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Dataset Title:  [flow cytometry] - Flow cytometric analysis of interaction between Alteromonas
macleodii exudates and Prochlorococcus MIT9312 cells (Collaborative Research:
Extracellular vesicles as vehicles for microbial interactions in marine Black
Queen communities)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_994735_v1)
Information:  Summary ? | License ? | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 File (unitless) ?          "2021-08-05_26_Cont..."    "2021-08-05_26_Vesi..."
 Treatment (unitless) ?          "Control"    "Vesicle"
 Analyzed_Events (unitless) ?          10000    10000
 Fixed (unitless) ?          "No"    "No"
 Stained (unitless) ?          "No"    "Yes"
 Dilution (unitless) ?          0.1    0.1
 Prefiltration (unitless) ?          "No"    "No"
 FSC_Mean (arbitrary fluorescence units (AFU)) ?          277.0828    900.4669
 Green_Mean (arbitrary fluorescence units (AFU)) ?          1.27666    70.06373
 Red_Mean (arbitrary fluorescence units (AFU)) ?          146.3078    269.194
 FSC_Median (arbitrary fluorescence units (AFU)) ?          69.3139    554.8054
 Green_Median (arbitrary fluorescence units (AFU)) ?          1.183702    24.63778
 Red_Median (arbitrary fluorescence units (AFU)) ?          117.5743    233.434
 FSC_CV (arbitrary fluorescence units (AFU)) ?          2.395399    8.160698
 Green_CV (arbitrary fluorescence units (AFU)) ?          0.2961332    15.04325
 Red_CV (arbitrary fluorescence units (AFU)) ?          0.5971143    2.861096
 
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File type: (more information)

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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  File {
    String long_name "File";
    String units "unitless";
  }
  Treatment {
    String long_name "Treatment";
    String units "unitless";
  }
  Analyzed_Events {
    Int32 actual_range 10000, 10000;
    String long_name "Analyzed_events";
    String units "unitless";
  }
  Fixed {
    String long_name "Fixed";
    String units "unitless";
  }
  Stained {
    String long_name "Stained";
    String units "unitless";
  }
  Dilution {
    Float32 actual_range 0.1, 0.1;
    String long_name "Dilution";
    String units "unitless";
  }
  Prefiltration {
    String long_name "Prefiltration";
    String units "unitless";
  }
  FSC_Mean {
    Float32 actual_range 277.0828, 900.4669;
    String long_name "Fsc_mean";
    String units "arbitrary fluorescence units (AFU)";
  }
  Green_Mean {
    Float32 actual_range 1.27666, 70.06373;
    String long_name "Green_mean";
    String units "arbitrary fluorescence units (AFU)";
  }
  Red_Mean {
    Float32 actual_range 146.3078, 269.194;
    String long_name "Red_mean";
    String units "arbitrary fluorescence units (AFU)";
  }
  FSC_Median {
    Float32 actual_range 69.3139, 554.8054;
    String long_name "Fsc_median";
    String units "arbitrary fluorescence units (AFU)";
  }
  Green_Median {
    Float32 actual_range 1.183702, 24.63778;
    String long_name "Green_median";
    String units "arbitrary fluorescence units (AFU)";
  }
  Red_Median {
    Float32 actual_range 117.5743, 233.434;
    String long_name "Red_median";
    String units "arbitrary fluorescence units (AFU)";
  }
  FSC_CV {
    Float32 actual_range 2.395399, 8.160698;
    String long_name "Fsc_cv";
    String units "arbitrary fluorescence units (AFU)";
  }
  Green_CV {
    Float32 actual_range 0.2961332, 15.04325;
    String long_name "Green_cv";
    String units "arbitrary fluorescence units (AFU)";
  }
  Red_CV {
    Float32 actual_range 0.5971143, 2.861096;
    String long_name "Red_cv";
    String units "arbitrary fluorescence units (AFU)";
  }
 }
  NC_GLOBAL {
    String cdm_data_type "Other";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_url "https://www.bco-dmo.org/";
    String doi "10.26008/1912/bco-dmo.994735.1";
    String history 
"2026-07-23T07:20:19Z (local files)
2026-07-23T07:20:19Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_994735_v1.html";
    String infoUrl "https://osprey.bco-dmo.org/dataset/994735";
    String institution "BCO-DMO";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    String sourceUrl "(local files)";
    String summary "The cyanobacterium Prochlorococcus has a conspicuously reduced genome causing it to require help from co-existing organisms for survival under a variety of stressful conditions. In this work we used conditioned media experiments to demonstrate that exudates of the heterotrophic bacterium Alteromonas macleodii EZ55 facilitated the survival of Prochlorococcus MIT9312 batch co-cultures as they entered stationary phase. Microscopy revealed the presence of extracellular membrane vesicles and protein complexes in the exudates. When the exudates were stained with an amine-reactive dye, we were able to observe them interacting directly with Prochlorococcus cells by flow cytometry, where stained exudates (but not dye alone) added both green fluorescence and size (forward scatter) to Prochlorococcus cells.";
    String title "[flow cytometry] - Flow cytometric analysis of interaction between Alteromonas macleodii exudates and Prochlorococcus MIT9312 cells (Collaborative Research: Extracellular vesicles as vehicles for microbial interactions in marine Black Queen communities)";
  }
}

 

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tabledap lets you request a data subset, a graph, or a map from a tabular dataset (for example, buoy data), via a specially formed URL. tabledap uses the OPeNDAP (external link) Data Access Protocol (DAP) (external link) and its selection constraints (external link).

The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.

Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names, followed by a collection of constraints (e.g., variable<value), each preceded by '&' (which is interpreted as "AND").

For details, see the tabledap Documentation.


 
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