ERDDAP > info > bcodmo_dataset_716955

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Prochlorococcus in situ cell cycle phases] - Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010. (Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach)		F   I   M	background			BCO-DMO	bcodmo_dataset_716955

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute	NC_GLOBAL	acquisition_description	String	Samples were taken using a rosette of Niskin bottles, fixed with freshly titrated paraformaldehyde (pH 7.4\u20138.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by dual beam flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order, defrosted in a 30\u00b0C water bath (just long enough to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5 ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (Flow Check\u00ae 1.0 um (YG) and 0.494 um (BB); Polysicences Inc., Washington, PA, USA), were added to each sample, and used to normalize cellular light scatter, red (chlorophyll-derived) fluorescence, and Hoechst fluorescence. Samples were run at an infusion rate of 10 uL min-1 for 10 to 50 min, depending on cell abundance within the sample. A minimum of 10,000 Prochlorococcus cells were analyzed, except for samples in which low Prochlorococcus concentrations made this impractical.\u00a0\u00a0 DNA frequency distributions for Prochlorococcus cells were obtained from Hoechst-derived blue fluorescence. These frequency distributions were deconvoluted into their component cell cycle stages (G1, S, G2) using Modfit software (Verity Software House, Topsham, ME, USA), and assuming a simple model comprised of two Gaussian populations (G1 and G2) and a broadened rectangle (S).\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	709338
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0751672
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0751672
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Prochloroccus in situ Cell Cycle Phases Fractions B. Binder, PI Version 12 October 2017
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2017-10-13T20:34:10Z
attribute	NC_GLOBAL	date_modified	String	2019-03-19T16:11:12Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.716955.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-71.998
attribute	NC_GLOBAL	geospatial_lat_max	double	30.874
attribute	NC_GLOBAL	geospatial_lat_min	double	30.158
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-71.998
attribute	NC_GLOBAL	geospatial_lon_min	double	-72.756
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	125.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	40.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/716955
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to take samples in rosette
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	716962
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_1_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to analyze preserved samples
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	716963
attribute	NC_GLOBAL	instruments_1_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Coulter-EPICS 753 flow cytometer
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, cruise, data, dataset, date, date_UTC, depth, dmo, erddap, G1_pc, G1G2_CV, G2_pc, G2G1Ratio, iso, latitude, longitude, management, oceanography, office, preliminary, ratio, S_pc, time, time_UTC
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/716955/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/716955
attribute	NC_GLOBAL	Northernmost_Northing	double	30.874
attribute	NC_GLOBAL	param_mapping	String	{'716955': {'Lat': 'master - latitude', 'Depth': 'flag - depth', 'Lon': 'master - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/716955/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Georgia
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UGA
attribute	NC_GLOBAL	people_0_person_name	String	Dr Brian Binder
attribute	NC_GLOBAL	people_0_person_nid	String	50893
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Picophytoplankton_Regulation
attribute	NC_GLOBAL	projects_0_acronym	String	Picophytoplankton_Regulation
attribute	NC_GLOBAL	projects_0_description	String	The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general. Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum. The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-02
attribute	NC_GLOBAL	projects_0_geolocation	String	Western Sargasso Sea (vicinity of 30 N 72 W)
attribute	NC_GLOBAL	projects_0_name	String	Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
attribute	NC_GLOBAL	projects_0_project_nid	String	709339
attribute	NC_GLOBAL	projects_0_start_date	String	2008-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	30.158
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
attribute	NC_GLOBAL	time_coverage_end	String	2010-05-31T13:29:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2009-05-26T17:58:00Z
attribute	NC_GLOBAL	title	String	[Prochlorococcus in situ cell cycle phases] - Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010. (Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-72.756
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Cruise		String
attribute	Cruise	bcodmo_name	String	cruise_id
attribute	Cruise	description	String	R/V Cape Hatteras Cruise Designation
attribute	Cruise	long_name	String	Cruise
attribute	Cruise	units	String	unitless
variable	Wx		String
attribute	Wx	bcodmo_name	String	exp_id
attribute	Wx	description	String	Experiment Designation
attribute	Wx	long_name	String	WX
attribute	Wx	units	String	unitless
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	40.0, 125.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	Sample Depth
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	date_UTC		String
attribute	date_UTC	bcodmo_name	String	date
attribute	date_UTC	description	String	Sampling date; yyyy/mm/dd
attribute	date_UTC	long_name	String	Date UTC
attribute	date_UTC	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date_UTC	time_precision	String	1970-01-01
attribute	date_UTC	units	String	unitless
variable	time_UTC		String
attribute	time_UTC	bcodmo_name	String	time
attribute	time_UTC	description	String	Sampling time; hh:mm
attribute	time_UTC	long_name	String	Time UTC
attribute	time_UTC	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/
attribute	time_UTC	units	String	unitless
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-72.756, -71.998
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude; N is positive
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	30.158, 30.874
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude; E is positive
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	G1_pc		float
attribute	G1_pc	_FillValue	float	NaN
attribute	G1_pc	actual_range	float	29.1, 98.8
attribute	G1_pc	bcodmo_name	String	cell_concentration
attribute	G1_pc	description	String	Fraction of Prochlorococcus in G1 phase
attribute	G1_pc	long_name	String	G1 PC
attribute	G1_pc	units	String	percent
variable	S_pc		float
attribute	S_pc	_FillValue	float	NaN
attribute	S_pc	actual_range	float	0.0, 54.6
attribute	S_pc	bcodmo_name	String	cell_concentration
attribute	S_pc	description	String	Fraction of Prochlorococcus in S phase
attribute	S_pc	long_name	String	S pc
attribute	S_pc	units	String	percent
variable	G2_pc		float
attribute	G2_pc	_FillValue	float	NaN
attribute	G2_pc	actual_range	float	0.0, 34.6
attribute	G2_pc	bcodmo_name	String	cell_concentration
attribute	G2_pc	description	String	Fraction of Prochlorococcus in G2 phase
attribute	G2_pc	long_name	String	G2 PC
attribute	G2_pc	units	String	percent
variable	G1G2_CV		float
attribute	G1G2_CV	_FillValue	float	NaN
attribute	G1G2_CV	actual_range	float	6.4, 21.6
attribute	G1G2_CV	bcodmo_name	String	unknown
attribute	G1G2_CV	description	String	Coefficient of Variation for Cell Cycle subpopulations
attribute	G1G2_CV	long_name	String	G1 G2 CV
attribute	G1G2_CV	units	String	percent
variable	G2G1Ratio		float
attribute	G2G1Ratio	_FillValue	float	NaN
attribute	G2G1Ratio	actual_range	float	1.85, 2.01
attribute	G2G1Ratio	bcodmo_name	String	fluorescence
attribute	G2G1Ratio	description	String	G2:G1 peak fluorescence ratio
attribute	G2G1Ratio	long_name	String	G2 G1 Ratio
attribute	G2G1Ratio	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	G2G1Ratio	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.24336068E9, 1.27531254E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	DateTime UTC; ISO formatted
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.