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     data   graph     files  public [Combined Selfish Bacteria Cell Counts 2023-2024] - Cell counts exhibiting 'Selfish' uptake
in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the
Eastern Coast of Japan under varying hydrostatic pressures, 2023-2024 (Collaborative Research:
Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.963393.1
attribute NC_GLOBAL Easternmost_Easting double 141.8606
attribute NC_GLOBAL geospatial_lat_max double 55.975
attribute NC_GLOBAL geospatial_lat_min double 34.0126
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 141.8606
attribute NC_GLOBAL geospatial_lon_min double -73.0271
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 5200.0
attribute NC_GLOBAL geospatial_vertical_min double 10.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://osprey.bco-dmo.org/dataset/963393 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL Northernmost_Northing double 55.975
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 34.0126
attribute NC_GLOBAL summary String Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2022), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).
 
Through our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean, in Danish Coastal Seawater, and abyssopelagic waters off the Eastern Coast of Japan.
 
Here we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting 'selfish' uptake, and total cellular abundance.
attribute NC_GLOBAL time_coverage_end String 2024-05-22T15:12:00Z
attribute NC_GLOBAL time_coverage_start String 2023-10-11T01:34:03Z
attribute NC_GLOBAL title String [Combined Selfish Bacteria Cell Counts 2023-2024] - Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan under varying hydrostatic pressures, 2023-2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean)
attribute NC_GLOBAL Westernmost_Easting double -73.0271
variable deployment   String  
attribute deployment long_name String Deployment
attribute deployment units String unitless
variable station   String  
attribute station long_name String Station
attribute station units String unitless
variable latitude   float  
attribute latitude _CoordinateAxisType String Lat
attribute latitude actual_range float 34.0126, 55.975
attribute latitude axis String Y
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude_n
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   float  
attribute longitude _CoordinateAxisType String Lon
attribute longitude actual_range float -73.0271, 141.8606
attribute longitude axis String X
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude_e
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.696988043E9, 1.71639072E9
attribute time axis String T
attribute time ioos_category String Time
attribute time long_name String Iso_datetime_utc
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z
variable date   String  
attribute date long_name String Date
attribute date units String unitless
variable time_local   String  
attribute time_local long_name String Time_local
attribute time_local units String unitless
variable cast_number   String  
attribute cast_number long_name String Cast_number
attribute cast_number units String unitless
variable depth_description   String  
attribute depth_description long_name String Depth_description
attribute depth_description units String unitless
variable depth   int  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth actual_range int 10, 5200
attribute depth axis String Z
attribute depth ioos_category String Location
attribute depth long_name String Depth_actual
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable insitu_temp   float  
attribute insitu_temp actual_range float 1.5, 26.6
attribute insitu_temp long_name String Insitu_temp
attribute insitu_temp units String degrees Celsius
variable sample_type   String  
attribute sample_type long_name String Sample_type
attribute sample_type units String unitless
variable incubation_type   String  
attribute incubation_type long_name String Incubation_type
attribute incubation_type units String unitless
variable incubation_pressure   float  
attribute incubation_pressure actual_range float 0.1, 52.0
attribute incubation_pressure long_name String Incubation_pressure
attribute incubation_pressure units String MPa
variable incubation_temp   int  
attribute incubation_temp actual_range int 4, 52
attribute incubation_temp long_name String Incubation_temp
attribute incubation_temp units String degrees Celsius
variable unamended_amended   String  
attribute unamended_amended long_name String Unamended_amended
attribute unamended_amended units String unitless
variable substrate   String  
attribute substrate long_name String Substrate
attribute substrate units String unitless
variable replicate   int  
attribute replicate actual_range int 1, 4
attribute replicate long_name String Replicate
attribute replicate units String unitless
variable timepoint   int  
attribute timepoint actual_range int 0, 2
attribute timepoint long_name String Timepoint
attribute timepoint units String unitless
variable timepoint_days   int  
attribute timepoint_days actual_range int 0, 12
attribute timepoint_days long_name String Timepoint_days
attribute timepoint_days units String days
variable fields_of_view   int  
attribute fields_of_view actual_range int 1, 99
attribute fields_of_view long_name String Fields_of_view
attribute fields_of_view units String unitless
variable SB_Abundance   float  
attribute SB_Abundance actual_range float 0.0, 112000.0
attribute SB_Abundance long_name String Sb_abundance
attribute SB_Abundance units String Cells mL-1
variable Total_Abundance   float  
attribute Total_Abundance actual_range float 4980.0, 3020000.0
attribute Total_Abundance long_name String Total_abundance
attribute Total_Abundance units String Cells mL-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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