ERDDAP > info > bcodmo_dataset_817436

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Sponge-Associated Microbial Communities (via 16S-V4 rRNA amplicon sequencing) Following Storm-Driven Flooding] - SRA accessions and collection information for 16S-V4 rRNA amplicon data from invertebrates sampled at Flower Garden Banks National Marine Sanctuary, Gulf of Mexico following Tax Day Flooding (2016), Hurricane Harvey (2017), and a no flooding year (2018) (RAPID: Collaborative Research: Impact of freshwater runoff from Hurricane Harvey on coral reef benthic organisms and associated microbial communities)		F   I   M	background			BCO-DMO	bcodmo_dataset_817436

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	Location: East and West Banks of the Flower Garden Banks National Marine Sanctuary (FGBNMS)\u00a0 Sampling Events:\u00a0 NOAA FGBNMS Cruise July 2016, NOAA FGBNMS Cruise August 2016 Hurricane Harvey FGB October 2017, Hurricane Harvey FGB October 2018 Methodology: V4-16S bacterial communities libraries were prepared and PE 250bp reads were generated using Illumina MiSeq platform.\u00a0 Sampling and analytical procedures: Samples were flash frozen in liquid nitrogen and stored at -20\u2103 until further processing. DNA was extracted from 250 mg of sponge sample using the Nucleospin Soil DNA extraction kit (Takara Bio) or the DNeasy PowerSoil DNA extraction kit (QIAGEN).\u00a0\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	746813
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1800914
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1800914
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Daniel Thornhill
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	722161
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Sponge-Associated Microbial Communities (via 16S-V4 rRNA amplicon sequencing) Following Storm-Driven Flooding PI: Adrienne Simoes Correa Data Version 1: 2020-07-23
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2020-06-30T20:47:45Z
attribute	NC_GLOBAL	date_modified	String	2020-07-24T18:14:00Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.817436.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-93.6002
attribute	NC_GLOBAL	geospatial_lat_max	double	27.9078
attribute	NC_GLOBAL	geospatial_lat_min	double	27.8819
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-93.6002
attribute	NC_GLOBAL	geospatial_lon_min	double	-93.62829
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	28.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	16.5
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/817436
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Automated Sequencer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	817437
attribute	NC_GLOBAL	instruments_0_description	String	General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Automated DNA Sequencer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	649
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Illumina MiSeq Platform
attribute	NC_GLOBAL	keywords	String	accession, bank, bco, bco-dmo, biological, chemical, collection, Collection_Date_End, data, dataset, date, depth, Depth_max, Depth_min, dmo, end, erddap, latitude, longitude, management, name, oceanography, office, preliminary, sample, Sample_Name, species, sra, start, time
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/817436/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/817436
attribute	NC_GLOBAL	Northernmost_Northing	double	27.9078
attribute	NC_GLOBAL	param_mapping	String	{'817436': {'Lat': 'master - latitude', 'Depth_max': 'master - depth', 'Long': 'master - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/817436/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Rice University
attribute	NC_GLOBAL	people_0_person_name	String	Adrienne Simoes Correa
attribute	NC_GLOBAL	people_0_person_nid	String	713331
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Houston
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UH-Clearlake
attribute	NC_GLOBAL	people_1_person_name	String	Lory Santiago-Vazquez
attribute	NC_GLOBAL	people_1_person_nid	String	746825
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Rice University
attribute	NC_GLOBAL	people_2_person_name	String	Amanda Shore
attribute	NC_GLOBAL	people_2_person_nid	String	817444
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Amber D. York
attribute	NC_GLOBAL	people_3_person_nid	String	643627
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Rapid Reefs Harvey
attribute	NC_GLOBAL	projects_0_acronym	String	Rapid Reefs Harvey
