ERDDAP > info > bcodmo_dataset_738651

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Table 3: Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success, 2011-2012		I   M	background			BCO-DMO	bcodmo_dataset_738651

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Overview:\u00a0 The goal of these laboratory experiments were to determine the degree to which lowered seawater pH would inhibit egg hatching success in marine zooplankton and specifically in the copepod, Calanus finmarchicus.\u00a0 To do this, adult female zooplankton were collected at an open coastal site.\u00a0 The eggs from these females were then collected and incubated for several days in several tanks of seawater bubbled with premixed gas at a specific CO2\u00a0content.\u00a0 The proportion of eggs which hatched were determined in replicate.\u00a0 While the eggs were being incubated, waters in each tank were monitored with sensors for temperature, salinity, pH, and dissolved oxygen.\u00a0 Once or twice during an experiment, 2 L of seawater were withdrawn for measurement of the carbonate system parameters (salinity, alkalinity, total carbon dioxide) and nutrient concentrations.\u00a0 From these high-precision carbonate measurements, the total pH and other carbonate system parameters were calculated.\u00a0 Thus, inhibition of hatching success was related to the pH of the tanks. Egg Collection:\u00a0 Zooplankton were collected from an open coastal site\u00a0 (43.7474 N, 69.5010 W) nearby the University of Maine's Darling Marine Center, using a 1-m 300 m mesh ring net towed from 100 m to the surface.\u00a0 Sufficient quantities of seawater was also collected from 30-40 m depth for most experiments.\u00a0 The copepod, Calanus finmarchicus, was studied in experiments 1-3 and 6-13, while the euphausid, Meganyctiphanes norvegica, was used in experiments 4 and 5.\u00a0 Females were separated from the catch and allowed to release their eggs for about a day.\u00a0 On occasional experiments, insufficient numbers of females were collected to supply the numbers of eggs required in an experiment.\u00a0 In this case, C. finmarchicus eggs from females maintained in the laboratory were used instead.\u00a0 These females were maintained at 6C and were fed twice daily at concentrations of >500 g C L-1\u00a0with an equal mixture of Gymnodinium sp., Rhodomonas sp. and Oxyrrhis marina.\u00a0 For experiments with the laboratory- maintained females, waters used in the experimental tanks were those pumped from the Marine Center's dock into the marine aquarium building, which housed our laboratory.\u00a0 The estuary's seawater was only slightly less saline (salinities of 30.2-31.1) than the open coastal waters (31.1-32.4).\u00a0 All seawaters used were filtered through 1.0 m filters. Incubations at differing pH:\u00a0 After obtaining sufficient numbers of eggs, the eggs were separated in replicate Petri dishes with 30 eggs per dish.\u00a0 The dishes were specially modified with Nitex screen to allow exchange between the interior of the dish and the surrounding seawater.\u00a0 Between 3 and 5 tanks containing 12.5 L of seawater had been bubbled with specialty-purchased gas mixtures of 20% oxygen, a preselected CO2\u00a0concentration (between 913 and 15,290 ppm), and the remainder nitrogen.\u00a0 One experiment (experiment 7) used two gases to reach a very low pH, one gas cylinder containing 50,000 ppm CO2\u00a0in N2, and one containing 913 ppm CO2\u00a0in 20% O2\u00a0and the remainder N2.\u00a0 In this tank, the dissolved oxygen content in the tank remained about the same level as the other tanks in the experiment, above 70% of saturation.\u00a0 Each experiment was begun by placing up to 8 replicate Petri dishes with the eggs into each tank.\u00a0 The tanks were maintained in this state for about 24 hours past the hatching time determined by McLaren et al. (1969) for the particular temperature.\u00a0 The experiment ended by removing the replicate Petri dishes and manually counting the number of nauplii contained within each petri dish.\u00a0 Hatching success was the percentage of nauplii counted relative to the number of eggs initially in the dish.
