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Dataset Title:  [Mesopelagic decay experiments: bulk 14C, ATP, and prokaryote and protist
abundances] - Bulk percentage of 14C, ATP, prokaryote and protist abundances
from mesopelagic decay experiments with Thalassiosira weissflogii, Emiliania
huxleyi, and Tetraselmis sp. (Collaborative Research: Transforming Carbon in
the Deep Sea)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_995456_v1)
Information:  Summary ? | License ? | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 Experiment (unitless) ?          "1"    "3"
 Algal_substrate (unitless) ?          "Eh"    "Tw"
 Carbon_pool (unitless) ?          "DOC"    "POC"
 Elapsed_days (days) ?          0.0    87.2
 Pcnt_14C_flask1 (percent (%)) ?          7.33    111.8
 Pcnt_14C_flask2 (percent (%)) ?          5.84    115.51
 Pcnt_14C_flask3 (percent (%)) ?          7.63    127.59
 Decay_1 (per day (d-1)) ?          0.0    1.346
 Decay_2 (per day (d-1)) ?          0.0    1.265
 Decay_3 (per day (d-1)) ?          0.0    1.315
 ATP_flask1 (nM (nanomolar)) ?          0.003    1.125
 ATP_flask2 (nM (nanomolar)) ?          -0.007    1.841
 ATP_flask3 (nM (nanomolar)) ?          0.0    2.446
 Prok_flask1_filter1 (cells per milliliter (cells ml-1)) ?          16100.0    1830000.0
 Prok_flask2_filter1 (cells per milliliter (cells ml-1)) ?          49300.0    1650000.0
 Prok_flask3_filter1 (cells per milliliter (cells ml-1)) ?          53600.0    1260000.0
 Prok_flask1_filter2 (cells per milliliter (cells ml-1)) ?          139000.0    753000.0
 Prok_flask2_filter2 (cells per milliliter (cells ml-1)) ?          229000.0    1330000.0
 Prok_flask3_filter2 (cells per milliliter (cells ml-1)) ?          160000.0    1090000.0
 Prot_flask1 (cells per milliliter (cells ml-1)) ?          0.0    2820.94
 Prot_flask2 (cells per milliliter (cells ml-1)) ?          0.0    6488.15
 Prot_flask3 (cells per milliliter (cells ml-1)) ?          0.0    803.97
 
