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     data   graph     files  public Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary
System, and Long Island Sound (Alexandrium isotopes project)
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_712027

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Stable Isotope Methods for d15N-NO3-, d18O-NO3-, d15N-POM, and d15N-NH4+: All
filters, glassware, and glass vials used for isotope analysis were pre-
combusted for 4 hours at 450C and plastic bottles were acid washed in a 10%
HCl bath overnight, to eliminate any crossover N. In preparation for stable
isotope analysis, raw seawater was filtered onto a glass fiber filter (25-mm
diameter, Whatman GF/D, Cat #: 1823010) to separate particulate organic matter
(POM) and dissolved fractions (NO3- and NH4+). Filters were transferred into
20-ml glass vials (Fisher, catalog #05-719-117) and frozen at -20C until
isotope analysis of POM (15NPOM). Filtrate, 200 ml, for isotope analysis of
dissolved NH4+ (15NNH4+) was processed using a modified version of the NH4+
diffusion method of Holmes (1998), whereby polypropylene membrane filters (25
mm, lot #: 151579, Sterlitech) replaced Teflon filters (Hannon and Bohlke
2008). Isotope analysis of NH4+ and POM was performed on a Finnigan-MAT
DeltaPlus Isotope Ratio Monitoring Mass Spectrometer coupled with a Carlo Erba
NC 2500 Elemental Analyzer (Model 1108) (Organic Mass Spectrometry Facility,
WHOI). Depending on the expected amount of N per sample, the instrument was
configured for the typical range of detection, 0.5 to 5 moles N, or modified
at the EA combustion furnace to reach a lower detection limit, 0.15 umoles N
(Houghton et al. 2000, Holtvoeth et al. 2005, 2006, York et al. 2007). The
precision of 15N measurements on this instrument was 0.17\u2030. Filtrate, 20
ml for N and O stable isotope analysis of dissolved nitrate (15NNO3-) was
transferred into duplicate 30-ml HDPE bottles and analyzed by bacteria
denitrification assay using ThermoFinnigan GasBench + PreCon trace gas
concentration system interfaced to a ThermoScientific Delta V Plus isotope-
ratio mass spectrometer (Bremen, Germany) (UC Davis Stable Isotope Facility).

Methods for nutrient quantification: Dissolved nutrient samples were filtered
through a glass fiber filter (25-mm diameter, Whatman GF/F, CAT No. 1825-025).
The filtrate was collected in acid-washed 20-ml scintillation vials for
nutrient analysis and then frozen at -20 deg C until analysis. A SEAL AA3
four-channel segmented flow analyzer (Nutrient Chemical Facility, Woods Hole
Oceanographic Institution, WHOI) was used to quantify NH4+, NO3-/NO2-,
Silicate, and PO43- in the GF/F-filtered medium using standard methods.
attribute NC_GLOBAL awards_0_award_nid String 700442
attribute NC_GLOBAL awards_0_award_number String OCE-1505604
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1505604 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL awards_1_award_nid String 700452
attribute NC_GLOBAL awards_1_award_number String OCE-1232835
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1232835 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Dr Henrietta N Edmonds
attribute NC_GLOBAL awards_1_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Chemical data
J. Smith, D. Anderson, D. Erdner, and J. McClelland, PIs
Version 3 August 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv.pl v1.0
attribute NC_GLOBAL date_created String 2017-08-03T17:58:49Z
attribute NC_GLOBAL date_modified String 2019-03-20T16:01:03Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.712027.1
attribute NC_GLOBAL geospatial_vertical_max double 90.0
attribute NC_GLOBAL geospatial_vertical_min double 0.5
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/712027 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to perform isotope analysis of NH4+�and POM
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 712039
attribute NC_GLOBAL instruments_0_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 469
attribute NC_GLOBAL instruments_0_supplied_name String Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer
attribute NC_GLOBAL instruments_1_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to analyze N and O stable isotopes
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 712041
attribute NC_GLOBAL instruments_1_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_1_instrument_nid String 469
attribute NC_GLOBAL instruments_1_supplied_name String ThermoScientific Delta V Plus isotope-ratio mass spectrometer
attribute NC_GLOBAL instruments_2_acronym String Gas Analyzer
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to analyze N and O stable isotopes
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 712042
attribute NC_GLOBAL instruments_2_description String Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
attribute NC_GLOBAL instruments_2_instrument_name String Gas Analyzer
attribute NC_GLOBAL instruments_2_instrument_nid String 720
attribute NC_GLOBAL instruments_2_supplied_name String ThermoFinnigan GasBench + PreCon trace gas concentration system
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Used to perform isotope analysis of NH4+�and POM�
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 712040
attribute NC_GLOBAL instruments_3_description String Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.
