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Dataset Title:  [PUPCYCLE 2019 incubation experiments] - Incubation experiments to assess the
physiological and molecular response of subsurface phytoplankton communities to
a simulation of the Upwelling Conveyor Belt Cycle (UCBC) conducted in summer
2019 onboard the R/V Oceanus (OC1905b) (CAREER: An integrated molecular and
physiological approach to examining the dynamics of upwelled phytoplankton in
current and changing oceans)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_949788_v1)
Information:  Summary ? | License ? | FGDC | ISO 19115 | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 Experiment (unitless) ?          "Narrow shelf_Fe st..."    "Wide shelf_seed po..."
 Time_Point (unitless) ?          "T0"    "T2"
 Hours (hours) ?          0    120
 Treatment (unitless) ?          "Ctrl-A"    "SWDW"
 Replicate (unitless) ?          "A"    "C"
 Sample_Name (unitless) ?          "Q1"    "Q97"
 Date (unitless) ?          "2019-05-27"    "2019-06-04"
 latitude (degrees_north) ?          35.92353    41.01471
  < slider >
 longitude (degrees_east) ?          -124.4163    -121.5429
  < slider >
 NO3 (micromolar (uM)) ?          -0.023    22.1
 PO4 (micromolar (uM)) ?          0.194    2.33
 SiOH4 (micromolar (uM)) ?          0.0    34.5
 chl_a_gt_5um (micrograms per liter (ug/L)) ?          0.0138918    14.84532
 chl_a_GFF (micrograms per liter (ug/L)) ?          -0.0212898    1.55358
 chl_a_Total (micrograms per liter (ug/L)) ?          0.0166866    11.85324
 Fv_Fm (unitless) ?          0.279    0.611
 Sigma (unitless) ?          248    1005
 PON_gt_5um (micromoles per liter (umol/L)) ?          0.1518217    18.35824
 PON_GFF (micromoles per liter (umol/L)) ?          0.2347731    4.86793
 PON_Total (micromoles per liter (umol/L)) ?          0.3969211    20.2548
 POC_gt_5um (micromoles per liter (umol/L)) ?          -0.4438948    185.2285
 POC_GFF (micromoles per liter (umol/L)) ?          -0.0020471    28.16849
 POC_Total (micromoles per liter (umol/L)) ?          0.0690021    196.2137
 N_Uptake_gt_5um (micromoles per liter per day (umol/L/day)) ?          0.0176493    9.650486
 N_Uptake_GFF (micromoles per liter per day (umol/L/day)) ?          0.0162029    1.384334
 N_Uptake_Total (micromoles per liter per day (umol/L/day)) ?          0.0392619    10.06306
 Biomass_normalized_N_Uptake_gt_5um (per day) ?          0.0505332    0.900757
 Biomass_normalized_N_Uptake_GFF (per day) ?          0.0426665    0.4607246
 Biomass_normalized_N_Uptake_Total (per day) ?          0.1179996    1.292037
 C_Uptake_5um (micromoles per liter per day (umol/L/day)) ?          -0.0439365    226.1147
 C_Uptake_GFF (micromoles per liter per day (umol/L/day)) ?          -1.76E-4    26.58769
 C_Uptake_Total (micromoles per liter per day (umol/L/day)) ?          -0.0045826    231.3316
 Biomass_normalized_C_Uptake_gt_5um (per day) ?          0.0166    1.5902
 Biomass_normalized_C_Uptake_GFF (per day) ?          0.0128    0.9587
 Biomass_normalized_C_Uptake_Total (per day) ?          0.0293456    2.308585
 Particulate_DA (micrograms per liter (ug/L)) ?          0.027    12.402
 Dissolved_DA (micrograms per liter (ug/L)) ?          0.0    12.91
 Dissolved_Y (picomoles per kilogram (pmol/kg)) ?          101.0231    194.4722
 Dissolved_Cd (picomoles per kilogram (pmol/kg)) ?          100.3228    796.1678
 Dissolved_La (picomoles per kilogram (pmol/kg)) ?          15.88603    51.45339
 Dissolved_Pb (picomoles per kilogram (pmol/kg)) ?          16.29469    82.12563
 Dissolved_Ce (picomoles per kilogram (pmol/kg)) ?          5.275867    38.30345
 Dissolved_Sc (picomoles per kilogram (pmol/kg)) ?          0.791731    13.88508
 Dissolved_Mn (nanomoles per kilogram (nmol/kg)) ?          0.918466    10.77443
 Dissolved_Co (picomoles per kilogram (pmol/kg)) ?          62.46153    563.6546
 Dissolved_Ni (nanomoles per kilogram (nmol/kg)) ?          3.041688    10.87644
 Dissolved_Cu (nanomoles per kilogram (nmol/kg)) ?          0.7565952    8.093715
 Dissolved_Zn (nanomoles per kilogram (nmol/kg)) ?          0.1360039    5.341446
