ERDDAP > info > bcodmo_dataset_764780

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Partition_coefficients_for_COC] - Partition coefficients of the radionuclide into colloidal organic matter from seawater of the west Pacific Ocean and the northern Gulf of Mexico (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)		I   M	background			BCO-DMO	bcodmo_dataset_764780

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Activity concentrations of 234Th, 233Pa, 210Pb, and 7Be were measured by counting the gamma decay energies at 63.5 keV, 312 keV, 46.5 keV, and 477.6 keV, respectively, on a Canberra ultrahigh purity germanium well detector. The 210Po activity was analyzed by liquid scintillation counting (Beckman Model 8100 Liquid Scintillation Counter). Concentrations of total carbohydrate (TCHO) were determined by the TPTZ (2, 4, 6-tripyridyl-s-triazine) method using glucose as the standard and [Hung and Santschi, 2001]. Protein content was determined using a modified Lowry protein assay, using bovine serum albumin as the standard (Pierce, Thermo Scientific).\u00a0 Elemental contents of carbon (C) and nitrogen (N), were determined by a Perkin Elmer CHN 2400 analyzer, using cysteine (29.99% C, 11.67% N) as a standard.\u00a0 In order to examine the specific binding ligands to five different radionuclides in the marine colloids and diatom culture-derived EPS, radiolabeled biopolymers (E1, E3, C9 and C11; suitable due to their high OC content and available sample amount) were subjected to Isoelectric Focusing (IEF) separation with a Gel Electrophoresis apparatus (Amersham Biosciences, Multiphor Electrophoresis System). Briefly, radiolabeled biopolymers and a 140 \u03bcL of rehydration solution were loaded onto an IPG strip (GE Healthcare Immobiline\u2122 Drystrip, pH 3\u201310, 11 cm) and were re-swelled overnight. Afterwards, the strip was loaded into the device for isoelectric focusing for 17.5 h. The strip was then cut into eleven 1 cm-pieces and followed by 1% SDS extraction overnight. Activity concentrations of the five radionuclides in each fraction were subsequently analyzed. Due to the limited amount of each strip fraction, only selected chemical components (TCHO, Proteins and Fe) in individual fractions were characterized by methods described above. To avoid any interference from any background contamination, SDS extractants underwent diafiltration (desalting) using 1 kDa cutoff Microsep\u2122 centrifugal devices (Pall Life Sciences) using ultra pure Milli-Q water for at least three times. \u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	735995
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1356453
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1356453
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	The partition coefficients of the radionuclide into colloidal organic matter, logKc values. PI: Peter H. Santschi Version: 2019-04-11
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-04-11T15:30:02Z
attribute	NC_GLOBAL	date_modified	String	2019-04-11T19:55:18Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.764780.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/764780
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	CHN_EA
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Elemental contents of carbon (C) and nitrogen (N), were determined by a Perkin Elmer CHN 2400 analyzer, using cysteine (29.99% C, 11.67% N) as a standard.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	764856
attribute	NC_GLOBAL	instruments_0_description	String	A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	CHN Elemental Analyzer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	625
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Perkin Elmer CHN 2400 analyzer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	In order to examine the specific binding ligands to five different radionuclides in the marine colloids and diatom culture-derived EPS, radiolabeled biopolymers (E1, E3, C9 and C11; suitable due to their high OC content and available sample amount) were subjected to Isoelectric Focusing (IEF) separation with a Gel Electrophoresis apparatus (Amersham Biosciences, Multiphor Electrophoresis System).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	764857
attribute	NC_GLOBAL	instruments_1_description	String	General term for an apparatus used in clinical and research laboratories to separate charged colloidal particles (or molecules) of varying size through a medium by applying an electric field.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Electrophoresis Chamber
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	471592
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Amersham Biosciences, Multiphor Electrophoresis System
