ERDDAP > info > bcodmo_dataset_929459_v1

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Synechococcus DOP Displacement Experiment] - Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)		M	background			BCO-DMO	bcodmo_dataset_929459_v1

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.929459.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/929459
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	license	String	The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	summary	String	The affinity of Synechococcus (WH8102 and WH5701) alkaline phosphatases for different DOP model substrates was examined in laboratory experiments through its ability to inhibit the hydrolysis of the fluorogenic substrate 4-methylumbelliferyl phosphate (MUF-P). These data were collected as part of a study of "Dissolved organic phosphorus bond-class utilization by Synechococcus" (Waggoner et al., 2024). Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
attribute	NC_GLOBAL	title	String	[Synechococcus DOP Displacement Experiment] - Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)
variable	synechococcus_strain		String
attribute	synechococcus_strain	long_name	String	Synechococcus_strain
attribute	synechococcus_strain	units	String	unitless
variable	MUF_P_conc		float
attribute	MUF_P_conc	actual_range	float	0.5, 0.5
attribute	MUF_P_conc	long_name	String	Muf_p_conc
attribute	MUF_P_conc	units	String	micromoles per liter (umol L-1)
variable	DOP_conc		int
attribute	DOP_conc	actual_range	int	0, 100
attribute	DOP_conc	long_name	String	Dop_conc
attribute	DOP_conc	units	String	micromoles per liter (umol L-1)
variable	DOP_substrate		String
attribute	DOP_substrate	long_name	String	Dop_substrate
attribute	DOP_substrate	units	String	unitless
variable	MUFP_hydrolysis_trip1		float
attribute	MUFP_hydrolysis_trip1	actual_range	float	0.0, 193.0
attribute	MUFP_hydrolysis_trip1	long_name	String	Mufp_hydrolysis_trip1
attribute	MUFP_hydrolysis_trip1	units	String	percentage (%)
variable	MUFP_hydrolysis_trip2		float
attribute	MUFP_hydrolysis_trip2	actual_range	float	28.0, 182.0
attribute	MUFP_hydrolysis_trip2	long_name	String	Mufp_hydrolysis_trip2
attribute	MUFP_hydrolysis_trip2	units	String	percentage (%)
variable	MUFP_hydrolysis_trip3		float
attribute	MUFP_hydrolysis_trip3	actual_range	float	20.0, 184.0
attribute	MUFP_hydrolysis_trip3	long_name	String	Mufp_hydrolysis_trip3
attribute	MUFP_hydrolysis_trip3	units	String	percentage (%)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.