ERDDAP > info > bcodmo_dataset_652849

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013		I   M	background			BCO-DMO	bcodmo_dataset_652849

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Colonies of Porites astreoides corals (5-20 cm diameter, 30-90 g wet weight) were removed from 3-9 m depth on the Bermudian reefs Hog Breaker (N 32 27.5\u2019 W 64 49.8\u2019), an unnamed reef (N 32 26.042\u2019 W 64 49.248\u2019), and Three Hills Shoal (N 32 41\u2019 W 64 73.3\u2019) in July 2013. Ten colonies were taken from each site in compliance with the Bermuda Institute of Ocean Sciences (BIOS) Collection and Experimental Ethics Policy and were considered Limited Impact Research and as such a collection permit was not required. The colonies were immediately placed in collection bags at depth, sealed and transported in a large collection cooler (greater than 1 h) to the laboratory.\u00a0 At BIOS, the colonies were allowed to acclimate to a mesocosm housing and held in aerated, outdoor fiberglass mesocosms with a flow-through seawater system fed with reef water for two (Three Hills Shoal Reef colonies) or 15 days (all other colonies) prior to the start of the experimental period.\u00a0 For the experiment, the mesocosms consisted of nine, 30 L static aquaria that were set up as previously described (de Putron et al. 2010). Inshore water from 20 meters off shore and one meter in depth was used to fill the aquaria and was pumped through a BIOS flow-through sample system that consisted of a coarse mesh filter and holding tanks followed by a step filtration system of 50 um, then 5 um, to remove larger organisms including some planktonic grazers. Mesocosms were then left static for the 12-day experiment. Mesocosms were randomly arranged to consist of three control, three mucus addition, and three coral mesocosms, with artificial lights providing 61 mol quanta m-2 s-1 photosynthetically active radiation during sunlight hours consistent with the low end of known compensation ranges (3-233 mol quanta m-2 s-1 according to Mass and colleagues (2007) to minimize bleaching. Mesocosms were covered with a transparent plastic film cover and aerated with an air wand bubbler (de Putron et al. 2010). Water was collected from the mesocosms via a syringe- siphon system using silicon tubing.\u00a0 Inline, combusted GF/F filters (Whatman, Maidstone, UK) were attached for samples intended for dissolved organic carbon (DOC) analysis. The reef water temperature, salinity, and conductivity in all mesocosms was monitored daily throughout the experiment using a YSI Professional Plus probe (Yellow Springs, Ohio).\u00a0 Over the course of 12 days, the mesocosms were subjected to one of three conditions: coral (presence/absence of colonies), mucus additions or control (no additions).\u00a0 For the coral mesocosms, the experimental corals (four colonies per mesocosm, three mesocosms) were placed within the mesocosms on days 0 through 4 and removed after water sampling on day 4. Corals were removed for 48-hour until after the sampling procedure on day 6, where they were held in running seawater similarly filtered as in the mesocosms, and reintroduced back into their respective mesocosms from days 6 to 10 and removed again after the sampling procedure on day 10. P. astreoides colonies were not fed over the course of the 12-day experimental period. For the mucus addition mesocosms, mucus was extracted from P. astreoides colonies (n = 18) that were not utilized in the experiment and were maintained in a separate, flow-through seawater tank.\u00a0 These corals (n=18) were taken into the lab and inverted on a funnel to collect mucus for 2 h, a timeframe determined from a preliminary experiment, and the corals returned to the holding aquaria with flow through seawater. Mucus from the additional colonies was pooled and 5 ml was added to each mucus addition mesocosm on days 0.2, 2, 6 and 8.\u00a0 Mucus from the experimental corals (n = 12; named corals A-L from coral tanks 4, 5 and 6) was sampled for community composition prior to day 0, as well as at the end of day 4 and at the end of day 10 of the experiment.\u00a0 For the latter two timepoints, the corals were removed from the experimental mesocosms for 48 hours.\u00a0 Seawater samples (50 ml) for cell counts and fluorescence in situ hybridization (FISH) analyses were taken daily from all mesocosms, fixed with formalin to a final concentration of 10% in the dark for 20 min, and stored at -80C.