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Title Sum-
Institution Dataset ID
     data   graph     files  public Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and
CH0510 in the Western Sargasso Sea in 2009 and 2010.
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Samples were taken using a rosette of Niskin bottles, fixed with freshly
titrated paraformaldehyde (pH 7.4\u20138.1, 0.1% final concentration), held in
the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C
freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until
analysis. Preserved samples were analyzed by dual beam flow cytometry on a
modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were
chosen in random order, defrosted in a 30\u00b0C water bath (just long enough
to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5
ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum
of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (Flow
Check\u00ae 1.0 um (YG) and 0.494 um (BB); Polysicences Inc., Washington, PA,
USA), were added to each sample, and used to normalize cellular light scatter,
red (chlorophyll-derived) fluorescence, and Hoechst fluorescence.

Samples were run at an infusion rate of 10 uL min-1 for 10 to 50 min,
depending on cell abundance within the sample. A minimum of 10,000
Prochlorococcus cells were analyzed, except for samples in which low
Prochlorococcus concentrations made this impractical.\u00a0\u00a0

DNA frequency distributions for Prochlorococcus cells were obtained from
Hoechst-derived blue fluorescence. These frequency distributions were
deconvoluted into their component cell cycle stages (G1, S, G2) using Modfit
software (Verity Software House, Topsham, ME, USA), and assuming a simple
model comprised of two Gaussian populations (G1 and G2) and a broadened
rectangle (S).\u00a0
attribute NC_GLOBAL awards_0_award_nid String 709338
attribute NC_GLOBAL awards_0_award_number String OCE-0751672
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0751672 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Prochloroccus in situ Cell Cycle Phases Fractions
B. Binder, PI
Version 12 October 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-10-13T20:34:10Z
attribute NC_GLOBAL date_modified String 2019-03-19T16:11:12Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.716955.1
attribute NC_GLOBAL Easternmost_Easting double -71.998
attribute NC_GLOBAL geospatial_lat_max double 30.874
attribute NC_GLOBAL geospatial_lat_min double 30.158
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -71.998
attribute NC_GLOBAL geospatial_lon_min double -72.756
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 125.0
attribute NC_GLOBAL geospatial_vertical_min double 40.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/716955 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to take samples in rosette
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 716962
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String Niskin bottle
attribute NC_GLOBAL instruments_1_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to analyze preserved samples
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 716963
attribute NC_GLOBAL instruments_1_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_1_instrument_nid String 660
attribute NC_GLOBAL instruments_1_supplied_name String Coulter-EPICS 753 flow cytometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, cruise, data, dataset, date, date_UTC, depth, dmo, erddap, G1_pc, G1G2_CV, G2_pc, G2G1Ratio, iso, latitude, longitude, management, oceanography, office, preliminary, ratio, S_pc, time, time_UTC
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/716955/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/716955 (external link)
attribute NC_GLOBAL Northernmost_Northing double 30.874
attribute NC_GLOBAL param_mapping String {'716955': {'Lat': 'master - latitude', 'Depth': 'flag - depth', 'Lon': 'master - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/716955/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Dr Brian Binder
attribute NC_GLOBAL people_0_person_nid String 50893
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Picophytoplankton_Regulation
attribute NC_GLOBAL projects_0_acronym String Picophytoplankton_Regulation
attribute NC_GLOBAL projects_0_description String The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general.
Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum.
The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.
attribute NC_GLOBAL projects_0_end_date String 2013-02
attribute NC_GLOBAL projects_0_geolocation String Western Sargasso Sea (vicinity of 30 N 72 W)
attribute NC_GLOBAL projects_0_name String Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
attribute NC_GLOBAL projects_0_project_nid String 709339
attribute NC_GLOBAL projects_0_start_date String 2008-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 30.158
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
attribute NC_GLOBAL time_coverage_end String 2010-05-31T13:29:00Z
attribute NC_GLOBAL time_coverage_start String 2009-05-26T17:58:00Z
attribute NC_GLOBAL title String Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -72.756
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Cruise   String  
attribute Cruise bcodmo_name String cruise_id
attribute Cruise description String R/V Cape Hatteras Cruise Designation
attribute Cruise long_name String Cruise
attribute Cruise units String unitless
variable Wx   String  
attribute Wx bcodmo_name String exp_id
attribute Wx description String Experiment Designation
attribute Wx long_name String WX
attribute Wx units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 40.0, 125.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Sample Depth
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable date_UTC   String  
attribute date_UTC bcodmo_name String date
attribute date_UTC description String Sampling date; yyyy/mm/dd
attribute date_UTC long_name String Date UTC
attribute date_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date_UTC time_precision String 1970-01-01
attribute date_UTC units String unitless
variable time_UTC   String  
attribute time_UTC bcodmo_name String time
attribute time_UTC description String Sampling time; hh:mm
attribute time_UTC long_name String Time UTC
attribute time_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute time_UTC units String unitless
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -72.756, -71.998
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude; N is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 30.158, 30.874
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude; E is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable G1_pc   float  
attribute G1_pc _FillValue float NaN
attribute G1_pc actual_range float 29.1, 98.8
attribute G1_pc bcodmo_name String cell_concentration
attribute G1_pc description String Fraction of Prochlorococcus in G1 phase
attribute G1_pc long_name String G1 PC
attribute G1_pc units String percent
variable S_pc   float  
attribute S_pc _FillValue float NaN
attribute S_pc actual_range float 0.0, 54.6
attribute S_pc bcodmo_name String cell_concentration
attribute S_pc description String Fraction of Prochlorococcus in S phase
attribute S_pc long_name String S pc
attribute S_pc units String percent
variable G2_pc   float  
attribute G2_pc _FillValue float NaN
attribute G2_pc actual_range float 0.0, 34.6
attribute G2_pc bcodmo_name String cell_concentration
attribute G2_pc description String Fraction of Prochlorococcus in G2 phase
attribute G2_pc long_name String G2 PC
attribute G2_pc units String percent
variable G1G2_CV   float  
attribute G1G2_CV _FillValue float NaN
attribute G1G2_CV actual_range float 6.4, 21.6
attribute G1G2_CV bcodmo_name String unknown
attribute G1G2_CV description String Coefficient of Variation for Cell Cycle subpopulations
attribute G1G2_CV long_name String G1 G2 CV
attribute G1G2_CV units String percent
variable G2G1Ratio   float  
attribute G2G1Ratio _FillValue float NaN
attribute G2G1Ratio actual_range float 1.85, 2.01
attribute G2G1Ratio bcodmo_name String fluorescence
attribute G2G1Ratio description String G2:G1 peak fluorescence ratio
attribute G2G1Ratio long_name String G2 G1 Ratio
attribute G2G1Ratio nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute G2G1Ratio units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.24336068E9, 1.27531254E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String DateTime UTC; ISO formatted
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String ISO_DateTime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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