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     data   graph     files  public [Proteinaceous material content] - Protein assay of proteinaceous material in pelagic
sediment from the North Atlantic gyre, South Pacific gyre, and Peru Basin from cruises KN223,
KNOX02RR, and ODP leg 201 between 2002 and 2013 (Geochemical controls on organic carbon
quantity and quality in the deep subsurface)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Sediment samples were stored at 4\u00b0C prior to analysis. Proteinaceous
material was extracted and quantified with Qubit fluorescent reagent (Life
Technologies), following Estes et al. (2016) as modified from Ehrenreich and
Widdel (1994).

Assay was quantified using bovine serum albumin standard (Bio-Rad).

See Estes el al. 2019, for complete methods.
attribute NC_GLOBAL awards_0_award_nid String 554980
attribute NC_GLOBAL awards_0_award_number String OCE-0939564
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=0939564 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Proteinaceous material content
PI(s): Emily Estes
Data Version 1: 2020-06-15
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-11-22T17:52:19Z
attribute NC_GLOBAL date_modified String 2020-07-14T19:35:09Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.782742.1
attribute NC_GLOBAL Easternmost_Easting double -50.62
attribute NC_GLOBAL geospatial_lat_max double 33.48333
attribute NC_GLOBAL geospatial_lat_min double -38.06167
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -50.62
attribute NC_GLOBAL geospatial_lon_min double -165.64333
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 5699.0
attribute NC_GLOBAL geospatial_vertical_min double 4455.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/782742 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Piston Corer
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 782808
attribute NC_GLOBAL instruments_0_description String The piston corer is a type of bottom sediment sampling device. A long, heavy tube is plunged into the seafloor to extract samples of mud sediment. A piston corer uses a "free fall" of the coring rig to achieve a greater initial force on impact than gravity coring. A sliding piston inside the core barrel reduces inside wall friction with the sediment and helps to evacuate displaced water from the top of the corer. A piston corer is capable of extracting core samples up to 90 feet in length.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/51/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Piston Corer
attribute NC_GLOBAL instruments_0_instrument_nid String 519
attribute NC_GLOBAL instruments_1_acronym String Gravity Corer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 782803
attribute NC_GLOBAL instruments_1_description String The gravity corer allows researchers to sample sediment layers at the bottom of lakes or oceans. The coring device is deployed from the ship and gravity carries it to the seafloor. (https://www.whoi.edu/instruments/viewInstrument.do?id=1079).
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/51/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Gravity Corer
attribute NC_GLOBAL instruments_1_instrument_nid String 531
attribute NC_GLOBAL instruments_2_acronym String Multi Corer
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 782809
attribute NC_GLOBAL instruments_2_description String The Multi Corer is a benthic coring device used to collect multiple, simultaneous, undisturbed sediment/water samples from the seafloor. Multiple coring tubes with varying sampling capacity depending on tube dimensions are mounted in a frame designed to sample the deep ocean seafloor. For more information, see Barnett et al. (1984) in Oceanologica Acta, 7, pp. 399-408.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/51/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Multi Corer
attribute NC_GLOBAL instruments_2_instrument_nid String 532
attribute NC_GLOBAL instruments_3_acronym String APC
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 782806
attribute NC_GLOBAL instruments_3_description String The JOIDES Resolution's Advanced Piston Corer (APC) is used in soft ooze and sediments. The APC is a hydraulically actuated piston corer designed to recover relatively undisturbed samples from very soft to firm sediments.