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, calculation, calculation_type, chemical, compound, compound_for_ratio, data, dataset, dmo, erddap, log, LogK, LogK_radionuclide, management, oceanography, office, pcnt, preliminary, radionuclide, ratio, ratio_to_OC_pcnt, type
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/764780/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/764780
attribute	NC_GLOBAL	param_mapping	String	{'764780': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/764780/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_0_person_name	String	Peter Santschi
attribute	NC_GLOBAL	people_0_person_nid	String	735998
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_1_person_name	String	Antonietta Quigg
attribute	NC_GLOBAL	people_1_person_nid	String	736000
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_2_person_name	String	Kathleen Schwehr
attribute	NC_GLOBAL	people_2_person_nid	String	736002
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_3_person_name	String	Chen Xu
attribute	NC_GLOBAL	people_3_person_nid	String	736004
attribute	NC_GLOBAL	people_3_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_3_role_type	String	originator
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Mathew Biddle
attribute	NC_GLOBAL	people_4_person_nid	String	708682
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_acronym	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Particle-associated natural radioisotopes are transported to the ocean floor mostly via silica and carbonate ballasted particles, allowing their use as tracers for particle transport. Th(IV), Pa (IV,V), Po(IV), Pb(II) and Be(II) radionuclides are important proxies in oceanographic investigations, used for tracing particle and colloid cycling, estimating export fluxes of particulate organic carbon, tracing air-sea exchange, paleoproductivity, and/or ocean circulation in paleoceanographic studies. Even though tracer approaches are considered routine, there are cases where data interpretation or validity has become controversial, largely due to uncertainties about inorganic proxies and organic carrier molecules. Recent studies showed that cleaned diatom frustules and pure silica particles, sorb natural radionuclides to a much lower extent (by 1-2 orders of magnitude) than whole diatom cells (with or without shells). Phytoplankton that build siliceous or calcareous shells, such as the diatoms and coccolithophores, are assembled via bio-mineralization processes using biopolymers as nanoscale templates. These templates could serve as possible carriers for radionuclides and stable metals. In this project, a research team at the Texas A & M University at Galveston hypothesize that radionuclide sorption is controlled by selective biopolymers that are associated with biogenic opal (diatoms), CaCO3 (coccolithophores) and the attached exopolymeric substances (EPS), rather than to pure mineral phase. To pursue this idea, the major objectives of their research will include separation, identification and molecular-level characterization of the individual biopolymers (e.g., polysaccharides, uronic acids, proteins, hydroquinones, hydroxamate siderophores, etc.) that are responsible for binding different radionuclides (Th, Pa, Pb, Po and Be) attached to cells or in the matrix of biogenic opal or CaCO3 as well as attached EPS mixture, in laboratory grown diatom and coccolithophore cultures. Laboratory-scale radiolabeling experiments will be conducted, and different separation techniques and characterization techniques will be applied. Intellectual Merit : It is expected that this study will help elucidate the molecular basis of the templated growth of diatoms and coccoliths, EPS and their role in scavenging natural radionuclides in the ocean, and help resolve debates on the oceanographic tracer applications of different natural radioisotopes (230,234Th, 231Pa, 210Po, 210Pb and 7,10Be). The proposed interdisciplinary research project will require instrumental approaches for molecular-level characterization of these radionuclides associated carrier molecules. Broader Impacts: The results of this study will be relevant for understanding biologically mediated ocean scavenging of radionuclides by diatoms and coccoliths which is important for carbon cycling in the ocean, and will contribute to improved interpretation of data obtained by field studies especially through the GEOTRACES program. This new program will enhance training programs at TAMUG for postdocs, graduate and undergraduate students. Lastly, results will be integrated in college courses and out-reach activities at Texas A&M University, including NSF-REU, Sea Camp, Elder Hostel and exhibits at the local science fair and interaction with its after-school program engaging Grade 9-12 students from groups traditionally underrepresented.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-02
