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Institution Dataset ID
     data   graph     files  public Table 3: Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success, 2011-2012    ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_738651

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Overview:\u00a0 The goal of these laboratory experiments were to determine the
degree to which lowered seawater pH would inhibit egg hatching success in
marine zooplankton and specifically in the copepod, Calanus
finmarchicus.\u00a0 To do this, adult female zooplankton were collected at an
open coastal site.\u00a0 The eggs from these females were then collected and
incubated for several days in several tanks of seawater bubbled with premixed
gas at a specific CO2\u00a0content.\u00a0 The proportion of eggs which hatched
were determined in replicate.\u00a0 While the eggs were being incubated,
waters in each tank were monitored with sensors for temperature, salinity, pH,
and dissolved oxygen.\u00a0 Once or twice during an experiment, 2 L of
seawater were withdrawn for measurement of the carbonate system parameters
(salinity, alkalinity, total carbon dioxide) and nutrient
concentrations.\u00a0 From these high-precision carbonate measurements, the
total pH and other carbonate system parameters were calculated.\u00a0 Thus,
inhibition of hatching success was related to the pH of the tanks.

Egg Collection:\u00a0 Zooplankton were collected from an open coastal
site\u00a0 (43.7474 N, 69.5010 W) nearby the University of Maine's Darling
Marine Center, using a 1-m 300 m mesh ring net towed from 100 m to the
surface.\u00a0 Sufficient quantities of seawater was also collected from 30-40
m depth for most experiments.\u00a0 The copepod, Calanus finmarchicus, was
studied in experiments 1-3 and 6-13, while the euphausid, Meganyctiphanes
norvegica, was used in experiments 4 and 5.\u00a0 Females were separated from
the catch and allowed to release their eggs for about a day.\u00a0 On
occasional experiments, insufficient numbers of females were collected to
supply the numbers of eggs required in an experiment.\u00a0 In this case, C.
finmarchicus eggs from females maintained in the laboratory were used
instead.\u00a0 These females were maintained at 6C and were fed twice daily at
concentrations of >500 g C L-1\u00a0with an equal mixture of Gymnodinium sp.,
Rhodomonas sp. and Oxyrrhis marina.\u00a0 For experiments with the laboratory-
maintained females, waters used in the experimental tanks were those pumped
from the Marine Center's dock into the marine aquarium building, which housed
our laboratory.\u00a0 The estuary's seawater was only slightly less saline
(salinities of 30.2-31.1) than the open coastal waters (31.1-32.4).\u00a0 All
seawaters used were filtered through 1.0 m filters.

Incubations at differing pH:\u00a0 After obtaining sufficient numbers of eggs,
the eggs were separated in replicate Petri dishes with 30 eggs per dish.\u00a0
The dishes were specially modified with Nitex screen to allow exchange between
the interior of the dish and the surrounding seawater.\u00a0 Between 3 and 5
tanks containing 12.5 L of seawater had been bubbled with specialty-purchased
gas mixtures of 20% oxygen, a preselected CO2\u00a0concentration (between 913
and 15,290 ppm), and the remainder nitrogen.\u00a0 One experiment (experiment
7) used two gases to reach a very low pH, one gas cylinder containing 50,000
ppm CO2\u00a0in N2, and one containing 913 ppm CO2\u00a0in 20% O2\u00a0and the
remainder N2.\u00a0 In this tank, the dissolved oxygen content in the tank
remained about the same level as the other tanks in the experiment, above 70%
of saturation.\u00a0 Each experiment was begun by placing up to 8 replicate
Petri dishes with the eggs into each tank.\u00a0 The tanks were maintained in
this state for about 24 hours past the hatching time determined by McLaren et
al. (1969) for the particular temperature.\u00a0 The experiment ended by
removing the replicate Petri dishes and manually counting the number of
nauplii contained within each petri dish.\u00a0 Hatching success was the
percentage of nauplii counted relative to the number of eggs initially in the
attribute NC_GLOBAL awards_0_award_nid String 54712
attribute NC_GLOBAL awards_0_award_number String OCE-1041081
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1041081 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success
OCEAN PH AFFECTS CALANUS EGGS - TABLE 3, Preziosi, et al (2017) Mar. Bio. DOI 10.1007/s00227-017-3243-5
PI's: J. Christensen (Green Eyes LLC), J. Runge (GMRI)
version: 2018-06-13
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-06-18T14:28:18Z
attribute NC_GLOBAL date_modified String 2019-12-04T15:40:37Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.738651.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/738651 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Benchtop pH Meter
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Adapted so that the millivolt out was logged by computer through a 14 bit A to D converter. The electrode was an Orion Ross ultra semi-micro glass electrode model 8103-BNUWP.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 738657
attribute NC_GLOBAL instruments_0_description String An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute NC_GLOBAL instruments_0_instrument_name String Benchtop pH Meter
attribute NC_GLOBAL instruments_0_instrument_nid String 681
attribute NC_GLOBAL instruments_0_supplied_name String Corning model 109
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to count eggs and nauplii.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 738825
attribute NC_GLOBAL instruments_1_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Microscope-Optical
attribute NC_GLOBAL instruments_1_instrument_nid String 708
attribute NC_GLOBAL keywords String average, AVERAGE_PHTTL, AVERAGE_TEMP, bco, bco-dmo, biological, chemical, data, dataset, dmo, duration, erddap, experiment, hs1, hs2, hs3, hs4, hs5, hs6, hs7, hs8, management, oceanography, office, phttl, preliminary, source, species, startdate, tank, temperature, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/738651/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/738651 (external link)
attribute NC_GLOBAL param_mapping String {'738651': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/738651/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Green Eyes LLC
attribute NC_GLOBAL people_0_person_name String John P Christensen
attribute NC_GLOBAL people_0_person_nid String 51603
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Gulf of Maine Research Institute
attribute NC_GLOBAL people_1_affiliation_acronym String GMRI
attribute NC_GLOBAL people_1_person_name String Jeffrey A. Runge
attribute NC_GLOBAL people_1_person_nid String 50905
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String OA Calanus Survival
attribute NC_GLOBAL projects_0_acronym String OA Calanus Survival
attribute NC_GLOBAL projects_0_description String The project description is a modification of the original NSF award abstract.
