ERDDAP > info > bcodmo_dataset_732818

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[CO2 x temperature effects on Menidia menidia offspring] - CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments (Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish)		F   I   M	background			BCO-DMO	bcodmo_dataset_732818

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	CO2 \u00d7 temperature manipulations and measurements: For 2 \u00d7 2 and 3 \u00d7 2 factorial designs, replicate rearing containers (20 L) were placed into large temperature-controlled water baths. Elevated CO2 levels were achieved via gas proportioners (ColeParmer\u00ae) mixing air with 100% CO2 (bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone. To counteract metabolic CO2 accumulation, control CO2 conditions were achieved by forcing compressed laboratory air through a series of CO2 stripping units containing granular soda lime (AirGas\u00ae), a particle filter (1 \u00b5m), and then to each replicate via airstone. Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach\u00ae HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pHNBS references. Continuous bubbling maintained dissolved oxygen saturation (>8 mg/ DO) in rearing vessels. Target treatment temperatures were controlled by thermostats (Aqualogic\u00ae) which powered chillers (DeltaStar\u00ae) or glass submersible heaters to maintain water bath temperatures. For 3 \u00d7 3 factorial experiments, we developed an automated acidification system composed of nine discrete recirculation units designed for larval fish rearing. We designed a LabView (National Instruments\u00ae) based program to fully automate the control of seawater chemistry. The software interfaces with the recirculating units via a data-acquisition module (NI cDAQ-9184, National Instruments\u00ae), which controls nine sampling pumps (one per tank) and a series of gas and water solenoid valves, while receiving input from a central pH electrode (Hach pHD\u00ae digital electrode calibrated weekly using 2-point pHNBS references) and dissolved oxygen probe (Hach LDO\u00ae Model 2). The software sequentially assesses the pH conditions in each rearing unit (each tank once per hour) by pumping water for ~450 seconds through the housing of the central pH probe, comparing measured pH levels to set-points and then adjusting levels by bubbling standardized amounts 100% CO2 (bone dry grade, AirGas\u00ae) or CO2-stripped air into the sump of each tank. The software also maintains DO saturation (>8 mg/l) by bubbling in CO2-stripped air. LabView logs current pH, temperature, and DO conditions before cycling to the next unit. Temperatures were controlled by thermostats (Aqualogic\u00ae) that powered submersible heaters or in-line chillers (DeltaStar\u00ae). Actual treatment CO2 levels were determined based on measurements of pH, temperature, salinity, and total alkalinity (AT). Treatment tanks were sampled three times per experiment for measurements of AT (\u03bcmol kg-1). Seawater was siphoned and filtered (to 10 \u00b5m) into 300 ml borosilicate bottles. Salinity was measured at the time of sampling using a refractometer. Bottles were stored at 3\u02daC and measured for AT within two weeks of sampling using an endpoint titration (Mettler Toledo\u00ae\u00a0G20 Potentiometric Titrator). Methodological accuracy (within \u00b11%) of alkalinity titrations were verified and calibrated using Dr. Andrew Dickson\u2019s (University of California San Diego, Scripps Institution of Oceanography) certified reference material for AT in seawater. The partial pressure of CO2 (pCO2,; \u03bcatm) was calculated in CO2SYS (V2.1, [http://cdiac.ornl.gov/ftp/co2sys](\\"http://cdiac.ornl.gov/ftp/co2sys\\")) based on measured AT, pHNBS, temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973)\u00a0 refit by Dickson and Millero (1987)\u00a0 and Dickson (1990) for KHSO4. Field sampling and experimental designs: Collections of wild, spawning ripe Atlantic silversides were made during high tide 1-3 days prior to full or new moons during the species spawning season. Adults were caught with a 30 m \u00d7 2 m beach seine from local salt marshes and transported live to our laboratory facilities. Ripe adults were held overnight at 20\u00b0C in well aerated tanks at low densities with no food and strip spawned the next day. For each experiment, eggs from 20+ running-ripe females were gently mixed into shallow plastic dishes lined with 1 mm plastic window screening. 