\u00a0 Samples were thawed and 3-5 ml were filtered onto Irgalan Black stained 25 mm, 0.2 um polycarbonate filters (Nucleopore, Whatman) under gentle vacuum (100 mmHg) and stained with 1 ml of 0, 6-diamidino-2-phenyl dihydrochloride (5 ug ml-1, DAPI, SIGMA-Aldrich, St. Louis, MO) (Porter and Feig 1980). The filters were mounted onto slides with Resolve immersion oil (high viscosity) (Resolve, Richard-Allan Scientific, Kalamazoo, MI) and stored at \u201320 degrees celsius. Slides were then enumerated using an AX70 epifluorescent microscope (Olympus, Tokyo, Japan) under ultraviolet excitation at 100x magnification. At least 500 cells (10 fields) were counted for picoplankton abundance.\u00a0\u00a0 \u00a0 FISH was utilized to quantify the abundance of the major picoplankton phylotypes present in the seawater and mucus, and was conducted using previously published protocols and Cy3 labeled probes (Parsons et al. 2014). The bacterial and archaeal groups quantified included the SAR11 clade (152R, 441R, 542R, 732R probes), Alteromonas spp. (AC137R), Vibrio spp. (127R), Rhodobacteracea (536R), Euryarchaeota (Eury806) and Thaumarchaeota (Cren537). Fixed seawater samples (3\u20135 ml) were filtered onto 25 mm, 0.2 um polycarbonate filters and stored at \u221220 degrees celcius with desiccant. Quarter filters were washed in 95% ethanol and then probed according to previous protocols (Morris et al. 2002; Parsons et al. 2011, 2014). The cell abundances of the picoplankton phylotypes mentioned above were then determined using image analysis (Parsons et al. 2011, 2014). Detection of Cy3-positive cells and their ratio to DAPI-positive cells was aided by image analysis using an Olympus AX70 microscope (Olympus, Japan) equipped with a Toshiba 3CCD video camera (IK-TU40A Toshiba, Japan), a computer assisted frame grabber and appropriate dichroic filters (Morris et al. 2002; Carlson et al. 2010). Brief exposure times of 1 and 5 seconds were used for DAPI and Cy3 image channels, respectively.\u00a0 Cy3 images were segmented with Image Pro Plus software (Media Cybernetics, Bethesda, MD) and overlaid onto corresponding segmented DAPI images (Parsons et al. 2014). Objects with overlapping signals in both Cy3 and DAPI images were counted as probe positive. The negative control was determined similarly and subtracted from the positive probe counts to correct for autofluorescence and non-specific binding.\u00a0 (For Grazing Rates Processing, see below) Seawater picoplankton biomass for nucleic acids was taken from all experimental mesocosms on days 0, 2, 4, 6, 8, 10 and 12 of the experiment, and from coral mucus extracted from the experimental corals on days 2, 4, and 10 and processing followed a method modified from Giovannoni and colleagues (1990, 1996). 500 ml of water or 1 ml of coral mucus was filtered through a 47 mm, 0.2 um pore filter under gentle vacuum (100 mm Hg), placed into a 4 ml cryovial and stored in 1 ml of sterile sucrose lysis buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris.HCl) at -80 degrees celsius. For DNA extractions of the tissue, mucus and coral samples, sodium dodecyl sulfate to 1% and proteinase K to 200 ug ml-1 were added to the sample and incubated at 37 degrees celsius for 30 min and then at 55 degrees celsius for 30 min. The lysates were extracted with an equal volume of phenol:isoamylalcohol:chloroform (25:1:24) followed by two subsequent equal volumes of isoamylalcohol:chloroform (1:24). The DNA was purified by precipitation using sodium acetate (3M) and isopropanol (100%) for at least 1 hour at -20oC and centrifuged at room temperature for 30 min at 20,000 x g. The resulting pellet was washed with 80% ethanol, vortexed for 30 s and centrifuged at 16,000 x g for 10 min. The pellet was dried and stored at -20C.\u00a0 (See below for Microbial Processing description.) Seawater samples were analyzed for macronutrients (nitrate + nitrite, nitrite, ammonium, ortho phosphate, and silicic acid)\u00a0 at Oregon State University using a continuous segmented flow system consisting of a Technicon AutoAnalyzer II (SEAL Analytical) and an Alpkem RFA 300 Rapid Flow Analyzer (Alpkem) as conducted previously (Apprill and Rapp\u00e9 2011). DOC was determined via high temperature combustion on a modified Shimadzu TOC-V (Shimadzu Scientific Instruments, Columbia, MD) (Carlson et al. 2010). Flow cytometry was performed on 1 ml seawater preserved to a final concentration of 4% paraformaldehyde to enumerate pigmented picoeukaryotes, Synechococcus, and non-pigmented picoplankton using methods described in Apprill and Rapp\u00e9 (2011). High and low DNA-containing cells were enumerated following SybrGreen staining. The ANOVA and Tukey\u2019s HSD statistics were conducted as described below.
