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Dataset Title:  CO2 \u00d7 temperature specific early life survival and growth of Menidia
menidia assessed by 5 factorial experiments
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_732818)
Range: longitude = -73.10258 to -72.01524°E, latitude = 40.947376 to 41.321526°N
Information:  Summary ? | License ? | ISO 19115 | Metadata | Background (external link) | Subset | Data Access Form | Files
 
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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  experiment {
    Byte _FillValue 127;
    Byte actual_range 1, 5;
    String description "Experiment number";
    String ioos_category "Unknown";
    String long_name "Experiment";
    String units "unitless";
  }
  species {
    String description "Scientific name of the speices (Menidia menidia, the Atlantic silverside)";
    String ioos_category "Taxonomy";
    String long_name "Species";
    String units "unitless";
  }
  adult_collection_site {
    String description "Site of the collection of wild adult spawners";
    String ioos_category "Unknown";
    String long_name "Adult Collection Site";
    String units "unitless";
  }
  latitude {
    String _CoordinateAxisType "Lat";
    Float64 _FillValue NaN;
    Float64 actual_range 40.947376, 41.321526;
    String axis "Y";
    Float64 colorBarMaximum 90.0;
    Float64 colorBarMinimum -90.0;
    String description "Latitude of adult collection site";
    String ioos_category "Location";
    String long_name "Latitude";
    String standard_name "latitude";
    String units "degrees_north";
  }
  longitude {
    String _CoordinateAxisType "Lon";
    Float64 _FillValue NaN;
    Float64 actual_range -73.10258, -72.015247;
    String axis "X";
    Float64 colorBarMaximum 180.0;
    Float64 colorBarMinimum -180.0;
    String description "Longitude of adult collection site";
    String ioos_category "Location";
    String long_name "Longitude";
    String standard_name "longitude";
    String units "degrees_east";
  }
  tank {
    String description "Tank number";
    String ioos_category "Unknown";
    String long_name "Tank";
    String units "unitless";
  }
  replicate {
    Byte _FillValue 127;
    Byte actual_range 1, 98;
    String description "Within experiment replicate number";
    String ioos_category "Unknown";
    String long_name "Replicate";
    Byte missing_value 99;
    String units "unitless";
  }
  temperature {
    Float32 _FillValue NaN;
    Float32 actual_range 16.9, 28.2;
    String description "Average temperature experienced by fish in degrees celsius";
    String ioos_category "Temperature";
    String long_name "Temperature";
    String units "degrees Celsius";
  }
  pH {
    Float32 _FillValue NaN;
    Float32 actual_range 7.14, 8.24;
    Float64 colorBarMaximum 9.0;
    Float64 colorBarMinimum 7.0;
    String description "Average pH level experienced by the fish NBS";
    String ioos_category "Salinity";
    String long_name "Sea Water Ph Reported On Total Scale";
    String units "pH NBS";
  }
  pCO2 {
    Int16 _FillValue 32767;
    Int16 actual_range 322, 6195;
    String description "Calculated average pCO2 levels in �atm calculated by CO2SYS based on alkalinity, pH, and temperature";
    String ioos_category "CO2";
    String long_name "P CO2";
    String units "atm";
  }
  fertilization_date {
    String description "Date offspring were fertilized by stripspawning 20+ adults of each sex; formatted as yyyy/mm/dd";
    String ioos_category "Time";
    String long_name "Fertilization Date";
    String source_name "fertilization_date";
    String units "unitless";
  }
  hatch_sample_date {
    String description "Date offspring were sampled for hatch survival and measurements; formatted as yyyy/mm/dd";
    String ioos_category "Time";
    String long_name "Hatch Sample Date";
    String units "unitless";
  }
  hatch_sample_age {
    Byte _FillValue 127;
    Byte actual_range 5, 15;
    String description "Age in days post fertilization (dpf) on the date of hatch sampling";
    String ioos_category "Time";
    String long_name "Hatch Sample Age";
    String units "days";
  }
  final_sample_date {
    String description "Date offspring were sampled for final survival and measurements; formatted as yyyy/mm/dd";
    String ioos_category "Time";
    String long_name "Final Sample Date";
    String units "unitless";
  }
  final_sample_age {
    Byte _FillValue 127;
    Byte actual_range 14, 36;
    String description "Age in days post fertilization (dpf) on the date of final sampling";
    String ioos_category "Time";
    String long_name "Final Sample Age";
    String units "days";
  }
  rel_embryo_survival {
    Float32 _FillValue NaN;
    Float32 actual_range 0.39, 1.0;
    String description "relative survival of embryos (0-1)";
    String ioos_category "Unknown";
    String long_name "Rel Embryo Survival";
    String units "unitless (fraction)";
  }
  rel_larval_survival {
    Float32 _FillValue NaN;
    Float32 actual_range 0.03, 1.0;
    String description "relative survival from hatch to experiment termination (0-1)";
    String ioos_category "Biology";
    String long_name "Rel Larval Survival";
    String units "unitless (fraction)";
  }
  hatch_length {
    Float32 _FillValue NaN;
    Float32 actual_range 4.42, 5.74;
    String description "Average replicate hatch length (mm)";
    String ioos_category "Unknown";
    String long_name "Hatch Length";
    String units "millimeters (mm)";
  }
  final_length {
    Float32 _FillValue NaN;
    Float32 actual_range 6.95, 10.57;
    String description "Average replicate final length (mm)";
    String ioos_category "Unknown";
    String long_name "Final Length";
    String units "millimeters (mm)";
  }
  growth_rate {
    Float32 _FillValue NaN;
    Float32 actual_range 0.14, 0.56;
    String description "average replicate growth rate (mm d-1) of offspring from hatch to experiment termination";
    String ioos_category "Unknown";
    String long_name "Growth Rate";
    String units "millimeters per day (mm d-1)";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson";
    String acquisition_description 
"CO2 \\u00d7 temperature manipulations and measurements:  
 For 2 \\u00d7 2 and 3 \\u00d7 2 factorial designs, replicate rearing
containers (20 L) were placed into large temperature-controlled water baths.
Elevated CO2 levels were achieved via gas proportioners (ColeParmer\\u00ae)
mixing air with 100% CO2 (bone dry grade) that was delivered continuously to
the bottom of each replicate rearing container via airstone. To counteract
metabolic CO2 accumulation, control CO2 conditions were achieved by forcing
compressed laboratory air through a series of CO2 stripping units containing
granular soda lime (AirGas\\u00ae), a particle filter (1 \\u00b5m), and then to
each replicate via airstone. Target pH levels were monitored daily using a
handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH
Portable Meter; Intellical PHC281 pH Electrode with Hach\\u00ae HQ11D Handheld
pH/ORP Meter) calibrated bi-weekly with 2-point pHNBS references. Continuous
bubbling maintained dissolved oxygen saturation (>8 mg/ DO) in rearing
vessels. Target treatment temperatures were controlled by thermostats
(Aqualogic\\u00ae) which powered chillers (DeltaStar\\u00ae) or glass
submersible heaters to maintain water bath temperatures. For 3 \\u00d7 3
factorial experiments, we developed an automated acidification system composed
of nine discrete recirculation units designed for larval fish rearing. We
designed a LabView (National Instruments\\u00ae) based program to fully
automate the control of seawater chemistry. The software interfaces with the
recirculating units via a data-acquisition module (NI cDAQ-9184, National
Instruments\\u00ae), which controls nine sampling pumps (one per tank) and a
series of gas and water solenoid valves, while receiving input from a central
pH electrode (Hach pHD\\u00ae digital electrode calibrated weekly using 2-point
pHNBS references) and dissolved oxygen probe (Hach LDO\\u00ae Model 2). The
software sequentially assesses the pH conditions in each rearing unit (each
tank once per hour) by pumping water for ~450 seconds through the housing of
the central pH probe, comparing measured pH levels to set-points and then
adjusting levels by bubbling standardized amounts 100% CO2 (bone dry grade,
AirGas\\u00ae) or CO2-stripped air into the sump of each tank. The software
also maintains DO saturation (>8 mg/l) by bubbling in CO2-stripped air.
LabView logs current pH, temperature, and DO conditions before cycling to the
next unit. Temperatures were controlled by thermostats (Aqualogic\\u00ae) that
powered submersible heaters or in-line chillers (DeltaStar\\u00ae).
 
