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Dataset Title: | [Coral-bacterioplankton data from mesocosm experiments] - Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013 (Fundamental Coral-Microbial Associations) |
Institution: | BCO-DMO (Dataset ID: bcodmo_dataset_652849) |
Information: | Summary | License | ISO 19115 | Metadata | Background | Data Access Form | Files |
Attributes { s { treatment { String bcodmo_name "treatment"; String description "mesocosm treatment; control; mucus addition; and coral with artifical lights"; String long_name "Treatment"; String units "unitless"; } treatment_description { String bcodmo_name "treatment"; String description "mesocosm condition; coral: presence or absence of colonies; mucus addition; or control"; String long_name "Treatment Description"; String units "unitless"; } tank { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 9; String bcodmo_name "tank"; String description "tank number"; String long_name "Tank"; String units "unitless"; } mesocosm { String bcodmo_name "treatment"; String description "treatment and tank number"; String long_name "Mesocosm"; String units "unitless"; } timepoint_name { String bcodmo_name "days"; String description "day during duration of 12 day experiment"; String long_name "Timepoint Name"; String units "days"; } timepoint_days { Float32 _FillValue NaN; Float32 actual_range 0.0, 11.99; String bcodmo_name "days"; String description "timepoint within 12 day experiment"; String long_name "Timepoint Days"; String units "days"; } temp { Float32 _FillValue NaN; Float32 actual_range 24.5, 26.9; String bcodmo_name "temperature"; String description "temperature in mesocosm at each timepoint"; String long_name "Temperature"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/"; String units "celsius"; } salinity { Float32 _FillValue NaN; Float32 actual_range 36.32, 38.6; String bcodmo_name "sal"; Float64 colorBarMaximum 37.0; Float64 colorBarMinimum 32.0; String description "salinity in mesocosm at each timepoint"; String long_name "Sea Water Practical Salinity"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/"; String units "ppt"; } DAPI_count { Float32 _FillValue NaN; Float32 actual_range 359000.0, 2030000.0; String bcodmo_name "cell_concentration"; Float64 colorBarMaximum 100.0; Float64 colorBarMinimum 0.0; String description "0 6-diamidino-2-phenyl dihydrochloride (DAPI) counts of picoplankton"; String long_name "DAPI Count"; String units "cells per milliliter"; } accession_number { String bcodmo_name "accession number"; String description "SSU rRNA gene V4 region sequences; NCBI SRA Accession"; String long_name "Accession Number"; String units "unitless"; } nonPigmentPico { Float32 _FillValue NaN; Float32 actual_range 204000.0, 7020000.0; String bcodmo_name "cell_concentration"; String description "Non-pigmented picoplankton cells counted by flow cytometry"; String long_name "Non Pigment Pico"; String units "cells per milliliter"; } synechococcus { Int32 _FillValue 2147483647; Int32 actual_range 39, 108386; String bcodmo_name "cell_concentration"; String description "Synechococcus cells counted by flow cytometry"; String long_name "Synechococcus"; String units "cells per milliliter"; } picoeukaryote { Int16 _FillValue 32767; Int16 actual_range 473, 9883; String bcodmo_name "cell_concentration"; String description "picoeukaryote cells counted by flow cytometry"; String long_name "Picoeukaryote"; String units "cells per milliliter"; } lowHeterotrophicBac { Float32 _FillValue NaN; Float32 actual_range 8320.0, 1780000.0; String bcodmo_name "cell_concentration"; String description "Low DNA heterotropic bacteria counted by flow cytometry"; String long_name "Low Heterotrophic Bac"; String units "cells per milliliter"; } highHeterotrophicBac { Float32 _FillValue NaN; Float32 actual_range 196000.0, 6550000.0; String bcodmo_name "cell_concentration"; String description "High DNA concent heterotrophic bacteria counted by flow cytometry"; String long_name "High Heterotrophic Bac"; String units "cells per milliliter"; } SAR11 { Float32 _FillValue NaN; Float32 actual_range 8310.0, 515000.0; String bcodmo_name "cell_concentration"; String description "SAR11 cell counts by fluorescence in situ hybridization (FISH)"; String long_name "SAR11"; String units "cells per milliliter"; } rhodobacteracea { Float32 _FillValue NaN; Float32 actual_range 8670.0, 324000.0; String bcodmo_name "cell_concentration"; String description "Rhodobacteracea cell counts by fluorescence in situ hybridization (FISH)"; String long_name "Rhodobacteracea"; String units "cells per milliliter"; } alteromonas { Float32 _FillValue NaN; Float32 actual_range 0.0, 98300.0; String bcodmo_name "cell_concentration"; String description "Alteromonas cell counts by fluorescence in situ hybridization (FISH)"; String long_name "Alteromonas"; String units "cells per milliliter"; } vibrio { Float32 _FillValue NaN; Float32 actual_range 0.0, 55400.0; String bcodmo_name "cell_concentration"; String description "Vibrio cell counts by fluorescence in situ hybridization (FISH)"; String long_name "Vibrio"; String units "cells per milliliter"; } thaumarchaeota { Float32 _FillValue NaN; Float32 actual_range 0.0, 80500.0; String bcodmo_name "cell_concentration"; String description "Thaumarchaeota cell counts by fluorescence in situ hybridization (FISH)"; String long_name "Thaumarchaeota"; String units "cells per milliliter"; } euryarchaeota { Float32 _FillValue NaN; Float32 actual_range 0.0, 149000.0; String bcodmo_name "cell_concentration"; String description "Euryarchaeota cell counts by fluorescence in situ hybridization (FISH)"; String long_name "Euryarchaeota"; String units "cells per milliliter"; } P04 { Float32 _FillValue NaN; Float32 actual_range 0.03, 0.2; String bcodmo_name "PO4"; String description "phosphate in seawater samples"; String long_name "P04"; String units "micromoles"; } N03_N02 { Float32 _FillValue NaN; Float32 actual_range 0.52, 10.22; String bcodmo_name "NO3_NO2"; String description "nitrate + nitrate in seawater samples"; String long_name "N03 N02"; String units "micromoles"; } Si04 { Float32 _FillValue NaN; Float32 actual_range 1.1, 5.4; String bcodmo_name "SiO4"; String description "silicate in seawater samples"; String long_name "Si04"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/"; String units "micromoles"; } N02 { Float32 _FillValue NaN; Float32 actual_range 0.06, 0.48; String bcodmo_name "NO2"; String description "nitrite in seawater samples"; String long_name "N02"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/"; String units "micromoles"; } NH4 { Float32 _FillValue NaN; Float32 actual_range 0.3, 7.09; String bcodmo_name "Ammonium"; Float64 colorBarMaximum 5.0; Float64 colorBarMinimum 0.0; String description "ammonium in seawater samples"; String long_name "Mole Concentration Of Ammonium In Sea Water"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/"; String units "micromoles"; } DOC { Float32 _FillValue NaN; Float32 actual_range 71.82, 110.86; String bcodmo_name "DOC"; String description "dissolved organic carbon in seawater samples"; String long_name "DOC"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/"; String units "micromoles"; } bacGrowth_constant_mean { Float32 _FillValue NaN; Float32 actual_range -0.31, 0.5; String bcodmo_name "mean"; String description "k; mean bacterial growth constant"; String long_name "Bac Growth Constant Mean"; String units "days -1"; } bacGrazing_coefficient_mean { Float32 _FillValue NaN; Float32 actual_range -0.32, 0.56; String bcodmo_name "mean"; String description "g; mean bact grazing coefficient"; String long_name "Bac Grazing Coefficient Mean"; String units "days -1"; } synechococcusGrowth_constant_mean { Float32 _FillValue NaN; Float32 actual_range -1.18, 0.03; String bcodmo_name "mean"; String description "k; mean synechococcus growth constant"; String long_name "Synechococcus Growth Constant Mean"; String units "days -1"; } synechococcusGrazing_coefficient { Float32 _FillValue NaN; Float32 actual_range -1.21, -0.55; String bcodmo_name "unknown"; String description "synechococcus grazing coefficient"; String long_name "Synechococcus Grazing Coefficient"; String units "days -1"; } SAR11Growth_constant_mean { Float32 _FillValue NaN; Float32 actual_range -0.91, 1.19; String bcodmo_name "mean"; String description "k; mean SAR11 growth constant"; String long_name "SAR11 Growth Constant Mean"; String units "days -1"; } SAR11Grazing_coefficient { Float32 _FillValue