More information is available from IODP (PDF).
attribute NC_GLOBAL instruments_3_instrument_name String Advanced Piston Corer
attribute NC_GLOBAL instruments_3_instrument_nid String 628015
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, core, core_type, data, dataset, date, depth, deviation, dmo, erddap, expedition, latitude, longitude, management, oceanography, office, preliminary, prot, prot_stdev, protein, site, standard, standard deviation, stdev, time, type, water, water_depth
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/782742/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/782742 (external link)
attribute NC_GLOBAL Northernmost_Northing double 33.48333
attribute NC_GLOBAL param_mapping String {'782742': {'latitude': 'flag - latitude', 'water_depth': 'master - depth', 'longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/782742/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Texas A&M University
attribute NC_GLOBAL people_0_affiliation_acronym String TAMU
attribute NC_GLOBAL people_0_person_name String Emily Estes
attribute NC_GLOBAL people_0_person_nid String 779219
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI
attribute NC_GLOBAL people_1_person_name String Colleen Hansel
attribute NC_GLOBAL people_1_person_nid String 756932
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Amber D. York
attribute NC_GLOBAL people_2_person_nid String 643627
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Org C Sed II,Org C Sed I
attribute NC_GLOBAL projects_0_acronym String Org C Sed II
attribute NC_GLOBAL projects_0_description String Abstract from the C-DEBI project page:
Sediment underlying ocean gyres receives minimal input of fresh organic matter yet sustains a small but active heterotrophic microbial community. The concentration and composition of the organic carbon (OC) available to this deep biosphere however is unknown. We analyzed the content and composition of OC in pelagic sediment in order to identify mechanism(s) that dictate the balance between OC preservation and utilization by microorganisms. Sediment cores from the North Atlantic gyre (KN223), South Pacific Gyre (Knox02-RR), and Peru Basin (IODP site 1231) allowed for a global comparison and a test of how sediment lithology and redox state affect OC preservation. OC was present in low concentrations in all samples (0.01—0.61%), at depths up to 112 meters below seafloor and estimated sediment ages of up to 50 million years. Synchrotron-based near edge X-ray absorption fine structure (NEXAFS) spectroscopy was conducted on over 100 samples, one of the first applications of NEXAFS to sedimentary environments. NEXAFS revealed an OC reservoir dominated by amide and carboxylic functionalities in a scaffolding of O-alkyl and aliphatic carbons. Detection of extractable, extracellular proteins supports this composition and suggests that sedimentary OC is protein-derived. This composition was common across all sites and depths, implicating physical rather than chemical mechanisms in OC preservation on long timescales. This study thereby points to physical access rather than energy or metabolic potential as a key constraint on subsurface heterotrophic life.
attribute NC_GLOBAL projects_0_end_date String 2017-07
attribute NC_GLOBAL projects_0_geolocation String North Atlantic gyre, South Pacific gyre
attribute NC_GLOBAL projects_0_name String Geochemical controls on organic carbon quantity and quality in the deep subsurface
attribute NC_GLOBAL projects_0_project_nid String 779216
attribute NC_GLOBAL projects_0_project_website String https://www.darkenergybiosphere.org/award/3d-spatial-mapping-of-the-energetic-return-of-1000-metabolisms-within-the-compositional-variation-of-oceanic-crusts-near-mid-ocean-ridges/ (external link)
attribute NC_GLOBAL projects_0_start_date String 2016-06
attribute NC_GLOBAL projects_1_acronym String Org C Sed I
attribute NC_GLOBAL projects_1_description String Abstract from the C-DEBI project page:
Minerals have recently been identified as a primary host for organic carbon (OC) within marine sediments. This strong physical and chemical carbon-mineral association is believed to reduce, and in some cases completely eliminate, the bioavailablilty of this carbon for microbial life. The paucity of information regarding the nature of this carbon-mineral association and the composition of the hosted carbon, however, precludes our ability to predict the ultimate fate of this OC and its involvement in deep subsurface life. Here, we addressed this knowledge gap by using a suite of bulk and spatially-resolved geochemical and mineralogical techniques to characterize OC-mineral associations within the deep subsurface. We characterized sediment samples collected on the 2014 North Atlantic long coring expedition (KN223) in the western subtropical North Atlantic that included three geochemically distinct long cores to a depth of 24-30 m and spanned OC-limited oxic to anoxic sediments. We find measurable and relevant OC concentrations throughout the sediment cores, that decreases linearly over ~25 meters burial depth, from ~0.15 to 0.075 mol OC/kg solid. OC within the sediments is compositionally complex on both a macro- and micro-scale, spanning a gradient of lability even at depth. Proteins are observed throughout the sediment depth profiles, where they appear to constitute a substantial fraction of the TOC. Correspondingly, a low C:N ratio is observed, consistent with proteinaceous carbon within the sediments. In sum, these findings point to a substantial mineral-hosted OC reservoir within the deep subsurface that may fuel the deep biosphere and select for protein-based heterotrophy.