This research project is part of the larger NSF funded CRI-OA collaborative research initiative and was funded as an Ocean Acidification-Category 1, 2010 award. While attention concerning impacts of predicted acidification of the world's oceans has focused on calcifying organisms, non-calcifying plankton may also be vulnerable. In this project, the investigator will evaluate the potential for impacts of ocean acidification on the reproductive success of three species of planktonic copepods in the genus Calanus that are prominent in high latitude oceans. C. finmarchicus dominates the mesozooplankton biomass across much of the coastal and deep North Atlantic Ocean. C. glacialis and the larger C. hyperboreus are among the most abundant planktonic copepods in the Arctic Ocean. Previous research showed that hatching success of C. finmarchicus eggs was severely inhibited by increased CO2 and lower pH in seawater, but only tested at an extreme level. Preliminary results in the investigator's laboratory indicate that hatching success of C. finmarchicus is substantially reduced at increased seawater CO2 concentrations corresponding to pH levels between 7.9 and 7.5. Predictions of likely decline of surface pH levels to 7.7-7.8 over the next century raise questions about impacts on Calanus population dynamics if these preliminary results are confirmed. C. finmarchicus, for example, is presently at the southern edge of its range in the Gulf of Maine. The combination of higher surface layer temperature and lower pH may inhibit reproductive success during the late summer/fall bloom, which the PI hypothesize is critical to sustain the overwintering stock in this region. The investigators will collect C. finmarchicus females from the Gulf of Maine and, with the assistance of Canadian colleagues, C. glacialis and C. hyperboreus females from the deep lower St. Lawrence Estuary. They will conduct laboratory experiments in which hatching success, development and growth of Calanus nauplius stages are measured in controls of natural seawater and at a series of treatments in which CO2 concentrations, pH and temperature are rigorously controlled to represent possible future states of the northern ocean. The investigators will measure present surface and deep pCO2 and pH across the Gulf of Maine, including its deep basins, during a research cruise. The study will evaluate the hypothesis that predicted levels of CO2 increase in the northern ocean will impact population dynamics of the Calanus species. Using the results from the research cruise and a recently developed 1-D, Individual-Based life cycle model, the PI will explore in detail scenarios of impact of higher temperature and lower surface and deep pH on population dynamics of C. finmarchicus in the Gulf of Maine.
The lipid-rich Calanus species are considered key intermediary links between primary production and higher trophic levels in North Atlantic and Arctic Ocean food webs. Impacts of higher surface temperature and lower pH on reproductive success may potentially lead to profound changes in energy transfer and structure of pelagic ecosystems in the northern oceans. In the Gulf of Maine, C. finmarchicus serves as primary prey for herring, sand lance, and mackerel, as well as the endangered northern right whale, warranting thorough evaluation of ocean acidification effects on its population dynamics.
attribute NC_GLOBAL projects_0_end_date String 2013-10
attribute NC_GLOBAL projects_0_geolocation String Gulf of Maine
attribute NC_GLOBAL projects_0_name String Ocean Acidification-Category 1- Impact of ocean acidification on survival of early life stages of planktonic copepods in the genus Calanus in the northern
attribute NC_GLOBAL projects_0_project_nid String 2184
attribute NC_GLOBAL projects_0_start_date String 2010-11
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset reports hatching success for eggs of Calanus finmarchicus and Meganyctiphanes norvegica. Average temperature and pH are reported as well as hatching success for each replicate dish of eggs. Results are published in Preziosi et al (2017), Table 3.
