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Title Sum-
Institution Dataset ID
     data   graph     files  public Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA
Resilience project)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Samples were collected at 0 (initial) and 24 hours and parameters of carbonate
chemistry were measured (DIC,CO2, HCO3,CO3-described below). pH drift assay
experiments consisted of 2 independent variables; inhibitor treatment and
algal presence, with 4 and 2 levels, respectively for each independent
variable. The inhibitor treatments included: seawater only (control),
Acetazolamide (A), 4,4\u2019-Diisothiocyano-2,2\u2019-stilbenedisulfonic acid
(DIDS), and Ethoxyzolamide (E). Algae were either present or absent (control)
in a vial. Two replicate water samples were collected from each of 10
experimental culture pots of algae grown for 3 weeks at different combinations
of average and standard deviations of pCO2 level during each of 3 experimental
trials and assayed after 24 hours with or without the presence of Ulva and
combinations of inhibitor treatments. In addition, 3 different levels of pCO2
in seawater generated independently of the seawater in algal culture that
represented the range of pCO2 levels assayed across the entire experiment were
assayed over the same 24 hour period.

Carbonate chemistry parameters were measured by sampling pH and total
alkalinity (TA) of water samples. pH was determined using the m-cresol
indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples
were analyzed by potentiometric titration coupled to a pH electrode calibrated
using certified reference material (CRM) from the Dickson laboratory at
Scripps Oceanographic Institute and the pH electrode calibrated using TRIS
buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from
potentiometric titration data and spectrophotometric pH data.
attribute NC_GLOBAL awards_0_award_nid String 55177
attribute NC_GLOBAL awards_0_award_number String OCE-1316198
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String pH_Drift - Ulva
Carbonate Chemistry over a time course with Ulva in pH drift experiments
PI's: J. Kubler, S. Dudgeon (CSU-Northbridge)
version: 2018-03-22
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-03-28T12:53:51Z
attribute NC_GLOBAL date_modified String 2019-06-03T17:36:03Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.732464.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/732464 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure pH in total scale at 25C.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 732470
attribute NC_GLOBAL instruments_0_description String The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_instrument_nid String 595
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu UV-2450 UV-visible spectrophotometer
attribute NC_GLOBAL instruments_1_acronym String Multi Parameter Bench Meter
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to measure salinity and temperature
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 732474
attribute NC_GLOBAL instruments_1_description String An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and Temperature with one device.
attribute NC_GLOBAL instruments_1_instrument_name String Multi Parameter Bench Meter
attribute NC_GLOBAL instruments_1_instrument_nid String 680
attribute NC_GLOBAL instruments_1_supplied_name String Thermo Fisher Orion Star 329 pH, temperature and dissolved oxygen meter
attribute NC_GLOBAL instruments_2_acronym String Automatic titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to measure total alkalinity.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 732475
attribute NC_GLOBAL instruments_2_description String Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB12/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Automatic titrator
attribute NC_GLOBAL instruments_2_instrument_nid String 682
attribute NC_GLOBAL instruments_2_supplied_name String Mettler Toledo T50 equipped with Rondolino
attribute NC_GLOBAL instruments_3_acronym String MFC
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Settings: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 732473
attribute NC_GLOBAL instruments_3_description String Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute NC_GLOBAL instruments_3_instrument_name String Mass Flow Controller
attribute NC_GLOBAL instruments_3_instrument_nid String 712
attribute NC_GLOBAL instruments_3_supplied_name String Qubit Systems Mass Flow Controllers (MFC)
attribute NC_GLOBAL instruments_4_acronym String Aquarium chiller
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 732472
attribute NC_GLOBAL instruments_4_description String Immersible or in-line liquid cooling device, usually with temperature control.