Actual treatment CO2 levels were determined based on measurements of pH,
temperature, salinity, and total alkalinity (AT). Treatment tanks were sampled
three times per experiment for measurements of AT (\\u03bcmol kg-1). Seawater
was siphoned and filtered (to 10 \\u00b5m) into 300 ml borosilicate bottles.
Salinity was measured at the time of sampling using a refractometer. Bottles
were stored at 3\\u02daC and measured for AT within two weeks of sampling using
an endpoint titration (Mettler Toledo\\u00ae\\u00a0G20 Potentiometric Titrator).
Methodological accuracy (within \\u00b11%) of alkalinity titrations were
verified and calibrated using Dr. Andrew Dickson\\u2019s (University of
California San Diego, Scripps Institution of Oceanography) certified reference
material for AT in seawater. The partial pressure of CO2 (pCO2,; \\u03bcatm)
was calculated in CO2SYS (V2.1,
[http://cdiac.ornl.gov/ftp/co2sys](\\\\\"http://cdiac.ornl.gov/ftp/co2sys\\\\\"))
based on measured AT, pHNBS, temperature, and salinity using K1 and K2
constants from Mehrbach et al. (1973)\\u00a0 refit by Dickson and Millero
(1987)\\u00a0 and Dickson (1990) for KHSO4.
 