attribute	NC_GLOBAL	awards_0_award_nid	String	564441
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1233612
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233612
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Bacterioplankton data from aquaria experiments A. Apprill, PI Version 29 July 2016 Columns Measured with Flow Cytometry: nonPigmentPico,synechococcus,picoeukaryote, lowHeterotrophicBac, and highHeterotrophicBac Columns Measured with FISH: SAR11,rhodobacteracea,alteromonas,vibrio, thaumarchaeota, and euryarchaeota Column Units: Temperature: Celsius Salinity: PPT Flow Cytometry and FISH: cells per mL DAPI counts: cells per mL Growth and Grazing: day -1 Nutrients: micromoles
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-07-29T13:07:47Z
attribute	NC_GLOBAL	date_modified	String	2019-06-05T18:00:55Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.652849.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/652849
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Slides were enumerated using this microscope.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	652874
attribute	NC_GLOBAL	instruments_0_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_0_supplied_name	String	AX70 epifluorescent microscope
attribute	NC_GLOBAL	instruments_1_acronym	String	Thermal Cycler
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Reactions carried out on a Bio-Rad thermocycler.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	652875
attribute	NC_GLOBAL	instruments_1_description	String	General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. (adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute	NC_GLOBAL	instruments_1_instrument_name	String	PCR Thermal Cycler
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	471582
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Bio-Rad thermocycler
attribute	NC_GLOBAL	keywords	String	accession, accession_number, alteromonas, ammonia, ammonium, bac, bacGrazing_coefficient_mean, bacGrowth_constant_mean, bco, bco-dmo, biological, chemical, chemistry, coefficient, commerce, concentration, constant, count, dapi, DAPI_count, data, dataset, days, density, department, description, dmo, doc, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Salinity/Density > Salinity, erddap, euryarchaeota, grazing, growth, heterotrophic, high, highHeterotrophicBac, low, lowHeterotrophicBac, management, mean, mesocosm, mole, mole_concentration_of_ammonium_in_sea_water, n02, n03, N03_N02, name, nh4, non, nonPigmentPico, number, ocean, oceanography, oceans, office, p04, pico, picoeukaryote, pigment, practical, preliminary, rhodobacteracea, rhodobacteraceaGrazing_coefficient, rhodobacteraceaGrowth_constant_mean, salinity, sar11, SAR11Grazing_coefficient, SAR11Growth_constant_mean, science, sea, sea_water_practical_salinity, seawater, si04, synechococcus, synechococcusGrazing_coefficient, synechococcusGrowth_constant_mean, tank, temperature, thaumarchaeota, timepoint, timepoint_days, timepoint_name, treatment, treatment_description, vibrio, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/652849/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/652849
attribute	NC_GLOBAL	param_mapping	String	{'652849': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/652849/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Amy Apprill
attribute	NC_GLOBAL	people_0_person_nid	String	553489
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Coral Microbial Relationships
attribute	NC_GLOBAL	projects_0_acronym	String	Coral Microbial Relationships