attribute NC_GLOBAL title String Table 3: Calanus finmarchicus and Meganyctiphanes norvegica egg hatching success, 2011-2012
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable EXPERIMENT   byte  
attribute EXPERIMENT _FillValue byte 127
attribute EXPERIMENT actual_range byte 1, 13
attribute EXPERIMENT bcodmo_name String exp_id
attribute EXPERIMENT description String Number of the experiment
attribute EXPERIMENT long_name String EXPERIMENT
attribute EXPERIMENT units String #
variable STARTDATE   String  
attribute STARTDATE bcodmo_name String date_start
attribute STARTDATE description String Date the experiment began in mm/dd/yyyy format.
attribute STARTDATE long_name String STARTDATE
attribute STARTDATE source_name String STARTDATE
attribute STARTDATE time_precision String 1970-01-01
attribute STARTDATE units String unitless
variable SPECIES   String  
attribute SPECIES bcodmo_name String taxon
attribute SPECIES description String Name of the species
attribute SPECIES long_name String SPECIES
attribute SPECIES units String name
variable SOURCE   String  
attribute SOURCE bcodmo_name String sample_descrip
attribute SOURCE description String Freshly caught or lab-maintained females
attribute SOURCE long_name String SOURCE
attribute SOURCE units String text
variable DURATION   short  
attribute DURATION _FillValue short 32767
attribute DURATION actual_range short 49, 139
attribute DURATION bcodmo_name String time_elapsed
attribute DURATION description String Elapsed time for eggs in the tanks
attribute DURATION long_name String DURATION
attribute DURATION nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute DURATION units String hours
variable TANK   byte  
attribute TANK _FillValue byte 127
attribute TANK actual_range byte 1, 6
attribute TANK bcodmo_name String replicate
attribute TANK description String Number of the tank
attribute TANK long_name String TANK
attribute TANK units String #
variable AVERAGE_TEMP   float  
attribute AVERAGE_TEMP _FillValue float NaN
attribute AVERAGE_TEMP actual_range float 3.5, 15.73
attribute AVERAGE_TEMP bcodmo_name String temperature
attribute AVERAGE_TEMP description String Tank average of temperatures in Table 2
attribute AVERAGE_TEMP long_name String AVERAGE TEMP
attribute AVERAGE_TEMP nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute AVERAGE_TEMP units String degrees C
variable AVERAGE_PHTTL   float  
attribute AVERAGE_PHTTL _FillValue float NaN
attribute AVERAGE_PHTTL actual_range float 6.026, 8.023
attribute AVERAGE_PHTTL bcodmo_name String pH
attribute AVERAGE_PHTTL description String Tank average of pHttl in Table 2
attribute AVERAGE_PHTTL long_name String AVERAGE PHTTL
attribute AVERAGE_PHTTL nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute AVERAGE_PHTTL units String pH units
variable HS1   float  
attribute HS1 _FillValue float NaN
attribute HS1 actual_range float 0.0, 100.0
attribute HS1 bcodmo_name String unknown
attribute HS1 description String % of eggs which hatched in replicate 1
attribute HS1 long_name String HS1
attribute HS1 units String percent
variable HS2   float  
attribute HS2 _FillValue float NaN
attribute HS2 actual_range float 0.0, 100.0
attribute HS2 bcodmo_name String unknown
attribute HS2 description String % of eggs which hatched in replicate 2
attribute HS2 long_name String HS2
attribute HS2 units String percent
variable HS3   float  
attribute HS3 _FillValue float NaN
attribute HS3 actual_range float -99.0, 93.3
attribute HS3 bcodmo_name String unknown
attribute HS3 description String % of eggs which hatched in replicate 3
attribute HS3 long_name String HS3
attribute HS3 units String percent
variable HS4   float  
attribute HS4 _FillValue float NaN
attribute HS4 actual_range float -99.0, 90.0
attribute HS4 bcodmo_name String unknown
attribute HS4 description String % of eggs which hatched in replicate 4
attribute HS4 long_name String HS4
attribute HS4 units String percent
variable HS5   float  
attribute HS5 _FillValue float NaN
attribute HS5 actual_range float -99.0, 92.9
attribute HS5 bcodmo_name String unknown
attribute HS5 description String % of eggs which hatched in replicate 5
attribute HS5 long_name String HS5
attribute HS5 units String percent
variable HS6   float  
attribute HS6 _FillValue float NaN
attribute HS6 actual_range float -99.0, 100.0
attribute HS6 bcodmo_name String unknown
attribute HS6 description String % of eggs which hatched in replicate 6
attribute HS6 long_name String HS6
attribute HS6 units String percent
variable HS7   float  
attribute HS7 _FillValue float NaN
attribute HS7 actual_range float -99.0, 56.7
attribute HS7 bcodmo_name String unknown
attribute HS7 description String % of eggs which hatched in replicate 7
attribute HS7 long_name String HS7
attribute HS7 units String percent
variable HS8   float  
attribute HS8 _FillValue float NaN
attribute HS8 actual_range float -99.0, 80.0
attribute HS8 bcodmo_name String unknown
attribute HS8 description String % of eggs which hatched in replicate 8
attribute HS8 long_name String HS8
attribute HS8 units String percent

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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