attribute NC_GLOBAL instruments_3_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB01/ (external link)
attribute NC_GLOBAL instruments_3_instrument_name String Elemental Analyzer
attribute NC_GLOBAL instruments_3_instrument_nid String 546339
attribute NC_GLOBAL instruments_3_supplied_name String Carlo Erba NC 2500 Elemental Analyzer (Model 1108)
attribute NC_GLOBAL instruments_4_acronym String CFA
attribute NC_GLOBAL instruments_4_dataset_instrument_description String Used to quantify�NH4+, NO3-/NO2-, Silicate, and PO43-
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 712043
attribute NC_GLOBAL instruments_4_description String A sample is injected into a flowing carrier solution passing rapidly through small-bore tubing.�
attribute NC_GLOBAL instruments_4_instrument_name String Continuous Flow Analyzer
attribute NC_GLOBAL instruments_4_instrument_nid String 661968
attribute NC_GLOBAL instruments_4_supplied_name String SEAL AA3 four-channel segmented flow analyzer
attribute NC_GLOBAL keywords String ammonia, ammonium, bco, bco-dmo, biological, chemical, chemistry, concentration, d15, d15N_NH4, d15N_NO3, d15N_POM, d18O_NO3, data, dataset, date, depth, description, dissolved, dissolved nutrients, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Chemistry > Phosphate, Earth Science > Oceans > Ocean Chemistry > Silicate, erddap, identifier, management, mass, mass_concentration_of_phosphate_in_sea_water, mass_concentration_of_silicate_in_sea_water, model, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrate_in_sea_water, n02, nh4, nitrate, no3, nutrients, ocean, oceanography, oceans, office, phosphate, po4, pom, preliminary, princeton, sample, Sample_ID, science, sea, seawater, silicate, statistics, time, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/712027 (external link)
attribute NC_GLOBAL param_mapping String {'712027': {'Depth': 'master - depth'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/712027/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Virginia Institute of Marine Science
attribute NC_GLOBAL people_0_affiliation_acronym String VIMS
attribute NC_GLOBAL people_0_person_name String Dr Juliette L. Smith
attribute NC_GLOBAL people_0_person_nid String 700445
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI
attribute NC_GLOBAL people_1_person_name String Dr Donald M. Anderson
attribute NC_GLOBAL people_1_person_nid String 51236
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Texas - Marine Science Institute
attribute NC_GLOBAL people_2_affiliation_acronym String UTMSI
attribute NC_GLOBAL people_2_person_name String Dr Deana L. Erdner
attribute NC_GLOBAL people_2_person_nid String 700454
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String University of Texas - Marine Science Institute
attribute NC_GLOBAL people_3_affiliation_acronym String UTMSI
attribute NC_GLOBAL people_3_person_name String Dr James W. McClelland
attribute NC_GLOBAL people_3_person_nid String 700456
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String Virginia Institute of Marine Science
attribute NC_GLOBAL people_4_affiliation_acronym String VIMS
attribute NC_GLOBAL people_4_person_name String Dr Juliette L. Smith
attribute NC_GLOBAL people_4_person_nid String 700445
attribute NC_GLOBAL people_4_role String Contact
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL people_5_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_5_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_5_person_name String Hannah Ake
attribute NC_GLOBAL people_5_person_nid String 650173
attribute NC_GLOBAL people_5_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_5_role_type String related
attribute NC_GLOBAL project String Collaborative Research: Identification of nitrogen sources for toxic Alexandrium blooms using a novel species-specific tracer, d15N-saxitoxin
attribute NC_GLOBAL projects_0_acronym String Alexandrium-isotopes
attribute NC_GLOBAL projects_0_description String NSF award abstract:
The US and other countries throughout the world are affected by harmful algal blooms (HABs) that negatively impact human health, marine ecosystems, fisheries resources, and local economies. Anthropogenic nutrient loadings have been linked to expanding HAB incidence, but the relationship is site- and organism-specific, and is still poorly understood. The main challenge in this regard is to determine the relative importance of natural versus anthropogenic nutrient sources in the development of an individual HAB species. Given the diverse nature of the planktonic assemblage in which HABs occur, and the lack of appropriate measurement techniques, this is exceedingly difficult to accomplish.
In this project, research teams at the Woods Hole Oceanographic Institution and University of Texas at Austin will take a novel approach to this challenge: They use use the nitrogen isotopic signature (del15N) of a species-specific HAB toxin to identify the nitrogen source and chemical form that promotes cell growth and toxin production. The bloom-forming dinoflagellate Alexandrium fundyense and its class of bioactive compounds, saxitoxins (STXs), are an ideal model system as STXs are nitrogen-rich and are typically only produced by a single species in mixed plankton assemblages. The guiding overall hypothesis is that the isotopic signature of a HAB-specific toxin can be used to discriminate between anthropogenic and natural sources of N and provide more details than bulk material del15N on the source, chemical form, and processing of N that lead to blooms of a particular toxic species. This hypothesis is based on the principle that human and animal waste in groundwater and sewage become 15N-enriched and inorganic fertilizers 15N-depleted, relative to natural sources of N in catchment waters. While the use of the isotopic ratio del15N of bulk biomass to identify nitrogen sources to coastal waters is a widely accepted practice, this use of a toxin as a species-specific tracer or marker is new and will provide details on the explicit source, chemical form, and processing of nitrogen that results in blooms of a particular HAB species.