Field sampling and experimental designs:  
 Collections of wild, spawning ripe Atlantic silversides were made during
high tide 1-3 days prior to full or new moons during the species spawning
season. Adults were caught with a 30 m \\u00d7 2 m beach seine from local salt
marshes and transported live to our laboratory facilities. Ripe adults were
held overnight at 20\\u00b0C in well aerated tanks at low densities with no
food and strip spawned the next day.
 
For each experiment, eggs from 20+ running-ripe females were gently mixed into
shallow plastic dishes lined with 1 mm plastic window screening. 20+ males
were stripped-spawned together into 500 ml glass beakers, mixed with seawater,
stirred, then gently poured into spawning dishes and mixed with eggs for ~15
minutes. Screens were rinsed with seawater to remove unfertilized eggs and
then soaked in a 100 ppm buffered iodine (Ovadine\\u00ae) solution for 15
minutes to prevent fungal infection. Experiments were initiated within two
hours of fertilization when replicate rearing vessels received precisely 100
embryos. Vessels were filled with clean seawater (filtered to 1 \\u00b5m and UV
sterilized). Optimal salinity (27-31) and light conditions (15 h light:9 h
dark) for rearing M. menidia were maintained across experiments. Upon hatching
larvae were immediately provided ad libitum rations of newly hatched brine
shrimp nauplii (Artemia salina, San Francisco strain, brineshrimpdirect.com)
and equal rations of powdered weaning diet (Otohime Marine Fish Diet, size A1,
Reed Mariculture\\u00ae). To quantify hatching survival, one day post first
hatch larvae were counted by gently scooping small groups into replacement
rearing vessels. For initial hatch measurements, random sub-samples (N = 10)
from each replicate were preserved in 5% formaldehyde/freshwater solution
buffered with saturated sodium tetraborate. All experiments were terminated
when larvae reached ~10 mm standard length (SL). At termination, all survivors
were counted and measured for standard length (SL, nearest 0.01 mm) via
calibrated digital images (Image Pro Premier\\u00ae V9.0).";
    String awards_0_award_nid "650183";
    String awards_0_award_number "OCE-1536336";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536336";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "Dr Michael E. Sieracki";
    String awards_0_program_manager_nid "50446";
    String cdm_data_type "Other";
    String comment 
"CO2 x temperature effects on Menidia menidia offspring 
  PI: Hannes Baumann (UConn) 
  Co-PIs: Christopher Murray (UConn) & Janet Nye (SUNY Stony Brook) 
  Version: 05 April 2018";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv.pl v1.0";
    String date_created "2018-04-03T18:40:18Z";
    String date_modified "2018-07-24T16:37:11Z";
    String defaultDataQuery "&time";
    String doi "10.1575/1912/bco-dmo.742200";
    Float64 Easternmost_Easting -72.015247;
    Float64 geospatial_lat_max 41.321526;
    Float64 geospatial_lat_min 40.947376;
    String geospatial_lat_units "degrees_north";
    Float64 geospatial_lon_max -72.015247;
    Float64 geospatial_lon_min -73.10258;
    String geospatial_lon_units "degrees_east";
    String history 
"2019-06-16T02:43:12Z (local files)
2019-06-16T02:43:12Z https://erddap.bco-dmo.org/tabledap/bcodmo_dataset_732818.das";
    String infoUrl "https://www.bco-dmo.org/dataset/732818";
    String institution "BCO-DMO";
    String instruments_0_acronym "pH Sensor";
    String instruments_0_dataset_instrument_description "Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach�HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pHNBS references.";
    String instruments_0_dataset_instrument_nid "732828";
    String instruments_0_description "General term for an instrument that measures the pH or how acidic or basic a solution is.";
    String instruments_0_instrument_name "pH Sensor";
    String instruments_0_instrument_nid "674";
    String instruments_0_supplied_name "handheld pH probe";
    String instruments_1_acronym "Refractometer";
    String instruments_1_dataset_instrument_description "Salinity was measured at the time of sampling using a refractometer.";
    String instruments_1_dataset_instrument_nid "732830";
    String instruments_1_description 
"A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.

