing Apparatus
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	713363
attribute	NC_GLOBAL	instruments_5_supplied_name	String	SCUBA
attribute	NC_GLOBAL	keywords	String	absolute, Absolute_Lateralization_Score, aerobic, Aerobic_Scope_mg_O2_per_kg_per_hour, air, bco, bco-dmo, beats, biological, chemical, data, dataset, date, Date_at_end_of_experiment, Date_entered_into_experimental_treatments, dmo, end, entered, erddap, escape, Escape_Time_seconds, experiment, experimental, final, Final_Standard_Length_mm, Final_Total_Length_mm, Final_Weight_g, fish, Fish_ID, hour, initial, Initial_Standard_Length_mm, Initial_Total_Length_mm, Initial_Weight_g, into, lateralization, length, management, maximum, Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour, metabolic, minute, O2, oceanography, office, operculum, oxygen, Oxygen_treatment_mg_per_L, pcnt, pcrit, Pcrit_pcnt_air_saturation, per, preliminary, rate, relative, Relative_Lateralization_Score, replicate, saturation, scope, score, seconds, species, standard, Standard_Metabolic_Rate_mg_O2_per_kg_per_hour, tank, Tank_Replicate, time, total, treatment, treatments, ventilation, Ventilation_rate_operculum_beats_per_minute, weight
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/809321/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/809321
attribute	NC_GLOBAL	param_mapping	String	{'809321': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/809321/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Moss Landing Marine Laboratories
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	MLML
attribute	NC_GLOBAL	people_0_person_name	String	Scott Hamilton
attribute	NC_GLOBAL	people_0_person_nid	String	516017
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	OA Hypoxia Rockfish
attribute	NC_GLOBAL	projects_0_acronym	String	OA Hypoxia Rockfish
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: For near shore marine species inhabiting upwelling ecosystems such as the California Current, climate change resulting from the anthropogenic release of CO2 into the atmosphere is likely to induce concurrent conditions of ocean acidification (OA) and hypoxia, which are exacerbated during periods of seasonal upwelling. Although marine fishes have generally been presumed to be tolerant of OA due to their competence in acid-base regulation, recent studies in tropical regions suggest that early life stages may be particularly sensitive to elevated levels of dissolved CO2 (which lowers seawater pH) by impairing respiration, acid-base regulation, and neurotransmitter function. Low levels of dissolved oxygen (DO), which occur during hypoxia, can likewise impact the behavior, physiology and survival of marine fishes. Few studies have addressed the potential interactive effects of a low pH, low DO environment. From molecular tools to whole animal physiology, this research will provide an in-depth examination of an inherently integrative process. The study will use a multiple stressor framework to address the potential threats posed by the independent and combined effects of OA and hypoxia on behavior, physiological capacity, and gene expression in temperate reef fishes. Because mortality in early life stages has important carryover effects, understanding the effects of these stressors is critical for predicting future climate change responses of global fish populations. Such information will lay the groundwork for further studies that address the synergistic effects of multiple stressors and the characteristics of California Current species that influence their ability to tolerate or adapt to changes in ocean chemistry in a rapidly changing climate. The project goals are to use a combination of laboratory and field studies to examine ecologically and physiologically relevant responses of juvenile rockfish (genus Sebastes) to the independent and interactive effects of ocean acidification and hypoxia. Rockfish will be captured in the field and then reared in the lab at 4 different pCO2 levels and 4 different DO levels to simulate changes in environmental conditions. Response variables include:  (1) measures of changes in olfactory capabilities, brain functional asymmetry and problem-solving ability and (2) effects on swimming capabilities, respiration, aerobic performance, and growth. In addition, we will use next