20+ males were stripped-spawned together into 500 ml glass beakers, mixed with seawater, stirred, then gently poured into spawning dishes and mixed with eggs for ~15 minutes. Screens were rinsed with seawater to remove unfertilized eggs and then soaked in a 100 ppm buffered iodine (Ovadine\u00ae) solution for 15 minutes to prevent fungal infection. Experiments were initiated within two hours of fertilization when replicate rearing vessels received precisely 100 embryos. Vessels were filled with clean seawater (filtered to 1 \u00b5m and UV sterilized). Optimal salinity (27-31) and light conditions (15 h light:9 h dark) for rearing M. menidia were maintained across experiments. Upon hatching larvae were immediately provided ad libitum rations of newly hatched brine shrimp nauplii (Artemia salina, San Francisco strain, brineshrimpdirect.com) and equal rations of powdered weaning diet (Otohime Marine Fish Diet, size A1, Reed Mariculture\u00ae). To quantify hatching survival, one day post first hatch larvae were counted by gently scooping small groups into replacement rearing vessels. For initial hatch measurements, random sub-samples (N = 10) from each replicate were preserved in 5% formaldehyde/freshwater solution buffered with saturated sodium tetraborate. All experiments were terminated when larvae reached ~10 mm standard length (SL). At termination, all survivors were counted and measured for standard length (SL, nearest 0.01 mm) via calibrated digital images (Image Pro Premier\u00ae V9.0).
attribute	NC_GLOBAL	awards_0_award_nid	String	650183
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1536336
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536336
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	CO2 x temperature effects on Menidia menidia offspring PI: Hannes Baumann (UConn) Co-PIs: Christopher Murray (UConn) & Janet Nye (SUNY Stony Brook) Version: 05 April 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-04-03T18:40:18Z
attribute	NC_GLOBAL	date_modified	String	2018-07-24T16:37:11Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.742200
attribute	NC_GLOBAL	Easternmost_Easting	double	-72.015247
attribute	NC_GLOBAL	geospatial_lat_max	double	41.321526
attribute	NC_GLOBAL	geospatial_lat_min	double	40.947376
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-72.015247
attribute	NC_GLOBAL	geospatial_lon_min	double	-73.10258
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/732818
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pHNBS references.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	732828
attribute	NC_GLOBAL	instruments_0_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_0_supplied_name	String	handheld pH probe
attribute	NC_GLOBAL	instruments_1_acronym	String	Refractometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Salinity was measured at the time of sampling using a refractometer.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	732830
attribute	NC_GLOBAL	instruments_1_description	String	A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation. In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Refractometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	679
attribute	NC_GLOBAL	instruments_1_supplied_name	String	refractometer
attribute	NC_GLOBAL	instruments_2_acronym	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Bottles were stored at 3˚C and measured for AT within two weeks of sampling using an endpoint titration (Mettler Toledo G20 Potentiometric Titrator).
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	732831
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB12/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	682
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Mettler Toledo G20 Potentiometric Titrator
attribute	NC_GLOBAL	instruments_3_acronym	String	Dissolved Oxygen Sensor
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	732829
attribute	NC_GLOBAL	instruments_3_description	String	An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Dissolved Oxygen Sensor
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	705
attribute	NC_GLOBAL	instruments_3_supplied_name	String	dissolved oxygen probe
attribute	NC_GLOBAL	instruments_4_acronym	String	Airstone
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Elevated CO2 levels were achieved via gas proportioners (ColeParmer) mixing air with 100% CO2 (bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	732827
attribute	NC_GLOBAL	instruments_4_description	String	Airstone - Also called an aquarium bubbler, is a piece of aquarium furniture, traditionally a piece of limewood or porous stone, whose purpose is to gradually diffuse air into the tank, eliminating the noise and large bubbles of conventional air filtration systems
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Airstone
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	713
attribute	NC_GLOBAL	instruments_4_supplied_name	String	airstone
attribute	NC_GLOBAL	instruments_5_acronym	String	Immersion heater
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures.
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	732834
attribute	NC_GLOBAL	instruments_5_description	String	Submersible heating element for water tanks and aquaria.
