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Dataset Title:  [Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-
2RC)] - Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and
Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb
2022 to Oct 2023 (Collaborative Research: VIDA Seagrass: Viral Infection
Dynamics Among Seagrass)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_931469_v1)
Information:  Summary ? | License ? | FGDC | ISO 19115 | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 sample (unitless) ?          "101"    "S3T8 Tt 2023"
 region (unitless) ?          "FL, USA"    "FL, USA"
 site (unitless) ?          "Bush Key"    "Terra Ceia Aquatic..."
 date (unitless) ?          "2022-02-04"    "2023-10-03"
 latitude (degrees_north) ?          24.56098    29.99911
  < slider >
 longitude (degrees_east) ?          -84.35056    -81.79929
  < slider >
 sample_host (unitless) ?          "Thalassia testudinum"    "Thalassia testudinum"
 Aphia_id (unitless) ?          "3744720"    "3744720"
 Genbank_accession (unitless) ?          "OR827692"    "PP430571"
 
Server-side Functions ?
 distinct() ?
? ("Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.")

File type: (more information)

(Documentation / Bypass this form ? )
 
(Please be patient. It may take a while to get the data.)


 

The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  sample {
    String long_name "Sample";
    String units "unitless";
  }
  region {
    String long_name "Region";
    String units "unitless";
  }
  site {
    String long_name "Site";
    String units "unitless";
  }
  date {
    String long_name "Date";
    String units "unitless";
  }
  latitude {
    String _CoordinateAxisType "Lat";
    Float32 actual_range 24.56098, 29.99911;
    String axis "Y";
    String ioos_category "Location";
    String long_name "Latitude";
    String standard_name "latitude";
    String units "degrees_north";
  }
  longitude {
    String _CoordinateAxisType "Lon";
    Float32 actual_range -84.35056, -81.79929;
    String axis "X";
    String ioos_category "Location";
    String long_name "Longitude";
    String standard_name "longitude";
    String units "degrees_east";
  }
  sample_host {
    String long_name "Sample_host";
    String units "unitless";
  }
  Aphia_id {
    String long_name "Aphia_id";
    String units "unitless";
  }
  Genbank_accession {
    String long_name "Genbank_accession";
    String units "unitless";
  }
 }
  NC_GLOBAL {
    String cdm_data_type "Other";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_url "https://www.bco-dmo.org/";
    String doi "10.26008/1912/bco-dmo.931469.1";
    Float64 Easternmost_Easting -81.79929;
    Float64 geospatial_lat_max 29.99911;
    Float64 geospatial_lat_min 24.56098;
    String geospatial_lat_units "degrees_north";
    Float64 geospatial_lon_max -81.79929;
    Float64 geospatial_lon_min -84.35056;
    String geospatial_lon_units "degrees_east";
    String history 
"2024-12-25T07:41:38Z (local files)
2024-12-25T07:41:38Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_931469_v1.html";
    String infoUrl "https://www.bco-dmo.org/dataset/931469";
    String institution "BCO-DMO";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    Float64 Northernmost_Northing 29.99911;
    String sourceUrl "(local files)";
    Float64 Southernmost_Northing 24.56098;
    String summary 
"This dataset includes Sanger sequence references for 40 Thalassia testudinum samples collected during systematic seagrass surveys from multiple Florida sites, including Terra Ceia Aquatic Preserve, Tampa Bay seagrass sites S1T5 and S3T8 (Lassing Park), Panacea located in the Florida Panhandle, and Florida Keys sites including Bush Key, Garden Key, Marquesas Key, and Key West.
We investigated potexvirus distribution in seagrasses using a degenerate reverse transcription polymerase chain reaction (RT-PCR) assay originally designed to capture potexvirus diversity in terrestrial plants. The assay, which implements Potex-5 and Potex-2RC primers, successfully amplified a 584 nt RNA-dependent RNA polymerase (RdRp) fragment from TVX-infected seagrasses. Following validation, we screened 74 opportunistically collected, apparently healthy seagrass samples for potexviruses using this RT-PCR assay.
Potexvirus PCR products were successfully generated from Thalassia testudinum samples. Sequences from these products are deposited in NCBI GenBank under the accession numbers OR827692-OR827705, OR854648, OR863396, OR879052-OR879056, and PP430548-PP430571.";
    String title "[Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC)] - Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023 (Collaborative Research: VIDA Seagrass: Viral Infection Dynamics Among Seagrass)";
    Float64 Westernmost_Easting -84.35056;
  }
}

 

Using tabledap to Request Data and Graphs from Tabular Datasets

tabledap lets you request a data subset, a graph, or a map from a tabular dataset (for example, buoy data), via a specially formed URL. tabledap uses the OPeNDAP (external link) Data Access Protocol (DAP) (external link) and its selection constraints (external link).

The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.

Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names, followed by a collection of constraints (e.g., variable<value), each preceded by '&' (which is interpreted as "AND").

For details, see the tabledap Documentation.


 
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