NaN; Float32 actual_range -0.98, -0.26; String bcodmo_name "unknown"; String description "SAR11 grazing coefficient"; String long_name "SAR11 Grazing Coefficient"; String units "days -1"; } rhodobacteraceaGrowth_constant_mean { Float32 _FillValue NaN; Float32 actual_range -0.89, 1.34; String bcodmo_name "mean"; String description "k; mean Rhodobacteracea growth constant"; String long_name "Rhodobacteracea Growth Constant Mean"; String units "days -1"; } rhodobacteraceaGrazing_coefficient { Float32 _FillValue NaN; Float32 actual_range -0.92, 0.06; String bcodmo_name "unknown"; String description "Rhodobacteracea grazing coefficient"; String long_name "Rhodobacteracea Grazing Coefficient"; String units "days -1"; } } NC_GLOBAL { String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv"; String acquisition_description "Colonies of Porites astreoides corals (5-20 cm diameter, 30-90 g wet weight) were removed from 3-9 m depth on the Bermudian reefs Hog Breaker (N 32 27.5\\u2019 W 64 49.8\\u2019), an unnamed reef (N 32 26.042\\u2019 W 64 49.248\\u2019), and Three Hills Shoal (N 32 41\\u2019 W 64 73.3\\u2019) in July 2013. Ten colonies were taken from each site in compliance with the Bermuda Institute of Ocean Sciences (BIOS) Collection and Experimental Ethics Policy and were considered Limited Impact Research and as such a collection permit was not required. The colonies were immediately placed in collection bags at depth, sealed and transported in a large collection cooler (greater than 1 h) to the laboratory.\\u00a0 At BIOS, the colonies were allowed to acclimate to a mesocosm housing and held in aerated, outdoor fiberglass mesocosms with a flow-through seawater system fed with reef water for two (Three Hills Shoal Reef colonies) or 15 days (all other colonies) prior to the start of the experimental period.\\u00a0 For the experiment, the mesocosms consisted of nine, 30 L static aquaria that were set up as previously described (de Putron et al. 2010). Inshore water from 20 meters off shore and one meter in depth was used to fill the aquaria and was pumped through a BIOS flow-through sample system that consisted of a coarse mesh filter and holding tanks followed by a step filtration system of 50 um, then 5 um, to remove larger organisms including some planktonic grazers. Mesocosms were then left static for the 12-day experiment. Mesocosms were randomly arranged to consist of three control, three mucus addition, and three coral mesocosms, with artificial lights providing 61 mol quanta m-2 s-1 photosynthetically active radiation during sunlight hours consistent with the low end of known compensation ranges (3-233 mol quanta m-2 s-1 according to Mass and colleagues (2007) to minimize bleaching. Mesocosms were covered with a transparent plastic film cover and aerated with an air wand bubbler (de Putron et al. 2010). Water was collected from the mesocosms via a syringe- siphon system using silicon tubing.\\u00a0 Inline, combusted GF/F filters (Whatman, Maidstone, UK) were attached for samples intended for dissolved organic carbon (DOC) analysis. The reef water temperature, salinity, and conductivity in all mesocosms was monitored daily throughout the experiment using a YSI Professional Plus probe (Yellow Springs, Ohio).\\u00a0 Over the course of 12 days, the mesocosms were subjected to one of three conditions: coral (presence/absence of colonies), mucus additions or control (no additions).\\u00a0 For the coral mesocosms, the experimental corals (four colonies per mesocosm, three mesocosms) were placed within the mesocosms on days 0 through 4 and removed after water sampling on day 4. Corals were removed for 48-hour until after the sampling procedure on day 6, where they were held in running seawater similarly filtered as in the mesocosms, and reintroduced back into their respective mesocosms from days 6 to 10 and removed again after the sampling procedure on day 10. P. astreoides colonies were not fed over the course of the 12-day experimental period. For the mucus addition mesocosms, mucus was extracted from P. astreoides colonies (n = 18) that were not utilized in the experiment and were maintained in a separate, flow-through seawater tank.