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Immersion heater
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	522981
attribute	NC_GLOBAL	instruments_5_supplied_name	String	glass submersible heaters
attribute	NC_GLOBAL	instruments_6_acronym	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_6_dataset_instrument_description	String	Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures.
attribute	NC_GLOBAL	instruments_6_dataset_instrument_nid	String	732833
attribute	NC_GLOBAL	instruments_6_description	String	Immersible or in-line liquid cooling device, usually with temperature control.
attribute	NC_GLOBAL	instruments_6_instrument_name	String	Aquarium chiller
attribute	NC_GLOBAL	instruments_6_instrument_nid	String	522982
attribute	NC_GLOBAL	instruments_6_supplied_name	String	DeltaStar chiller
attribute	NC_GLOBAL	instruments_7_dataset_instrument_description	String	Adults were caught with a 30 m × 2 m beach seine from local salt marshes and transported live to our laboratory facilities.
attribute	NC_GLOBAL	instruments_7_dataset_instrument_nid	String	732832
attribute	NC_GLOBAL	instruments_7_description	String	A seine net is a very long net, with or without a bag in the centre, which is set either from the shore or from a boat for surrounding a certain area and is operated with two (long) ropes fixed to its ends (for hauling and herding the fish). Seine nets are operated both in inland and in marine waters. The surrounded and catching area depends on the length of the seine and of the hauling lines. (definition from: fao.org)
attribute	NC_GLOBAL	instruments_7_instrument_name	String	Seine Net
attribute	NC_GLOBAL	instruments_7_instrument_nid	String	716403
attribute	NC_GLOBAL	instruments_7_supplied_name	String	beach seine
attribute	NC_GLOBAL	keywords	String	adult, adult_collection_site, age, bco, bco-dmo, biological, carbon, carbon dioxide, chemical, chemistry, co2, collection, data, dataset, date, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, embryo, erddap, experiment, fertilization, final, final_length, final_sample_age, final_sample_date, growth, growth_rate, hatch, hatch_length, hatch_sample_age, hatch_sample_date, larval, latitude, length, longitude, management, ocean, oceanography, oceans, office, pCO2, preliminary, rate, rel, rel_embryo_survival, rel_larval_survival, replicate, reported, sample, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, site, species, survival, tank, temperature, time, total, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/732818/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/732818
attribute	NC_GLOBAL	Northernmost_Northing	double	41.321526
attribute	NC_GLOBAL	param_mapping	String	{'732818': {'latitude': 'flag - latitude', 'longitude': 'flag - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/732818/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Connecticut
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UConn
attribute	NC_GLOBAL	people_0_person_name	String	Hannes Baumann
attribute	NC_GLOBAL	people_0_person_nid	String	528586
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Connecticut
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UConn - Avery Point
attribute	NC_GLOBAL	people_1_person_name	String	Christopher S. Murray
attribute	NC_GLOBAL	people_1_person_nid	String	742121
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Stony Brook University - SoMAS
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	SUNY-SB SoMAS
attribute	NC_GLOBAL	people_2_person_name	String	Janet Nye
attribute	NC_GLOBAL	people_2_person_nid	String	650186
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	University of Connecticut
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	UConn
attribute	NC_GLOBAL	people_3_person_name	String	Hannes Baumann
attribute	NC_GLOBAL	people_3_person_nid	String	528586
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_4_person_nid	String	51498
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	HYPOA
attribute	NC_GLOBAL	projects_0_acronym	String	HYPOA
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations. This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity. Related references: Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:10.1098/rsbl.2015.0976 Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:10.1002/loe2.10002 Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:10.3354/meps11142
attribute	NC_GLOBAL	projects_0_end_date	String	2018-11
attribute	NC_GLOBAL	projects_0_geolocation	String	Eastern Long Island Sound, CT, USA
attribute	NC_GLOBAL	projects_0_name	String	Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
attribute	NC_GLOBAL	projects_0_project_nid	String	650184
attribute	NC_GLOBAL	projects_0_start_date	String	2015-12
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	40.947376
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	species
attribute	NC_GLOBAL	summary	String	In five individual rearing experiments, wild-caught M. menidia adults were spawned to test offspring sensitivity to factorial combinations of pCO2 (nominal: 400, 2200, 4000, and 6000 \u00b5atm) and temperature (17, 20, 24, and 28 \u00b0C) through measurements of early-life survival and growth. For experiment 1, adults were collected from Poquot Beach (40.947376, -73.10258), and the experiment took place at Stony Brook University\u2019s Flax Pond Marine Laboratory. For experiments 2\u20135, spawning adults were collected from Mumford Cove (41.321526, -72.015247), and experiments were conducted in the Rankin Seawater Facility at University of Connecticut\u2019s Avery Point campus. The experiments quantified two survival and two growth traits for each replicate and CO2 \u00d7 temperature treatment; embryo survival (fertilization to 1 dph), larval survival (1 dph to experiment termination), size (SL) at hatch (1 dph), and growth rate ((SL at end of experiment \u2013 SL 1dph)/number days reared post hatch). These data are published in: Murray, C.S., and Baumann, H. (2018) You Better Repeat It: Complex CO2 \u00d7 Temperature Effects in Atlantic Silverside Offspring Revealed by Serial experimentation. Diversity. doi:10.3390/d10030069.