 Dissolved_Fe (nanomoles per kilogram (nmol/kg)) ?          0.055    6.038
 Particulate_Fe_pFe_leached_gt_5um (nanomoles per kilogram (nmol/kg)) ?          0.181    16.306
 Fe_N_ratio (unitless) ?          0.0218    4.6151
 Fe_uptake_lt_5um (picomoles Fe per liter per hour (pmol Fe/L/h)) ?          0.0    0.897
 Fe_uptake_gt_5um (picomoles Fe per liter per hour (pmol Fe/L/h)) ?          0.021    0.687
 Fe_uptake_lt_5um_pmol_Fe_ug_Chla_h (picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h)) ?          0.288    26.573
 Fe_uptake_gt_5um_pmol_Fe_ug_Chla_h (picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h)) ?          0.005    22.598
 Fe_uptake_lt_5um_pmol_Fe_umol_POC_h (picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h)) ?          0.019    0.648
 Fe_uptake_gt_5um_pmol_Fe_umol_POC_h (picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h)) ?          0.001    0.697
 
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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  Experiment {
    String long_name "Experiment";
    String units "unitless";
  }
  Time_Point {
    String long_name "Time_point";
    String units "unitless";
  }
  Hours {
    Int32 actual_range 0, 120;
    String long_name "Hours";
    String units "hours";
  }
  Treatment {
    String long_name "Treatment";
    String units "unitless";
  }
  Replicate {
    String long_name "Replicate";
    String units "unitless";
  }
  Sample_Name {
    String long_name "Sample_name";
    String units "unitless";
  }
  Date {
    String long_name "Date";
    String units "unitless";
  }
  latitude {
    String _CoordinateAxisType "Lat";
    Float32 actual_range 35.92353, 41.01471;
    String axis "Y";
    String ioos_category "Location";
    String long_name "Latitude";
    String standard_name "latitude";
    String units "degrees_north";
  }
  longitude {
    String _CoordinateAxisType "Lon";
    Float32 actual_range -124.4163, -121.5429;
    String axis "X";
    String ioos_category "Location";
    String long_name "Longitude";
    String standard_name "longitude";
    String units "degrees_east";
  }
  NO3 {
    Float32 actual_range -0.023, 22.1;
    String long_name "No3";
    String units "micromolar (uM)";
  }
  PO4 {
    Float32 actual_range 0.194, 2.33;
    String long_name "Po4";
    String units "micromolar (uM)";
  }
  SiOH4 {
    Float32 actual_range 0.0, 34.5;
    String long_name "Sioh4";
    String units "micromolar (uM)";
  }
  chl_a_gt_5um {
    Float32 actual_range 0.0138918, 14.84532;
    String long_name "Chl_a_gt_5um";
    String units "micrograms per liter (ug/L)";
  }
  chl_a_GFF {
    Float32 actual_range -0.0212898, 1.55358;
    String long_name "Chl_a_gff";
    String units "micrograms per liter (ug/L)";
  }
  chl_a_Total {
    Float32 actual_range 0.0166866, 11.85324;
    String long_name "Chl_a_total";
    String units "micrograms per liter (ug/L)";
  }
  Fv_Fm {
    Float32 actual_range 0.279, 0.611;
    String long_name "Fv_fm";
    String units "unitless";
  }
  Sigma {
    Int32 actual_range 248, 1005;
    String long_name "Sigma";
    String units "unitless";
  }
  PON_gt_5um {
    Float32 actual_range 0.1518217, 18.35824;
    String long_name "Pon_gt_5um";
    String units "micromoles per liter (umol/L)";
  }
  PON_GFF {
    Float32 actual_range 0.2347731, 4.86793;
    String long_name "Pon_gff";
    String units "micromoles per liter (umol/L)";
  }
  PON_Total {
    Float32 actual_range 0.3969211, 20.2548;
    String long_name "Pon_total";
    String units "micromoles per liter (umol/L)";
  }
  POC_gt_5um {
    Float32 actual_range -0.4438948, 185.2285;
    String long_name "Poc_gt_5um";
    String units "micromoles per liter (umol/L)";
  }
  POC_GFF {
    Float32 actual_range -0.0020471, 28.16849;
    String long_name "Poc_gff";
    String units "micromoles per liter (umol/L)";
  }
  POC_Total {
    Float32 actual_range 0.0690021, 196.2137;
    String long_name "Poc_total";
    String units "micromoles per liter (umol/L)";
  }
  N_Uptake_gt_5um {
    Float32 actual_range 0.0176493, 9.650486;
    String long_name "N_uptake_gt_5um";