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Reef-building corals are in decline worldwide due in part to climate change and other human activities, and it is becoming increasingly important to understand what aspects of coral biology are degraded by environmental stress which then leads to coral mortality. It is now widely known that corals harbor communities of bacteria and archaea that are believed to play important roles in maintaining the health of their hosts, but we lack any appreciable understanding about the identity of the microbial associates regularly residing within healthy, reef-building corals. This project asks the central question: do reef-building corals harbor fundamental or persistent microbial associates that are symbiotic within their tissues? In order to address this hypothesis, the investigator will assess the identity of the bacterial and archaeal microbes using a variety of molecular and microscopy approaches that includes the identification and localization of a widespread group of coral bacterial associates belonging to the genus Endozoicomonas. The results of this study will then be used to develop additional questions about the role of these microbial associates in nutrient cycling and how they contribute to the health and survival of corals.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Florida Keys, Federated States of Micronesia, Red Sea, & Bermuda
attribute	NC_GLOBAL	projects_0_name	String	Fundamental Coral-Microbial Associations
attribute	NC_GLOBAL	projects_0_project_nid	String	564442
attribute	NC_GLOBAL	projects_0_start_date	String	2012-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013
attribute	NC_GLOBAL	title	String	Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	treatment		String
attribute	treatment	bcodmo_name	String	treatment
attribute	treatment	description	String	mesocosm treatment; control; mucus addition; and coral with artifical lights
attribute	treatment	long_name	String	Treatment
attribute	treatment	units	String	unitless
variable	treatment_description		String
attribute	treatment_description	bcodmo_name	String	treatment
attribute	treatment_description	description	String	mesocosm condition; coral: presence or absence of colonies; mucus addition; or control
attribute	treatment_description	long_name	String	Treatment Description
attribute	treatment_description	units	String	unitless
variable	tank		byte
attribute	tank	_FillValue	byte	127
attribute	tank	actual_range	byte	1, 9
attribute	tank	bcodmo_name	String	tank
attribute	tank	description	String	tank number
attribute	tank	long_name	String	Tank
attribute	tank	units	String	unitless
variable	mesocosm		String
attribute	mesocosm	bcodmo_name	String	treatment
attribute	mesocosm	description	String	treatment and tank number
attribute	mesocosm	long_name	String	Mesocosm
attribute	mesocosm	units	String	unitless
variable	timepoint_name		String
attribute	timepoint_name	bcodmo_name	String	days
attribute	timepoint_name	description	String	day during duration of 12 day experiment
attribute	timepoint_name	long_name	String	Timepoint Name
attribute	timepoint_name	units	String	days
variable	timepoint_days		float
attribute	timepoint_days	_FillValue	float	NaN
attribute	timepoint_days	actual_range	float	0.0, 11.99
attribute	timepoint_days	bcodmo_name	String	days
attribute	timepoint_days	description	String	timepoint within 12 day experiment
attribute	timepoint_days	long_name	String	Timepoint Days
attribute	timepoint_days	units	String	days
variable	temp		float
attribute	temp	_FillValue	float	NaN
attribute	temp	actual_range	float	24.5, 26.9
attribute	temp	bcodmo_name	String	temperature
attribute	temp	description	String	temperature in mesocosm at each timepoint
attribute	temp	long_name	String	Temperature
attribute	temp	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	temp	units	String	celsius
variable	salinity		float
attribute	salinity	_FillValue	float	NaN
attribute	salinity	actual_range	float	36.32, 38.6
attribute	salinity	bcodmo_name	String	sal
attribute	salinity	colorBarMaximum	double	37.0
attribute	salinity	colorBarMinimum	double	32.0
attribute	salinity	description	String	salinity in mesocosm at each timepoint