Broader Impacts: This project addresses fundamental issues underlying the most widespread of all HAB poisoning syndromes, paralytic shellfish poisoning (PSP), a major form of shellfish poisoning that affects countries throughout the world. Project results can also assist in policy decisions about pollution control and other bloom mitigation strategies, and can be applied to a range of HAB species - those that produce saxitoxins, as well as those that produce other toxins that are nitrogen rich. Project results will be broadly disseminated through scientific papers, presentations at workshops, domestic and international conferences, and departmental seminars, and discussions with the media.
attribute NC_GLOBAL projects_0_end_date String 2016-10
attribute NC_GLOBAL projects_0_geolocation String Gulf of Maine, Nauset Marsh Estuary System (Cape Cod Seashore), Northport Huntington Bay Complex (Long Island Sound)
attribute NC_GLOBAL projects_0_name String Collaborative Research: Identification of nitrogen sources for toxic Alexandrium blooms using a novel species-specific tracer, d15N-saxitoxin
attribute NC_GLOBAL projects_0_project_nid String 700443
attribute NC_GLOBAL projects_0_project_website String http://www.vims.edu/research/departments/eaah/programs/aquatic_toxinology/research/isotope_project.php (external link)
attribute NC_GLOBAL projects_0_start_date String 2014-08
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary System, and Long Island Sound (Alexandrium isotopes project)
attribute NC_GLOBAL title String Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary System, and Long Island Sound (Alexandrium isotopes project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.0-alpha
variable Date   String  
attribute Date description String Date sample was taken; YYYY/MM/DD
attribute Date ioos_category String Time
attribute Date long_name String Date
attribute Date source_name String Date
attribute Date units String unitless
variable Sample_ID   String  
attribute Sample_ID description String Sample ID; short name/abbreviation
attribute Sample_ID ioos_category String Identifier
attribute Sample_ID long_name String Sample ID
attribute Sample_ID units String unitless
variable Description   String  
attribute Description description String Full description of site and station where sample was taken
attribute Description ioos_category String Unknown
attribute Description long_name String Description
attribute Description units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 0.5, 90.0
attribute depth axis String Z
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Depth that sample was taken
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable d15N_NO3   String  
attribute d15N_NO3 colorBarMaximum double 50.0
attribute d15N_NO3 colorBarMinimum double 0.0
attribute d15N_NO3 description String Stable isotope value; d15N - NO3
attribute d15N_NO3 ioos_category String Dissolved Nutrients
attribute d15N_NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute d15N_NO3 units String per mil (0/00)
variable d18O_NO3   String  
attribute d18O_NO3 colorBarMaximum double 50.0
attribute d18O_NO3 colorBarMinimum double 0.0
attribute d18O_NO3 description String Stable isotope value; d18O - NO3
attribute d18O_NO3 ioos_category String Dissolved Nutrients
attribute d18O_NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute d18O_NO3 units String per mil (0/00)
variable d15N_NH4   float  
attribute d15N_NH4 _FillValue float NaN
attribute d15N_NH4 actual_range float 1.4, 17.9
attribute d15N_NH4 colorBarMaximum double 5.0
attribute d15N_NH4 colorBarMinimum double 0.0
attribute d15N_NH4 description String Stable isotope value; d15N - NH4
attribute d15N_NH4 ioos_category String Dissolved Nutrients
attribute d15N_NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute d15N_NH4 units String per mil (0/00)
variable d15N_POM   String  
attribute d15N_POM description String Stable isotope value; d15N - POM
attribute d15N_POM ioos_category String Statistics
attribute d15N_POM long_name String D15 N POM
attribute d15N_POM units String per mil (0/00)
variable NO3   String  
attribute NO3 colorBarMaximum double 50.0
attribute NO3 colorBarMinimum double 0.0
attribute NO3 description String Dissolved nutrient concentration; NO3-
attribute NO3 ioos_category String Dissolved Nutrients
attribute NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute NO3 units String micromoles per liter
variable NH4   float  
attribute NH4 _FillValue float NaN
attribute NH4 actual_range float 0.1, 18.9
attribute NH4 colorBarMaximum double 5.0
attribute NH4 colorBarMinimum double 0.0
attribute NH4 description String Dissolved nutrient concentration; NH4
attribute NH4 ioos_category String Dissolved Nutrients
attribute NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute NH4 units String micromoles per liter
variable PO4   String  
attribute PO4 description String Dissolved nutrient concentration; PO4
attribute PO4 ioos_category String Dissolved Nutrients
attribute PO4 long_name String Mass Concentration Of Phosphate In Sea Water
attribute PO4 units String micromoles per liter
variable Silicate   float  
attribute Silicate _FillValue float NaN
attribute Silicate actual_range float 0.1, 41.8
attribute Silicate description String Dissolved nutrient concentration; Silicate
attribute Silicate ioos_category String Dissolved Nutrients
attribute Silicate long_name String Mass Concentration Of Silicate In Sea Water
attribute Silicate units String micromoles per liter

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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