attribute	NC_GLOBAL	awards_0_award_nid	String	54712
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1041081
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1041081
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success OCEAN PH AFFECTS CALANUS EGGS - TABLE 3, Preziosi, et al (2017) Mar. Bio. DOI 10.1007/s00227-017-3243-5 PI's: J. Christensen (Green Eyes LLC), J. Runge (GMRI) version: 2018-06-13
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-06-18T14:28:18Z
attribute	NC_GLOBAL	date_modified	String	2019-12-04T15:40:37Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.738651.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/738651
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Adapted so that the millivolt out was logged by computer through a 14 bit A to D converter. The electrode was an Orion Ross ultra semi-micro glass electrode model 8103-BNUWP.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	738657
attribute	NC_GLOBAL	instruments_0_description	String	An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms) This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	681
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Corning model 109
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to count eggs and nauplii.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	738825
attribute	NC_GLOBAL	instruments_1_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	708
attribute	NC_GLOBAL	keywords	String	average, AVERAGE_PHTTL, AVERAGE_TEMP, bco, bco-dmo, biological, chemical, data, dataset, dmo, duration, erddap, experiment, hs1, hs2, hs3, hs4, hs5, hs6, hs7, hs8, management, oceanography, office, phttl, preliminary, source, species, startdate, tank, temperature, time
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/738651/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/738651
attribute	NC_GLOBAL	param_mapping	String	{'738651': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/738651/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Green Eyes LLC
attribute	NC_GLOBAL	people_0_person_name	String	John P Christensen
attribute	NC_GLOBAL	people_0_person_nid	String	51603
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Gulf of Maine Research Institute
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	GMRI
attribute	NC_GLOBAL	people_1_person_name	String	Jeffrey A. Runge
attribute	NC_GLOBAL	people_1_person_nid	String	50905
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_2_person_nid	String	50396
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	OA Calanus Survival
attribute	NC_GLOBAL	projects_0_acronym	String	OA Calanus Survival
attribute	NC_GLOBAL	projects_0_description	String	The project description is a modification of the original NSF award abstract. This research project is part of the larger NSF funded CRI-OA collaborative research initiative and was funded as an Ocean Acidification-Category 1, 2010 award. While attention concerning impacts of predicted acidification of the world's oceans has focused on calcifying organisms, non-calcifying plankton may also be vulnerable. In this project, the investigator will evaluate the potential for impacts of ocean acidification on the reproductive success of three species of planktonic copepods in the genus Calanus that are prominent in high latitude oceans. C. finmarchicus dominates the mesozooplankton biomass across much of the coastal and deep North Atlantic Ocean. C. glacialis and the larger C. hyperboreus are among the most abundant planktonic copepods in the Arctic Ocean. Previous research showed that hatching success of C. finmarchicus eggs was severely inhibited by increased CO2 and lower pH in seawater, but only tested at an extreme level. Preliminary results in the investigator's laboratory indicate that hatching success of C. finmarchicus is substantially reduced at increased seawater CO2 concentrations corresponding to pH levels between 7.9 and 7.5. Predictions of likely decline of surface pH levels to 7.7-7.8 over the next century raise questions about impacts on Calanus population dynamics if these preliminary results are confirmed. C. finmarchicus, for example, is presently at the southern edge of its range in the Gulf of Maine. The combination of higher surface layer temperature and lower pH may inhibit reproductive success during the late summer/fall bloom, which the PI hypothesize is critical to sustain the overwintering stock in this region. The investigators will collect C. finmarchicus females from the Gulf of Maine and, with the assistance of Canadian colleagues, C. glacialis and C. hyperboreus females from the deep lower St. Lawrence Estuary. They will conduct laboratory experiments in which hatching success, development and growth of Calanus nauplius stages are measured in controls of natural seawater and at a series of treatments in which CO2 concentrations, pH and temperature are rigorously controlled to represent possible future states of the northern ocean. The investigators will measure present surface and deep pCO2 and pH across the Gulf of Maine, including its deep basins, during a research cruise. The study will evaluate the hypothesis that predicted levels of CO2 increase in the northern ocean will impact population dynamics of the Calanus species. Using the results from the research cruise and a recently developed 1-D, Individual-Based life cycle model, the PI will explore in detail scenarios of impact of higher temperature and lower surface and deep pH on population dynamics of C. finmarchicus in the Gulf of Maine. The lipid-rich Calanus species are considered key intermediary links between primary production and higher trophic levels in North Atlantic and Arctic Ocean food webs. Impacts of higher surface temperature and lower pH on reproductive success may potentially lead to profound changes in energy transfer and structure of pelagic ecosystems in the northern oceans. In the Gulf of Maine, C. finmarchicus serves as primary prey for herring, sand lance, and mackerel, as well as the endangered northern right whale, warranting thorough evaluation of ocean acidification effects on its population dynamics.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-10
attribute	NC_GLOBAL	projects_0_geolocation	String	Gulf of Maine
attribute	NC_GLOBAL	projects_0_name	String	Ocean Acidification-Category 1- Impact of ocean acidification on survival of early life stages of planktonic copepods in the genus Calanus in the northern
attribute	NC_GLOBAL	projects_0_project_nid	String	2184
attribute	NC_GLOBAL	projects_0_start_date	String	2010-11
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset reports hatching success for eggs of Calanus finmarchicus and Meganyctiphanes norvegica. Average temperature and pH are reported as well as hatching success for each replicate dish of eggs. Results are published in Preziosi et al (2017), Table 3.
attribute	NC_GLOBAL	title	String	Table 3: Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success, 2011-2012
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	EXPERIMENT		byte
attribute	EXPERIMENT	_FillValue	byte	127
attribute	EXPERIMENT	actual_range	byte	1, 13
attribute	EXPERIMENT	bcodmo_name	String	exp_id
attribute	EXPERIMENT	description	String	Number of the experiment
attribute	EXPERIMENT	long_name	String	EXPERIMENT
attribute	EXPERIMENT	units	String	#
variable	STARTDATE		String
attribute	STARTDATE	bcodmo_name	String	date_start
attribute	STARTDATE	description	String	Date the experiment began in mm/dd/yyyy format.