attribute	NC_GLOBAL	title	String	[CO2 x temperature effects on Menidia menidia offspring] - CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments (Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-73.10258
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	experiment		byte
attribute	experiment	_FillValue	byte	127
attribute	experiment	actual_range	byte	1, 5
attribute	experiment	bcodmo_name	String	exp_id
attribute	experiment	description	String	Experiment number
attribute	experiment	long_name	String	Experiment
attribute	experiment	units	String	unitless
variable	species		String
attribute	species	bcodmo_name	String	species
attribute	species	description	String	Scientific name of the speices (Menidia menidia, the Atlantic silverside)
attribute	species	long_name	String	Species
attribute	species	units	String	unitless
variable	adult_collection_site		String
attribute	adult_collection_site	bcodmo_name	String	site
attribute	adult_collection_site	description	String	Site of the collection of wild adult spawners
attribute	adult_collection_site	long_name	String	Adult Collection Site
attribute	adult_collection_site	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	40.947376, 41.321526
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude of adult collection site
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-73.10258, -72.015247
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude of adult collection site
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	tank		String
attribute	tank	bcodmo_name	String	tank
attribute	tank	description	String	Tank number
attribute	tank	long_name	String	Tank
attribute	tank	units	String	unitless
variable	replicate		byte
attribute	replicate	_FillValue	byte	127
attribute	replicate	actual_range	byte	1, 99
attribute	replicate	bcodmo_name	String	replicate
attribute	replicate	description	String	Within experiment replicate number
attribute	replicate	long_name	String	Replicate
attribute	replicate	units	String	unitless
variable	temperature		float
attribute	temperature	_FillValue	float	NaN
attribute	temperature	actual_range	float	16.9, 28.2
attribute	temperature	bcodmo_name	String	temperature
attribute	temperature	description	String	Average temperature experienced by fish in degrees celsius
attribute	temperature	long_name	String	Temperature
attribute	temperature	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	temperature	units	String	degrees Celsius
variable	pH		float
attribute	pH	_FillValue	float	NaN
attribute	pH	actual_range	float	7.14, 8.24
attribute	pH	bcodmo_name	String	pH
attribute	pH	colorBarMaximum	double	9.0
attribute	pH	colorBarMinimum	double	7.0
attribute	pH	description	String	Average pH level experienced by the fish NBS
attribute	pH	long_name	String	Sea Water Ph Reported On Total Scale
attribute	pH	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/
attribute	pH	units	String	pH NBS
variable	pCO2		short
attribute	pCO2	_FillValue	short	32767
attribute	pCO2	actual_range	short	322, 6195
attribute	pCO2	bcodmo_name	String	pCO2
attribute	pCO2	description	String	Calculated average pCO2 levels in µatm calculated by CO2SYS based on alkalinity, pH, and temperature
attribute	pCO2	long_name	String	P CO2
attribute	pCO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	pCO2	units	String	atm
variable	fertilization_date		String
attribute	fertilization_date	bcodmo_name	String	date
attribute	fertilization_date	description	String	Date offspring were fertilized by stripspawning 20+ adults of each sex; formatted as yyyy/mm/dd
attribute	fertilization_date	long_name	String	Fertilization Date
attribute	fertilization_date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	fertilization_date	source_name	String	fertilization_date
attribute	fertilization_date	time_precision	String	1970-01-01
