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Dataset Title:  [Proteinaceous material content] - Protein assay of proteinaceous material in
pelagic sediment from the North Atlantic gyre, South Pacific gyre, and Peru
Basin from cruises KN223, KNOX02RR, and ODP leg 201 between 2002 and
2013 (Geochemical controls on organic carbon quantity and quality in the deep
subsurface)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_782742)
Information:  Summary ? | License ? | FGDC | ISO 19115 | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 location (unitless) ?          "North Atlantic"    "South Pacific"
 expedition (unitless) ?          "KN223"    "ODP leg 201"
 site (unitless) ?          "1"    "9"
 date (unitless) ?          "2002-03-20"    "2014-11-24"
 latitude (degrees_north) ?          -38.06167    33.48333
  < slider >
 longitude (degrees_east) ?          -165.64333    -50.62
  < slider >
 depth (Water Depth, m) ?          4455.0    5699.0
  < slider >
 core_type (unitless) ?          "APC"    "piston"
 depth_ (meters (m)) ?          0.02    112.65
 protein (micrograms of protein per milligram of sediment (μg protein/mg sediment)) ?          0.0    3.51
 prot_stdev (micrograms of protein per milligram of sediment (μg protein/mg sediment)) ?          0.0    1.39
 
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File type: (more information)

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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  location {
    String bcodmo_name "site";
    String description "Sample origin";
    String long_name "Location";
    String units "unitless";
  }
  expedition {
    String bcodmo_name "cruise_id";
    String description "Cruise name";
    String long_name "Expedition";
    String units "unitless";
  }
  site {
    String bcodmo_name "site";
    String description "Site number";
    String long_name "Site";
    String units "unitless";
  }
  date {
    String bcodmo_name "date";
    String description "date sample collection in ISO 8601 format yyyy-mm-dd";
    String long_name "Date";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/";
    String source_name "date";
    String time_precision "1970-01-01";
    String units "unitless";
  }
  latitude {
    String _CoordinateAxisType "Lat";
    Float64 _FillValue NaN;
    Float64 actual_range -38.06167, 33.48333;
    String axis "Y";
    String bcodmo_name "latitude";
    Float64 colorBarMaximum 90.0;
    Float64 colorBarMinimum -90.0;
    String description "Latitude";
    String ioos_category "Location";
    String long_name "Latitude";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LATX/";
    String standard_name "latitude";
    String units "degrees_north";
  }
  longitude {
    String _CoordinateAxisType "Lon";
    Float64 _FillValue NaN;
    Float64 actual_range -165.64333, -50.62;
    String axis "X";
    String bcodmo_name "longitude";
    Float64 colorBarMaximum 180.0;
    Float64 colorBarMinimum -180.0;
    String description "Longitude";
    String ioos_category "Location";
    String long_name "Longitude";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LONX/";
    String standard_name "longitude";
    String units "degrees_east";
  }
  depth {
    String _CoordinateAxisType "Height";
    String _CoordinateZisPositive "down";
    Float64 _FillValue NaN;
    Float64 actual_range 4455.0, 5699.0;
    String axis "Z";
    String bcodmo_name "depth";
    String description "Water depth";
    String ioos_category "Location";
    String long_name "Water Depth";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/DEPH/";
    String positive "down";
    String standard_name "depth";
    String units "m";
  }
  core_type {
    String bcodmo_name "sample_type";
    String description "Coring device used";
    String long_name "Core Type";
    String units "unitless";
  }
  depth_ {
    Float32 _FillValue NaN;
    Float32 actual_range 0.02, 112.65;
    String bcodmo_name "meters below seafloor";
    Float64 colorBarMaximum 8000.0;
    Float64 colorBarMinimum -8000.0;
    String colorBarPalette "TopographyDepth";
    String description "Sample depth (meters below seafloor)";
    String long_name "Depth";
    String standard_name "depth";
    String units "meters (m)";
  }
  protein {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 3.51;
    String bcodmo_name "proteins";
    String description "Content of proteinaceous material";
    String long_name "Protein";
    String units "micrograms of protein per milligram of sediment (μg protein/mg sediment)";
  }
  prot_stdev {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 1.39;
    String bcodmo_name "proteins";
    String description "Standard deviation of the content of proteinaceous material (triplicate analyses).";
    String long_name "Prot Stdev";
    String units "micrograms of protein per milligram of sediment (μg protein/mg sediment)";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson";
    String acquisition_description 
"Sediment samples were stored at 4\\u00b0C prior to analysis. Proteinaceous
material was extracted and quantified with Qubit fluorescent reagent (Life
Technologies), following Estes et al. (2016) as modified from Ehrenreich and
Widdel (1994).
 
Assay was quantified using bovine serum albumin standard (Bio-Rad).
 