\\u00a0 These corals (n=18) were taken into the lab and inverted on a funnel to collect mucus for 2 h, a timeframe determined from a preliminary experiment, and the corals returned to the holding aquaria with flow through seawater. Mucus from the additional colonies was pooled and 5 ml was added to each mucus addition mesocosm on days 0.2, 2, 6 and 8.\\u00a0 Mucus from the experimental corals (n = 12; named corals A-L from coral tanks 4, 5 and 6) was sampled for community composition prior to day 0, as well as at the end of day 4 and at the end of day 10 of the experiment.\\u00a0 For the latter two timepoints, the corals were removed from the experimental mesocosms for 48 hours.\\u00a0 Seawater samples (50 ml) for cell counts and fluorescence in situ hybridization (FISH) analyses were taken daily from all mesocosms, fixed with formalin to a final concentration of 10% in the dark for 20 min, and stored at -80C.\\u00a0 Samples were thawed and 3-5 ml were filtered onto Irgalan Black stained 25 mm, 0.2 um polycarbonate filters (Nucleopore, Whatman) under gentle vacuum (100 mmHg) and stained with 1 ml of 0, 6-diamidino-2-phenyl dihydrochloride (5 ug ml-1, DAPI, SIGMA-Aldrich, St. Louis, MO) (Porter and Feig 1980). The filters were mounted onto slides with Resolve immersion oil (high viscosity) (Resolve, Richard-Allan Scientific, Kalamazoo, MI) and stored at \\u201320 degrees celsius. Slides were then enumerated using an AX70 epifluorescent microscope (Olympus, Tokyo, Japan) under ultraviolet excitation at 100x magnification. At least 500 cells (10 fields) were counted for picoplankton abundance.\\u00a0\\u00a0 \\u00a0 FISH was utilized to quantify the abundance of the major picoplankton phylotypes present in the seawater and mucus, and was conducted using previously published protocols and Cy3 labeled probes (Parsons et al. 2014). The bacterial and archaeal groups quantified included the SAR11 clade (152R, 441R, 542R, 732R probes), Alteromonas spp. (AC137R), Vibrio spp. (127R), Rhodobacteracea (536R), Euryarchaeota (Eury806) and Thaumarchaeota (Cren537). Fixed seawater samples (3\\u20135 ml) were filtered onto 25 mm, 0.2 um polycarbonate filters and stored at \\u221220 degrees celcius with desiccant. Quarter filters were washed in 95% ethanol and then probed according to previous protocols (Morris et al. 2002; Parsons et al. 2011, 2014). The cell abundances of the picoplankton phylotypes mentioned above were then determined using image analysis (Parsons et al. 2011, 2014). Detection of Cy3-positive cells and their ratio to DAPI-positive cells was aided by image analysis using an Olympus AX70 microscope (Olympus, Japan) equipped with a Toshiba 3CCD video camera (IK-TU40A Toshiba, Japan), a computer assisted frame grabber and appropriate dichroic filters (Morris et al. 2002; Carlson et al. 2010). Brief exposure times of 1 and 5 seconds were used for DAPI and Cy3 image channels, respectively.\\u00a0 Cy3 images were segmented with Image Pro Plus software (Media Cybernetics, Bethesda, MD) and overlaid onto corresponding segmented DAPI images (Parsons et al. 2014). Objects with overlapping signals in both Cy3 and DAPI images were counted as probe positive. The negative control was determined similarly and subtracted from the positive probe counts to correct for autofluorescence and non-specific binding.\\u00a0 (For Grazing Rates Processing, see below) Seawater picoplankton biomass for nucleic acids was taken from all experimental mesocosms on days 0, 2, 4, 6, 8, 10 and 12 of the experiment, and from coral mucus extracted from the experimental corals on days 2, 4, and 10 and processing followed a method modified from Giovannoni and colleagues (1990, 1996). 500 ml of water or 1 ml of coral mucus was filtered through a 47 mm, 0.2 um pore filter under gentle vacuum (100 mm Hg), placed into a 4 ml cryovial and stored in 1 ml of sterile sucrose lysis buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris.HCl) at -80 degrees celsius. For DNA extractions of the tissue, mucus and coral samples, sodium dodecyl sulfate to 1% and proteinase K to 200 ug ml-1 were added to the sample and incubated at 37 degrees celsius for 30 min and then at 55 degrees celsius for 30 min. The lysates were extracted with an equal volume of phenol:isoamylalcohol:chloroform (25:1:24) followed by two subsequent equal volumes of isoamylalcohol:chloroform (1:24). The DNA was purified by precipitation using sodium acetate (3M) and isopropanol (100%) for at least 1 hour at -20oC and centrifuged at room temperature for 30 min at 20,000 x g. The resulting pellet was washed with 80% ethanol, vortexed for 30 s and centrifuged at 16,000 x g for 10 min. The pellet was dried and stored at -20C.