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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  Experiment {
    String long_name "Experiment";
    String units "unitless";
  }
  Algal_substrate {
    String long_name "Algal_substrate";
    String units "unitless";
  }
  Carbon_pool {
    String long_name "Carbon_pool";
    String units "unitless";
  }
  Elapsed_days {
    Float32 actual_range 0.0, 87.2;
    String long_name "Elapsed_days";
    String units "days";
  }
  Pcnt_14C_flask1 {
    Float32 actual_range 7.33, 111.8;
    String long_name "Pcnt_14c_flask1";
    String units "percent (%)";
  }
  Pcnt_14C_flask2 {
    Float32 actual_range 5.84, 115.51;
    String long_name "Pcnt_14c_flask2";
    String units "percent (%)";
  }
  Pcnt_14C_flask3 {
    Float32 actual_range 7.63, 127.59;
    String long_name "Pcnt_14c_flask3";
    String units "percent (%)";
  }
  Decay_1 {
    Float32 actual_range 0.0, 1.346;
    String long_name "Decay_1";
    String units "per day (d-1)";
  }
  Decay_2 {
    Float32 actual_range 0.0, 1.265;
    String long_name "Decay_2";
    String units "per day (d-1)";
  }
  Decay_3 {
    Float32 actual_range 0.0, 1.315;
    String long_name "Decay_3";
    String units "per day (d-1)";
  }
  ATP_flask1 {
    Float32 actual_range 0.003, 1.125;
    String long_name "Atp_flask1";
    String units "nM (nanomolar)";
  }
  ATP_flask2 {
    Float32 actual_range -0.007, 1.841;
    String long_name "Atp_flask2";
    String units "nM (nanomolar)";
  }
  ATP_flask3 {
    Float32 actual_range 0.0, 2.446;
    String long_name "Atp_flask3";
    String units "nM (nanomolar)";
  }
  Prok_flask1_filter1 {
    Float32 actual_range 16100.0, 1830000.0;
    String long_name "Prok_flask1_filter1";
    String units "cells per milliliter (cells ml-1)";
  }
  Prok_flask2_filter1 {
    Float32 actual_range 49300.0, 1650000.0;
    String long_name "Prok_flask2_filter1";
    String units "cells per milliliter (cells ml-1)";
  }
  Prok_flask3_filter1 {
    Float32 actual_range 53600.0, 1260000.0;
    String long_name "Prok_flask3_filter1";
    String units "cells per milliliter (cells ml-1)";
  }
  Prok_flask1_filter2 {
    Float32 actual_range 139000.0, 753000.0;
    String long_name "Prok_flask1_filter2";
    String units "cells per milliliter (cells ml-1)";
  }
  Prok_flask2_filter2 {
    Float32 actual_range 229000.0, 1330000.0;
    String long_name "Prok_flask2_filter2";
    String units "cells per milliliter (cells ml-1)";
  }
  Prok_flask3_filter2 {
    Float32 actual_range 160000.0, 1090000.0;
    String long_name "Prok_flask3_filter2";
    String units "cells per milliliter (cells ml-1)";
  }
  Prot_flask1 {
    Float32 actual_range 0.0, 2820.94;
    String long_name "Prot_flask1";
    String units "cells per milliliter (cells ml-1)";
  }
  Prot_flask2 {
    Float32 actual_range 0.0, 6488.15;
    String long_name "Prot_flask2";
    String units "cells per milliliter (cells ml-1)";
  }
  Prot_flask3 {
    Float32 actual_range 0.0, 803.97;
    String long_name "Prot_flask3";
    String units "cells per milliliter (cells ml-1)";
  }
 }
  NC_GLOBAL {
    String cdm_data_type "Other";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_url "https://www.bco-dmo.org/";
    String doi "10.26008/1912/bco-dmo.995456.1";
    String history 
"2026-09-18T06:56:09Z (local files)
2026-09-18T06:56:09Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_995456_v1.html";
    String infoUrl "https://osprey.bco-dmo.org/dataset/995456";
    String institution "BCO-DMO";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    String sourceUrl "(local files)";
    String summary 
"The mesopelagic zone is a site of strong microbially driven particle attenuation with depth and thus plays a crucial role in controlling the transfer efficiency of the ocean's biological pump. However, little quantitative information exists on the dependency of decay processes on the source material. Here, we followed the decay of 14C-labeled dead particulate carbon (POC) and dissolved organic carbon (DOC) from three different phytoplankton species, and two incubations of live diatoms, in mesopelagic water over three months. Commonly used first-order kinetics failed to adequately describe the decay of organic material as rate constants varied from day to day. Over extended periods, decay rates for organic material exhibited two distinct phases, with rates in the second phase being inversely related to rates in the first phase. Microbial biomass (measured via ATP and cell counts) increased substantially during phase 1 and ebbed during phase 2. Decay rates were significantly different among the three algal sources; however, differences were even more pronounced among carbon pools and followed a distinct pattern (combined average per-day decay rates at 12 degrees Celsius): fresh DOC (0.6) > fresh POC (0.1) > live cells (0.06) > aged DOC/POC (0.01). Separation of POC into four broad biochemical fractions showed that components in the operationally defined lipid fraction contained the most degradable compounds for fresh material. Our research highlights the need to include the dynamics of the most easily digestible fractions of freshly released organic material, and live plankton resilient to digestion, in calculations of vertical carbon flux budgets. 
 
This dataset includes bulk percent remaining 14C, ATP concentrations, and prokaryote and protist abundances, and instantaneous decay rate calculations from mesopelagic decay experiments using three different 14C-labeled algae (Thalassiosira weissflogii, Tetraselmis sp., and Emiliania huxleyi). See the \"Related Datasets\" section for complementary datasets from the same experiments.";
    String title "[Mesopelagic decay experiments: bulk 14C, ATP, and prokaryote and protist abundances] - Bulk percentage of 14C, ATP, prokaryote and protist abundances from mesopelagic decay experiments with Thalassiosira weissflogii, Emiliania huxleyi, and Tetraselmis sp. (Collaborative Research: Transforming Carbon in the Deep Sea)";
  }
}

 

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