See Estes el al. 2019, for complete methods.";
    String awards_0_award_nid "554980";
    String awards_0_award_number "OCE-0939564";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward?AWD_ID=0939564";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "David L. Garrison";
    String awards_0_program_manager_nid "50534";
    String cdm_data_type "Other";
    String comment 
"Proteinaceous material content 
  PI(s): Emily Estes 
  Data Version 1: 2020-06-15";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv version 2.3  19 Dec 2019";
    String dataset_current_state "Final and no updates";
    String date_created "2019-11-22T17:52:19Z";
    String date_modified "2020-07-14T19:35:09Z";
    String defaultDataQuery "&amp;time&lt;now";
    String doi "10.26008/1912/bco-dmo.782742.1";
    Float64 Easternmost_Easting -50.62;
    Float64 geospatial_lat_max 33.48333;
    Float64 geospatial_lat_min -38.06167;
    String geospatial_lat_units "degrees_north";
    Float64 geospatial_lon_max -50.62;
    Float64 geospatial_lon_min -165.64333;
    String geospatial_lon_units "degrees_east";
    Float64 geospatial_vertical_max 5699.0;
    Float64 geospatial_vertical_min 4455.0;
    String geospatial_vertical_positive "down";
    String geospatial_vertical_units "m";
    String history 
"2024-11-23T17:18:06Z (local files)
2024-11-23T17:18:06Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_782742.html";
    String infoUrl "https://www.bco-dmo.org/dataset/782742";
    String institution "BCO-DMO";
    String instruments_0_acronym "Piston Corer";
    String instruments_0_dataset_instrument_nid "782808";
    String instruments_0_description "The piston corer is a type of bottom sediment sampling device. A long, heavy tube is plunged into the seafloor to extract samples of mud sediment. A piston corer uses a \"free fall\" of the coring rig to achieve a greater initial force on impact than gravity coring.  A sliding piston inside the core barrel reduces inside wall friction with the sediment and helps to evacuate displaced water from the top of the corer. A piston corer is capable of extracting core samples up to 90 feet in length.";
    String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/51/";
    String instruments_0_instrument_name "Piston Corer";
    String instruments_0_instrument_nid "519";
    String instruments_1_acronym "Gravity Corer";
    String instruments_1_dataset_instrument_nid "782803";
    String instruments_1_description "The gravity corer allows researchers to sample sediment layers at the bottom of lakes or oceans. The coring device is deployed from the ship and gravity carries it to the seafloor. (https://www.whoi.edu/instruments/viewInstrument.do?id=1079).";
    String instruments_1_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/51/";
    String instruments_1_instrument_name "Gravity Corer";
    String instruments_1_instrument_nid "531";
    String instruments_2_acronym "Multi Corer";
    String instruments_2_dataset_instrument_nid "782809";
    String instruments_2_description "The Multi Corer is a benthic coring device used to collect multiple, simultaneous, undisturbed sediment/water samples from the seafloor.  Multiple coring tubes with varying sampling capacity depending on tube dimensions are mounted in a frame designed to sample the deep ocean seafloor. For more information, see Barnett et al. (1984) in Oceanologica Acta, 7, pp. 399-408.";
    String instruments_2_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/51/";
    String instruments_2_instrument_name "Multi Corer";
    String instruments_2_instrument_nid "532";
    String instruments_3_acronym "APC";
    String instruments_3_dataset_instrument_nid "782806";
    String instruments_3_description 
"The JOIDES Resolution's Advanced Piston Corer (APC) is used in soft ooze and sediments. The APC is a hydraulically actuated piston corer designed to recover relatively undisturbed samples from very soft to firm sediments.