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Coral reefs are ecologically and economically important ecosystems, and are threatened by a variety of global (climate change) and local (overfishing, pollution) stressors. Anthropogenic climate change is increasing the frequency and severity of storms, which can physically damage reef structures and reduce reef health through changes in seawater quality. In August of 2017, Hurricane Harvey caused widespread flooding in southeast Texas when it released more than 50 trillion liters of rain, which then accumulated along the Texas Shelf. This runoff is expected to impact nearby coral reefs in the Flower Garden Banks National Marine Sanctuary (FGBNMS, northwest Gulf of Mexico) via eddies and jets that transport coastal waters offshore. Findings from this project will allow managers to quickly predict whether extreme storm events are likely to induce reef mortality and ecosystem decline due to freshwater accumulation, by tracking of low salinity water masses coupled with microbial community characterization and metrics of coral health. These data are critical to managing coastal ecosystems, including the high coral cover reefs in the FGBNMS, and will help stakeholders (e.g., diving and fishing communities) plan for and minimize disruption to their livelihoods following these storms. Results will be communicated broadly across scientific arenas, in graduate and undergraduate education and training programs, and to the general public through outreach. The investigators have seven 7 square meter 2-D Reef Replicas from 2014 depicting representative FGBNMS reef bottoms, and will construct additional 2-D Reef Replicas from both banks following the arrival of Harvey runoff, allowing the public to directly experience and quantify the effects of Hurricane Harvey on local reefs using quadrats and identification guides. This project will also synergize with NSF REU programs at Boston University and Texas A&M University, providing transformative research experiences for undergraduates. One post-doctoral scholar, four graduate students, a technician and more than 5 undergraduates will be involved in all aspects of the research. All datasets will be made freely available to the public, and will serve as an important set of baselines for future lines of inquiry into the processes by which hurricanes and other extreme storms impact reef health. Hurricanes and other extreme storm events can decimate coral reefs through wave-driven physical damage. Freshwater runoff from extreme storms is also potentially detrimental to reefs but has received comparatively less attention. This research will provide unprecedented resolution on how hurricanes and other extreme storm events may trigger cascading interactions among water chemistry, declines in metazoan health and shifts in their associated microbial communities, ultimately resulting in coral reef decline. The freshwater runoff initiated by Hurricane Harvey is likely to impact reefs within the FGBNMS, one of the few remaining coral-dominated reefs in the greater Caribbean. The effects of Harvey runoff will be compared to a previously documented storm-driven runoff event that was associated with invertebrate mortality on the same reef system. Sampling seawater chemistry, microbial communities (water column and benthic), and host gene expression and proteomics before, immediately after, and six months after Harvey runoff enters the FGBNMS will allow us to identify commonalities among large-scale freshwater runoff events and track the response of benthic invertebrate health, microbial community diversity, and the trajectory of reef community recovery or decline. The investigators will determine if changes in water chemistry induce pelagic microbial shifts, if microbial communities typically associated with corals and sponges are altered, and whether feedbacks occur between these potential drivers of benthic invertebrate mortality.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-11
attribute	NC_GLOBAL	projects_0_geolocation	String	Flower Garden Banks National Marine Sanctuary, Northwest Gulf of Mexico
attribute	NC_GLOBAL	projects_0_name	String	RAPID: Collaborative Research: Impact of freshwater runoff from Hurricane Harvey on coral reef benthic organisms and associated microbial communities
attribute	NC_GLOBAL	projects_0_project_nid	String	746814
attribute	NC_GLOBAL	projects_0_start_date	String	2017-12
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	27.8819
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	To document the effects of storm-driven freshwater runoff on\u00a0sponge- associated microbiomes, we leveraged the heavy rainfall\u00a0associated with Tax Day Flooding (July 2016) and Hurricane Harvey\u00a0(August 2017) to characterize sponge-associated bacterial communities\u00a0at five time points: in July 2016 (at detection of the mortality\u00a0event), one month after the mortality event (August 2016), immediately\u00a0after Hurricane Harvey (September 2017), one month after Hurricane\u00a0Harvey (October 2017), and approximately one year following Hurricane\u00a0Harvey (October 2018). These data contain Sequence Read Archive (SRA) and BioSample accession numbers associated with BioProject\u00a0PRJNA605902 (see\u00a0[https://www.ncbi.nlm.nih.gov/bioproject/605902](\\https://www.ncbi.nlm.nih.gov/bioproject/605902\\))\u00a0at The National Center for Biotechnology Information.
