ERDDAP > info > bcodmo_dataset_929471_v1

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Synechococcus DOP Hydrolysis Experiment - cell counts and IVF] - In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018- 2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)		M	background			BCO-DMO	bcodmo_dataset_929471_v1

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.929471.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/929471
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	license	String	The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	summary	String	Marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory culture in vivo fluorescence and flow cytometry cell counts following growth in high and low phosphate media, as part of the DOP hydrolysis experiments in a study of "Dissolved organic phosphorus bond-class utilization by Synechococcus" (Waggoner et al. 2024). Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
attribute	NC_GLOBAL	title	String	[Synechococcus DOP Hydrolysis Experiment - cell counts and IVF] - In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)
variable	synechococcus_strain		String
attribute	synechococcus_strain	long_name	String	Synechococcus_strain
attribute	synechococcus_strain	units	String	unitless
variable	time_day		int
attribute	time_day	actual_range	int	0, 24
attribute	time_day	long_name	String	Time_day
attribute	time_day	units	String	days
variable	media_and_phosphate_level		String
attribute	media_and_phosphate_level	long_name	String	Media_and_phosphate_level
attribute	media_and_phosphate_level	units	String	unitless
variable	in_vivo_fluor_trip1		float
attribute	in_vivo_fluor_trip1	actual_range	float	16.5, 589.5
attribute	in_vivo_fluor_trip1	long_name	String	In_vivo_fluor_trip1
attribute	in_vivo_fluor_trip1	units	String	relative fluorescence units (RFU)
variable	in_vivo_fluor_trip2		float
attribute	in_vivo_fluor_trip2	actual_range	float	16.5, 565.5
attribute	in_vivo_fluor_trip2	long_name	String	In_vivo_fluor_trip2
attribute	in_vivo_fluor_trip2	units	String	relative fluorescence units (RFU)
variable	in_vivo_fluor_trip3		float
attribute	in_vivo_fluor_trip3	actual_range	float	16.5, 617.5
attribute	in_vivo_fluor_trip3	long_name	String	In_vivo_fluor_trip3
attribute	in_vivo_fluor_trip3	units	String	relative fluorescence units (RFU)
variable	cell_counts_trip1		int
attribute	cell_counts_trip1	actual_range	int	22435, 309927090
attribute	cell_counts_trip1	long_name	String	Cell_counts_trip1
attribute	cell_counts_trip1	units	String	cells per milliliter (cells mL-1)
variable	cell_counts_trip2		int
attribute	cell_counts_trip2	actual_range	int	44869, 409953052
attribute	cell_counts_trip2	long_name	String	Cell_counts_trip2
attribute	cell_counts_trip2	units	String	cells per milliliter (cells mL-1)
variable	cell_counts_trip3		int
attribute	cell_counts_trip3	actual_range	int	33652, 340458870
attribute	cell_counts_trip3	long_name	String	Cell_counts_trip3
attribute	cell_counts_trip3	units	String	cells per milliliter (cells mL-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.