Data Access Form

Dataset Title:	[Alteromonas MIT1002 EV Transcriptome] - NCBI accession metadata for transcriptomic expression sequences from cultures of Alteromonas macleodii MIT1002 grown with bacterial extracellular vesicles as a carbon source (Collaborative Research: Quantifying the role of microbial extracellular vesicles in marine dissolved organic matter production and consumption)
Institution:	BCO-DMO (Dataset ID: bcodmo_dataset_997637_v1)
Information:	Summary \| License \| Metadata \| Background \| Files

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Variable	Minimum	Maximum
Biosample_ID (unitless)	"SAMN54258846"	"SAMN54258869"
SRA_Experiment_ID (unitless)	"SRX31572412"	"SRX31572435"
GEO_Sample_ID (unitless)	"GSM9407407"	"GSM9407430"
Bacterium (unitless)	"Alteromonas macleo..."	"Alteromonas macleo..."
Carbon_Substrate (unitless)	"Alteromonas extrac..."	"none"
Timepoint (unitless)	"1 (early exponenti..."	"2 (late exponentia..."
Replicate (unitless)	1	3

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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  Biosample_ID {
    String long_name "Biosample_id";
    String units "unitless";
  }
  SRA_Experiment_ID {
    String long_name "Sra_experiment_id";
    String units "unitless";
  }
  GEO_Sample_ID {
    String long_name "Geo_sample_id";
    String units "unitless";
  }
  Bacterium {
    String long_name "Bacterium";
    String units "unitless";
  }
  Carbon_Substrate {
    String long_name "Carbon_substrate";
    String units "unitless";
  }
  Timepoint {
    String long_name "Timepoint";
    String units "unitless";
  }
  Replicate {
    Int32 actual_range 1, 3;
    String long_name "Replicate";
    String units "unitless";
  }
 }
  NC_GLOBAL {
    String cdm_data_type "Other";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_url "https://www.bco-dmo.org/";
    String doi "10.26008/1912/bco-dmo.997637.1";
    String history 
"2026-11-07T12:04:00Z (local files)
2026-11-07T12:04:00Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_997637_v1.html";
    String infoUrl "https://osprey.bco-dmo.org/dataset/997637";
    String institution "BCO-DMO";
    String license 
"The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.";
    String sourceUrl "(local files)";
    String summary 
"To examine how marine bacteria utilize extracellular vesicles as a nutrient source, we compared the transcriptomic response of Alteromonas macleodii MIT1002 cells grown on purified extracellular vesicles (purified from Alteromonas MIT1002 or Prochlorococcus MIT9312) as compared to glucose or a no-carbon control. Our study provides insights into the cellular mechanisms through which these cells may take up extracellular vesicle material and the specific types of molecules within vesicles that can be utilized for carbon and/or energy. 

This dataset contains accession metadata for sequences run from cultures of Alteromonas macleodii MIT1002. Data were collected by Dr. Ashley Stein and Dr. Steven Biller, Wellesley College. The raw RNAseq reads have been deposited in the National Center for Biotechnology Information (NCBI) GEO database under accession GSE314643.";
    String title "[Alteromonas MIT1002 EV Transcriptome] - NCBI accession metadata for transcriptomic expression sequences from cultures of Alteromonas macleodii MIT1002 grown with bacterial extracellular vesicles as a carbon source (Collaborative Research: Quantifying the role of microbial extracellular vesicles in marine dissolved organic matter production and consumption)";
  }
}

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