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     data   graph     files  public [flow cytometry] - Flow cytometric analysis of interaction between Alteromonas macleodii
exudates and Prochlorococcus MIT9312 cells (Collaborative Research: Extracellular vesicles as
vehicles for microbial interactions in marine Black Queen communities)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.994735.1
attribute NC_GLOBAL infoUrl String https://osprey.bco-dmo.org/dataset/994735 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL summary String The cyanobacterium Prochlorococcus has a conspicuously reduced genome causing it to require help from co-existing organisms for survival under a variety of stressful conditions. In this work we used conditioned media experiments to demonstrate that exudates of the heterotrophic bacterium Alteromonas macleodii EZ55 facilitated the survival of Prochlorococcus MIT9312 batch co-cultures as they entered stationary phase. Microscopy revealed the presence of extracellular membrane vesicles and protein complexes in the exudates. When the exudates were stained with an amine-reactive dye, we were able to observe them interacting directly with Prochlorococcus cells by flow cytometry, where stained exudates (but not dye alone) added both green fluorescence and size (forward scatter) to Prochlorococcus cells.
attribute NC_GLOBAL title String [flow cytometry] - Flow cytometric analysis of interaction between Alteromonas macleodii exudates and Prochlorococcus MIT9312 cells (Collaborative Research: Extracellular vesicles as vehicles for microbial interactions in marine Black Queen communities)
variable File   String  
attribute File long_name String File
attribute File units String unitless
variable Treatment   String  
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable Analyzed_Events   int  
attribute Analyzed_Events actual_range int 10000, 10000
attribute Analyzed_Events long_name String Analyzed_events
attribute Analyzed_Events units String unitless
variable Fixed   String  
attribute Fixed long_name String Fixed
attribute Fixed units String unitless
variable Stained   String  
attribute Stained long_name String Stained
attribute Stained units String unitless
variable Dilution   float  
attribute Dilution actual_range float 0.1, 0.1
attribute Dilution long_name String Dilution
attribute Dilution units String unitless
variable Prefiltration   String  
attribute Prefiltration long_name String Prefiltration
attribute Prefiltration units String unitless
variable FSC_Mean   float  
attribute FSC_Mean actual_range float 277.0828, 900.4669
attribute FSC_Mean long_name String Fsc_mean
attribute FSC_Mean units String arbitrary fluorescence units (AFU)
variable Green_Mean   float  
attribute Green_Mean actual_range float 1.27666, 70.06373
attribute Green_Mean long_name String Green_mean
attribute Green_Mean units String arbitrary fluorescence units (AFU)
variable Red_Mean   float  
attribute Red_Mean actual_range float 146.3078, 269.194
attribute Red_Mean long_name String Red_mean
attribute Red_Mean units String arbitrary fluorescence units (AFU)
variable FSC_Median   float  
attribute FSC_Median actual_range float 69.3139, 554.8054
attribute FSC_Median long_name String Fsc_median
attribute FSC_Median units String arbitrary fluorescence units (AFU)
variable Green_Median   float  
attribute Green_Median actual_range float 1.183702, 24.63778
attribute Green_Median long_name String Green_median
attribute Green_Median units String arbitrary fluorescence units (AFU)
variable Red_Median   float  
attribute Red_Median actual_range float 117.5743, 233.434
attribute Red_Median long_name String Red_median
attribute Red_Median units String arbitrary fluorescence units (AFU)
variable FSC_CV   float  
attribute FSC_CV actual_range float 2.395399, 8.160698
attribute FSC_CV long_name String Fsc_cv
attribute FSC_CV units String arbitrary fluorescence units (AFU)
variable Green_CV   float  
attribute Green_CV actual_range float 0.2961332, 15.04325
attribute Green_CV long_name String Green_cv
attribute Green_CV units String arbitrary fluorescence units (AFU)
variable Red_CV   float  
attribute Red_CV actual_range float 0.5971143, 2.861096
attribute Red_CV long_name String Red_cv
attribute Red_CV units String arbitrary fluorescence units (AFU)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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