In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.";
    String instruments_1_instrument_name "Refractometer";
    String instruments_1_instrument_nid "679";
    String instruments_1_supplied_name "refractometer";
    String instruments_2_acronym "Automatic titrator";
    String instruments_2_dataset_instrument_description "Bottles were stored at 3˚C and measured for AT within two weeks of sampling using an endpoint titration (Mettler Toledo G20 Potentiometric Titrator).�";
    String instruments_2_dataset_instrument_nid "732831";
    String instruments_2_description "Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.";
    String instruments_2_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB12/";
    String instruments_2_instrument_name "Automatic titrator";
    String instruments_2_instrument_nid "682";
    String instruments_2_supplied_name "Mettler Toledo G20 Potentiometric Titrator";
    String instruments_3_acronym "Dissolved Oxygen Sensor";
    String instruments_3_dataset_instrument_nid "732829";
    String instruments_3_description "An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed";
    String instruments_3_instrument_name "Dissolved Oxygen Sensor";
    String instruments_3_instrument_nid "705";
    String instruments_3_supplied_name "dissolved oxygen probe";
    String instruments_4_acronym "Airstone";
    String instruments_4_dataset_instrument_description "Elevated CO2 levels were achieved via gas proportioners (ColeParmer) mixing air with 100% CO2 (bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone.";
    String instruments_4_dataset_instrument_nid "732827";
    String instruments_4_description "Airstone - Also called an aquarium bubbler, is a piece of aquarium furniture, traditionally a piece of limewood or porous stone, whose purpose is to gradually diffuse air into the tank, eliminating the noise and large bubbles of conventional air filtration systems";
    String instruments_4_instrument_name "Airstone";
    String instruments_4_instrument_nid "713";
    String instruments_4_supplied_name "airstone";
    String instruments_5_acronym "Immersion heater";
    String instruments_5_dataset_instrument_description "Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures.";
    String instruments_5_dataset_instrument_nid "732834";
    String instruments_5_description "Submersible heating element for water tanks and aquaria.";
    String instruments_5_instrument_name "Immersion heater";
    String instruments_5_instrument_nid "522981";
    String instruments_5_supplied_name "glass submersible heaters";
    String instruments_6_acronym "Aquarium chiller";
    String instruments_6_dataset_instrument_description "Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures.";
    String instruments_6_dataset_instrument_nid "732833";
    String instruments_6_description "Immersible or in-line liquid cooling device, usually with temperature control.";
    String instruments_6_instrument_name "Aquarium chiller";
    String instruments_6_instrument_nid "522982";
    String instruments_6_supplied_name "DeltaStar chiller";
    String instruments_7_dataset_instrument_description "Adults were caught with a 30 m � 2 m beach seine from local salt marshes and transported live to our laboratory facilities.�";
    String instruments_7_dataset_instrument_nid "732832";
    String instruments_7_description 
"A seine net is a very long net, with or without a bag in the centre, which is set either from the shore or from a boat for surrounding a certain area and is operated with two (long) ropes fixed to its ends (for hauling and herding the fish).

Seine nets are operated both in inland and in marine waters. The surrounded and catching area depends on the length of the seine and of the hauling lines.