generation transcriptome sequencing to examine genome-wide changes in gene expression and enzyme activity for Na+/K+ ATPase (NKA), citrate synthase (CS), and lactate dehydrogenase (LDH), as proxies for acid-base compensation and metabolic shifts between aerobic and anaerobic metabolism. Oceanographic sensors will be deployed in the field to determine the frequency and intensity of hypoxia and low pH events in near shore habitats in Northern and Central California. Adaptive sampling of juvenile rockfish will be used to evaluate gene expression and physiological responses in individuals exposed in situ to low pH and low DO events in the field. The effects of OA and hypoxia will be compared across rockfish species with different life histories (e.g. larval duration, timing of spawning, etc.) and collected from regions differing in exposure to low pH/low DO events to address the potential for local adaptation. The focus of this project is on responses of the early juvenile stage at the time of settlement, because this stage is exposed to near shore changes in ocean chemistry during a critical period where physiological stress and behavioral disruptions may have the strongest demographic effects due to increased risk of predation.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-08
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Ocean Acidification: RUI: Multiple Stressor Effects of Ocean Acidification and Hypoxia on Behavior, Physiology, and Gene Expression of Temperate Reef Fishes
attribute	NC_GLOBAL	projects_0_project_nid	String	516018
attribute	NC_GLOBAL	projects_0_start_date	String	2014-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This study investigated the effects of hypoxia on the behavior and physiology of juvenile rockfishes in a controlled laboratory setting to test how deoxygenation may impact early life stages of common temperate reef fishes. Juvenile rockfishes were collected from shallow rocky reef and kelp forest habitats at Stillwater Cove, central California (36\u02da 34' N, 121\u02da 56' W) during May-June of 2015. Newly settled copper (Sebastes caurinus) and blue (Sebastes mystinus) rockfish were reared in the laboratory under across a range of oxygen concentrations. Juvenile rockfishes were exposed to one of four dissolved oxygen treatment levels corresponding to conditions that currently occur or are predicted to occur in the future on the central California coast: 100% saturation (8.74 \u00b1 0.03 mg O2 L-1), 68% saturation (6.00 \u00b1 0.04 mg O2 L-1), 46% saturation (4.06 \u00b1 0.04 mg O2 L-1), or 26% saturation (2.25 \u00b1 0.05 mg O2 L-1), with two replicate tanks per treatment level. Behavior and physiological trials were conducted on each individual to test how each species responds to declining oxygen levels, including (1) escape response, (2) behavioral lateralization, (3) standard metabolic rate, (4) maximum metabolic rate, (5) aerobic scope, (6) pCrit (i.e., hypoxia tolerance test), and (7) ventilation rate. These data are published in Mattiasen et al. (2020).
attribute	NC_GLOBAL	title	String	[Rockfish hypoxia experiments] - Data from experiments testing the effects of hypoxia on behavior and physiology of two species of rockfish from from 2015-2016 (Collaborative Research: Ocean Acidification: RUI: Multiple Stressor Effects of Ocean Acidification and Hypoxia on Behavior, Physiology, and Gene Expression of Temperate Reef Fishes)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Fish_ID		float
attribute	Fish_ID	_FillValue	float	NaN
attribute	Fish_ID	actual_range	float	1.0, 220.0
attribute	Fish_ID	bcodmo_name	String	individual
attribute	Fish_ID	description	String	Unique identifer
attribute	Fish_ID	long_name	String	Fish ID
attribute	Fish_ID	units	String	unitless
variable	Species		String
attribute	Species	bcodmo_name	String	species
attribute	Species	description	String	Species used in the experiments
attribute	Species	long_name	String	Species
attribute	Species	units	String	unitless
variable	Oxygen_treatment_mg_per_L		float
attribute	Oxygen_treatment_mg_per_L	_FillValue	float	NaN
attribute	Oxygen_treatment_mg_per_L	actual_range	float	2.2, 8.4
attribute	Oxygen_treatment_mg_per_L	bcodmo_name	String	treatment
attribute	Oxygen_treatment_mg_per_L	description	String	Experimental treatment condition