    String units "micromoles per liter per day (umol/L/day)";
  }
  N_Uptake_GFF {
    Float32 actual_range 0.0162029, 1.384334;
    String long_name "N_uptake_gff";
    String units "micromoles per liter per day (umol/L/day)";
  }
  N_Uptake_Total {
    Float32 actual_range 0.0392619, 10.06306;
    String long_name "N_uptake_total";
    String units "micromoles per liter per day (umol/L/day)";
  }
  Biomass_normalized_N_Uptake_gt_5um {
    Float32 actual_range 0.0505332, 0.900757;
    String long_name "Biomass_normalized_n_uptake_gt_5um";
    String units "per day";
  }
  Biomass_normalized_N_Uptake_GFF {
    Float32 actual_range 0.0426665, 0.4607246;
    String long_name "Biomass_normalized_n_uptake_gff";
    String units "per day";
  }
  Biomass_normalized_N_Uptake_Total {
    Float32 actual_range 0.1179996, 1.292037;
    String long_name "Biomass_normalized_n_uptake_total";
    String units "per day";
  }
  C_Uptake_5um {
    Float32 actual_range -0.0439365, 226.1147;
    String long_name "C_uptake_5um";
    String units "micromoles per liter per day (umol/L/day)";
  }
  C_Uptake_GFF {
    Float32 actual_range -1.76e-4, 26.58769;
    String long_name "C_uptake_gff";
    String units "micromoles per liter per day (umol/L/day)";
  }
  C_Uptake_Total {
    Float32 actual_range -0.0045826, 231.3316;
    String long_name "C_uptake_total";
    String units "micromoles per liter per day (umol/L/day)";
  }
  Biomass_normalized_C_Uptake_gt_5um {
    Float32 actual_range 0.0166, 1.5902;
    String long_name "Biomass_normalized_c_uptake_gt_5um";
    String units "per day";
  }
  Biomass_normalized_C_Uptake_GFF {
    Float32 actual_range 0.0128, 0.9587;
    String long_name "Biomass_normalized_c_uptake_gff";
    String units "per day";
  }
  Biomass_normalized_C_Uptake_Total {
    Float32 actual_range 0.0293456, 2.308585;
    String long_name "Biomass_normalized_c_uptake_total";
    String units "per day";
  }
  Particulate_DA {
    Float32 actual_range 0.027, 12.402;
    String long_name "Particulate_da";
    String units "micrograms per liter (ug/L)";
  }
  Dissolved_DA {
    Float32 actual_range 0.0, 12.91;
    String long_name "Dissolved_da";
    String units "micrograms per liter (ug/L)";
  }
  Dissolved_Y {
    Float32 actual_range 101.0231, 194.4722;
    String long_name "Dissolved_y";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Cd {
    Float32 actual_range 100.3228, 796.1678;
    String long_name "Dissolved_cd";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_La {
    Float32 actual_range 15.88603, 51.45339;
    String long_name "Dissolved_la";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Pb {
    Float32 actual_range 16.29469, 82.12563;
    String long_name "Dissolved_pb";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Ce {
    Float32 actual_range 5.275867, 38.30345;
    String long_name "Dissolved_ce";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Sc {
    Float32 actual_range 0.791731, 13.88508;
    String long_name "Dissolved_sc";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Mn {
    Float32 actual_range 0.918466, 10.77443;
    String long_name "Dissolved_mn";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Dissolved_Co {
    Float32 actual_range 62.46153, 563.6546;
    String long_name "Dissolved_co";
    String units "picomoles per kilogram (pmol/kg)";
  }
  Dissolved_Ni {
    Float32 actual_range 3.041688, 10.87644;
    String long_name "Dissolved_ni";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Dissolved_Cu {
    Float32 actual_range 0.7565952, 8.093715;
    String long_name "Dissolved_cu";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Dissolved_Zn {
    Float32 actual_range 0.1360039, 5.341446;
    String long_name "Dissolved_zn";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Dissolved_Fe {
    Float32 actual_range 0.055, 6.038;
    String long_name "Dissolved_fe";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Particulate_Fe_pFe_leached_gt_5um {
    Float32 actual_range 0.181, 16.306;
    String long_name "Particulate_fe_pfe_leached_gt_5um";
    String units "nanomoles per kilogram (nmol/kg)";
