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     data   graph     files  public [Mussel gene expression] - Gene expression data from mussels (Mytilus edulis) subjected to
stable versus fluctuating temperatures from laboratory experiments conducted in
2023 (Collaborative Research: Microscale interactions of foundation species with their fluid
environment: biological feedbacks alter ecological interactions of mussels)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.953794.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/953794 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL summary String We measured gene expression levels of blue mussels (Mytilus edulis Linneaus, 1758) under stable versus fluctuating water temperatures. Mussels were obtained from the intertidal zone at Black Point in Narragansett Bay, RI, USA (41° 24′ 4.4964″ N, 71° 27′ 43.8228″ W) and shipped in chilled coolers overnight to the lab at Carleton College in Northfild MN. Mussels were acclimated in a recirculating seawater (Instant Ocean, Blacksburg, VA, USA) tank system (Aquaneering Inc., San Marcos, CA, USA) for 2-3 weeks at 14-16°C and fed commercial Shellfish Diet 1800 (Reed Mariculture, Campbell, CA, USA) at a rate of 5% dry mussel tissue weight day-1

During our experiment, mussels were maintained for five days under one of three stable temperature treatments (e.g., 15, 20, or 25°C) or under a fluctuating temperature treatment between 15 and 25°C. On each day of the experiment, gill tissue from three mussels per temperature treatment were sampled to test for the expression of stress response (hsp70) and cellular respiration (NADH) related genes.
attribute NC_GLOBAL title String [Mussel gene expression] - Gene expression data from mussels (Mytilus edulis) subjected to stable versus fluctuating temperatures from laboratory experiments conducted in 2023 (Collaborative Research: Microscale interactions of foundation species with their fluid environment: biological feedbacks alter ecological interactions of mussels)
variable Species   String  
attribute Species long_name String Species
attribute Species units String unitless
variable date_local   String  
attribute date_local long_name String Date_local
attribute date_local units String unitless
variable Day   int  
attribute Day actual_range int 1, 6
attribute Day long_name String Day
attribute Day units String unitless
variable Temperature   String  
attribute Temperature long_name String Temperature
attribute Temperature units String unitless
variable QUBIT_QUANT   float  
attribute QUBIT_QUANT actual_range float 50.2, 1100.0
attribute QUBIT_QUANT long_name String Qubit_quant
attribute QUBIT_QUANT units String nanograms per microliter (ng/uL)
variable QUBIT_QUAL   float  
attribute QUBIT_QUAL actual_range float 3.8, 10.0
attribute QUBIT_QUAL long_name String Qubit_qual
attribute QUBIT_QUAL units String unitless
variable NANO_QUANT   float  
attribute NANO_QUANT actual_range float 25.2, 1540.1
attribute NANO_QUANT long_name String Nano_quant
attribute NANO_QUANT units String nanograms per microliter (ng/uL)
variable A260_to_280   float  
attribute A260_to_280 actual_range float 1.63, 2.18
attribute A260_to_280 long_name String A260_to_280
attribute A260_to_280 units String unitless
variable delta_delta_Ct   float  
attribute delta_delta_Ct actual_range float 0.432397, 9.03227
attribute delta_delta_Ct long_name String Delta_delta_ct
attribute delta_delta_Ct units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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