attribute	fertilization_date	units	String	unitless
variable	hatch_sample_date		String
attribute	hatch_sample_date	bcodmo_name	String	date
attribute	hatch_sample_date	description	String	Date offspring were sampled for hatch survival and measurements; formatted as yyyy/mm/dd
attribute	hatch_sample_date	long_name	String	Hatch Sample Date
attribute	hatch_sample_date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	hatch_sample_date	time_precision	String	1970-01-01
attribute	hatch_sample_date	units	String	unitless
variable	hatch_sample_age		byte
attribute	hatch_sample_age	_FillValue	byte	127
attribute	hatch_sample_age	actual_range	byte	5, 15
attribute	hatch_sample_age	bcodmo_name	String	age
attribute	hatch_sample_age	description	String	Age in days post fertilization (dpf) on the date of hatch sampling
attribute	hatch_sample_age	long_name	String	Hatch Sample Age
attribute	hatch_sample_age	units	String	days
variable	final_sample_date		String
attribute	final_sample_date	bcodmo_name	String	date
attribute	final_sample_date	description	String	Date offspring were sampled for final survival and measurements; formatted as yyyy/mm/dd
attribute	final_sample_date	long_name	String	Final Sample Date
attribute	final_sample_date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	final_sample_date	time_precision	String	1970-01-01
attribute	final_sample_date	units	String	unitless
variable	final_sample_age		byte
attribute	final_sample_age	_FillValue	byte	127
attribute	final_sample_age	actual_range	byte	14, 36
attribute	final_sample_age	bcodmo_name	String	age
attribute	final_sample_age	description	String	Age in days post fertilization (dpf) on the date of final sampling
attribute	final_sample_age	long_name	String	Final Sample Age
attribute	final_sample_age	units	String	days
variable	rel_embryo_survival		float
attribute	rel_embryo_survival	_FillValue	float	NaN
attribute	rel_embryo_survival	actual_range	float	0.39, 1.0
attribute	rel_embryo_survival	bcodmo_name	String	unknown
attribute	rel_embryo_survival	description	String	relative survival of embryos (0-1)
attribute	rel_embryo_survival	long_name	String	Rel Embryo Survival
attribute	rel_embryo_survival	units	String	unitless (fraction)
variable	rel_larval_survival		float
attribute	rel_larval_survival	_FillValue	float	NaN
attribute	rel_larval_survival	actual_range	float	0.03, 1.0
attribute	rel_larval_survival	bcodmo_name	String	unknown
attribute	rel_larval_survival	description	String	relative survival from hatch to experiment termination (0-1)
attribute	rel_larval_survival	long_name	String	Rel Larval Survival
attribute	rel_larval_survival	units	String	unitless (fraction)
variable	hatch_length		float
attribute	hatch_length	_FillValue	float	NaN
attribute	hatch_length	actual_range	float	4.42, 5.74
attribute	hatch_length	bcodmo_name	String	length
attribute	hatch_length	description	String	Average replicate hatch length (mm)
attribute	hatch_length	long_name	String	Hatch Length
attribute	hatch_length	units	String	millimeters (mm)
variable	final_length		float
attribute	final_length	_FillValue	float	NaN
attribute	final_length	actual_range	float	6.95, 10.57
attribute	final_length	bcodmo_name	String	length
attribute	final_length	description	String	Average replicate final length (mm)
attribute	final_length	long_name	String	Final Length
attribute	final_length	units	String	millimeters (mm)
variable	growth_rate		float
attribute	growth_rate	_FillValue	float	NaN
attribute	growth_rate	actual_range	float	0.14, 0.56
attribute	growth_rate	bcodmo_name	String	growth
attribute	growth_rate	description	String	average replicate growth rate (mm d-1) of offspring from hatch to experiment termination
attribute	growth_rate	long_name	String	Growth Rate
attribute	growth_rate	units	String	millimeters per day (mm d-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.