attribute	NC_GLOBAL	projects_0_name	String	Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores
attribute	NC_GLOBAL	projects_0_project_nid	String	735996
attribute	NC_GLOBAL	projects_0_start_date	String	2014-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	To study the binding mechanisms of radionuclides to organic moieties in colloidal organic matter (COM),marine colloids (1 kDa\u20130.2 \u03bcm) were isolated by cross-flow ultrafiltration from seawater of the west Pacific Ocean and the northern Gulf of Mexico. For the same purpose, exopolymeric substances (EPS) produced by laboratory cultured diatoms were collected as well. In our study areas, colloidal organic carbon (COC) concentrations ranged from 6.5 to 202 \u03bcg-C/L in the Pacific Ocean, and were 808 \u03bcg-C/L in the Gulf of Mexico. The COM compositions (organic carbon, organic nitrogen, proteins, total hydrolysable amino acids, total polysaccharides, uronic acids, hydroxamate siderophores, hydroquinone) were quantified to examine the relationships between partition coefficients (Kc) of five different radionuclides, 234Th, 233Pa, 210Pb, 210Po and 7Be, and concentration ratios to COC of individual chelating biomolecules that could potentially act as a chelating moiety. The range of partition coefficients (Kc, reported as logKc) of radionuclides between water and the different colloidal materials was 5.12 to 5.85 for 234Th, 5.19 to 6.01 for 233Pa, 4.21 to 4.85 for 210Pb, 4.87 to 5.68 for 210Po, and 4.49 to 4.92 for 7Be, similar to values previously reported for lab and field determinations under different particle concentrations. While any relationship obtained between Kc and abundance of specific moieties could not be taken as proving the existence of colloidal organic binding ligands for the different radionuclides, it could suggest possible organic moieties involved in the scavenging of these natural radionuclides. Together with results from isoelectric focusing of radiolabeled COM, we conclude that binding to different biomolecules is nuclide-specific, with colloidal hydroxamate siderophoric moieties being important for the binding of Th and Pa radionuclides. Hydroquinones/ quinone (HQ/Q) facilitated redox and chelation reactions seem to be involved in the binding of Pa and Be. However, the actual mechanisms are not clear. Individual amino acids, proteins, total polysaccharides and uronic acids did not yield significant relationships with logKc values of the different radionuclides. Nonetheless, our results provide new insights into the relative importance of different potential ligand moieties in COM in the binding and possible scavenging of specific radionuclides in the ocean.
attribute	NC_GLOBAL	title	String	[Partition_coefficients_for_COC] - Partition coefficients of the radionuclide into colloidal organic matter from seawater of the west Pacific Ocean and the northern Gulf of Mexico (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	compound_for_ratio		String
attribute	compound_for_ratio	bcodmo_name	String	unknown
attribute	compound_for_ratio	description	String	compound used in the ratio for ratio_to_OC_pcnt
attribute	compound_for_ratio	long_name	String	Compound For Ratio
attribute	compound_for_ratio	units	String	unitless
variable	ratio_to_OC_pcnt		float
attribute	ratio_to_OC_pcnt	_FillValue	float	NaN
attribute	ratio_to_OC_pcnt	actual_range	float	0.02, 2.04
attribute	ratio_to_OC_pcnt	bcodmo_name	String	unknown
attribute	ratio_to_OC_pcnt	description	String	Carbon normalized ratio of the compound in compound_for_ratio repreented as a percent
attribute	ratio_to_OC_pcnt	long_name	String	Ratio To OC Pcnt
attribute	ratio_to_OC_pcnt	units	String	unitless (percent)
variable	LogK_radionuclide		String
attribute	LogK_radionuclide	bcodmo_name	String	unknown
attribute	LogK_radionuclide	description	String	the radionuclide for the partition coefficient
attribute	LogK_radionuclide	long_name	String	Log K Radionuclide
attribute	LogK_radionuclide	units	String	unitless
variable	LogK		float
attribute	LogK	_FillValue	float	NaN
attribute	LogK	actual_range	float	4.49, 6.01
attribute	LogK	bcodmo_name	String	unknown
attribute	LogK	description	String	partition coefficient of the radionuclide in LogK_radionuclide
attribute	LogK	long_name	String	Log K
attribute	LogK	units	String	logKc
variable	calculation_type		String
attribute	calculation_type	bcodmo_name	String	unknown
attribute	calculation_type	description	String	the procedure for calculating LogK
attribute	calculation_type	long_name	String	Calculation Type
attribute	calculation_type	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.