attribute	Oxygen_treatment_mg_per_L	long_name	String	Oxygen Treatment Mg Per L
attribute	Oxygen_treatment_mg_per_L	units	String	milligrams O2 per liter (mg/L)
variable	Tank_Replicate		String
attribute	Tank_Replicate	bcodmo_name	String	replicate
attribute	Tank_Replicate	description	String	Two replicate tanks (A and B) per treatment
attribute	Tank_Replicate	long_name	String	Tank Replicate
attribute	Tank_Replicate	units	String	unitless
variable	Initial_Standard_Length_mm		float
attribute	Initial_Standard_Length_mm	_FillValue	float	NaN
attribute	Initial_Standard_Length_mm	actual_range	float	29.0, 56.3
attribute	Initial_Standard_Length_mm	bcodmo_name	String	length
attribute	Initial_Standard_Length_mm	description	String	Initial length measured from tip of mouth to hypural bones
attribute	Initial_Standard_Length_mm	long_name	String	Initial Standard Length Mm
attribute	Initial_Standard_Length_mm	units	String	millimeters (mm)
variable	Initial_Total_Length_mm		float
attribute	Initial_Total_Length_mm	_FillValue	float	NaN
attribute	Initial_Total_Length_mm	actual_range	float	36.2, 67.6
attribute	Initial_Total_Length_mm	bcodmo_name	String	length
attribute	Initial_Total_Length_mm	description	String	Initial length measured from tip of mouth to end of caudal fin
attribute	Initial_Total_Length_mm	long_name	String	Initial Total Length Mm
attribute	Initial_Total_Length_mm	units	String	millimeters (mm)
variable	Initial_Weight_g		float
attribute	Initial_Weight_g	_FillValue	float	NaN
attribute	Initial_Weight_g	actual_range	float	0.411, 3.272
attribute	Initial_Weight_g	bcodmo_name	String	weight
attribute	Initial_Weight_g	description	String	Initial weight
attribute	Initial_Weight_g	long_name	String	Initial Weight G
attribute	Initial_Weight_g	units	String	grams (g)
variable	Date_entered_into_experimental_treatments		String
attribute	Date_entered_into_experimental_treatments	bcodmo_name	String	date
attribute	Date_entered_into_experimental_treatments	description	String	Date entered into experimental treatments; format: yyyy-mm-dd
attribute	Date_entered_into_experimental_treatments	long_name	String	Date Entered Into Experimental Treatments
attribute	Date_entered_into_experimental_treatments	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date_entered_into_experimental_treatments	units	String	unitless
variable	Date_at_end_of_experiment		String
attribute	Date_at_end_of_experiment	bcodmo_name	String	date
attribute	Date_at_end_of_experiment	description	String	Date at end of experiment; format: yyyy-mm-dd
attribute	Date_at_end_of_experiment	long_name	String	Date At End Of Experiment
attribute	Date_at_end_of_experiment	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date_at_end_of_experiment	units	String	unitless
variable	Final_Standard_Length_mm		float
attribute	Final_Standard_Length_mm	_FillValue	float	NaN
attribute	Final_Standard_Length_mm	actual_range	float	41.9, 73.5
attribute	Final_Standard_Length_mm	bcodmo_name	String	length
attribute	Final_Standard_Length_mm	description	String	Final length measured from tip of mouth to hypural bones
attribute	Final_Standard_Length_mm	long_name	String	Final Standard Length Mm
attribute	Final_Standard_Length_mm	units	String	millimeters (mm)
variable	Final_Total_Length_mm		float
attribute	Final_Total_Length_mm	_FillValue	float	NaN
attribute	Final_Total_Length_mm	actual_range	float	51.2, 86.8
attribute	Final_Total_Length_mm	bcodmo_name	String	length
attribute	Final_Total_Length_mm	description	String	Final length measured from tip of mouth to end of caudal fin
attribute	Final_Total_Length_mm	long_name	String	Final Total Length Mm
attribute	Final_Total_Length_mm	units	String	millimeters (mm)
variable	Final_Weight_g		float
attribute	Final_Weight_g	_FillValue	float	NaN
attribute	Final_Weight_g	actual_range	float	1.12, 548.0
attribute	Final_Weight_g	bcodmo_name	String	weight
attribute	Final_Weight_g	description	String	Final weight
attribute	Final_Weight_g	long_name	String	Final Weight G
attribute	Final_Weight_g	units	String	grams (g)
variable	Escape_Time_seconds		short
attribute	Escape_Time_seconds	_FillValue	short	32767
attribute	Escape_Time_seconds	actual_range	short	3, 2100
attribute	Escape_Time_seconds	bcodmo_name	String	time_elapsed
attribute	Escape_Time_seconds	description	String	Time required for fish to exit the chamber
attribute	Escape_Time_seconds	long_name	String	Escape Time Seconds