attribute	salinity	long_name	String	Sea Water Practical Salinity
attribute	salinity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/
attribute	salinity	units	String	ppt
variable	DAPI_count		float
attribute	DAPI_count	_FillValue	float	NaN
attribute	DAPI_count	actual_range	float	359000.0, 2030000.0
attribute	DAPI_count	bcodmo_name	String	cell_concentration
attribute	DAPI_count	colorBarMaximum	double	100.0
attribute	DAPI_count	colorBarMinimum	double	0.0
attribute	DAPI_count	description	String	0 6-diamidino-2-phenyl dihydrochloride (DAPI) counts of picoplankton
attribute	DAPI_count	long_name	String	DAPI Count
attribute	DAPI_count	units	String	cells per milliliter
variable	accession_number		String
attribute	accession_number	bcodmo_name	String	accession number
attribute	accession_number	description	String	SSU rRNA gene V4 region sequences; NCBI SRA Accession
attribute	accession_number	long_name	String	Accession Number
attribute	accession_number	units	String	unitless
variable	nonPigmentPico		float
attribute	nonPigmentPico	_FillValue	float	NaN
attribute	nonPigmentPico	actual_range	float	204000.0, 7020000.0
attribute	nonPigmentPico	bcodmo_name	String	cell_concentration
attribute	nonPigmentPico	description	String	Non-pigmented picoplankton cells counted by flow cytometry
attribute	nonPigmentPico	long_name	String	Non Pigment Pico
attribute	nonPigmentPico	units	String	cells per milliliter
variable	synechococcus		int
attribute	synechococcus	_FillValue	int	2147483647
attribute	synechococcus	actual_range	int	39, 108386
attribute	synechococcus	bcodmo_name	String	cell_concentration
attribute	synechococcus	description	String	Synechococcus cells counted by flow cytometry
attribute	synechococcus	long_name	String	Synechococcus
attribute	synechococcus	units	String	cells per milliliter
variable	picoeukaryote		short
attribute	picoeukaryote	_FillValue	short	32767
attribute	picoeukaryote	actual_range	short	473, 9883
attribute	picoeukaryote	bcodmo_name	String	cell_concentration
attribute	picoeukaryote	description	String	picoeukaryote cells counted by flow cytometry
attribute	picoeukaryote	long_name	String	Picoeukaryote
attribute	picoeukaryote	units	String	cells per milliliter
variable	lowHeterotrophicBac		float
attribute	lowHeterotrophicBac	_FillValue	float	NaN
attribute	lowHeterotrophicBac	actual_range	float	8320.0, 1780000.0
attribute	lowHeterotrophicBac	bcodmo_name	String	cell_concentration
attribute	lowHeterotrophicBac	description	String	Low DNA heterotropic bacteria counted by flow cytometry
attribute	lowHeterotrophicBac	long_name	String	Low Heterotrophic Bac
attribute	lowHeterotrophicBac	units	String	cells per milliliter
variable	highHeterotrophicBac		float
attribute	highHeterotrophicBac	_FillValue	float	NaN
attribute	highHeterotrophicBac	actual_range	float	196000.0, 6550000.0
attribute	highHeterotrophicBac	bcodmo_name	String	cell_concentration
attribute	highHeterotrophicBac	description	String	High DNA concent heterotrophic bacteria counted by flow cytometry
attribute	highHeterotrophicBac	long_name	String	High Heterotrophic Bac
attribute	highHeterotrophicBac	units	String	cells per milliliter
variable	SAR11		float
attribute	SAR11	_FillValue	float	NaN
attribute	SAR11	actual_range	float	8310.0, 515000.0
attribute	SAR11	bcodmo_name	String	cell_concentration
attribute	SAR11	description	String	SAR11 cell counts by fluorescence in situ hybridization (FISH)
attribute	SAR11	long_name	String	SAR11
attribute	SAR11	units	String	cells per milliliter
variable	rhodobacteracea		float
attribute	rhodobacteracea	_FillValue	float	NaN
attribute	rhodobacteracea	actual_range	float	8670.0, 324000.0
attribute	rhodobacteracea	bcodmo_name	String	cell_concentration
attribute	rhodobacteracea	description	String	Rhodobacteracea cell counts by fluorescence in situ hybridization (FISH)
attribute	rhodobacteracea	long_name	String	Rhodobacteracea
