ERDDAP > info > bcodmo_dataset_963407_v1

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[AE2413 Bacterial productivity] - Bacterial productivity of samples from three stations in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413, during May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean)		F   I   M	background			BCO-DMO	bcodmo_dataset_963407_v1

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.963407.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-60.0479
attribute	NC_GLOBAL	geospatial_lat_max	double	42.17828
attribute	NC_GLOBAL	geospatial_lat_min	double	34.98703
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-60.0479
attribute	NC_GLOBAL	geospatial_lon_min	double	-73.03333
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	5200.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	30.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://osprey.bco-dmo.org/dataset/963407
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	license	String	The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.
attribute	NC_GLOBAL	Northernmost_Northing	double	42.17828
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	34.98703
attribute	NC_GLOBAL	summary	String	Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).   Through our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.      Here we present the measurement of 3H-leucine incorporation by heterotrophic bacteria using a cold trichloroacetic acid (TCA) and microcentrifuge extraction method (Kirchman, 2001) at different sites in the Western North Atlantic aboard R/V Atlantic Explorer during during the research cruise AE2413 (2024-05-09 to 2024-05-28).  All work and incubations were performed in a UNOLS isotope lab, or within designated areas at the University of North Carolina at Chapel HIll post cruise. This dataset contains collection metadata, environmental conditions, sample types and treatments, incubation conditions, substrate types, radioactivity measurements, and calculated incorporation rates of 3H-leucine.
attribute	NC_GLOBAL	time_coverage_end	String	2024-05-19T15:08:30Z
attribute	NC_GLOBAL	time_coverage_start	String	2024-05-10T22:28:00Z
attribute	NC_GLOBAL	title	String	[AE2413 Bacterial productivity] - Bacterial productivity of samples from three stations in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413, during May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean)
attribute	NC_GLOBAL	Westernmost_Easting	double	-73.03333
variable	deployment		String
attribute	deployment	long_name	String	Deployment
attribute	deployment	units	String	unitless
variable	station		int
attribute	station	actual_range	int	24, 26
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	latitude		float
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	actual_range	float	34.98703, 42.17828
attribute	latitude	axis	String	Y
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude_n
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		float
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	actual_range	float	-73.03333, -60.0479
attribute	longitude	axis	String	X
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude_e
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.71538008E9, 1.71613131E9
attribute	time	axis	String	T
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	Iso_datetime_utc
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	date		String
attribute	date	long_name	String	Date
attribute	date	units	String	unitless
variable	time_local_est		String
attribute	time_local_est	long_name	String	Time_local_est
attribute	time_local_est	units	String	unitless
variable	cast_number		int
attribute	cast_number	actual_range	int	1, 9
attribute	cast_number	long_name	String	Cast_number
attribute	cast_number	units	String	unitless
variable	depth_description		String
attribute	depth_description	long_name	String	Depth_description
attribute	depth_description	units	String	unitless
variable	depth		int
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	actual_range	int	30, 5200
attribute	depth	axis	String	Z
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth_actual
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	insitu_temp		float
attribute	insitu_temp	actual_range	float	2.18, 21.8
attribute	insitu_temp	long_name	String	Insitu_temp
attribute	insitu_temp	units	String	degrees Celsius
variable	sample_type		String
attribute	sample_type	long_name	String	Sample_type
attribute	sample_type	units	String	unitless
variable	incubation_type		String
attribute	incubation_type	long_name	String	Incubation_type
attribute	incubation_type	units	String	unitless
variable	incubation_pressure		float
attribute	incubation_pressure	actual_range	float	0.1, 52.0
attribute	incubation_pressure	long_name	String	Incubation_pressure
attribute	incubation_pressure	units	String	MPa
variable	incubation_temp		int
attribute	incubation_temp	actual_range	int	4, 22
attribute	incubation_temp	long_name	String	Incubation_temp
attribute	incubation_temp	units	String	degrees Celsius
variable	unamended_amended		String
attribute	unamended_amended	long_name	String	Unamended_amended
attribute	unamended_amended	units	String	unitless
variable	substrate		String
attribute	substrate	long_name	String	Substrate
attribute	substrate	units	String	unitless
variable	incubation_time		float
attribute	incubation_time	actual_range	float	5.0, 50.5
attribute	incubation_time	long_name	String	Incubation_time
attribute	incubation_time	units	String	hours
variable	DPM_Kill		int
attribute	DPM_Kill	actual_range	int	18, 39
attribute	DPM_Kill	long_name	String	Dpm_kill
attribute	DPM_Kill	units	String	disintegrations per minute (dpm)
variable	DPM_rep1		int
attribute	DPM_rep1	actual_range	int	19, 1021
attribute	DPM_rep1	long_name	String	Dpm_rep1
attribute	DPM_rep1	units	String	disintegrations per minute (dpm)
variable	DPM_rep2		int
attribute	DPM_rep2	actual_range	int	16, 1007
attribute	DPM_rep2	long_name	String	Dpm_rep2
attribute	DPM_rep2	units	String	disintegrations per minute (dpm)
variable	DPM_rep3		int
attribute	DPM_rep3	actual_range	int	18, 1030
attribute	DPM_rep3	long_name	String	Dpm_rep3
attribute	DPM_rep3	units	String	disintegrations per minute (dpm)
variable	Average_incorp		int
attribute	Average_incorp	actual_range	int	-6, 457
attribute	Average_incorp	long_name	String	Average_incorp
attribute	Average_incorp	units	String	pmol L-1
variable	H3_Leu		float
attribute	H3_Leu	actual_range	float	-0.2, 60.2
attribute	H3_Leu	long_name	String	H3_leu
attribute	H3_Leu	units	String	pmol L-1 h-1
variable	stdev		float
attribute	stdev	actual_range	float	0.0, 15.6
attribute	stdev	long_name	String	Stdev
attribute	stdev	units	String	pmol L-1 h-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.