attribute NC_GLOBAL instruments_4_instrument_name String Aquarium chiller
attribute NC_GLOBAL instruments_4_instrument_nid String 522982
attribute NC_GLOBAL instruments_4_supplied_name String Temperature control chiller: Aqua Logic Cyclone Chiller
attribute NC_GLOBAL keywords String alk, altimetry, average, bco, bco-dmo, biological, carbon, carbon dioxide, carbonate, chemical, co2, CO2_t0, CO2_t24, co3, CO3_t0, CO3_t24, data, dataset, dic, DIC_t0, DIC_t24, dioxide, dmo, erddap, hco3, HCO3_t0, HCO3_t24, label, laboratory, management, oceanography, office, pCO2_avg, pCO2_sd, pH_t0, pH_t24, preliminary, sal, Sal_in, satellite, t24, Temp_in, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/732464/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/732464 (external link)
attribute NC_GLOBAL param_mapping String {'732464': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/732464/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String California State University Northridge
attribute NC_GLOBAL people_0_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_0_person_name String Dr Janet E Kubler
attribute NC_GLOBAL people_0_person_nid String 51681
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String California State University Northridge
attribute NC_GLOBAL people_1_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_1_person_name String Dr Steve Dudgeon
attribute NC_GLOBAL people_1_person_nid String 51682
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_acronym String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_description String Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.
attribute NC_GLOBAL projects_0_end_date String 2016-05
attribute NC_GLOBAL projects_0_geolocation String Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
attribute NC_GLOBAL projects_0_project_nid String 2275
attribute NC_GLOBAL projects_0_start_date String 2013-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Carbonate chemistry measurements over a time course with Ulva in pH drift experiments.
attribute NC_GLOBAL title String Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Label   String  
attribute Label bcodmo_name String sample
attribute Label description String Trial number-pot number.frozen tissue replicate
attribute Label long_name String Label
attribute Label nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Label units String unitless
variable pCO2_avg   short  
attribute pCO2_avg _FillValue short 32767
attribute pCO2_avg actual_range short 248, 1200
attribute pCO2_avg bcodmo_name String pCO2
attribute pCO2_avg description String Average pCO2 partial pressure in seawater tanks
attribute pCO2_avg long_name String P CO2 Avg
attribute pCO2_avg nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_avg units String microatmospheres (µatm)
variable pCO2_sd   float  
attribute pCO2_sd _FillValue float NaN
attribute pCO2_sd actual_range float 0.0, 1827.8
attribute pCO2_sd bcodmo_name String pCO2
attribute pCO2_sd colorBarMaximum double 50.0
attribute pCO2_sd colorBarMinimum double 0.0
attribute pCO2_sd description String Variability of pCO2 partial pressure - standard deviation
attribute pCO2_sd long_name String P CO2 Sd
attribute pCO2_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_sd units String microatmospheres (µatm)
variable Sal_in   float  
attribute Sal_in _FillValue float NaN
attribute Sal_in actual_range float 33.26, 34.11
attribute Sal_in bcodmo_name String sal
attribute Sal_in description String Salinity in situ
attribute Sal_in long_name String Sal In
attribute Sal_in nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute Sal_in units String parts per thousand (ppt)
variable Temp_in   float  
attribute Temp_in _FillValue float NaN
attribute Temp_in actual_range float 15.0, 15.5
attribute Temp_in bcodmo_name String temperature
attribute Temp_in description String Temperature in situ
attribute Temp_in long_name String Temp In
attribute Temp_in nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp_in units String degrees Celsius
variable ALK   double  
attribute ALK _FillValue double NaN