attribute	rhodobacteracea	units	String	cells per milliliter
variable	alteromonas		float
attribute	alteromonas	_FillValue	float	NaN
attribute	alteromonas	actual_range	float	0.0, 98300.0
attribute	alteromonas	bcodmo_name	String	cell_concentration
attribute	alteromonas	description	String	Alteromonas cell counts by fluorescence in situ hybridization (FISH)
attribute	alteromonas	long_name	String	Alteromonas
attribute	alteromonas	units	String	cells per milliliter
variable	vibrio		float
attribute	vibrio	_FillValue	float	NaN
attribute	vibrio	actual_range	float	0.0, 55400.0
attribute	vibrio	bcodmo_name	String	cell_concentration
attribute	vibrio	description	String	Vibrio cell counts by fluorescence in situ hybridization (FISH)
attribute	vibrio	long_name	String	Vibrio
attribute	vibrio	units	String	cells per milliliter
variable	thaumarchaeota		float
attribute	thaumarchaeota	_FillValue	float	NaN
attribute	thaumarchaeota	actual_range	float	0.0, 80500.0
attribute	thaumarchaeota	bcodmo_name	String	cell_concentration
attribute	thaumarchaeota	description	String	Thaumarchaeota cell counts by fluorescence in situ hybridization (FISH)
attribute	thaumarchaeota	long_name	String	Thaumarchaeota
attribute	thaumarchaeota	units	String	cells per milliliter
variable	euryarchaeota		float
attribute	euryarchaeota	_FillValue	float	NaN
attribute	euryarchaeota	actual_range	float	0.0, 149000.0
attribute	euryarchaeota	bcodmo_name	String	cell_concentration
attribute	euryarchaeota	description	String	Euryarchaeota cell counts by fluorescence in situ hybridization (FISH)
attribute	euryarchaeota	long_name	String	Euryarchaeota
attribute	euryarchaeota	units	String	cells per milliliter
variable	P04		float
attribute	P04	_FillValue	float	NaN
attribute	P04	actual_range	float	0.03, 0.2
attribute	P04	bcodmo_name	String	PO4
attribute	P04	description	String	phosphate in seawater samples
attribute	P04	long_name	String	P04
attribute	P04	units	String	micromoles
variable	N03_N02		float
attribute	N03_N02	_FillValue	float	NaN
attribute	N03_N02	actual_range	float	0.52, 10.22
attribute	N03_N02	bcodmo_name	String	NO3_NO2
attribute	N03_N02	description	String	nitrate + nitrate in seawater samples
attribute	N03_N02	long_name	String	N03 N02
attribute	N03_N02	units	String	micromoles
variable	Si04		float
attribute	Si04	_FillValue	float	NaN
attribute	Si04	actual_range	float	1.1, 5.4
attribute	Si04	bcodmo_name	String	SiO4
attribute	Si04	description	String	silicate in seawater samples
attribute	Si04	long_name	String	Si04
attribute	Si04	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/
attribute	Si04	units	String	micromoles
variable	N02		float
attribute	N02	_FillValue	float	NaN
attribute	N02	actual_range	float	0.06, 0.48
attribute	N02	bcodmo_name	String	NO2
attribute	N02	description	String	nitrite in seawater samples
attribute	N02	long_name	String	N02
attribute	N02	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/
attribute	N02	units	String	micromoles
variable	NH4		float
attribute	NH4	_FillValue	float	NaN
attribute	NH4	actual_range	float	0.3, 7.09
attribute	NH4	bcodmo_name	String	Ammonium
attribute	NH4	colorBarMaximum	double	5.0
attribute	NH4	colorBarMinimum	double	0.0
attribute	NH4	description	String	ammonium in seawater samples
attribute	NH4	long_name	String	Mole Concentration Of Ammonium In Sea Water
attribute	NH4	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/
attribute	NH4	units	String	micromoles
variable	DOC		float
attribute	DOC	_FillValue	float	NaN
attribute	DOC	actual_range	float	71.82, 110.86
attribute	DOC	bcodmo_name	String	DOC
attribute	DOC	description	String	dissolved organic carbon in seawater samples
attribute	DOC	long_name	String	DOC
attribute	DOC	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/
attribute	DOC	units	String	micromoles
variable	bacGrowth_constant_mean		float
attribute	bacGrowth_constant_mean	_FillValue	float	NaN