attribute	NC_GLOBAL	title	String	[Sponge-Associated Microbial Communities (via 16S-V4 rRNA amplicon sequencing) Following Storm-Driven Flooding] - SRA accessions and collection information for 16S-V4 rRNA amplicon data from invertebrates sampled at Flower Garden Banks National Marine Sanctuary, Gulf of Mexico following Tax Day Flooding (2016), Hurricane Harvey (2017), and a no flooding year (2018) (RAPID: Collaborative Research: Impact of freshwater runoff from Hurricane Harvey on coral reef benthic organisms and associated microbial communities)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-93.62829
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	Sample_Name		String
attribute	Sample_Name	bcodmo_name	String	sample
attribute	Sample_Name	description	String	Sample name
attribute	Sample_Name	long_name	String	Sample Name
attribute	Sample_Name	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Sample_Name	units	String	unitless
variable	Collection_Date_Start		String
attribute	Collection_Date_Start	bcodmo_name	String	date
attribute	Collection_Date_Start	description	String	Sample collection date (start of date range) in ISO 8601 format yyyy-mm-dd
attribute	Collection_Date_Start	long_name	String	Collection Date Start
attribute	Collection_Date_Start	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Collection_Date_Start	source_name	String	Collection_Date_Start
attribute	Collection_Date_Start	time_precision	String	1970-01-01
attribute	Collection_Date_Start	units	String	unitless
variable	Collection_Date_End		String
attribute	Collection_Date_End	bcodmo_name	String	date
attribute	Collection_Date_End	description	String	Sample collection date (end of date range) in ISO 8601 format yyyy-mm-dd
attribute	Collection_Date_End	long_name	String	Collection Date End
attribute	Collection_Date_End	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Collection_Date_End	time_precision	String	1970-01-01
attribute	Collection_Date_End	units	String	unitless
variable	Depth_min		float
attribute	Depth_min	_FillValue	float	NaN
attribute	Depth_min	actual_range	float	16.5, 28.0
attribute	Depth_min	bcodmo_name	String	depth
attribute	Depth_min	colorBarMaximum	double	8000.0
attribute	Depth_min	colorBarMinimum	double	-8000.0
attribute	Depth_min	colorBarPalette	String	TopographyDepth
attribute	Depth_min	description	String	Sample collection depth (minimum of depth range)
attribute	Depth_min	long_name	String	Depth
attribute	Depth_min	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	Depth_min	standard_name	String	depth
attribute	Depth_min	units	String	meters (m)
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	16.5, 28.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	Sample collection depth (maxiumum of depth range)
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	Bank		String
attribute	Bank	bcodmo_name	String	site_descrip
attribute	Bank	description	String	East or west bank of the Flower Garden Banks
attribute	Bank	long_name	String	Bank
attribute	Bank	units	String	unitless
variable	Species		String
attribute	Species	bcodmo_name	String	taxon
attribute	Species	description	String	Identification (taxonomic name or organism description)
attribute	Species	long_name	String	Species
attribute	Species	units	String	unitless
variable	SRA		String
attribute	SRA	bcodmo_name	String	accession number
attribute	SRA	description	String	Sequence Read Archive (SRA) accession number at NCBI
attribute	SRA	long_name	String	SRA
attribute	SRA	units	String	unitless
variable	Accession		String
attribute	Accession	bcodmo_name	String	accession number
attribute	Accession	description	String	BioSample accession number at NCBI
attribute	Accession	long_name	String	Accession
attribute	Accession	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	27.8819, 27.9078
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Sample latitude
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-93.62829, -93.6002
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Sample longitude
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	source_name	String	Long
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.