attribute NC_GLOBAL projects_1_end_date String 2016-03
attribute NC_GLOBAL projects_1_geolocation String North Atlantic gyre, South Pacific gyre
attribute NC_GLOBAL projects_1_name String Elucidating the extent and composition of mineral-hosted carbon in the deep biosphere
attribute NC_GLOBAL projects_1_project_nid String 779223
attribute NC_GLOBAL projects_1_project_website String https://www.darkenergybiosphere.org/award/elucidating-the-extent-and-composition-of-mineral-hosted-carbon-in-the-deep-biosphere/ (external link)
attribute NC_GLOBAL projects_1_start_date String 2015-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -38.06167
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Protein assay of proteinaceous material in pelagic sediment from the North Atlantic gyre, South Pacific gyre, and Peru Basin from cruises KN223 (R/V Knorr), KNOX02RR (R/V Roger Revelle), and ODP leg 201 between 2002 and 2013. Sediment samples were taken with gravity corers, multi corers, piston and advanced piston corers (APC).
attribute NC_GLOBAL title String [Proteinaceous material content] - Protein assay of proteinaceous material in pelagic sediment from the North Atlantic gyre, South Pacific gyre, and Peru Basin from cruises KN223, KNOX02RR, and ODP leg 201 between 2002 and 2013 (Geochemical controls on organic carbon quantity and quality in the deep subsurface)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -165.64333
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable location   String  
attribute location bcodmo_name String site
attribute location description String Sample origin
attribute location long_name String Location
attribute location units String unitless
variable expedition   String  
attribute expedition bcodmo_name String cruise_id
attribute expedition description String Cruise name
attribute expedition long_name String Expedition
attribute expedition units String unitless
variable site   String  
attribute site bcodmo_name String site
attribute site description String Site number
attribute site long_name String Site
attribute site units String unitless
variable date   String  
attribute date bcodmo_name String date
attribute date description String date sample collection in ISO 8601 format yyyy-mm-dd
attribute date long_name String Date
attribute date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -38.06167, 33.48333
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -165.64333, -50.62
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 4455.0, 5699.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth description String Water depth
attribute depth ioos_category String Location
attribute depth long_name String Water Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable core_type   String  
attribute core_type bcodmo_name String sample_type
attribute core_type description String Coring device used
attribute core_type long_name String Core Type
attribute core_type units String unitless
variable depth_   float  
attribute depth_ _FillValue float NaN
attribute depth_ actual_range float 0.02, 112.65
attribute depth_ bcodmo_name String meters below seafloor
attribute depth_ colorBarMaximum double 8000.0
attribute depth_ colorBarMinimum double -8000.0
attribute depth_ colorBarPalette String TopographyDepth
attribute depth_ description String Sample depth (meters below seafloor)
attribute depth_ long_name String Depth
attribute depth_ standard_name String depth
attribute depth_ units String meters (m)
variable protein   float  
attribute protein _FillValue float NaN
attribute protein actual_range float 0.0, 3.51
attribute protein bcodmo_name String proteins
attribute protein description String Content of proteinaceous material
attribute protein long_name String Protein
attribute protein units String micrograms of protein per milligram of sediment (μg protein/mg sediment)
variable prot_stdev   float  
attribute prot_stdev _FillValue float NaN
attribute prot_stdev actual_range float 0.0, 1.39
attribute prot_stdev bcodmo_name String proteins
attribute prot_stdev description String Standard deviation of the content of proteinaceous material (triplicate analyses).
attribute prot_stdev long_name String Prot Stdev
attribute prot_stdev units String micrograms of protein per milligram of sediment (μg protein/mg sediment)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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