More information is available from IODP (PDF).";
    String instruments_3_instrument_name "Advanced Piston Corer";
    String instruments_3_instrument_nid "628015";
    String keywords "bco, bco-dmo, biological, chemical, core, core_type, data, dataset, date, depth, deviation, dmo, erddap, expedition, latitude, longitude, management, oceanography, office, preliminary, prot, prot_stdev, protein, site, standard, standard deviation, stdev, time, type, water, water_depth";
    String license "https://www.bco-dmo.org/dataset/782742/license";
    String metadata_source "https://www.bco-dmo.org/api/dataset/782742";
    Float64 Northernmost_Northing 33.48333;
    String param_mapping "{'782742': {'latitude': 'flag - latitude', 'water_depth': 'master - depth', 'longitude': 'flag - longitude'}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/782742/parameters";
    String people_0_affiliation "Texas A&M University";
    String people_0_affiliation_acronym "TAMU";
    String people_0_person_name "Emily Estes";
    String people_0_person_nid "779219";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "Woods Hole Oceanographic Institution";
    String people_1_affiliation_acronym "WHOI";
    String people_1_person_name "Colleen Hansel";
    String people_1_person_nid "756932";
    String people_1_role "Co-Principal Investigator";
    String people_1_role_type "originator";
    String people_2_affiliation "Woods Hole Oceanographic Institution";
    String people_2_affiliation_acronym "WHOI BCO-DMO";
    String people_2_person_name "Amber D. York";
    String people_2_person_nid "643627";
    String people_2_role "BCO-DMO Data Manager";
    String people_2_role_type "related";
    String project "Org C Sed II,Org C Sed I";
    String projects_0_acronym "Org C Sed II";
    String projects_0_description 
"Abstract from the C-DEBI project page:
Sediment underlying ocean gyres receives minimal input of fresh organic matter yet sustains a small but active heterotrophic microbial community. The concentration and composition of the organic carbon (OC) available to this deep biosphere however is unknown. We analyzed the content and composition of OC in pelagic sediment in order to identify mechanism(s) that dictate the balance between OC preservation and utilization by microorganisms. Sediment cores from the North Atlantic gyre (KN223), South Pacific Gyre (Knox02-RR), and Peru Basin (IODP site 1231) allowed for a global comparison and a test of how sediment lithology and redox state affect OC preservation. OC was present in low concentrations in all samples (0.01—0.61%), at depths up to 112 meters below seafloor and estimated sediment ages of up to 50 million years. Synchrotron-based near edge X-ray absorption fine structure (NEXAFS) spectroscopy was conducted on over 100 samples, one of the first applications of NEXAFS to sedimentary environments. NEXAFS revealed an OC reservoir dominated by amide and carboxylic functionalities in a scaffolding of O-alkyl and aliphatic carbons. Detection of extractable, extracellular proteins supports this composition and suggests that sedimentary OC is protein-derived. This composition was common across all sites and depths, implicating physical rather than chemical mechanisms in OC preservation on long timescales. This study thereby points to physical access rather than energy or metabolic potential as a key constraint on subsurface heterotrophic life.";
    String projects_0_end_date "2017-07";
    String projects_0_geolocation "North Atlantic gyre, South Pacific gyre";
    String projects_0_name "Geochemical controls on organic carbon quantity and quality in the deep subsurface";
    String projects_0_project_nid "779216";
    String projects_0_project_website "https://www.darkenergybiosphere.org/award/3d-spatial-mapping-of-the-energetic-return-of-1000-metabolisms-within-the-compositional-variation-of-oceanic-crusts-near-mid-ocean-ridges/";
    String projects_0_start_date "2016-06";
    String projects_1_acronym "Org C Sed I";
    String projects_1_description 
"Abstract from the C-DEBI project page:
Minerals have recently been identified as a primary host for organic carbon (OC) within marine sediments. This strong physical and chemical carbon-mineral association is believed to reduce, and in some cases completely eliminate, the bioavailablilty of this carbon for microbial life. The paucity of information regarding the nature of this carbon-mineral association and the composition of the hosted carbon, however, precludes our ability to predict the ultimate fate of this OC and its involvement in deep subsurface life. Here, we addressed this knowledge gap by using a suite of bulk and spatially-resolved geochemical and mineralogical techniques to characterize OC-mineral associations within the deep subsurface. We characterized sediment samples collected on the 2014 North Atlantic long coring expedition (KN223) in the western subtropical North Atlantic that included three geochemically distinct long cores to a depth of 24-30 m and spanned OC-limited oxic to anoxic sediments. We find measurable and relevant OC concentrations throughout the sediment cores, that decreases linearly over ~25 meters burial depth, from ~0.15 to 0.075 mol OC/kg solid. OC within the sediments is compositionally complex on both a macro- and micro-scale, spanning a gradient of lability even at depth. Proteins are observed throughout the sediment depth profiles, where they appear to constitute a substantial fraction of the TOC. Correspondingly, a low C:N ratio is observed, consistent with proteinaceous carbon within the sediments. In sum, these findings point to a substantial mineral-hosted OC reservoir within the deep subsurface that may fuel the deep biosphere and select for protein-based heterotrophy.";
    String projects_1_end_date "2016-03";
    String projects_1_geolocation "North Atlantic gyre, South Pacific gyre";
    String projects_1_name "Elucidating the extent and composition of mineral-hosted carbon in the deep biosphere";
    String projects_1_project_nid "779223";
    String projects_1_project_website "https://www.darkenergybiosphere.org/award/elucidating-the-extent-and-composition-of-mineral-hosted-carbon-in-the-deep-biosphere/";
    String projects_1_start_date "2015-04";
    String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
    String publisher_type "institution";
    String sourceUrl "(local files)";
    Float64 Southernmost_Northing -38.06167;
    String standard_name_vocabulary "CF Standard Name Table v55";
    String summary "Protein assay of proteinaceous material in pelagic sediment from the North Atlantic gyre, South Pacific gyre, and Peru Basin from cruises KN223 (R/V Knorr), KNOX02RR (R/V Roger Revelle), and ODP leg 201 between 2002 and 2013.  Sediment samples were taken with gravity corers, multi corers, piston and advanced piston corers (APC).";
    String title "[Proteinaceous material content] - Protein assay of proteinaceous material in pelagic sediment from the North Atlantic gyre, South Pacific gyre, and Peru Basin from cruises KN223, KNOX02RR, and ODP leg 201 between 2002 and 2013 (Geochemical controls on organic carbon quantity and quality in the deep subsurface)";
    String version "1";
    Float64 Westernmost_Easting -165.64333;
    String xml_source "osprey2erddap.update_xml() v1.5";
  }
}

 

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