  }
  Fe_N_ratio {
    Float32 actual_range 0.0218, 4.6151;
    String long_name "Fe_n_ratio";
    String units "unitless";
  }
  Fe_uptake_lt_5um {
    Float32 actual_range 0.0, 0.897;
    String long_name "Fe_uptake_lt_5um";
    String units "picomoles Fe per liter per hour (pmol Fe/L/h)";
  }
  Fe_uptake_gt_5um {
    Float32 actual_range 0.021, 0.687;
    String long_name "Fe_uptake_gt_5um";
    String units "picomoles Fe per liter per hour (pmol Fe/L/h)";
  }
  Fe_uptake_lt_5um_pmol_Fe_ug_Chla_h {
    Float32 actual_range 0.288, 26.573;
    String long_name "Fe_uptake_lt_5um_pmol_fe_ug_chla_h";
    String units "picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h)";
  }
  Fe_uptake_gt_5um_pmol_Fe_ug_Chla_h {
    Float32 actual_range 0.005, 22.598;
    String long_name "Fe_uptake_gt_5um_pmol_fe_ug_chla_h";
    String units "picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h)";
  }
  Fe_uptake_lt_5um_pmol_Fe_umol_POC_h {
    Float32 actual_range 0.019, 0.648;
    String long_name "Fe_uptake_lt_5um_pmol_fe_umol_poc_h";
    String units "picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h)";
  }
  Fe_uptake_gt_5um_pmol_Fe_umol_POC_h {
    Float32 actual_range 0.001, 0.697;
    String long_name "Fe_uptake_gt_5um_pmol_fe_umol_poc_h";
    String units "picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h)";
  }
 }
  NC_GLOBAL {
    String cdm_data_type "Other";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_url "https://www.bco-dmo.org/";
    String doi "10.26008/1912/bco-dmo.949788.1";
    Float64 Easternmost_Easting -121.5429;
    Float64 geospatial_lat_max 41.01471;
    Float64 geospatial_lat_min 35.92353;
    String geospatial_lat_units "degrees_north";
    Float64 geospatial_lon_max -121.5429;
    Float64 geospatial_lon_min -124.4163;
    String geospatial_lon_units "degrees_east";
    String history 
"2025-08-15T21:20:51Z (local files)
2025-08-15T21:20:51Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949788_v1.html";
    String infoUrl "https://osprey.bco-dmo.org/dataset/949788";
    String institution "BCO-DMO";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    Float64 Northernmost_Northing 41.01471;
    String sourceUrl "(local files)";
    Float64 Southernmost_Northing 35.92353;
    String summary 
"The primary objective of this study was to assess the physiological and molecular response of subsurface phytoplankton communities to a simulation of the Upwelling Conveyor Belt Cycle (UCBC). To capture this phenomenon, we performed on-deck incubation experiments that spanned different time points representing the different stages of growth (initial deep-water community at T0, and stimulated growth phases at T1 and T2). We further subjected the incubations to various Fe-related treatments to test how Fe might play a role in determining their physiological and molecular response to upwelling. 

The first incubation was conducted from 27 May to 01 June 2019 in a wide continental shelf, and the second incubation was conducted from 02 June to 06 June 2019 in a narrow continental shelf. For each time point, the incubation was harvested and analyzed for chlorophyll, nutrients, particulate organic carbon and nitrogen, and dissolved and particulate Fe and Sc concentrations. 6-hour incubations using trace concentrations of stable isotopes of 13C and 15N were also conducted to assess uptake kinetics of dissolved inorganic carbon (DIC) and nitrate (NO3) in bulk-size fractionated phytoplankton communities. PUPCYCLE I (Phytoplankton response to the UPwelling CYCLE) took place in summer 2019 onboard the R/V Oceanus (OC1905b), led by Chief Scientist Adrian Marchetti.";
    String title "[PUPCYCLE 2019 incubation experiments] - Incubation experiments to assess the physiological and molecular response of subsurface phytoplankton communities to a simulation of the Upwelling Conveyor Belt Cycle (UCBC) conducted in summer 2019 onboard the R/V Oceanus (OC1905b) (CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans)";
    Float64 Westernmost_Easting -124.4163;
  }
}

 

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