attribute	STARTDATE	long_name	String	STARTDATE
attribute	STARTDATE	source_name	String	STARTDATE
attribute	STARTDATE	time_precision	String	1970-01-01
attribute	STARTDATE	units	String	unitless
variable	SPECIES		String
attribute	SPECIES	bcodmo_name	String	taxon
attribute	SPECIES	description	String	Name of the species
attribute	SPECIES	long_name	String	SPECIES
attribute	SPECIES	units	String	name
variable	SOURCE		String
attribute	SOURCE	bcodmo_name	String	sample_descrip
attribute	SOURCE	description	String	Freshly caught or lab-maintained females
attribute	SOURCE	long_name	String	SOURCE
attribute	SOURCE	units	String	text
variable	DURATION		short
attribute	DURATION	_FillValue	short	32767
attribute	DURATION	actual_range	short	49, 139
attribute	DURATION	bcodmo_name	String	time_elapsed
attribute	DURATION	description	String	Elapsed time for eggs in the tanks
attribute	DURATION	long_name	String	DURATION
attribute	DURATION	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	DURATION	units	String	hours
variable	TANK		byte
attribute	TANK	_FillValue	byte	127
attribute	TANK	actual_range	byte	1, 6
attribute	TANK	bcodmo_name	String	replicate
attribute	TANK	description	String	Number of the tank
attribute	TANK	long_name	String	TANK
attribute	TANK	units	String	#
variable	AVERAGE_TEMP		float
attribute	AVERAGE_TEMP	_FillValue	float	NaN
attribute	AVERAGE_TEMP	actual_range	float	3.5, 15.73
attribute	AVERAGE_TEMP	bcodmo_name	String	temperature
attribute	AVERAGE_TEMP	description	String	Tank average of temperatures in Table 2
attribute	AVERAGE_TEMP	long_name	String	AVERAGE TEMP
attribute	AVERAGE_TEMP	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	AVERAGE_TEMP	units	String	degrees C
variable	AVERAGE_PHTTL		float
attribute	AVERAGE_PHTTL	_FillValue	float	NaN
attribute	AVERAGE_PHTTL	actual_range	float	6.026, 8.023
attribute	AVERAGE_PHTTL	bcodmo_name	String	pH
attribute	AVERAGE_PHTTL	description	String	Tank average of pHttl in Table 2
attribute	AVERAGE_PHTTL	long_name	String	AVERAGE PHTTL
attribute	AVERAGE_PHTTL	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/
attribute	AVERAGE_PHTTL	units	String	pH units
variable	HS1		float
attribute	HS1	_FillValue	float	NaN
attribute	HS1	actual_range	float	0.0, 100.0
attribute	HS1	bcodmo_name	String	unknown
attribute	HS1	description	String	% of eggs which hatched in replicate 1
attribute	HS1	long_name	String	HS1
attribute	HS1	units	String	percent
variable	HS2		float
attribute	HS2	_FillValue	float	NaN
attribute	HS2	actual_range	float	0.0, 100.0
attribute	HS2	bcodmo_name	String	unknown
attribute	HS2	description	String	% of eggs which hatched in replicate 2
attribute	HS2	long_name	String	HS2
attribute	HS2	units	String	percent
variable	HS3		float
attribute	HS3	_FillValue	float	NaN
attribute	HS3	actual_range	float	-99.0, 93.3
attribute	HS3	bcodmo_name	String	unknown
attribute	HS3	description	String	% of eggs which hatched in replicate 3
attribute	HS3	long_name	String	HS3
attribute	HS3	units	String	percent
variable	HS4		float
attribute	HS4	_FillValue	float	NaN
attribute	HS4	actual_range	float	-99.0, 90.0
attribute	HS4	bcodmo_name	String	unknown
attribute	HS4	description	String	% of eggs which hatched in replicate 4
attribute	HS4	long_name	String	HS4
attribute	HS4	units	String	percent
variable	HS5		float
attribute	HS5	_FillValue	float	NaN
attribute	HS5	actual_range	float	-99.0, 92.9
attribute	HS5	bcodmo_name	String	unknown
attribute	HS5	description	String	% of eggs which hatched in replicate 5
attribute	HS5	long_name	String	HS5
attribute	HS5	units	String	percent
variable	HS6		float
attribute	HS6	_FillValue	float	NaN
attribute	HS6	actual_range	float	-99.0, 100.0
attribute	HS6	bcodmo_name	String	unknown
attribute	HS6	description	String	% of eggs which hatched in replicate 6
attribute	HS6	long_name	String	HS6
attribute	HS6	units	String	percent
variable	HS7		float
attribute	HS7	_FillValue	float	NaN
attribute	HS7	actual_range	float	-99.0, 56.7
attribute	HS7	bcodmo_name	String	unknown
attribute	HS7	description	String	% of eggs which hatched in replicate 7
attribute	HS7	long_name	String	HS7
attribute	HS7	units	String	percent
variable	HS8		float
attribute	HS8	_FillValue	float	NaN
attribute	HS8	actual_range	float	-99.0, 80.0
attribute	HS8	bcodmo_name	String	unknown
attribute	HS8	description	String	% of eggs which hatched in replicate 8
attribute	HS8	long_name	String	HS8
attribute	HS8	units	String	percent

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.