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     data   graph     files  public [EN638 cell count selfish] - Detection and Quantification of Cells Exhibiting 'Selfish'
Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May
2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved
organic carbon degradation)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.988179.1
attribute NC_GLOBAL Easternmost_Easting double -53.3949
attribute NC_GLOBAL geospatial_lat_max double 42.83954
attribute NC_GLOBAL geospatial_lat_min double 34.6369
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -53.3949
attribute NC_GLOBAL geospatial_lon_min double -72.0021
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 5580.0
attribute NC_GLOBAL geospatial_vertical_min double 2.5
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://osprey.bco-dmo.org/dataset/988179 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL Northernmost_Northing double 42.83954
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 34.6369
attribute NC_GLOBAL summary String Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic carbon (POC), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood.
 
A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed.
 
Here we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting "selfish" uptake, and total cellular abundance.
attribute NC_GLOBAL time_coverage_end String 2019-05-25T07:30:00Z
attribute NC_GLOBAL time_coverage_start String 2019-05-17T00:20:00Z
attribute NC_GLOBAL title String [EN638 cell count selfish] - Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation)
attribute NC_GLOBAL Westernmost_Easting double -72.0021
variable deployment   String  
attribute deployment long_name String Deployment
attribute deployment units String unitless
variable station   String  
attribute station long_name String Station
attribute station units String unitless
variable latitude   float  
attribute latitude _CoordinateAxisType String Lat
attribute latitude actual_range float 34.6369, 42.83954
attribute latitude axis String Y
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   float  
attribute longitude _CoordinateAxisType String Lon
attribute longitude actual_range float -72.0021, -53.3949
attribute longitude axis String X
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.5580524E9, 1.5587694E9
attribute time axis String T
attribute time ioos_category String Time
attribute time long_name String Iso_datetime_utc
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z
variable cast_number   String  
attribute cast_number long_name String Cast_number
attribute cast_number units String unitless
variable depth_name   String  
attribute depth_name long_name String Depth_name
attribute depth_name units String unitless
variable depth   float  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth actual_range float 2.5, 5580.0
attribute depth axis String Z
attribute depth ioos_category String Location
attribute depth long_name String Depth_actual
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable sample_type   String  
attribute sample_type long_name String Sample_type
attribute sample_type units String unitless
variable unamended_amended   String  
attribute unamended_amended long_name String Unamended_amended
attribute unamended_amended units String unitless
variable substrate   String  
attribute substrate long_name String Substrate
attribute substrate units String unitless
variable timepoint_days   float  
attribute timepoint_days actual_range float 0.0, 30.0
attribute timepoint_days long_name String Timepoint_days
attribute timepoint_days units String days
variable timepoint_hours   float  
attribute timepoint_hours actual_range float 1.0, 720.0
attribute timepoint_hours long_name String Timepoint_hours
attribute timepoint_hours units String hours
variable replicate_1_selfish_percent   float  
attribute replicate_1_selfish_percent actual_range float -14.5, 20.6
attribute replicate_1_selfish_percent long_name String Replicate_1_selfish_percent
attribute replicate_1_selfish_percent units String percent
variable replicate_2_selfish_percent   float  
attribute replicate_2_selfish_percent actual_range float -9.0, 30.2
attribute replicate_2_selfish_percent long_name String Replicate_2_selfish_percent
attribute replicate_2_selfish_percent units String percent
variable replicate_3_selfish_percent   float  
attribute replicate_3_selfish_percent actual_range float -7.2, 57.2
attribute replicate_3_selfish_percent long_name String Replicate_3_selfish_percent
attribute replicate_3_selfish_percent units String percent
variable average_selfish_percent   float  
attribute average_selfish_percent actual_range float -9.4, 32.8
attribute average_selfish_percent long_name String Average_selfish_percent
attribute average_selfish_percent units String percent
variable standard_deviation_selfish_percent   float  
attribute standard_deviation_selfish_percent actual_range float 0.0, 21.2
attribute standard_deviation_selfish_percent long_name String Standard_deviation_selfish_percent
attribute standard_deviation_selfish_percent units String percent
variable replicate_1_substratecells   float  
attribute replicate_1_substratecells actual_range float -35176.0, 198000.0
attribute replicate_1_substratecells long_name String Replicate_1_substratecells
attribute replicate_1_substratecells units String Cells/ml
variable replicate_2_substratecells   float  
attribute replicate_2_substratecells actual_range float -36944.0, 327666.0
attribute replicate_2_substratecells long_name String Replicate_2_substratecells
attribute replicate_2_substratecells units String Cells/ml
variable replicate_3_substratecells   float  
attribute replicate_3_substratecells actual_range float -35047.0, 353000.0
attribute replicate_3_substratecells long_name String Replicate_3_substratecells
attribute replicate_3_substratecells units String Cells/ml
variable average_substratecells   float  
attribute average_substratecells actual_range float -35024.0, 318359.0
attribute average_substratecells long_name String Average_substratecells
attribute average_substratecells units String Cells/ml
variable standard_deviation_substratecells   float  
attribute standard_deviation_substratecells actual_range float 0.0, 173772.2
attribute standard_deviation_substratecells long_name String Standard_deviation_substratecells
attribute standard_deviation_substratecells units String Cells/ml
variable replicate_1_cells   float  
attribute replicate_1_cells actual_range float 11100.0, 3370000.0
attribute replicate_1_cells long_name String Replicate_1_cells
attribute replicate_1_cells units String Cells/ml
variable replicate_2_cells   float  
attribute replicate_2_cells actual_range float 0.0, 2570000.0
attribute replicate_2_cells long_name String Replicate_2_cells
attribute replicate_2_cells units String Cells/ml
variable replicate_3_cells   float  
attribute replicate_3_cells actual_range float 0.0, 2880000.0
attribute replicate_3_cells long_name String Replicate_3_cells
attribute replicate_3_cells units String Cells/ml
variable average_cells   float  
attribute average_cells actual_range float 11767.0, 2663333.0
attribute average_cells long_name String Average_cells
attribute average_cells units String Cells/ml
variable standard_deviation_cells   float  
attribute standard_deviation_cells actual_range float 0.0, 945797.7
attribute standard_deviation_cells long_name String Standard_deviation_cells
attribute standard_deviation_cells units String Cells/ml

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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