\\u00a0 (See below for Microbial Processing description.) Seawater samples were analyzed for macronutrients (nitrate + nitrite, nitrite, ammonium, ortho phosphate, and silicic acid)\\u00a0 at Oregon State University using a continuous segmented flow system consisting of a Technicon AutoAnalyzer II (SEAL Analytical) and an Alpkem RFA 300 Rapid Flow Analyzer (Alpkem) as conducted previously (Apprill and Rapp\\u00e9 2011). DOC was determined via high temperature combustion on a modified Shimadzu TOC-V (Shimadzu Scientific Instruments, Columbia, MD) (Carlson et al. 2010). Flow cytometry was performed on 1 ml seawater preserved to a final concentration of 4% paraformaldehyde to enumerate pigmented picoeukaryotes, Synechococcus, and non-pigmented picoplankton using methods described in Apprill and Rapp\\u00e9 (2011). High and low DNA-containing cells were enumerated following SybrGreen staining. The ANOVA and Tukey\\u2019s HSD statistics were conducted as described below."; String awards_0_award_nid "564441"; String awards_0_award_number "OCE-1233612"; String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233612"; String awards_0_funder_name "NSF Division of Ocean Sciences"; String awards_0_funding_acronym "NSF OCE"; String awards_0_funding_source_nid "355"; String awards_0_program_manager "David L. Garrison"; String awards_0_program_manager_nid "50534"; String cdm_data_type "Other"; String comment "Bacterioplankton data from aquaria experiments A. Apprill, PI Version 29 July 2016 Columns Measured with Flow Cytometry: \t nonPigmentPico,synechococcus,picoeukaryote, \t lowHeterotrophicBac, and highHeterotrophicBac Columns Measured with FISH: \t SAR11,rhodobacteracea,alteromonas,vibrio, \t thaumarchaeota, and euryarchaeota Column Units: \t Temperature: Celsius \t Salinity: PPT \t Flow Cytometry and FISH: cells per mL \t DAPI counts: cells per mL \t Growth and Grazing: day -1 \t Nutrients: micromoles"; String Conventions "COARDS, CF-1.6, ACDD-1.3"; String creator_email "info@bco-dmo.org"; String creator_name "BCO-DMO"; String creator_type "institution"; String creator_url "https://www.bco-dmo.org/"; String data_source "extract_data_as_tsv version 2.3 19 Dec 2019"; String date_created "2016-07-29T13:07:47Z"; String date_modified "2019-06-05T18:00:55Z"; String defaultDataQuery "&time<now"; String doi "10.1575/1912/bco-dmo.652849.1"; String history "2024-10-12T16:06:15Z (local files) 2024-10-12T16:06:15Z https://erddap.bco-dmo.org/tabledap/bcodmo_dataset_652849.das"; String infoUrl "https://www.bco-dmo.org/dataset/652849"; String institution "BCO-DMO"; String instruments_0_dataset_instrument_description "Slides were enumerated using this microscope."; String instruments_0_dataset_instrument_nid "652874"; String instruments_0_description "Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments."; String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB06/"; String instruments_0_instrument_name "Microscope-Fluorescence"; String instruments_0_instrument_nid "695"; String instruments_0_supplied_name "AX70 epifluorescent microscope"; String instruments_1_acronym "Thermal Cycler"; String instruments_1_dataset_instrument_description "Reactions carried out on a Bio-Rad thermocycler."; String instruments_1_dataset_instrument_nid "652875"; String instruments_1_description "General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. (adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)"; String instruments_1_instrument_name "PCR Thermal Cycler"; String instruments_1_instrument_nid "471582"; String instruments_1_supplied_name "Bio-Rad thermocycler"; String keywords "accession, accession_number, alteromonas, ammonia, ammonium, bac, bacGrazing_coefficient_mean, bacGrowth_constant_mean, bco, bco-dmo, biological, chemical, chemistry, coefficient, commerce, concentration, constant, count, dapi, DAPI_count, data, dataset, days, density, department, description, dmo, doc, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Salinity/Density > Salinity, erddap, euryarchaeota, grazing, growth, heterotrophic, high, highHeterotrophicBac, low, lowHeterotrophicBac, management, mean, mesocosm, mole, mole_concentration_of_ammonium_in_sea_water, n02, n03, N03_N02, name, nh4, non, nonPigmentPico, number, ocean, oceanography, oceans, office, p04, pico, picoeukaryote, pigment, practical, preliminary, rhodobacteracea, rhodobacteraceaGrazing_coefficient, rhodobacteraceaGrowth_constant_mean, salinity, sar11, SAR11Grazing_coefficient, SAR11Growth_constant_mean, science, sea, sea_water_practical_salinity, seawater, si04, synechococcus, synechococcusGrazing_coefficient, synechococcusGrowth_constant_mean, tank, temperature, thaumarchaeota, timepoint, timepoint_days, timepoint_name, treatment, treatment_description, vibrio, water"; String keywords_vocabulary "GCMD Science Keywords"; String license "https://www.bco-dmo.org/dataset/652849/license"; String metadata_source "https://www.bco-dmo.org/api/dataset/652849"; String param_mapping "{'652849': {}}"; String parameter_source "https://www.bco-dmo.org/mapserver/dataset/652849/parameters"; String people_0_affiliation "Woods Hole Oceanographic Institution"; String people_0_affiliation_acronym "WHOI"; String people_0_person_name "Amy Apprill"; String people_0_person_nid "553489"; String people_0_role "Principal Investigator"; String people_0_role_type "originator"; String people_1_affiliation "Woods Hole Oceanographic Institution"; String people_1_affiliation_acronym "WHOI BCO-DMO"; String people_1_person_name "Hannah Ake"; String people_1_person_nid "650173"; String people_1_role "BCO-DMO Data Manager"; String people_1_role_type "related"; String project "Coral Microbial Relationships"; String projects_0_acronym "Coral Microbial Relationships"; String projects_0_description "Description from NSF award abstract: Reef-building corals are in decline worldwide due in part to climate change and other human activities, and it is becoming increasingly important to understand what aspects of coral biology are degraded by environmental stress which then leads to coral mortality. It is now widely known that corals harbor communities of bacteria and archaea that are believed to play important roles in maintaining the health of their hosts, but we lack any appreciable understanding about the identity of the microbial associates regularly residing within healthy, reef-building corals. This project asks the central question: do reef-building corals harbor fundamental or persistent microbial associates that are symbiotic within their tissues? In order to address this hypothesis, the investigator will assess the identity of the bacterial and archaeal microbes using a variety of molecular and microscopy approaches that includes the identification and localization of a widespread group of coral bacterial associates belonging to the genus Endozoicomonas. The results of this study will then be used to develop additional questions about the role of these microbial associates in nutrient cycling and how they contribute to the health and survival of corals."; String projects_0_end_date "2016-08"; String projects_0_geolocation "Florida Keys, Federated States of Micronesia, Red Sea, & Bermuda"; String projects_0_name "Fundamental Coral-Microbial Associations"; String projects_0_project_nid "564442"; String projects_0_start_date "2012-09"; String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)"; String publisher_type "institution"; String sourceUrl "(local files)"; String standard_name_vocabulary "CF Standard Name Table v55"; String summary "Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013"; String title "[Coral-bacterioplankton data from mesocosm experiments] - Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013 (Fundamental Coral-Microbial Associations)"; String version "1"; String xml_source "osprey2erddap.update_xml() v1.3"; } }
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Tabledap request URLs must be in the form
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For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
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