Accessing BCO-DMO data
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BCO-DMO ERDDAP
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| Dataset Title: | [Synechococcus DOP Hydrolysis Experiment - cell counts and IVF] - In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018- 2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) 
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| Institution: | BCO-DMO (Dataset ID: bcodmo_dataset_929471_v1) |
| Information: | Summary
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Attributes {
s {
synechococcus_strain {
String long_name "Synechococcus_strain";
String units "unitless";
}
time_day {
Int32 actual_range 0, 24;
String long_name "Time_day";
String units "days";
}
media_and_phosphate_level {
String long_name "Media_and_phosphate_level";
String units "unitless";
}
in_vivo_fluor_trip1 {
Float32 actual_range 16.5, 589.5;
String long_name "In_vivo_fluor_trip1";
String units "relative fluorescence units (RFU)";
}
in_vivo_fluor_trip2 {
Float32 actual_range 16.5, 565.5;
String long_name "In_vivo_fluor_trip2";
String units "relative fluorescence units (RFU)";
}
in_vivo_fluor_trip3 {
Float32 actual_range 16.5, 617.5;
String long_name "In_vivo_fluor_trip3";
String units "relative fluorescence units (RFU)";
}
cell_counts_trip1 {
Int32 actual_range 22435, 309927090;
String long_name "Cell_counts_trip1";
String units "cells per milliliter (cells mL-1)";
}
cell_counts_trip2 {
Int32 actual_range 44869, 409953052;
String long_name "Cell_counts_trip2";
String units "cells per milliliter (cells mL-1)";
}
cell_counts_trip3 {
Int32 actual_range 33652, 340458870;
String long_name "Cell_counts_trip3";
String units "cells per milliliter (cells mL-1)";
}
}
NC_GLOBAL {
String cdm_data_type "Other";
String Conventions "COARDS, CF-1.6, ACDD-1.3";
String creator_email "info@bco-dmo.org";
String creator_name "BCO-DMO";
String creator_url "https://www.bco-dmo.org/";
String doi "10.26008/1912/bco-dmo.929471.1";
String history
"2024-09-27T19:25:22Z (local files)
2024-09-27T19:25:22Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929471_v1.html";
String infoUrl "https://www.bco-dmo.org/dataset/929471";
String institution "BCO-DMO";
String license
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
String sourceUrl "(local files)";
String summary
"Marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory culture in vivo fluorescence and flow cytometry cell counts following growth in high and low phosphate media, as part of the DOP hydrolysis experiments in a study of \"Dissolved organic phosphorus bond-class utilization by Synechococcus\" (Waggoner et al. 2024).
Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.";
String title "[Synechococcus DOP Hydrolysis Experiment - cell counts and IVF] - In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)";
}
}
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Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names,
followed by a collection of
constraints (e.g., variable<value),
each preceded by '&' (which is interpreted as "AND").
For details, see the tabledap Documentation.