attribute ALK actual_range double 2022.379885, 2700.834795
attribute ALK bcodmo_name String TALK
attribute ALK description String Total Alkalinity measured
attribute ALK long_name String ALK
attribute ALK nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/MDMAP014/ (external link)
attribute ALK units String micromoles/kilogram
variable pH_t0   double  
attribute pH_t0 _FillValue double NaN
attribute pH_t0 actual_range double 7.62199782, 8.72
attribute pH_t0 bcodmo_name String pH
attribute pH_t0 description String initial pH of vial
attribute pH_t0 long_name String P H T0
attribute pH_t0 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH_t0 units String unitless
variable pH_t24   float  
attribute pH_t24 _FillValue float NaN
attribute pH_t24 actual_range float 7.0, 10.32
attribute pH_t24 bcodmo_name String pH
attribute pH_t24 description String pH of vial after 24 hours
attribute pH_t24 long_name String P H T24
attribute pH_t24 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH_t24 units String unitless
variable DIC_t0   double  
attribute DIC_t0 _FillValue double NaN
attribute DIC_t0 actual_range double 1879.275588, 2348.12265
attribute DIC_t0 bcodmo_name String DIC
attribute DIC_t0 description String initial dissolved inorganic carbon in vial
attribute DIC_t0 long_name String DIC T0
attribute DIC_t0 units String micromoles/kilogram (µmol/kg)
variable DIC_t24   double  
attribute DIC_t24 _FillValue double NaN
attribute DIC_t24 actual_range double 627.8257956, 2713.309643
attribute DIC_t24 bcodmo_name String DIC
attribute DIC_t24 description String Dissolved inorganic carbon after 24 hours
attribute DIC_t24 long_name String DIC T24
attribute DIC_t24 units String micromoles/kilogram (µmol/kg)
variable CO2_t0   double  
attribute CO2_t0 _FillValue double NaN
attribute CO2_t0 actual_range double 2.337058831, 38.99218085
attribute CO2_t0 bcodmo_name String TCO2
attribute CO2_t0 description String Initial value of CO2 in vial
attribute CO2_t0 long_name String CO2 T0
attribute CO2_t0 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TCO2KG01/ (external link)
attribute CO2_t0 units String micromoles/kilogram (µmol/kg)
variable CO2_t24   double  
attribute CO2_t24 _FillValue double NaN
attribute CO2_t24 actual_range double 0.002079316, 184.5790984
attribute CO2_t24 bcodmo_name String TCO2
attribute CO2_t24 description String CO2 in vial after 24 hours
attribute CO2_t24 long_name String CO2 T24
attribute CO2_t24 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TCO2KG01/ (external link)
attribute CO2_t24 units String micromoles/kilogram (µmol/kg)
variable HCO3_t0   double  
attribute HCO3_t0 _FillValue double NaN
attribute HCO3_t0 actual_range double 1404.682423, 2126.545747
attribute HCO3_t0 bcodmo_name String bicarbonate
attribute HCO3_t0 description String Initial bicarbonate in vial
attribute HCO3_t0 long_name String HCO3 T0
attribute HCO3_t0 units String micromoles/kilogram (µmol/kg)
variable HCO3_t24   double  
attribute HCO3_t24 _FillValue double NaN
attribute HCO3_t24 actual_range double 46.64740548, 2579.09681
attribute HCO3_t24 bcodmo_name String bicarbonate
attribute HCO3_t24 description String Bicarbonate in vial after 24 hours
attribute HCO3_t24 long_name String HCO3 T24
attribute HCO3_t24 units String micromoles/kilogram (µmol/kg)
variable CO3_t0   double  
attribute CO3_t0 _FillValue double NaN
attribute CO3_t0 actual_range double 57.6173099, 536.8659742
attribute CO3_t0 bcodmo_name String carbonate
attribute CO3_t0 description String Initial carbonate in vial
attribute CO3_t0 long_name String CO3 T0
attribute CO3_t0 units String micromoles/kilogram (µmol/kg)
variable CO3_t24   double  
attribute CO3_t24 _FillValue double NaN
attribute CO3_t24 actual_range double 14.97124859, 956.4429539
attribute CO3_t24 bcodmo_name String carbonate
attribute CO3_t24 description String Carbonate in vial after 24 hours
attribute CO3_t24 long_name String CO3 T24
attribute CO3_t24 units String micromoles/kilogram (µmol/kg)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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