(definition from: fao.org)";
    String instruments_7_instrument_name "Seine Net";
    String instruments_7_instrument_nid "716403";
    String instruments_7_supplied_name "beach seine";
    String keywords "adult, adult_collection_site, age, bco, bco-dmo, biological, biology, carbon, carbon dioxide, chemical, chemistry, co2, collection, data, dataset, date, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, embryo, erddap, experiment, fertilization, final, final_length, final_sample_age, final_sample_date, growth, growth_rate, hatch, hatch_length, hatch_sample_age, hatch_sample_date, larval, latitude, length, longitude, management, ocean, oceanography, oceans, office, pCO2, preliminary, rate, rel, rel_embryo_survival, rel_larval_survival, replicate, reported, salinity, sample, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, site, species, survival, tank, taxonomy, temperature, time, total, water";
    String keywords_vocabulary "GCMD Science Keywords";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    String metadata_source "https://www.bco-dmo.org/api/dataset/732818";
    Float64 Northernmost_Northing 41.321526;
    String param_mapping "{'732818': {'latitude': 'flag - latitude', 'longitude': 'flag - longitude'}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/732818/parameters";
    String people_0_affiliation "University of Connecticut";
    String people_0_affiliation_acronym "UConn";
    String people_0_person_name "Hannes Baumann";
    String people_0_person_nid "528586";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "University of Connecticut";
    String people_1_affiliation_acronym "UConn - Avery Point";
    String people_1_person_name "Christopher S. Murray";
    String people_1_person_nid "742121";
    String people_1_role "Co-Principal Investigator";
    String people_1_role_type "originator";
    String people_2_affiliation "Stony Brook University - SoMAS";
    String people_2_affiliation_acronym "SUNY-SB SoMAS";
    String people_2_person_name "Janet Nye";
    String people_2_person_nid "650186";
    String people_2_role "Co-Principal Investigator";
    String people_2_role_type "originator";
    String people_3_affiliation "University of Connecticut";
    String people_3_affiliation_acronym "UConn";
    String people_3_person_name "Hannes Baumann";
    String people_3_person_nid "528586";
    String people_3_role "Contact";
    String people_3_role_type "related";
    String people_4_affiliation "Woods Hole Oceanographic Institution";
    String people_4_affiliation_acronym "WHOI BCO-DMO";
    String people_4_person_name "Shannon Rauch";
    String people_4_person_nid "51498";
    String people_4_role "BCO-DMO Data Manager";
    String people_4_role_type "related";
    String project "Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish";
    String projects_0_acronym "HYPOA";
    String projects_0_description 
"Description from NSF award abstract:
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the \"Women in Science and Engineering\" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.
This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 � DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 � 3 DO levels. Second, the effects of tidal and diel CO2 � DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature � CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.
Related references:
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:10.1098/rsbl.2015.0976
Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:10.1002/loe2.10002
Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:10.3354/meps11142";
    String projects_0_end_date "2018-11";
    String projects_0_geolocation "Eastern Long Island Sound, CT, USA";
    String projects_0_name "Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish";
    String projects_0_project_nid "650184";
    String projects_0_start_date "2015-12";
    String publisher_name "Shannon Rauch";
    String publisher_role "BCO-DMO Data Manager(s)";
    String sourceUrl "(local files)";
    Float64 Southernmost_Northing 40.947376;
    String standard_name_vocabulary "CF Standard Name Table v29";
    String subsetVariables "species";
    String summary "In five individual rearing experiments, wild-caught M. menidia adults were spawned to test offspring sensitivity to factorial combinations of pCO2 (nominal: 400, 2200, 4000, and 6000 \\u00b5atm) and temperature (17, 20, 24, and 28 \\u00b0C) through measurements of early-life survival and growth. For experiment 1, adults were collected from Poquot Beach (40.947376, -73.10258), and the experiment took place at Stony Brook University\\u2019s Flax Pond Marine Laboratory. For experiments 2\\u20135, spawning adults were collected from Mumford Cove (41.321526, -72.015247), and experiments were conducted in the Rankin Seawater Facility at University of Connecticut\\u2019s Avery Point campus. The experiments quantified two survival and two growth traits for each replicate and CO2 \\u00d7 temperature treatment; embryo survival (fertilization to 1 dph), larval survival (1 dph to experiment termination), size (SL) at hatch (1 dph), and growth rate ((SL at end of experiment \\u2013 SL 1dph)/number days reared post hatch). These data are published in: Murray, C.S., and Baumann, H. (2018) You Better Repeat It: Complex CO2 \\u00d7 Temperature Effects in Atlantic Silverside Offspring Revealed by Serial experimentation. Diversity. doi:10.3390/d10030069.";
    String title "CO2 \\u00d7 temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments";
    String version "1";
    Float64 Westernmost_Easting -73.10258;
    String xml_source "osprey2erddap.update_xml() v1.0-alpha";
  }
}

 

Using tabledap to Request Data and Graphs from Tabular Datasets

tabledap lets you request a data subset, a graph, or a map from a tabular dataset (for example, buoy data), via a specially formed URL. tabledap uses the OPeNDAP (external link) Data Access Protocol (DAP) (external link) and its selection constraints (external link).

The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.

Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names, followed by a collection of constraints (e.g., variable<value), each preceded by '&' (which is interpreted as "AND").

For details, see the tabledap Documentation.


 
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