attribute	bacGrowth_constant_mean	actual_range	float	-0.31, 0.5
attribute	bacGrowth_constant_mean	bcodmo_name	String	mean
attribute	bacGrowth_constant_mean	description	String	k; mean bacterial growth constant
attribute	bacGrowth_constant_mean	long_name	String	Bac Growth Constant Mean
attribute	bacGrowth_constant_mean	units	String	days -1
variable	bacGrazing_coefficient_mean		float
attribute	bacGrazing_coefficient_mean	_FillValue	float	NaN
attribute	bacGrazing_coefficient_mean	actual_range	float	-0.32, 0.56
attribute	bacGrazing_coefficient_mean	bcodmo_name	String	mean
attribute	bacGrazing_coefficient_mean	description	String	g; mean bact grazing coefficient
attribute	bacGrazing_coefficient_mean	long_name	String	Bac Grazing Coefficient Mean
attribute	bacGrazing_coefficient_mean	units	String	days -1
variable	synechococcusGrowth_constant_mean		float
attribute	synechococcusGrowth_constant_mean	_FillValue	float	NaN
attribute	synechococcusGrowth_constant_mean	actual_range	float	-1.18, 0.03
attribute	synechococcusGrowth_constant_mean	bcodmo_name	String	mean
attribute	synechococcusGrowth_constant_mean	description	String	k; mean synechococcus growth constant
attribute	synechococcusGrowth_constant_mean	long_name	String	Synechococcus Growth Constant Mean
attribute	synechococcusGrowth_constant_mean	units	String	days -1
variable	synechococcusGrazing_coefficient		float
attribute	synechococcusGrazing_coefficient	_FillValue	float	NaN
attribute	synechococcusGrazing_coefficient	actual_range	float	-1.21, -0.55
attribute	synechococcusGrazing_coefficient	bcodmo_name	String	unknown
attribute	synechococcusGrazing_coefficient	description	String	synechococcus grazing coefficient
attribute	synechococcusGrazing_coefficient	long_name	String	Synechococcus Grazing Coefficient
attribute	synechococcusGrazing_coefficient	units	String	days -1
variable	SAR11Growth_constant_mean		float
attribute	SAR11Growth_constant_mean	_FillValue	float	NaN
attribute	SAR11Growth_constant_mean	actual_range	float	-0.91, 1.19
attribute	SAR11Growth_constant_mean	bcodmo_name	String	mean
attribute	SAR11Growth_constant_mean	description	String	k; mean SAR11 growth constant
attribute	SAR11Growth_constant_mean	long_name	String	SAR11 Growth Constant Mean
attribute	SAR11Growth_constant_mean	units	String	days -1
variable	SAR11Grazing_coefficient		float
attribute	SAR11Grazing_coefficient	_FillValue	float	NaN
attribute	SAR11Grazing_coefficient	actual_range	float	-0.98, -0.26
attribute	SAR11Grazing_coefficient	bcodmo_name	String	unknown
attribute	SAR11Grazing_coefficient	description	String	SAR11 grazing coefficient
attribute	SAR11Grazing_coefficient	long_name	String	SAR11 Grazing Coefficient
attribute	SAR11Grazing_coefficient	units	String	days -1
variable	rhodobacteraceaGrowth_constant_mean		float
attribute	rhodobacteraceaGrowth_constant_mean	_FillValue	float	NaN
attribute	rhodobacteraceaGrowth_constant_mean	actual_range	float	-0.89, 1.34
attribute	rhodobacteraceaGrowth_constant_mean	bcodmo_name	String	mean
attribute	rhodobacteraceaGrowth_constant_mean	description	String	k; mean Rhodobacteracea growth constant
attribute	rhodobacteraceaGrowth_constant_mean	long_name	String	Rhodobacteracea Growth Constant Mean
attribute	rhodobacteraceaGrowth_constant_mean	units	String	days -1
variable	rhodobacteraceaGrazing_coefficient		float
attribute	rhodobacteraceaGrazing_coefficient	_FillValue	float	NaN
attribute	rhodobacteraceaGrazing_coefficient	actual_range	float	-0.92, 0.06
attribute	rhodobacteraceaGrazing_coefficient	bcodmo_name	String	unknown
attribute	rhodobacteraceaGrazing_coefficient	description	String	Rhodobacteracea grazing coefficient
attribute	rhodobacteraceaGrazing_coefficient	long_name	String	Rhodobacteracea Grazing Coefficient
attribute	rhodobacteraceaGrazing_coefficient	units	String	days -1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.