attribute	Escape_Time_seconds	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	Escape_Time_seconds	units	String	seconds
variable	Relative_Lateralization_Score		byte
attribute	Relative_Lateralization_Score	_FillValue	byte	127
attribute	Relative_Lateralization_Score	actual_range	byte	-100, 100
attribute	Relative_Lateralization_Score	bcodmo_name	String	sample_descrip
attribute	Relative_Lateralization_Score	description	String	Percent of time fish turned right (positive score) or left (negative score)
attribute	Relative_Lateralization_Score	long_name	String	Relative Lateralization Score
attribute	Relative_Lateralization_Score	units	String	unitless (percent)
variable	Absolute_Lateralization_Score		byte
attribute	Absolute_Lateralization_Score	_FillValue	byte	127
attribute	Absolute_Lateralization_Score	actual_range	byte	0, 100
attribute	Absolute_Lateralization_Score	bcodmo_name	String	sample_descrip
attribute	Absolute_Lateralization_Score	description	String	Percent of time fish showed a turn bias out of 10 trials
attribute	Absolute_Lateralization_Score	long_name	String	Absolute Lateralization Score
attribute	Absolute_Lateralization_Score	units	String	unitless (percent)
variable	Ventilation_rate_operculum_beats_per_minute		double
attribute	Ventilation_rate_operculum_beats_per_minute	_FillValue	double	NaN
attribute	Ventilation_rate_operculum_beats_per_minute	actual_range	double	18.33333333, 90.0
attribute	Ventilation_rate_operculum_beats_per_minute	bcodmo_name	String	respiration
attribute	Ventilation_rate_operculum_beats_per_minute	description	String	Average number of breaths per minute
attribute	Ventilation_rate_operculum_beats_per_minute	long_name	String	Ventilation Rate Operculum Beats Per Minute
attribute	Ventilation_rate_operculum_beats_per_minute	units	String	breaths per minute (BPM)
variable	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour		float
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	_FillValue	float	NaN
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	actual_range	float	52.0, 197.27
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	bcodmo_name	String	O2 consumption
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	description	String	Oxygen consumption of fish at rest
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	long_name	String	Standard Metabolic Rate Mg O2 Per Kg Per Hour
attribute	Standard_Metabolic_Rate_mg_O2_per_kg_per_hour	units	String	milligrams O2 per kilogram per hour
variable	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour		float
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	_FillValue	float	NaN
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	actual_range	float	101.82, 361.0
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	bcodmo_name	String	O2 consumption
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	description	String	Oxygen consumption of fish after exercise
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	long_name	String	Maximum Metabolic Rate Mg O2 Per Kg Per Hour
attribute	Maximum_Metabolic_Rate_mg_O2_per_kg_per_hour	units	String	milligrams O2 per kilogram per hour
variable	Aerobic_Scope_mg_O2_per_kg_per_hour		float
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	_FillValue	float	NaN
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	actual_range	float	6.37, 227.83
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	bcodmo_name	String	O2 consumption
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	description	String	Difference between standard and maximum metabolic rate
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	long_name	String	Aerobic Scope Mg O2 Per Kg Per Hour
attribute	Aerobic_Scope_mg_O2_per_kg_per_hour	units	String	milligrams O2 per kilogram per hour
variable	Pcrit_pcnt_air_saturation		float
attribute	Pcrit_pcnt_air_saturation	_FillValue	float	NaN
attribute	Pcrit_pcnt_air_saturation	actual_range	float	12.4595, 34.3444
attribute	Pcrit_pcnt_air_saturation	bcodmo_name	String	O2sat
attribute	Pcrit_pcnt_air_saturation	description	String	Measure of hypoxia tolerance as percent air saturation
attribute	Pcrit_pcnt_air_saturation	long_name	String	Pcrit Pcnt Air Saturation
attribute	Pcrit_pcnt_air_saturation	units	String	unitless (percent)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.